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Protein

Cysteine dioxygenase type 1

Gene

Cdo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cysteine + O2 = 3-sulfinoalanine.

Cofactori

Fe2+, Ni2+, Zn2+Note: Binds 1 Fe2+ cation per subunit. Ni2+ and Zn2+ can be used to a lesser extent.

Pathwayi: taurine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hypotaurine from L-cysteine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cysteine dioxygenase type 1 (Cdo1)
  2. Cysteine sulfinic acid decarboxylase (Csad)
This subpathway is part of the pathway taurine biosynthesis, which is itself part of Organosulfur biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hypotaurine from L-cysteine, the pathway taurine biosynthesis and in Organosulfur biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi86Iron; via tele nitrogen; catalytic1 Publication1
Metal bindingi88Iron; via tele nitrogen; catalytic1 Publication1
Metal bindingi140Iron; via tele nitrogen; catalytic1 Publication1

GO - Molecular functioni

  • cysteine dioxygenase activity Source: MGI
  • ferrous iron binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.20. 3474.
ReactomeiR-MMU-1614558. Degradation of cysteine and homocysteine.
R-MMU-375170. CDO in myogenesis.
UniPathwayiUPA00012; UER00537.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine dioxygenase type 1 (EC:1.13.11.20)
Alternative name(s):
Cysteine dioxygenase type I
Short name:
CDO
Short name:
CDO-I
Gene namesi
Name:Cdo1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:105925. Cdo1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002066071 – 200Cysteine dioxygenase type 1Add BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki93 ↔ 1573'-(S-cysteinyl)-tyrosine (Cys-Tyr)1 Publication

Post-translational modificationi

The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.1 Publication

Keywords - PTMi

Thioether bond

Proteomic databases

MaxQBiP60334.
PaxDbiP60334.
PeptideAtlasiP60334.
PRIDEiP60334.

2D gel databases

REPRODUCTION-2DPAGEP60334.

PTM databases

iPTMnetiP60334.
PhosphoSitePlusiP60334.
SwissPalmiP60334.

Expressioni

Tissue specificityi

Highest expression in liver. Also expressed in kidney, lung, brain and small intestine.1 Publication

Gene expression databases

BgeeiENSMUSG00000033022.
CleanExiMM_CDO1.
GenevisibleiP60334. MM.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiP60334. 1 interactor.
STRINGi10090.ENSMUSP00000046517.

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 22Combined sources11
Beta strandi24 – 27Combined sources4
Helixi30 – 39Combined sources10
Helixi44 – 47Combined sources4
Helixi48 – 50Combined sources3
Beta strandi55 – 57Combined sources3
Beta strandi59 – 64Combined sources6
Helixi66 – 68Combined sources3
Beta strandi71 – 77Combined sources7
Beta strandi92 – 100Combined sources9
Beta strandi102 – 107Combined sources6
Beta strandi112 – 114Combined sources3
Beta strandi121 – 125Combined sources5
Beta strandi130 – 133Combined sources4
Turni135 – 137Combined sources3
Beta strandi139 – 143Combined sources5
Beta strandi151 – 159Combined sources9
Beta strandi162 – 167Combined sources6
Turni169 – 171Combined sources3
Beta strandi174 – 178Combined sources5
Beta strandi182 – 184Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ATFX-ray1.75A2-200[»]
2Q4SX-ray1.75A2-200[»]
ProteinModelPortaliP60334.
SMRiP60334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60334.

Family & Domainsi

Sequence similaritiesi

Belongs to the cysteine dioxygenase family.Curated

Phylogenomic databases

eggNOGiKOG4064. Eukaryota.
ENOG410XQM4. LUCA.
GeneTreeiENSGT00390000018226.
HOGENOMiHOG000177818.
HOVERGENiHBG004469.
InParanoidiP60334.
KOiK00456.
OMAiDASRGYT.
OrthoDBiEOG091G0LAR.
PhylomeDBiP60334.
TreeFamiTF105636.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. CDO_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P60334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY
60 70 80 90 100
AKFDQYRYTR NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG
110 120 130 140 150
NLKETLFDWP DKKSNEMIKK SERTLRENQC AYINDSIGLH RVENVSHTEP
160 170 180 190 200
AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT FHSKFGIRTP FTTSGSLENN
Length:200
Mass (Da):23,026
Last modified:February 16, 2004 - v1
Checksum:i118F1A3326B340F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF355472
, AF355469, AF355470, AF355471 Genomic DNA. Translation: AAK53364.1.
AK004249 mRNA. Translation: BAB23236.1.
AK149582 mRNA. Translation: BAE28973.1.
BC013638 mRNA. Translation: AAH13638.1.
CCDSiCCDS29234.1.
RefSeqiNP_149026.1. NM_033037.3.
UniGeneiMm.241056.

Genome annotation databases

EnsembliENSMUST00000035804; ENSMUSP00000046517; ENSMUSG00000033022.
GeneIDi12583.
KEGGimmu:12583.
UCSCiuc008evt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF355472
, AF355469, AF355470, AF355471 Genomic DNA. Translation: AAK53364.1.
AK004249 mRNA. Translation: BAB23236.1.
AK149582 mRNA. Translation: BAE28973.1.
BC013638 mRNA. Translation: AAH13638.1.
CCDSiCCDS29234.1.
RefSeqiNP_149026.1. NM_033037.3.
UniGeneiMm.241056.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ATFX-ray1.75A2-200[»]
2Q4SX-ray1.75A2-200[»]
ProteinModelPortaliP60334.
SMRiP60334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP60334. 1 interactor.
STRINGi10090.ENSMUSP00000046517.

PTM databases

iPTMnetiP60334.
PhosphoSitePlusiP60334.
SwissPalmiP60334.

2D gel databases

REPRODUCTION-2DPAGEP60334.

Proteomic databases

MaxQBiP60334.
PaxDbiP60334.
PeptideAtlasiP60334.
PRIDEiP60334.

Protocols and materials databases

DNASUi12583.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035804; ENSMUSP00000046517; ENSMUSG00000033022.
GeneIDi12583.
KEGGimmu:12583.
UCSCiuc008evt.1. mouse.

Organism-specific databases

CTDi1036.
MGIiMGI:105925. Cdo1.

Phylogenomic databases

eggNOGiKOG4064. Eukaryota.
ENOG410XQM4. LUCA.
GeneTreeiENSGT00390000018226.
HOGENOMiHOG000177818.
HOVERGENiHBG004469.
InParanoidiP60334.
KOiK00456.
OMAiDASRGYT.
OrthoDBiEOG091G0LAR.
PhylomeDBiP60334.
TreeFamiTF105636.

Enzyme and pathway databases

UniPathwayiUPA00012; UER00537.
BRENDAi1.13.11.20. 3474.
ReactomeiR-MMU-1614558. Degradation of cysteine and homocysteine.
R-MMU-375170. CDO in myogenesis.

Miscellaneous databases

EvolutionaryTraceiP60334.
PROiP60334.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033022.
CleanExiMM_CDO1.
GenevisibleiP60334. MM.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. CDO_1.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCDO1_MOUSE
AccessioniPrimary (citable) accession number: P60334
Secondary accession number(s): Q3UED8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.