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Protein

Cytotoxin 3

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Basic protein that binds to cell membrane and depolarizes cardiomyocytes. This cytotoxin also possesses lytic activity on many other cells, including red blood cells (PubMed:8182052). Interaction with sulfatides in the cell membrane induces pore formation and cell internalization. Cytotoxicity is due to pore formation, and to another mechanism independent of membrane-damaging activity. When internalized, it targets the mitochondrial membrane and induces mitochondrial swelling and fragmentation. It inhibits protein kinases C. It binds to the integrin alpha-V/beta-3 (ITGAV/ITGB3) with a moderate affinity (PubMed:16407244). It also binds with high affinity to heparin (PubMed:17685633).7 Publications

Miscellaneous

Is classified as a P-type cytotoxin, since a proline residue stands at position 30 (Pro-31 in standard classification).1 Publication

GO - Biological processi

Keywordsi

Molecular functionCardiotoxin, Toxin
Biological processCytolysis, Hemolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Cytotoxin 3
Alternative name(s):
Cardiotoxin 31 Publication
Short name:
CTX-3
Short name:
CTX3
Cardiotoxin A32 Publications
Short name:
CTX A32 Publications
Cardiotoxin III1 Publication
Cardiotoxin analog III
Short name:
CTX III
OrganismiNaja atra (Chinese cobra)
Taxonomic identifieri8656 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Secreted, Target cell membrane, Target membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 213 PublicationsAdd BLAST21
ChainiPRO_000003537022 – 81Cytotoxin 3Add BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 424 Publications
Disulfide bondi35 ↔ 594 Publications
Disulfide bondi63 ↔ 744 Publications
Disulfide bondi75 ↔ 804 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Monomer in solution; Homodimer and oligomer in the presence of negatively charged lipids forming a pore with a size ranging between 20 and 30 Angstroms (PubMed:16263708,PubMed:12660250). Interacts with Kv channel-interacting protein 1 (KCNIP1) in a calcium-independent manner (PubMed:15184042).3 Publications

Structurei

Secondary structure

181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 25Combined sources3
Beta strandi32 – 34Combined sources3
Beta strandi36 – 38Combined sources3
Beta strandi41 – 47Combined sources7
Beta strandi49 – 51Combined sources3
Beta strandi56 – 62Combined sources7
Beta strandi68 – 75Combined sources8
Beta strandi77 – 79Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H0JX-ray1.90A/B/C22-81[»]
1I02NMR-A22-81[»]
1XT3X-ray2.40A/B22-81[»]
2BHIX-ray2.31A/B22-81[»]
2CRSNMR-A22-81[»]
2CRTNMR-A22-81[»]
ProteinModelPortaliP60301.
SMRiP60301.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60301.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006553.

Family and domain databases

CDDicd00206. snake_toxin. 1 hit.
InterProiView protein in InterPro
IPR003572. Cytotoxin.
IPR003571. Snake_3FTx.
IPR018354. Snake_toxin_con_site.
IPR035076. Toxin/TOLIP.
PfamiView protein in Pfam
PF00087. Toxin_TOLIP. 1 hit.
PRINTSiPR00282. CYTOTOXIN.
PROSITEiView protein in PROSITE
PS00272. SNAKE_TOXIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60301-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLLLTLVV VTIVCLDLGY TLKCNKLVPL FYKTCPAGKN LCYKMFMVAT
60 70 80
PKVPVKRGCI DVCPKSSLLV KYVCCNTDRC N
Length:81
Mass (Da):9,038
Last modified:February 2, 2004 - v1
Checksum:i70FE0A82FAC9DC95
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 11VVV → EEE in CAB42055 (PubMed:10708798).Curated3
Sequence conflicti59C → R in CAA90963 (PubMed:8619792).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54227 mRNA. Translation: CAA90963.1.
U58486 mRNA. Translation: AAB18382.1.
U42585 mRNA. Translation: AAB01541.1.
AJ007796 mRNA. Translation: CAA07686.1.
AJ238735 Genomic DNA. Translation: CAB42055.1.
PIRiJC4620. H3NJ3F.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry namei3SA3_NAJAT
AccessioniPrimary (citable) accession number: P60301
Secondary accession number(s): P01444, Q9PS23, Q9W6W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 2, 2004
Last modified: July 5, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families