Reviewed, UniProtKB/Swiss-Prot P60245 (CXP3F_CONPU)

Names and origin

Protein names	Recommended name:     Psi-conotoxin PIIIF
Organism	Conus purpurascens (Purple cone)
Taxonomic identifier	41690 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Mollusca › Gastropoda › Orthogastropoda › Apogastropoda › Caenogastropoda › Sorbeoconcha › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	24 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts on postsynaptic membranes, binds to the nicotinic acetylcholine receptors (nAChR) and thus inhibits them. Is less toxic than Psi-conotoxin PIIIE.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Sequence similarities	Belongs to the conotoxin M-4 superfamily. Psi-type family.
Mass spectrometry	Molecular mass is 2667.68 Da from positions 1 - 24. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Acetylcholine receptor inhibitor Neurotoxin Postsynaptic neurotoxin Toxin
PTM	Amidation Disulfide bond Hydroxylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW synaptic transmission Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetylcholine receptor inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 24

24

Psi-conotoxin PIIIF

PRO_0000044498

Amino acid modifications

Modified residue

2

1

4-hydroxyproline

Modified residue

3

1

4-hydroxyproline

Modified residue

14

1

4-hydroxyproline

Modified residue

24

1

Lysine amide

Disulfide bond

4 ↔ 16

Ref.1

Disulfide bond

5 ↔ 21

Ref.1

Disulfide bond

10 ↔ 22

Ref.1

Secondary structure

1

.

.

.

24

Helix Strand Turn

Details...

Turn

14 – 18

5

Sequences

	Sequence		Length	Mass (Da)
	P60245-1 [UniParc]. Last modified January 16, 2004. Version 1. Checksum: 6DE39416A9308C8A	FASTA	24	2,609
	10 20 GPPCCLYGSC RPFPGCYNAL CCRK

References

[1]

"Characterization and three-dimensional structure determination of psi-conotoxin PIIIF, a novel noncompetitive antagonist of nicotinic acetylcholine receptors." Van Wagoner R.M., Jacobsen R.B., Olivera B.M., Ireland C.M. Biochemistry 42:6353-6362(2003) [PubMed: 12767216] [Abstract] Cited for: PROTEIN SEQUENCE, SYNTHESIS, MASS SPECTROMETRY, STRUCTURE BY NMR, DISULFIDE BONDS. Tissue: Venom.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JLP	NMR	-	A	1-24	[»]

ModBase

Search...

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

CXP3F_CONPU

Accession

Primary (citable) accession number: P60245

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)