P60229 (EIF3E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 3 subunit E
Short name=eIF3e

Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6
MMTV integration site 6
Mammary tumor-associated protein INT-6
Viral integration site protein INT-6
eIF-3 p48

Gene names

Name:	Eif3e
Synonyms:	Eif3s6, Int6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins By similarity. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. HAMAP-Rule MF_03004
Subunit structure	Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 By similarity. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with IFIT1 and IFIT2 By similarity. Ref.5
Subcellular location	Cytoplasm By similarity. Nucleus › PML body By similarity.
Tissue specificity	Ubiquitously expressed.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. HAMAP-Rule MF_03004
Involvement in disease	Int-6 serves as a site for viral integration of mouse mammary tumor virus (MMTV) in mammary tumors.
Sequence similarities	Belongs to the eIF-3 subunit E family. Contains 1 PCI domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm Nucleus
Molecular function	Initiation factor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from sequence or structural similarity. Source: UniProtKB translational initiation Inferred from direct assay Ref.5. Source: UniProtKB
Cellular_component	PML body Inferred from electronic annotation. Source: UniProtKB-SubCell eukaryotic translation initiation factor 3 complex Inferred from direct assay Ref.5. Source: UniProtKB
Molecular_function	translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 445	444	Eukaryotic translation initiation factor 3 subunit E HAMAP-Rule MF_03004		PRO_0000123516
Regions
	Domain	290 – 395	106	PCI
	Region	4 – 128	125	Sufficient for interaction with EPAS1 By similarity
	Region	9 – 195	187	Sufficient for interaction with TRIM27 By similarity
	Region	351 – 445	95	Sufficient for interaction with MCM7 By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	275	1	N6-acetyllysine By similarity
	Modified residue	399	1	Phosphoserine By similarity
	Modified residue	439	1	Phosphothreonine Ref.6
	Modified residue	445	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	158 – 180	23	VPATD…EILMQ → LPQSADLRATGVCGGTLWKV MDK in MMTV-induced tumor 1139-2.
	Natural variant	158	1	V → N in MMTV-induced tumor 1139-1.
	Natural variant	159 – 445	287	Missing in MMTV-induced tumor 1139-1.
	Natural variant	318 – 365	48	VLVND…NMTPE → LKIKFFQSKSGIRYVNQAVS GFMEIHRAAVGRPEGHRGLR GDPLEGNG in MMTV-induced tumor 22.
	Natural variant	366 – 445	80	Missing in MMTV-induced tumor 22.

Sequences

	Sequence		Length	Mass (Da)
	P60229 [UniParc]. Last modified January 16, 2004. Version 1. Checksum: A5368651DD0DDD0C	FASTA	445	52,221
	10 20 30 40 50 60 MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN 70 80 90 100 110 120 LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET TRQMQSTRDG RMLFDYLADK 130 140 150 160 170 180 HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY LYFFRVLVPA TDRNALSSLW GKLASEILMQ 190 200 210 220 230 240 NWDAAMEDLT RLKETIDNNS VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP 250 260 270 280 290 300 QYLNAIQTMC PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY 310 320 330 340 350 360 VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC ISINMLADKL 370 380 390 400 410 420 NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP YQQVIEKTKS LSFRSQMLAM 430 440 NIEKKLNQNS RSEAPNWATQ DSGFY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Int-6, a highly conserved, widely expressed gene, is mutated by mouse mammary tumor virus in mammary preneoplasia." Marchetti A., Buttitta F., Miyazaki S., Gallahan D., Smith G.H., Callahan R. J. Virol. 69:1932-1938(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of the INT6 mammary tumor gene product." Diella F., Levi G., Callahan R. DNA Cell Biol. 16:839-847(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	Callahan R. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[5]	"Reconstitution reveals the functional core of mammalian eIF3." Masutani M., Sonenberg N., Yokoyama S., Imataka H. EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX, IDENTIFICATION IN THE EIF-3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-439, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S75221 Genomic DNA. Translation: AAC00046.1. S75223 Genomic DNA. Translation: AAC00047.1. BC029177 mRNA. Translation: AAH29177.1.
IPI	IPI00132250.
RefSeq	NP_032414.1. NM_008388.2.
UniGene	Mm.289992.
3D structure databases
ProteinModelPortal	P60229.
SMR	P60229. Positions 326-399.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-44570N.
IntAct	P60229. 1 interaction.
MINT	MINT-1869759.
PTM databases
PhosphoSite	P60229.
Proteomic databases
PaxDb	P60229.
PRIDE	P60229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336.
GeneID	16341.
KEGG	mmu:16341.
Organism-specific databases
CTD	3646.
MGI	MGI:99257. Eif3e.
Phylogenomic databases
eggNOG	NOG324642.
HOGENOM	HOG000036697.
HOVERGEN	HBG001097.
InParanoid	P60229.
KO	K03250.
OMA	FLEHLYV.
OrthoDB	EOG4CG083.
Gene expression databases
ArrayExpress	P60229.
Bgee	P60229.
Genevestigator	P60229.
GermOnline	ENSMUSG00000022336. Mus musculus.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_03004. eIF3e.
InterPro	IPR019010. eIF3_su6_N. IPR000717. PCI_dom. IPR016650. Transl_init_fac_3_su6_euk. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF09440. eIF3_N. 1 hit. PF01399. PCI. 1 hit. [Graphical view]
PIRSF	PIRSF016255. eIF3e_su6. 1 hit.
SMART	SM00088. PINT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	EIF3E. mouse.
NextBio	289442.
SOURCE	Search...

Entry information

Entry name

EIF3E_MOUSE

Accession

Primary (citable) accession number: P60229
Secondary accession number(s): O43902 , Q64058, Q64059, Q64252

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program