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Protein

Chromosome partition protein MukF

Gene

mukF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, DNA condensation

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciEcoCyc:EG12165-MONOMER.
ECOL316407:JW0905-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome partition protein MukF
Alternative name(s):
Protein KicB
Gene namesi
Name:mukF
Synonyms:kicB
Ordered Locus Names:b0922, JW0905
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12165. mukF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi233L → P: Abolishes function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002116001 – 440Chromosome partition protein MukFAdd BLAST440

Proteomic databases

PaxDbiP60293.
PRIDEiP60293.

Interactioni

Subunit structurei

Interacts, and probably forms a ternary complex, with MukE and MukB via its C-terminal region. The complex formation is stimulated by calcium or magnesium. It is required for an interaction between MukE and MukB.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mukEP225244EBI-554679,EBI-554672

Protein-protein interaction databases

BioGridi4260007. 248 interactors.
DIPiDIP-39981N.
IntActiP60293. 3 interactors.
MINTiMINT-1300138.
STRINGi511145.b0922.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 18Combined sources9
Beta strandi22 – 24Combined sources3
Helixi26 – 40Combined sources15
Helixi49 – 62Combined sources14
Helixi70 – 83Combined sources14
Beta strandi86 – 89Combined sources4
Beta strandi101 – 103Combined sources3
Helixi105 – 115Combined sources11
Helixi122 – 144Combined sources23
Helixi149 – 155Combined sources7
Helixi157 – 162Combined sources6
Helixi164 – 195Combined sources32
Helixi197 – 241Combined sources45
Beta strandi242 – 245Combined sources4
Helixi247 – 279Combined sources33
Turni288 – 291Combined sources4
Helixi297 – 306Combined sources10
Helixi308 – 310Combined sources3
Beta strandi313 – 318Combined sources6
Beta strandi323 – 325Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T98X-ray2.90A/B1-287[»]
3EUHX-ray2.90A/B1-440[»]
3RPUX-ray3.60A/B/X1-440[»]
ProteinModelPortaliP60293.
SMRiP60293.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60293.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni208 – 236Leucine-zipperAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi325 – 356Asp/Glu-rich (acidic)Add BLAST32

Sequence similaritiesi

Belongs to the MukF family.Curated

Phylogenomic databases

eggNOGiENOG4105GHU. Bacteria.
COG3006. LUCA.
HOGENOMiHOG000278265.
KOiK03633.
OMAiNRVFSQR.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_01803. MukF. 1 hit.
InterProiIPR005582. Chromosome_partition_MukF.
IPR033441. MukF_C.
IPR033440. MukF_M.
IPR033439. MukF_WHTH.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03882. KicB. 1 hit.
PF17193. MukF_C. 1 hit.
PF17192. MukF_M. 1 hit.
[Graphical view]
PIRSFiPIRSF018282. MukF. 1 hit.
SUPFAMiSSF140570. SSF140570. 1 hit.
SSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

P60293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG
60 70 80 90 100
ELVDAFRHVS DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI
110 120 130 140 150
YRLTPLGIGI TDYYIRQREF STLRLSMQLS IVAGELKRAA DAAEEGGDEF
160 170 180 190 200
HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE QQQQVKDDIA QLLNKDWRAA
210 220 230 240 250
ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT MTHDDLHFVD
260 270 280 290 300
RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR
310 320 330 340 350
LRQSVQTYFD EPWALTYANA DRLLDMRDEE MALRDEEVTG ELPEDLEYEE
360 370 380 390 400
FNEIREQLAA IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI
410 420 430 440
VIDQAVRLGV AQADFTGLPA KWQPINDYGA KVQAHVIDKY
Length:440
Mass (Da):50,579
Last modified:January 16, 2004 - v1
Checksum:i55824FD545247EE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26440 Genomic DNA. Translation: BAA05457.1.
U00096 Genomic DNA. Translation: AAC74008.1.
AP009048 Genomic DNA. Translation: BAA35668.1.
PIRiS43911.
RefSeqiNP_415442.1. NC_000913.3.
WP_001288850.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74008; AAC74008; b0922.
BAA35668; BAA35668; BAA35668.
GeneIDi945548.
KEGGiecj:JW0905.
eco:b0922.
PATRICi32117057. VBIEscCol129921_0953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26440 Genomic DNA. Translation: BAA05457.1.
U00096 Genomic DNA. Translation: AAC74008.1.
AP009048 Genomic DNA. Translation: BAA35668.1.
PIRiS43911.
RefSeqiNP_415442.1. NC_000913.3.
WP_001288850.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T98X-ray2.90A/B1-287[»]
3EUHX-ray2.90A/B1-440[»]
3RPUX-ray3.60A/B/X1-440[»]
ProteinModelPortaliP60293.
SMRiP60293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260007. 248 interactors.
DIPiDIP-39981N.
IntActiP60293. 3 interactors.
MINTiMINT-1300138.
STRINGi511145.b0922.

Proteomic databases

PaxDbiP60293.
PRIDEiP60293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74008; AAC74008; b0922.
BAA35668; BAA35668; BAA35668.
GeneIDi945548.
KEGGiecj:JW0905.
eco:b0922.
PATRICi32117057. VBIEscCol129921_0953.

Organism-specific databases

EchoBASEiEB2084.
EcoGeneiEG12165. mukF.

Phylogenomic databases

eggNOGiENOG4105GHU. Bacteria.
COG3006. LUCA.
HOGENOMiHOG000278265.
KOiK03633.
OMAiNRVFSQR.

Enzyme and pathway databases

BioCyciEcoCyc:EG12165-MONOMER.
ECOL316407:JW0905-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP60293.
PROiP60293.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_01803. MukF. 1 hit.
InterProiIPR005582. Chromosome_partition_MukF.
IPR033441. MukF_C.
IPR033440. MukF_M.
IPR033439. MukF_WHTH.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03882. KicB. 1 hit.
PF17193. MukF_C. 1 hit.
PF17192. MukF_M. 1 hit.
[Graphical view]
PIRSFiPIRSF018282. MukF. 1 hit.
SUPFAMiSSF140570. SSF140570. 1 hit.
SSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMUKF_ECOLI
AccessioniPrimary (citable) accession number: P60293
Secondary accession number(s): P36567
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a killing factor. PubMed:8602138 showed that it is not involved in killing system.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.