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P60355

- MCAT_LACPN

UniProt

P60355 - MCAT_LACPN

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Protein
Manganese catalase
Gene
N/A
Organism
Lactobacillus plantarum
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 H2O2 = O2 + 2 H2O.

Cofactori

Binds 1 calcium ion per subunit.
Binds 2 manganese ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351Manganese 1
Metal bindingi57 – 571Calcium
Metal bindingi61 – 611Calcium
Metal bindingi66 – 661Manganese 1
Metal bindingi66 – 661Manganese 2
Metal bindingi69 – 691Manganese 1
Metal bindingi148 – 1481Manganese 2
Metal bindingi181 – 1811Manganese 2
Metal bindingi218 – 2181Calcium
Metal bindingi220 – 2201Calcium; via carbonyl oxygen
Metal bindingi222 – 2221Calcium; via carbonyl oxygen

GO - Molecular functioni

  1. catalase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, Manganese, Metal-binding

Protein family/group databases

PeroxiBasei3978. LplMnCat01.

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese catalase (EC:1.11.1.6)
Alternative name(s):
Pseudocatalase
OrganismiLactobacillus plantarum
Taxonomic identifieri1590 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421Y → F: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Manganese catalase
PRO_0000096155Add
BLAST

Interactioni

Subunit structurei

Homohexamer.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43
Helixi19 – 3012
Helixi35 – 4814
Helixi53 – 8028
Turni81 – 833
Helixi88 – 936
Helixi97 – 1037
Helixi106 – 1105
Beta strandi113 – 1153
Helixi128 – 1303
Helixi137 – 15923
Helixi165 – 19632
Beta strandi198 – 2014
Turni205 – 2073
Helixi210 – 2123
Beta strandi215 – 2173
Helixi224 – 2285
Beta strandi240 – 2445
Helixi259 – 2613

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKUX-ray1.84A/B/C/D/E/F1-266[»]
1JKVX-ray1.39A/B/C/D/E/F1-266[»]
1O9IX-ray1.33A/B/C/D/E/F1-266[»]
ProteinModelPortaliP60355.
SMRiP60355. Positions 1-266.

Miscellaneous databases

EvolutionaryTraceiP60355.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
3.30.1530.10. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR007760. Mn_catalase.
IPR027407. Mn_catalase_dom2.
[Graphical view]
PfamiPF05067. Mn_catalase. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.

Sequencei

Sequence statusi: Complete.

P60355-1 [UniParc]FASTAAdd to Basket

« Hide

MFKHTRKLQY NAKPDRSDPI MARRLQESLG GQWGETTGMM SYLSQGWAST    50
GAEKYKDLLL DTGTEEMAHV EMISTMIGYL LEDAPFGPED LKRDPSLATT 100
MAGMDPEHSL VHGLNASLNN PNGAAWNAGY VTSSGNLVAD MRFNVVRESE 150
ARLQVSRLYS MTEDEGVRDM LKFLLARETQ HQLQFMKAQE ELEEKYGIIV 200
PGDMKEIEHS EFSHVLMNFS DGDGSKAFEG QVAKDGEKFT YQENPEAMGG 250
IPHIKPGDPR LHNHQG 266
Length:266
Mass (Da):29,743
Last modified:February 16, 2004 - v1
Checksum:i2943A15DD7A60CB1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87070 Genomic DNA. Translation: BAA13239.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87070 Genomic DNA. Translation: BAA13239.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JKU X-ray 1.84 A/B/C/D/E/F 1-266 [» ]
1JKV X-ray 1.39 A/B/C/D/E/F 1-266 [» ]
1O9I X-ray 1.33 A/B/C/D/E/F 1-266 [» ]
ProteinModelPortali P60355.
SMRi P60355. Positions 1-266.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

PeroxiBasei 3978. LplMnCat01.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P60355.

Family and domain databases

Gene3Di 1.20.1260.10. 1 hit.
3.30.1530.10. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR007760. Mn_catalase.
IPR027407. Mn_catalase_dom2.
[Graphical view ]
Pfami PF05067. Mn_catalase. 1 hit.
[Graphical view ]
SUPFAMi SSF47240. SSF47240. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of manganese catalase from Lactobacillus plantarum."
    Igarashi T., Kono Y., Tanaka K.
    J. Biol. Chem. 271:29521-29524(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-32 AND 67-86.
    Strain: ATCC 14431 / T-1043-5.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS), SUBUNIT.
  3. "Outer sphere mutagenesis of Lactobacillus plantarum manganese catalase disrupts the cluster core. Mechanistic implications."
    Whittaker M.M., Barynin V.V., Igarashi T., Whittaker J.W.
    Eur. J. Biochem. 270:1102-1116(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.33 ANGSTROMS), MUTAGENESIS OF TYR-42.

Entry informationi

Entry nameiMCAT_LACPN
AccessioniPrimary (citable) accession number: P60355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: December 11, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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