P60323 (NANO3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nanos homolog 3
Short name=NOS-3

Gene names

Name:	NANOS3
Synonyms:	NOS3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis. Ref.4
Subunit structure	Binds mRNA from germ cells. Interacts with PUM2 By similarity.
Subcellular location	Nucleus. Cytoplasm. Cytoplasmic granule By similarity. Cytoplasm › P-body By similarity. Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body By similarity. Ref.4
Tissue specificity	Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids. Ref.4
Developmental stage	Fetal ovary and fetal testis (at protein level). Ref.4
Domain	The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein By similarity.
Sequence similarities	Belongs to the nanos family. Contains 1 nanos-type zinc finger.

Ontologies

Keywords
Biological process	Differentiation Oogenesis Spermatogenesis Translation regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB germ cell development Inferred from mutant phenotype Ref.4. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell cycle Inferred from sequence or structural similarity. Source: UniProtKB regulation of translation Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasmic mRNA processing body Inferred from sequence or structural similarity. Source: UniProtKB cytoplasmic stress granule Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from direct assay Ref.4. Source: UniProtKB
Molecular function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P60323-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P60323-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-42: P → PVSALEPMPAPESVPVPGPK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Nanos homolog 3		PRO_0000207689
Regions
	Zinc finger	57 – 111	55	Nanos-type
	Motif	58 – 85	28	C2HC 1
	Motif	93 – 109	17	C2HC 2
Natural variations
	Alternative sequence	42	1	P → PVSALEPMPAPESVPVPGPK in isoform 2.		VSP_038695

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 2, 2004. Version 1. Checksum: 14C18866070795D9	FASTA	173	18,844
	10 20 30 40 50 60 MGTFDLWTDY LGLAHLVRAL SGKEGPETRL SPQPEPEPML EPDQKRSLES SPAPERLCSF 70 80 90 100 110 120 CKHNGESRAI YQSHVLKDEA GRVLCPILRD YVCPQCGATR ERAHTRRFCP LTGQGYTSVY 130 140 150 160 170 SHTTRNSAGK KLVRPDKAKT QDTGHRRGGG GGAGFRGAGK SEPSPSCSPS MST
	Isoform 2 [UniParc]. Checksum: 9702678D89D57EAF	FASTA	192	20,728
	10 20 30 40 50 60 MGTFDLWTDY LGLAHLVRAL SGKEGPETRL SPQPEPEPML EPVSALEPMP APESVPVPGP 70 80 90 100 110 120 KDQKRSLESS PAPERLCSFC KHNGESRAIY QSHVLKDEAG RVLCPILRDY VCPQCGATRE 130 140 150 160 170 180 RAHTRRFCPL TGQGYTSVYS HTTRNSAGKK LVRPDKAKTQ DTGHRRGGGG GAGFRGAGKS 190 EPSPSCSPSM ST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"Normalization and subtraction: two approaches to facilitate gene discovery." Bonaldo M.F., Lennon G., Soares M.B. Genome Res. 6:791-806(1996) [PubMed: 8889548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 122-173 (ISOFORMS 1/2).
[4]	"NANOS3 function in human germ cell development." Julaton V.T., Reijo Pera R.A. Hum. Mol. Genet. 20:2238-2250(2011) [PubMed: 21421998] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC020916 Genomic DNA. No translation available. BC101209 mRNA. Translation: AAI01210.1. BC101210 mRNA. Translation: AAI01211.1. BM702754 mRNA. No translation available.
IPI	IPI00397636. IPI00746675.
RefSeq	NP_001092092.1. NM_001098622.2.
UniGene	Hs.127982.
3D structure databases
ProteinModelPortal	P60323.
SMR	P60323. Positions 56-110.
ModBase	Search...
Protein-protein interaction databases
STRING	P60323.
Polymorphism databases
DMDM	41688563.
Proteomic databases
PRIDE	P60323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000397555; ENSP00000380687; ENSG00000187556.
GeneID	342977.
KEGG	hsa:342977.
Organism-specific databases
CTD	342977.
GeneCards	GC19P013988.
HGNC	HGNC:22048. NANOS3.
MIM	608229. gene.
neXtProt	NX_P60323.
PharmGKB	PA134867615.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17510.
GeneTree	ENSGT00390000005985.
HOVERGEN	HBG052575.
OMA	NLWTDYL.
PhylomeDB	P60323.
Gene expression databases
ArrayExpress	P60323.
Bgee	P60323.
CleanEx	HS_NANOS3. HS_NOS3.
Genevestigator	P60323.
GermOnline	ENSG00000187556. Homo sapiens.
Family and domain databases
InterPro	IPR008705. Nanos/Xcar2. IPR024161. Znf_nanos-typ. [Graphical view]
PANTHER	PTHR12887. Znf_Nanos. 1 hit.
Pfam	PF05741. zf-nanos. 1 hit. [Graphical view]
PROSITE	PS51522. ZF_NANOS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

NANO3_HUMAN

Accession

Primary (citable) accession number: P60323
Secondary accession number(s): Q495E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	February 2, 2004
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.