Reviewed, UniProtKB/Swiss-Prot P60335 (PCBP1_MOUSE)

Names and origin

Protein names

Recommended name:
    Poly(rC)-binding protein 1
Alternative name(s):
    Alpha-CP1
    Heterogeneous nuclear ribonucleoprotein E1
      Short name=hnRNP-E1

Gene names

Name:

Pcbp1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Single-stranded nucleic acid binding protein that binds preferentially to oligo dC By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 3 KH domains.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding RNA-binding
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	mRNA processing Ref.1 Traceable author statement. Source: MGI
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Ref.1 Traceable author statement. Source: MGI protein binding Inferred from physical interaction. Source: IntAct translation activator activity Ref.1 Traceable author statement. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Hdac9	Q99N13	2	EBI-309059,EBI-645361

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Poly(rC)-binding protein 1		PRO_0000050088
Regions
	Domain	13 – 75	63	KH 1
	Domain	97 – 162	66	KH 2
	Domain	279 – 343	65	KH 3
Amino acid modifications
	Modified residue	173	1	Phosphoserine Ref.4
	Modified residue	189	1	Phosphoserine By similarity
	Modified residue	190	1	Phosphoserine By similarity
	Modified residue	246	1	Phosphoserine By similarity
	Modified residue	262	1	Phosphoserine By similarity
	Modified residue	263	1	Phosphoserine By similarity
	Modified residue	264	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60335-1 [UniParc]. Last modified February 16, 2004. Version 1. Checksum: 6D1A261276CA206D	FASTA	356	37,498
	10 20 30 40 50 60 MDAGVTESGL NVTLTIRLLM HGKEVGSIIG KKGESVKRIR EESGARINIS EGNCPERIIT 70 80 90 100 110 120 LTGPTNAIFK AFAMIIDKLE EDINSSMTNS TAASRPPVTL RLVVPATQCG SLIGKGGCKI 130 140 150 160 170 180 KEIRESTGAQ VQVAGDMLPN STERAITIAG VPQSVTECVK QICLVMLETL SQSPQGRVMT 190 200 210 220 230 240 IPYQPMPASS PVICAGGQDR CSDAAGYPHA THDLEGPPLD AYSIQGQHTI SPLDLAKLNQ 250 260 270 280 290 300 VARQQSHFAM MHGGTGFAGI DSSSPEVKGY WASLDASTQT THELTIPNNL IGCIIGRQGA 310 320 330 340 350 NINEIRQMSG AQIKIANPVE GSSGRQVTIT GSAASISLAQ YLINARLSSE KGMGCS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog." Makeyev A.V., Chkheidze A.N., Liebhaber S.A. J. Biol. Chem. 274:24849-24857(1999) [PubMed: 10455157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Mammary gland.
[3]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 326-346, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[4]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF139894 mRNA. Translation: AAD51920.1. AF139895 Genomic DNA. Translation: AAD51921.1. BC069915 mRNA. Translation: AAH69915.1. BC004793 mRNA. Translation: AAH04793.1.
IPI	IPI00128904.
RefSeq	NP_035995.1.
UniGene	Mm.274146
3D structure databases
SMR	P60335. Positions 11-169, 102-348.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60335. 6 interactions.
STRING	P60335.
PTM databases
PhosphoSite	P60335.
2-D gel databases
REPRODUCTION-2DPAGE	P60335.
Proteomic databases
PRIDE	P60335.
Genome annotation databases
Ensembl	ENSMUST00000053015; ENSMUSP00000054863; ENSMUSG00000051695; Mus musculus. [Genome view]
GeneID	23983.
KEGG	mmu:23983.
UCSC	uc009csc.1. mouse.
Organism-specific databases
CTD	23983.
MGI	MGI:1345635. Pcbp1.
Phylogenomic databases
eggNOG	roNOG07937.
HOGENOM	HBG445439.
HOVERGEN	P60335.
InParanoid	P60335.
OMA	NISERNC.
PhylomeDB	P60335.
Gene expression databases
ArrayExpress	P60335.
Bgee	P60335.
CleanEx	MM_PCBP1.
Genevestigator	P60335.
GermOnline	ENSMUSG00000051695. Mus musculus.
Family and domain databases
InterPro	IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. [Graphical view]
Pfam	PF00013. KH_1. 3 hits. [Graphical view]
SMART	SM00322. KH. 3 hits. [Graphical view]
PROSITE	PS50084. KH_TYPE_1. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	303869.
SOURCE	Search...

Entry information

Entry name

PCBP1_MOUSE

Accession

Primary (citable) accession number: P60335

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)