P60291 (RPON_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit N
EC=2.7.7.6

Gene names

Name:	rpoN
Ordered Locus Names:	PYRAB05340
ORF Names:	PAB7131

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	65 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00250
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00250
Sequence similarities	Belongs to the archaeal rpoN/eukaryotic RPB10 RNA polymerase subunit family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 65	65	DNA-directed RNA polymerase subunit N HAMAP MF_00250		PRO_0000121356
Sites
	Metal binding	7	1	Zinc By similarity
	Metal binding	10	1	Zinc By similarity
	Metal binding	44	1	Zinc By similarity
	Metal binding	45	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60291 [UniParc]. Last modified January 16, 2004. Version 1. Checksum: 3369175FAE484D1D	FASTA	65	7,787
	10 20 30 40 50 60 MIVPVRCFTC GKVIGDKYYE FKRRVEAGED PEKVLDDLGL ERYCCRRMLL SHVELIDDIM HYRVY

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248284 Genomic DNA. Translation: CAB49456.1.
PIR	A75172.
RefSeq	NP_126225.1. NC_000868.1.
3D structure databases
ProteinModelPortal	P60291.
SMR	P60291. Positions 1-62.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003392; EBPYRP00000003323; EBPYRG00000003392.
GeneID	1495436.
GenomeReviews	Gene locus PYRAB05340 in contig AL096836_GR.
KEGG	pab:PAB7131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022879.
HOGENOM	HBG391971.
OMA	KVVGDKW.
PhylomeDB	P60291.
ProtClustDB	PRK04016.
Enzyme and pathway databases
BioCyc	PABY272844:PAB7131-MONOMER.
Family and domain databases
HAMAP	MF_00250. RNApol_arch_N. [Tree]
InterPro	IPR012287. Homeodomain-rel. IPR000268. RNA_pol_su_N/RPB10. IPR023580. RNA_pol_su_RPB10. IPR020789. RNA_pol_suN_Zn-BS. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K03058.
PANTHER	PTHR23413:SF2. RNA_pol_N/8_sub. 1 hit.
Pfam	PF01194. RNA_pol_N. 1 hit. [Graphical view]
PIRSF	PIRSF005653. RNA_pol_N/8_sub. 1 hit.
ProDom	PD006539. RNA_pol_N/8_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF46924. RNA_pol_N/8_sub. 1 hit.
PROSITE	PS01112. RNA_POL_N_8KD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPON_PYRAB

Accession

Primary (citable) accession number: P60291
Secondary accession number(s): Q9V194

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program