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Protein

Chromosome partition protein MukF

Gene

mukF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity.1 Publication

Caution

Was originally thought to be a killing factor. PubMed:8602138 showed that it is not involved in killing system.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, DNA condensation
LigandCalcium

Enzyme and pathway databases

BioCyciEcoCyc:EG12165-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosome partition protein MukF
Alternative name(s):
Protein KicB
Gene namesi
Name:mukF
Synonyms:kicB
Ordered Locus Names:b0922, JW0905
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12165 mukF

Subcellular locationi

  • nucleoid
  • Note: Restricted to the nucleoid region.

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi233L → P: Abolishes function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002116001 – 440Chromosome partition protein MukFAdd BLAST440

Proteomic databases

PaxDbiP60293
PRIDEiP60293

Interactioni

Subunit structurei

Interacts, and probably forms a ternary complex, with MukE and MukB via its C-terminal region. The complex formation is stimulated by calcium or magnesium. It is required for an interaction between MukE and MukB.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mukEP225246EBI-554679,EBI-554672

Protein-protein interaction databases

BioGridi4260007, 248 interactors
DIPiDIP-39981N
IntActiP60293, 3 interactors
MINTiP60293
STRINGi316385.ECDH10B_0992

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 18Combined sources9
Beta strandi22 – 24Combined sources3
Helixi26 – 40Combined sources15
Helixi49 – 62Combined sources14
Helixi70 – 83Combined sources14
Beta strandi86 – 89Combined sources4
Beta strandi101 – 103Combined sources3
Helixi105 – 115Combined sources11
Helixi122 – 144Combined sources23
Helixi149 – 155Combined sources7
Helixi157 – 162Combined sources6
Helixi164 – 195Combined sources32
Helixi197 – 241Combined sources45
Beta strandi242 – 245Combined sources4
Helixi247 – 279Combined sources33
Turni288 – 291Combined sources4
Helixi297 – 306Combined sources10
Helixi308 – 310Combined sources3
Beta strandi313 – 318Combined sources6
Beta strandi323 – 325Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T98X-ray2.90A/B1-287[»]
3EUHX-ray2.90A/B1-440[»]
3RPUX-ray3.60A/B/X1-440[»]
ProteinModelPortaliP60293
SMRiP60293
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60293

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni208 – 236Leucine-zipperAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi325 – 356Asp/Glu-rich (acidic)Add BLAST32

Sequence similaritiesi

Belongs to the MukF family.Curated

Phylogenomic databases

eggNOGiENOG4105GHU Bacteria
COG3006 LUCA
HOGENOMiHOG000278265
KOiK03633
OMAiLLRIQDC

Family and domain databases

CDDicd16337 MukF_C, 1 hit
Gene3Di1.10.10.10, 1 hit
1.10.225.40, 1 hit
1.20.58.590, 1 hit
HAMAPiMF_01803 MukF, 1 hit
InterProiView protein in InterPro
IPR005582 Chromosome_partition_MukF
IPR033441 MukF_C
IPR038198 MukF_C_sf
IPR033440 MukF_M
IPR036141 MukF_M_sp
IPR033439 MukF_WHTH
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF03882 KicB, 1 hit
PF17193 MukF_C, 1 hit
PF17192 MukF_M, 1 hit
PIRSFiPIRSF018282 MukF, 1 hit
SUPFAMiSSF140570 SSF140570, 1 hit
SSF46785 SSF46785, 1 hit

Sequencei

Sequence statusi: Complete.

P60293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG
60 70 80 90 100
ELVDAFRHVS DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI
110 120 130 140 150
YRLTPLGIGI TDYYIRQREF STLRLSMQLS IVAGELKRAA DAAEEGGDEF
160 170 180 190 200
HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE QQQQVKDDIA QLLNKDWRAA
210 220 230 240 250
ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT MTHDDLHFVD
260 270 280 290 300
RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR
310 320 330 340 350
LRQSVQTYFD EPWALTYANA DRLLDMRDEE MALRDEEVTG ELPEDLEYEE
360 370 380 390 400
FNEIREQLAA IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI
410 420 430 440
VIDQAVRLGV AQADFTGLPA KWQPINDYGA KVQAHVIDKY
Length:440
Mass (Da):50,579
Last modified:January 16, 2004 - v1
Checksum:i55824FD545247EE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26440 Genomic DNA Translation: BAA05457.1
U00096 Genomic DNA Translation: AAC74008.1
AP009048 Genomic DNA Translation: BAA35668.1
PIRiS43911
RefSeqiNP_415442.1, NC_000913.3
WP_001288850.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74008; AAC74008; b0922
BAA35668; BAA35668; BAA35668
GeneIDi945548
KEGGiecj:JW0905
eco:b0922
PATRICifig|1411691.4.peg.1354

Similar proteinsi

Entry informationi

Entry nameiMUKF_ECOLI
AccessioniPrimary (citable) accession number: P60293
Secondary accession number(s): P36567
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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