Reviewed, UniProtKB/Swiss-Prot P60336 (BETA_STAAM)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	SAV2612

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Choline dehydrogenase HAMAP MF_00750		PRO_0000205596
Regions
	Nucleotide binding	9 – 38	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60336-1 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: A6E35293AADDDFA6	FASTA	569	63,610
	10 20 30 40 50 60 MSNKNKSYDY VIIGGGSAGS VLGNRLSEDK DKEVLVLEAG RSDYFWDLFI QMPAALMFPS 70 80 90 100 110 120 GNKFYDWIYS TDEEPHMGGR KVAHARGKVL GGSSSINGMI YQRGNPMDYE GWAEPEGMET 130 140 150 160 170 180 WDFAHCLPYF KKLEKTYGAA PYDKFRGHDG PIKLKRGPAT NPLFQSFFDA GVEAGYHKTP 190 200 210 220 230 240 DVNGFRQEGF GPFDSQVHRG RRMSASRAYL HPAMKRKNLT VETRAFVTEI HYEGRRATGV 250 260 270 280 290 300 TYKKNGKLHT IDANEVILSG GAFNTPQLLQ LSGIGDSEFL KSKGIEPRVH LPGVGENFED 310 320 330 340 350 360 HLEVYIQHKC KEPVSLQPSL DIKRMPFIGL QWIFTRTGAA ASNHFEGGGF VRSNNEVDYP 370 380 390 400 410 420 NLMFHFLPIA VRYDGQKAAV AHGYQVHVGP MYSNSRGSLK IKSKDPFEKP SIRFNYLSTE 430 440 450 460 470 480 EDKKEWVEAI RVARNILSQK AMDPFNGGEI SPGPEVQTDE EILDWVRRDG ETALHPSCSA 490 500 510 520 530 540 KMGPASDPMA VVDPLTMKVH GMENLRVVDA SAMPRTTNGN IHAPVLMLAE KAADIIRGRK 550 560 PLEPQYIDYY KHGVHDENEG AIEVKPYAK

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB58774.1.
RefSeq	NP_373136.1.
3D structure databases
SMR	P60336. Positions 5-536.
ModBase	Search...
Protein-protein interaction databases
STRING	P60336.
2-D gel databases
World-2DPAGE	0002:P60336.
Genome annotation databases
GeneID	1122637.
GenomeReviews	Gene locus SAV2612 in contig BA000017_GR.
KEGG	sav:SAV2612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2303.
HOGENOM	HBG734713.
OMA	RTVTPHG.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV2612-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_STAAM

Accession

Primary (citable) accession number: P60336
Secondary accession number(s): Q99R25

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)