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Protein

Nanos homolog 3

Gene

NANOS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri57 – 11155Nanos-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Oogenesis, Spermatogenesis, Translation regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nanos homolog 3
Short name:
NOS-3
Gene namesi
Name:NANOS3
Synonyms:NOS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:22048. NANOS3.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Cytoplasmic granule By similarity
  • CytoplasmP-body By similarity

  • Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic mRNA processing body Source: UniProtKB
  • cytoplasmic stress granule Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134867615.

Polymorphism and mutation databases

BioMutaiNANOS3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 173173Nanos homolog 3PRO_0000207689Add
BLAST

Proteomic databases

PRIDEiP60323.

PTM databases

iPTMnetiP60323.
PhosphoSiteiP60323.
SwissPalmiP60323.

Expressioni

Tissue specificityi

Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.1 Publication

Developmental stagei

Fetal ovary and fetal testis (at protein level).1 Publication

Gene expression databases

BgeeiP60323.
CleanExiHS_NANOS3.
HS_NOS3.
GenevisibleiP60323. HS.

Organism-specific databases

HPAiHPA062989.

Interactioni

Subunit structurei

Binds mRNA from germ cells. Interacts with PUM2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi131219. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP60323.
SMRiP60323. Positions 56-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 8528C2HC 1Add
BLAST
Motifi93 – 10917C2HC 2Add
BLAST

Domaini

The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the nanos family.PROSITE-ProRule annotation
Contains 1 nanos-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri57 – 11155Nanos-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00640000091383.
HOGENOMiHOG000082528.
HOVERGENiHBG052575.
InParanoidiP60323.
KOiK18761.
OMAiYSHTTRN.
PhylomeDBiP60323.
TreeFamiTF326882.

Family and domain databases

InterProiIPR008705. Nanos/Xcar2.
IPR024161. Znf_nanos-typ.
[Graphical view]
PANTHERiPTHR12887. PTHR12887. 1 hit.
PfamiPF05741. zf-nanos. 1 hit.
[Graphical view]
PROSITEiPS51522. ZF_NANOS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60323-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTFDLWTDY LGLAHLVRAL SGKEGPETRL SPQPEPEPML EPDQKRSLES
60 70 80 90 100
SPAPERLCSF CKHNGESRAI YQSHVLKDEA GRVLCPILRD YVCPQCGATR
110 120 130 140 150
ERAHTRRFCP LTGQGYTSVY SHTTRNSAGK KLVRPDKAKT QDTGHRRGGG
160 170
GGAGFRGAGK SEPSPSCSPS MST
Length:173
Mass (Da):18,844
Last modified:February 2, 2004 - v1
Checksum:i14C18866070795D9
GO
Isoform 2 (identifier: P60323-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-42: P → PVSALEPMPAPESVPVPGPK

Show »
Length:192
Mass (Da):20,728
Checksum:i9702678D89D57EAF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei42 – 421P → PVSALEPMPAPESVPVPGPK in isoform 2. 1 PublicationVSP_038695

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020916 Genomic DNA. No translation available.
BC101209 mRNA. Translation: AAI01210.1.
BC101210 mRNA. Translation: AAI01211.1.
BM702754 mRNA. No translation available.
CCDSiCCDS42511.1. [P60323-2]
RefSeqiNP_001092092.1. NM_001098622.2. [P60323-2]
UniGeneiHs.127982.

Genome annotation databases

EnsembliENST00000339133; ENSP00000341992; ENSG00000187556. [P60323-2]
ENST00000397555; ENSP00000380687; ENSG00000187556. [P60323-1]
GeneIDi342977.
KEGGihsa:342977.
UCSCiuc002mxj.5. human. [P60323-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC020916 Genomic DNA. No translation available.
BC101209 mRNA. Translation: AAI01210.1.
BC101210 mRNA. Translation: AAI01211.1.
BM702754 mRNA. No translation available.
CCDSiCCDS42511.1. [P60323-2]
RefSeqiNP_001092092.1. NM_001098622.2. [P60323-2]
UniGeneiHs.127982.

3D structure databases

ProteinModelPortaliP60323.
SMRiP60323. Positions 56-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131219. 4 interactions.

PTM databases

iPTMnetiP60323.
PhosphoSiteiP60323.
SwissPalmiP60323.

Polymorphism and mutation databases

BioMutaiNANOS3.

Proteomic databases

PRIDEiP60323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339133; ENSP00000341992; ENSG00000187556. [P60323-2]
ENST00000397555; ENSP00000380687; ENSG00000187556. [P60323-1]
GeneIDi342977.
KEGGihsa:342977.
UCSCiuc002mxj.5. human. [P60323-1]

Organism-specific databases

CTDi342977.
GeneCardsiNANOS3.
HGNCiHGNC:22048. NANOS3.
HPAiHPA062989.
MIMi608229. gene.
neXtProtiNX_P60323.
PharmGKBiPA134867615.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00640000091383.
HOGENOMiHOG000082528.
HOVERGENiHBG052575.
InParanoidiP60323.
KOiK18761.
OMAiYSHTTRN.
PhylomeDBiP60323.
TreeFamiTF326882.

Miscellaneous databases

GenomeRNAii342977.
PROiP60323.
SOURCEiSearch...

Gene expression databases

BgeeiP60323.
CleanExiHS_NANOS3.
HS_NOS3.
GenevisibleiP60323. HS.

Family and domain databases

InterProiIPR008705. Nanos/Xcar2.
IPR024161. Znf_nanos-typ.
[Graphical view]
PANTHERiPTHR12887. PTHR12887. 1 hit.
PfamiPF05741. zf-nanos. 1 hit.
[Graphical view]
PROSITEiPS51522. ZF_NANOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "Normalization and subtraction: two approaches to facilitate gene discovery."
    Bonaldo M.F., Lennon G., Soares M.B.
    Genome Res. 6:791-806(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 122-173 (ISOFORMS 1/2).
  4. "NANOS3 function in human germ cell development."
    Julaton V.T., Reijo Pera R.A.
    Hum. Mol. Genet. 20:2238-2250(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiNANO3_HUMAN
AccessioniPrimary (citable) accession number: P60323
Secondary accession number(s): Q495E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.