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P60330

- ESPL1_MOUSE

UniProt

P60330 - ESPL1_MOUSE

Protein

Separin

Gene

Espl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (02 Feb 2004)
      Previous versions | rss
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    Functioni

    Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms By similarity.By similarity

    Catalytic activityi

    All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.

    Enzyme regulationi

    Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1121 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1502 – 15032Cleavage; by autolysisBy similarity
    Sitei1531 – 15322Cleavage; by autolysisBy similarity
    Active sitei2025 – 20251By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: MGI
    2. peptidase activity Source: MGI

    GO - Biological processi

    1. chromosome segregation Source: MGI
    2. homologous chromosome segregation Source: MGI
    3. meiosis I Source: MGI
    4. meiotic spindle organization Source: MGI
    5. mitotic nuclear division Source: MGI
    6. mitotic sister chromatid segregation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Chromosome partition

    Enzyme and pathway databases

    ReactomeiREACT_207679. Separation of Sister Chromatids.

    Protein family/group databases

    MEROPSiC50.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Separin (EC:3.4.22.49)
    Alternative name(s):
    Caspase-like protein ESPL1
    Extra spindle poles-like 1 protein
    Separase
    Gene namesi
    Name:Espl1
    Synonyms:Esp1, Kiaa0165
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:2146156. Espl1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21182118SeparinPRO_0000205901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1121 – 11211PhosphoserineBy similarity
    Modified residuei1391 – 13911PhosphoserineBy similarity
    Modified residuei1394 – 13941PhosphoserineBy similarity
    Modified residuei1504 – 15041PhosphoserineBy similarity

    Post-translational modificationi

    Autocleaves. This function, which is not essential for its protease activity, is unknown By similarity.By similarity
    Phosphorylated by CDK1. There is 8 Ser/Thr phosphorylation sites. Among them, only Ser-1121 phosphorylation is the major site, which conducts to the enzyme inactivation By similarity.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Phosphoprotein

    Proteomic databases

    PaxDbiP60330.
    PRIDEiP60330.

    PTM databases

    PhosphoSiteiP60330.

    Expressioni

    Gene expression databases

    BgeeiP60330.
    CleanExiMM_ESPL1.
    GenevestigatoriP60330.

    Interactioni

    Subunit structurei

    Interacts with PTTG1. Interacts with RAD21 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi222968. 5 interactions.
    IntActiP60330. 5 interactions.
    STRINGi10090.ENSMUSP00000064465.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1941 – 203696Peptidase C50Add
    BLAST

    Sequence similaritiesi

    Contains 1 peptidase C50 domain.Curated

    Phylogenomic databases

    eggNOGiCOG5155.
    GeneTreeiENSGT00390000004990.
    HOGENOMiHOG000290657.
    HOVERGENiHBG051510.
    InParanoidiA6H6E3.
    KOiK02365.
    OMAiELAQVLC.
    OrthoDBiEOG7PCJGQ.
    PhylomeDBiP60330.
    TreeFamiTF101169.

    Family and domain databases

    InterProiIPR005314. Peptidase_C50.
    [Graphical view]
    PANTHERiPTHR12792. PTHR12792. 1 hit.
    PfamiPF03568. Peptidase_C50. 1 hit.
    [Graphical view]
    PROSITEiPS51700. SEPARIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60330-1 [UniParc]FASTAAdd to Basket

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    MRNFKGVNFA TLLCSKEETQ QLLPDLKEFL SRSRTDFPSS RTDAERRQIC     50
    DTILRACTQQ LTAKLDCPGH LRSILDLAEL ACDGYLLSTP QRPPLYLERI 100
    LFILLRNGST QGSPDTVLRL AQPLHACLVQ NSGEAAPQDY EAVTRGSFSL 150
    FWKGAEALLE RRAAFSTRLN ALSFLVLLED GSVPCEVPHF ASPTACRLVA 200
    AYQLYDATGQ GLDEADADFL YEVLSRHLIR VLVGEGGSSP GPLSPQRALC 250
    LLEITLEHCR RLCWNHHHRQ AARAVERARN HLEKTSVAPS LQLCQMGVEL 300
    LEAVEERPGA VAQLLRKAAA VLINSIEAPS PPLRALYDSC QFFLSGLERG 350
    IRRHCGLDAI LSLFAFLGGY SSLVRHLREV SEASSKQQQC LLQMHFQGFH 400
    LFTGIVYDFA QGCQATELAQ LVDGCRSAAV WMLEALEGLS GGELADYLSM 450
    TASYTSNLAY SFFSQKLYEE ACVISEPVCQ HLGSATSGAC PEVPPEKLHR 500
    CFRLHVESLK KLGKQAQGCK MVTLWLAALK PYSLEHMVEP VTFWVRVKMD 550
    ASRAGDKELQ LQTLRDSLSC WDPETQSLLL REELRAYKSV RADTGQERFN 600
    IICDLLELSP EETAAGAWAR ATYLVELAQV LCYHNFTQQT NCSALDAVQE 650
    ALQLLESVSP EAQEQDRLLD DKAQALLWLY ICTLEAKMQE GIERDRRAQA 700
    PSNLEEFEVN DLNYEDKLQE DRFLYSSIAF NLAADAAQSK CLDQALTLWK 750
    EVLTKGRAPA VRCLQQTAAS LQILAAVYQL VAKPLQALET LLLLQIVSKR 800
    LQDHAKAASS SCQLTQLLLN LGCPSYAQLY LEEAESSLRS LDQTSDACQL 850
    LSLTCALLGS QLCWACQKVT AGVSLLLSVL RDPALQKSSK AWYLLRVQAL 900
    QVLAFYLSLS SNLLSSALRE QLWDQGWQTP ETALIDAHKL LRSIIILLMG 950
    SDVLSIQKAA TESPFLDYGE NLVQKWQVLT EVLTCSERLV GRLGRLGNVS 1000
    EAKAFCLEAL KLTTKLQIPR QCALFLVLKG ELELARGDID LCQSDLQQVL 1050
    FLLESSTEFG VVTQHPDSVK KVHTQKGKHK AQGPCFPPLS EEEPFLKGPA 1100
    LELVDTVLNE PGPIQSSVNS SPVLKTKPPP NPGFLSHLPS CDCLLCASPA 1150
    LSAVCLRWVL VTAGVRLATG HKAQGLDLLQ AVLTRCPAAT KRFTQSLQAS 1200
    LNHRTTPSCV PSLFDEIMAQ VYTHLALEFL NQTSEKSLGK VLASGLKFVA 1250
    TRIQSLEIWR AHLLLVQALA KLAHFSCCTS ELFASSWGWH PPLVKSLPVL 1300
    EPAKIRRQKC SGRGRRRIAS VPPPLHNSSQ KGLEEEGPPC TPKPPGRARQ 1350
    AGPRVPFTIF EEVHPTKSKL QVPLAPRVHR RAQTRLKVIF SDDSDLEDLV 1400
    SADTQLVEEP KRRGTASRTR GQTRKGRSLK TDAVVAIEST PGHSSVSGRT 1450
    RRARKVASRN CEEESPKAPL CVWASQGPEI MRSIPEEEPV DNHLEKSFEI 1500
    LRGSDGEDSA SGEKAAAADT GLPVGECEVL RRDSSKAERP VLYSDTEANS 1550
    DPSPWLPPFS VPAPIDLSTL DSISDSLSIA FRGVSHCPPS GLYAHLCRFL 1600
    ALCLGHRDPY ATAFLVAESI SITCRHQLLT HLHRQLSKAQ KQQESPELAE 1650
    HLQRLDLKER PGGVPLARIQ RLFSFKALGS GCFPQAEKES FQERLALIPS 1700
    GVTVCVLALA TLQPGTLSNT LLLTRLEKDN PPITVKIPTA QNKLPLSAVL 1750
    KEFDAIQKDQ KENSSCTEKR VWWTGRLALD QRMEALITAL EEQVLGCWRG 1800
    LLLPCSADPS LAQEASKLQE LLRECGWEYP DSTLLKVILS GARILTSQDV 1850
    QALACGLCPA QPDRAQVLLS EAVGQVQSQE APRSQHLVLV LDKDLQKLPW 1900
    ESTPILQAQP VTRLPSFRFL LSYTVTKEAG ASSVLSQGVD PQNTFYVLNP 1950
    HSNLSSTEER FRASFSSETG WKGVIGEVPS LDQVQAALTE RDLYIYAGHG 2000
    AGARFLDGQA VLRLSCRAVA LLFGCSSAAL AVHGNLEGAG IVLKYIMAGC 2050
    PLFLGNLWDV TDRDIDRYTE ALLQGWLGAG PGAPFLYYAS QARQAPRLKY 2100
    LIGAAPVAYG LPISLQTP 2118
    Length:2,118
    Mass (Da):233,035
    Last modified:February 2, 2004 - v1
    Checksum:i484FCE178C0984B6
    GO

    Sequence cautioni

    The sequence BAC97882.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129072 mRNA. Translation: BAC97882.1. Different initiation.
    BC145846 mRNA. Translation: AAI45847.1.
    CCDSiCCDS27878.1.
    RefSeqiNP_001014976.1. NM_001014976.2.
    XP_006520320.1. XM_006520257.1.
    XP_006520321.1. XM_006520258.1.
    XP_006520322.1. XM_006520259.1.
    UniGeneiMm.288324.

    Genome annotation databases

    EnsembliENSMUST00000064924; ENSMUSP00000064465; ENSMUSG00000058290.
    GeneIDi105988.
    KEGGimmu:105988.
    UCSCiuc007xvf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129072 mRNA. Translation: BAC97882.1 . Different initiation.
    BC145846 mRNA. Translation: AAI45847.1 .
    CCDSi CCDS27878.1.
    RefSeqi NP_001014976.1. NM_001014976.2.
    XP_006520320.1. XM_006520257.1.
    XP_006520321.1. XM_006520258.1.
    XP_006520322.1. XM_006520259.1.
    UniGenei Mm.288324.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 222968. 5 interactions.
    IntActi P60330. 5 interactions.
    STRINGi 10090.ENSMUSP00000064465.

    Protein family/group databases

    MEROPSi C50.002.

    PTM databases

    PhosphoSitei P60330.

    Proteomic databases

    PaxDbi P60330.
    PRIDEi P60330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064924 ; ENSMUSP00000064465 ; ENSMUSG00000058290 .
    GeneIDi 105988.
    KEGGi mmu:105988.
    UCSCi uc007xvf.2. mouse.

    Organism-specific databases

    CTDi 9700.
    MGIi MGI:2146156. Espl1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5155.
    GeneTreei ENSGT00390000004990.
    HOGENOMi HOG000290657.
    HOVERGENi HBG051510.
    InParanoidi A6H6E3.
    KOi K02365.
    OMAi ELAQVLC.
    OrthoDBi EOG7PCJGQ.
    PhylomeDBi P60330.
    TreeFami TF101169.

    Enzyme and pathway databases

    Reactomei REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 357998.
    PROi P60330.
    SOURCEi Search...

    Gene expression databases

    Bgeei P60330.
    CleanExi MM_ESPL1.
    Genevestigatori P60330.

    Family and domain databases

    InterProi IPR005314. Peptidase_C50.
    [Graphical view ]
    PANTHERi PTHR12792. PTHR12792. 1 hit.
    Pfami PF03568. Peptidase_C50. 1 hit.
    [Graphical view ]
    PROSITEi PS51700. SEPARIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryonic tail.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1241-1260, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiESPL1_MOUSE
    AccessioniPrimary (citable) accession number: P60330
    Secondary accession number(s): A6H6E3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: February 2, 2004
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3