P60321 (NANO2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nanos homolog 2
Short name=NOS-2

Gene names

Name:	NANOS2
Synonyms:	NOS2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state By similarity.
Subunit structure	Interacts with CNOT1, CNOT3, CNOT6L, CNOT7 and CNOT9 By similarity.
Subcellular location	Cytoplasm. Cytoplasm › P-body By similarity. Cytoplasm › perinuclear region. Note: Localizes at P-bodies during gonocyte development By similarity. More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis. Ref.2
Tissue specificity	Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level). Ref.2 Ref.3
Developmental stage	Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level). Ref.2 Ref.3
Domain	The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein By similarity.
Sequence similarities	Belongs to the nanos family. Contains 1 nanos-type zinc finger.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	germ-line stem cell maintenance Inferred from sequence or structural similarity. Source: UniProtKB mRNA catabolic process Inferred from sequence or structural similarity. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of meiosis Inferred from sequence or structural similarity. Source: UniProtKB regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasmic mRNA processing body Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay Ref.2. Source: UniProtKB
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 138	138	Nanos homolog 2		PRO_0000207687
Regions
	Zinc finger	62 – 116	55	Nanos-type
	Motif	63 – 90	28	C2HC 1
	Motif	98 – 114	17	C2HC 2
Natural variations
	Natural variant	68	1	H → Q. Ref.2		VAR_065246

Sequences

	Sequence		Length	Mass (Da)
	P60321 [UniParc]. Last modified February 2, 2004. Version 1. Checksum: 98469E0F1AA6BC11	FASTA	138	15,132
	10 20 30 40 50 60 MQLPPFDMWK DYFNLSQVVW ALIASRGQRL ETQEIEEPSP GPPLGQDQGL GAPGANGGLG 70 80 90 100 110 120 TLCNFCKHNG ESRHVYSSHQ LKTPDGVVVC PILRHYVCPV CGATGDQAHT LKYCPLNGGQ 130 QSLYRRSGRN SAGRRVKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction." Kusz K.M., Tomczyk L., Sajek M., Spik A., Latos-Bielenska A., Jedrzejczak P., Pawelczyk L., Jaruzelska J. Mol. Hum. Reprod. 15:165-171(2009) [PubMed: 19168545] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, VARIANT GLN-68.
[3]	"NANOS3 function in human germ cell development." Julaton V.T., Reijo Pera R.A. Hum. Mol. Genet. 20:2238-2250(2011) [PubMed: 21421998] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC042883 mRNA. Translation: AAH42883.1. BC117484 mRNA. Translation: AAI17485.1. BC117486 mRNA. Translation: AAI17487.1.
IPI	IPI00397723.
RefSeq	NP_001025032.1. NM_001029861.2.
UniGene	Hs.434218.
3D structure databases
ProteinModelPortal	P60321.
SMR	P60321. Positions 62-115.
ModBase	Search...
Protein-protein interaction databases
STRING	P60321.
Polymorphism databases
DMDM	41688561.
Proteomic databases
PRIDE	P60321.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000341294; ENSP00000341021; ENSG00000188425.
GeneID	339345.
KEGG	hsa:339345.
UCSC	uc002pdu.1. human.
Organism-specific databases
CTD	339345.
GeneCards	GC19M046416.
HGNC	HGNC:23292. NANOS2.
MIM	608228. gene.
neXtProt	NX_P60321.
PharmGKB	PA134909776.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20114.
GeneTree	ENSGT00390000005985.
HOGENOM	HBG713043.
HOVERGEN	HBG052575.
InParanoid	P60321.
OMA	HYVCPLC.
OrthoDB	EOG4C87TT.
PhylomeDB	P60321.
Gene expression databases
ArrayExpress	P60321.
Bgee	P60321.
CleanEx	HS_NANOS2. HS_NOS2.
Genevestigator	P60321.
GermOnline	ENSG00000188425. Homo sapiens.
Family and domain databases
InterPro	IPR008705. Nanos/Xcar2. IPR024161. Znf_nanos-typ. [Graphical view]
PANTHER	PTHR12887. Znf_Nanos. 1 hit.
Pfam	PF05741. zf-nanos. 1 hit. [Graphical view]
PROSITE	PS51522. ZF_NANOS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	97363.
SOURCE	Search...

Entry information

Entry name

NANO2_HUMAN

Accession

Primary (citable) accession number: P60321
Secondary accession number(s): Q17R30, Q4G0P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	February 2, 2004
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.