Chromosome partition protein MukF - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P60293 (MUKF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chromosome partition protein MukF

Alternative name(s):
Protein KicB

Gene names

Name:	mukF
Synonyms:	kicB
Ordered Locus Names:	b0922, JW0905

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity. Ref.6
Subunit structure	Interacts, and probably forms a ternary complex, with MukE and MukB via its C-terminal region. The complex formation is stimulated by calcium or magnesium. It is required for an interaction between MukE and MukB. Ref.5 Ref.7
Subcellular location	Cytoplasm › nucleoid. Note: Restricted to the nucleoid region. HAMAP-Rule MF_01803
Sequence similarities	Belongs to the MukF family.
Caution	Was originally thought to be a killing factor. Ref.6 showed that it is not involved in killing system.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition DNA condensation
Cellular component	Cytoplasm
Ligand	Calcium
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: HAMAP nucleoid Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
mukE	P22524	4	EBI-554679,EBI-554672

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 440

440

Chromosome partition protein MukF HAMAP-Rule MF_01803

PRO_0000211600

Regions

Region

208 – 236

Leucine-zipper HAMAP-Rule MF_01803

Compositional bias

325 – 356

Asp/Glu-rich (acidic) HAMAP-Rule MF_01803

Experimental info

Mutagenesis

233

L → P: Abolishes function. Ref.6

Secondary structure

440

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P60293 [UniParc].

Last modified January 16, 2004. Version 1.
Checksum: 55824FD545247EE8
Show »

FASTA

440

50,579

        10         20         30         40         50         60 
MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG ELVDAFRHVS 

        70         80         90        100        110        120 
DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI YRLTPLGIGI TDYYIRQREF 

       130        140        150        160        170        180 
STLRLSMQLS IVAGELKRAA DAAEEGGDEF HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE 

       190        200        210        220        230        240 
QQQQVKDDIA QLLNKDWRAA ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT 

       250        260        270        280        290        300 
MTHDDLHFVD RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR 

       310        320        330        340        350        360 
LRQSVQTYFD EPWALTYANA DRLLDMRDEE MALRDEEVTG ELPEDLEYEE FNEIREQLAA 

       370        380        390        400        410        420 
IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI VIDQAVRLGV AQADFTGLPA 

       430        440 
KWQPINDYGA KVQAHVIDKY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New killing system controlled by two genes located immediately upstream of the mukB gene in Escherichia coli." Feng J., Yamanaka K., Niki H., Ogura T., Hiraga S. Mol. Gen. Genet. 243:136-147(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction." Li Y., Stewart N.K., Berger A.J., Vos S., Schoeffler A.J., Berger J.M., Chait B.T., Oakley M.G. Proc. Natl. Acad. Sci. U.S.A. 107:18832-18837(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 162-176 AND 263-279, INTERACTION WITH MUKB.
[6]	"Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli." Yamanaka K., Ogura T., Niki H., Hiraga S. Mol. Gen. Genet. 250:241-251(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LEU-233.
[7]	"Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli." Yamazoe M., Onogi T., Sunako Y., Niki H., Yamanaka K., Ichimura T., Hiraga S. EMBO J. 18:5873-5884(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, INTERACTION WITH MUKB AND MUKE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D26440 Genomic DNA. Translation: BAA05457.1.
U00096 Genomic DNA. Translation: AAC74008.1.
AP009048 Genomic DNA. Translation: BAA35668.1.

PIR

S43911.

RefSeq

NP_415442.1. NC_000913.2.
YP_489194.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T98	X-ray	2.90	A/B	1-287	[»]
3EUH	X-ray	2.90	A/B	1-440	[»]
3RPU	X-ray	3.60	A/B/X	1-440	[»]

ProteinModelPortal

P60293.

SMR

P60293. Positions 7-440.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-39981N.

IntAct

P60293. 3 interactions.

MINT

MINT-1300138.

STRING

511145.b0922.

Proteomic databases

PaxDb

P60293.

PRIDE

P60293.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74008; AAC74008; b0922.
BAA35668; BAA35668; BAA35668.

GeneID

12931013.
945548.

KEGG

ecj:Y75_p0894.
eco:b0922.

PATRIC

32117057. VBIEscCol129921_0953.

Organism-specific databases

EchoBASE

EB2084.

EcoGene

EG12165. mukF.

Phylogenomic databases

eggNOG

COG3006.

HOGENOM

HOG000278265.

K03633.

OMA

NRFSSEF.

ProtClustDB

PRK05260.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12165-MONOMER.
ECOL316407:JW0905-MONOMER.

Gene expression databases

Genevestigator

P60293.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

HAMAP

MF_01803. MukF.

InterPro

IPR005582. Chromosome_partition_MukF.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF03882. KicB. 1 hit.
[Graphical view]

PIRSF

PIRSF018282. MukF. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P60293.

Entry information

Entry name

MUKF_ECOLI

Accession

Primary (citable) accession number: P60293
Secondary accession number(s): P36567

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents