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P60330 (ESPL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
July 9, 2014.
Version 83.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Separin EC=3.4.22.49 Alternative name(s): Caspase-like protein ESPL1 Extra spindle poles-like 1 protein Separase | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 2118 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms By similarity. |
| Catalytic activity | All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage. |
| Enzyme regulation | Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1121 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate By similarity. |
| Subunit structure | Interacts with PTTG1. Interacts with RAD21 By similarity. |
| Subcellular location | |
| Post-translational modification | Autocleaves. This function, which is not essential for its protease activity, is unknown By similarity. Phosphorylated by CDK1. There is 8 Ser/Thr phosphorylation sites. Among them, only Ser-1121 phosphorylation is the major site, which conducts to the enzyme inactivation By similarity. |
| Sequence similarities | Contains 1 peptidase C50 domain. |
| Sequence caution | The sequence BAC97882.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 2118 | 2118 | Separin | PRO_0000205901 | |||||
Regions | |||||||||
| Domain | 1941 – 2036 | 96 | Peptidase C50 | ||||||
Sites | |||||||||
| Active site | 2025 | 1 | By similarity | ||||||
| Site | 1502 – 1503 | 2 | Cleavage; by autolysis By similarity | ||||||
| Site | 1531 – 1532 | 2 | Cleavage; by autolysis By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1121 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1391 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1394 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1504 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryonic tail. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [3] | Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1241-1260, IDENTIFICATION BY MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus. |
| [4] | "The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK129072 mRNA. Translation: BAC97882.1. Different initiation. BC145846 mRNA. Translation: AAI45847.1. |
| CCDS | CCDS27878.1. |
| RefSeq | NP_001014976.1. NM_001014976.2. XP_006520320.1. XM_006520257.1. XP_006520321.1. XM_006520258.1. XP_006520322.1. XM_006520259.1. |
| UniGene | Mm.288324. |
3D structure databases | |
| ModBase | Search... |
| MobiDB | Search... |
Protein-protein interaction databases | |
| BioGrid | 222968. 5 interactions. |
| IntAct | P60330. 5 interactions. |
| STRING | 10090.ENSMUSP00000064465. |
Protein family/group databases | |
| MEROPS | C50.002. |
PTM databases | |
| PhosphoSite | P60330. |
Proteomic databases | |
| PaxDb | P60330. |
| PRIDE | P60330. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000064924; ENSMUSP00000064465; ENSMUSG00000058290. |
| GeneID | 105988. |
| KEGG | mmu:105988. |
| UCSC | uc007xvf.2. mouse. |
Organism-specific databases | |
| CTD | 9700. |
| MGI | MGI:2146156. Espl1. |
| Rouge | Search... |
Phylogenomic databases | |
| eggNOG | COG5155. |
| GeneTree | ENSGT00390000004990. |
| HOGENOM | HOG000290657. |
| HOVERGEN | HBG051510. |
| InParanoid | A6H6E3. |
| KO | K02365. |
| OMA | ELAQVLC. |
| OrthoDB | EOG7PCJGQ. |
| PhylomeDB | P60330. |
| TreeFam | TF101169. |
Gene expression databases | |
| Bgee | P60330. |
| CleanEx | MM_ESPL1. |
| Genevestigator | P60330. |
Family and domain databases | |
| InterPro | IPR005314. Peptidase_C50. [Graphical view] |
| PANTHER | PTHR12792. PTHR12792. 1 hit. |
| Pfam | PF03568. Peptidase_C50. 1 hit. [Graphical view] |
| PROSITE | PS51700. SEPARIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 357998. |
| PRO | P60330. |
| SOURCE | Search... |
Entry information
| Entry name | ESPL1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P60330 Secondary accession number(s): A6H6E3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Peptidase families Classification of peptidase families and list of entries |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |