Reviewed, UniProtKB/Swiss-Prot P60363 (HPPA1_RHOPA)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	RPA2731

Organism

Rhodopseudomonas palustris [Complete proteome] [HAMAP]

Taxonomic identifier

1076 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217028
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	62 – 82	21	Potential
	Transmembrane	83 – 103	21	Potential
	Transmembrane	129 – 149	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	231 – 251	21	Potential
	Transmembrane	263 – 283	21	Potential
	Transmembrane	300 – 320	21	Potential
	Transmembrane	330 – 350	21	Potential
	Transmembrane	393 – 413	21	Potential
	Transmembrane	467 – 487	21	Potential
	Transmembrane	516 – 536	21	Potential
	Transmembrane	585 – 605	21	Potential
	Transmembrane	616 – 636	21	Potential
	Transmembrane	685 – 705	21	Potential
Sites
	Site	466	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60363-1 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: FB4332D4F65F668E	FASTA	706	71,952
	10 20 30 40 50 60 MTALWVIVLC GALSIVYAIW ATSSVLAADQ GNARMQEIAG AVREGAQAYL KRQYMTIAIV 70 80 90 100 110 120 GVVIFALLAY FLGILVAIGF AIGAILSGAA GFIGMNVSVR ANVRTAQAAT TSLAGGLELA 130 140 150 160 170 180 FKAGAITGLL VAGLALLGVT LYFIYLIHFA GLQANSRTVV DALVALGFGA SLISIFARLG 190 200 210 220 230 240 GGIFTKGADV GGDLVGKVEA GIPEDDPRNP ATIADNVGDN VGDCAGMAAD LFETYAVTAV 250 260 270 280 290 300 ATMVLAAIFF GSATPDQLQS VMTLPLAIGG ICILTSIAGT FFVKLGASQS IMGALYKGLI 310 320 330 340 350 360 ATGVLSLVGV GVVIHQLIGF GPLPGVSYTG LALFECGIVG LAVTGLIIWI TEYYTGTDFR 370 380 390 400 410 420 PVKSIAQASV TGHGTNVIQG LAISMESTAL PAIVIIAGIL ITYSLAGLFG IAIATTTMLA 430 440 450 460 470 480 LAGMIVALDA FGPVTDNAGG IAEMAGLPKE VRKSTDALDA VGNTTKAVTK GYAIGSAGLG 490 500 510 520 530 540 ALVLFAAYNE DLKFFIAQKS PYFVGVAPDF SLNNPYVVVG LLFGGLLPYL FGAMGMTAVG 550 560 570 580 590 600 RAAGAIVEEV RRQFREKPGI MKGTDKPDYG KAVDLLTKAA IKEMIIPSLL PVLSPIFVYF 610 620 630 640 650 660 AIYAIAGGGA AGKSAAFSAV GAMLLGVIVT GLFVAISMTS GGGAWDNAKK YIEDGHHGGK 670 680 690 700 GSDAHKAAVT GDTVGDPYKD TAGPAVNPMI KITNIVALLL LAILAH

References

[1]

"Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris." Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L., Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R., Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C., Harrison F.H., Gibson J., Harwood C.S. Nat. Biotechnol. 22:55-61(2004) [PubMed: 14704707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-98 / CGA009.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX572601 Genomic DNA. Translation: CAE28173.1.
RefSeq	NP_948074.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2693498.
GenomeReviews	Gene locus RPA2731 in contig BX571963_GR.
KEGG	rpa:RPA2731.
NMPDR	fig|258594.1.peg.2717.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P60363.
OMA	TFGPITD.
Enzyme and pathway databases
BioCyc	RPAL258594:RPA2731-MON.
BRENDA	3.6.1.1. 373.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA1_RHOPA

Accession

Primary (citable) accession number: P60363

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)