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Protein

Nanos homolog 2

Gene

Nanos2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 114Nanos-typePROSITE-ProRule annotationAdd BLAST55

GO - Molecular functioni

  • mRNA binding Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • germ-line stem cell population maintenance Source: UniProtKB
  • mRNA catabolic process Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of meiotic nuclear division Source: UniProtKB
  • negative regulation of translation Source: MGI
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: MGI
  • spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis, Translation regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nanos homolog 2
Short name:
NOS-2
Gene namesi
Name:Nanos2
Synonyms:Nos2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2676627. Nanos2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic mRNA processing body Source: UniProtKB
  • nucleus Source: MGI
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice show a gradual loss of the germ cell population within a few cycles of spermatogenesis which is caused by the depletion of spermatogonial stem cells that produce differentiating spermatogenic cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076881 – 136Nanos homolog 2Add BLAST136

Proteomic databases

PaxDbiP60322.
PRIDEiP60322.

PTM databases

SwissPalmiP60322.

Expressioni

Tissue specificityi

Predominantly expressed in male germ cells. Expressed in self-renewing spermatogonial stem cells and developing gonads.2 Publications

Developmental stagei

First detectable at E13.5 in the male gonocytes, levels increase until about E16.5 and then slightly decrease by E17.5 (at protein level). Expression is maintained in all male gonocytes during embryogenesis, but becomes confined to a small population of the spermatogonia after birth.2 Publications

Gene expression databases

BgeeiENSMUSG00000051965.
CleanExiMM_NANOS2.
MM_NOS2.
ExpressionAtlasiP60322. baseline and differential.

Interactioni

Subunit structurei

Interacts with CNOT1, CNOT3, CNOT6L, CNOT7 and CNOT9.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Cnot1Q6ZQ083EBI-6507212,EBI-682479

Protein-protein interaction databases

BioGridi237543. 1 interactor.
DIPiDIP-59508N.
IntActiP60322. 7 interactors.
STRINGi10090.ENSMUSP00000069765.

Structurei

3D structure databases

ProteinModelPortaliP60322.
SMRiP60322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 88C2HC 1Add BLAST28
Motifi96 – 112C2HC 2Add BLAST17

Domaini

The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the nanos family.PROSITE-ProRule annotation
Contains 1 nanos-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 114Nanos-typePROSITE-ProRule annotationAdd BLAST55

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4602. Eukaryota.
ENOG41121KZ. LUCA.
GeneTreeiENSGT00640000091383.
HOGENOMiHOG000082528.
HOVERGENiHBG052575.
InParanoidiP60322.
KOiK18760.
OMAiTICNFCK.
OrthoDBiEOG091G0JUB.
TreeFamiTF326882.

Family and domain databases

InterProiIPR008705. Nanos/Xcar2.
IPR024161. Znf_nanos-typ.
[Graphical view]
PANTHERiPTHR12887. PTHR12887. 1 hit.
PfamiPF05741. zf-nanos. 1 hit.
[Graphical view]
PROSITEiPS51522. ZF_NANOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLPPFDMWR DYFNLSQVVM DIIQSRKQRQ EGEVAEEPNS RPQEKSEQDL
60 70 80 90 100
EGYPGCLPTI CNFCKHNGES RHVYTSHQLK TPEGVVVCPI LRHYVCPLCG
110 120 130
ATGDQAHTLK YCPLNSSQQS LYRRSGRNSA GRRVKR
Length:136
Mass (Da):15,597
Last modified:December 14, 2011 - v2
Checksum:i6D58AF6DD7DD63B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46S → T in BAC82557 (PubMed:12947200).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095972 mRNA. Translation: BAC82557.1.
AC170864 Genomic DNA. No translation available.
CCDSiCCDS20883.1.
RefSeqiNP_918953.2. NM_194064.2.
UniGeneiMm.277153.

Genome annotation databases

EnsembliENSMUST00000063563; ENSMUSP00000069765; ENSMUSG00000051965.
GeneIDi378430.
KEGGimmu:378430.
UCSCiuc009fjz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095972 mRNA. Translation: BAC82557.1.
AC170864 Genomic DNA. No translation available.
CCDSiCCDS20883.1.
RefSeqiNP_918953.2. NM_194064.2.
UniGeneiMm.277153.

3D structure databases

ProteinModelPortaliP60322.
SMRiP60322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237543. 1 interactor.
DIPiDIP-59508N.
IntActiP60322. 7 interactors.
STRINGi10090.ENSMUSP00000069765.

PTM databases

SwissPalmiP60322.

Proteomic databases

PaxDbiP60322.
PRIDEiP60322.

Protocols and materials databases

DNASUi378430.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063563; ENSMUSP00000069765; ENSMUSG00000051965.
GeneIDi378430.
KEGGimmu:378430.
UCSCiuc009fjz.1. mouse.

Organism-specific databases

CTDi339345.
MGIiMGI:2676627. Nanos2.

Phylogenomic databases

eggNOGiKOG4602. Eukaryota.
ENOG41121KZ. LUCA.
GeneTreeiENSGT00640000091383.
HOGENOMiHOG000082528.
HOVERGENiHBG052575.
InParanoidiP60322.
KOiK18760.
OMAiTICNFCK.
OrthoDBiEOG091G0JUB.
TreeFamiTF326882.

Miscellaneous databases

PROiP60322.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051965.
CleanExiMM_NANOS2.
MM_NOS2.
ExpressionAtlasiP60322. baseline and differential.

Family and domain databases

InterProiIPR008705. Nanos/Xcar2.
IPR024161. Znf_nanos-typ.
[Graphical view]
PANTHERiPTHR12887. PTHR12887. 1 hit.
PfamiPF05741. zf-nanos. 1 hit.
[Graphical view]
PROSITEiPS51522. ZF_NANOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANO2_MOUSE
AccessioniPrimary (citable) accession number: P60322
Secondary accession number(s): F8VQ09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: December 14, 2011
Last modified: November 2, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.