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P60229

- EIF3E_MOUSE

UniProt

P60229 - EIF3E_MOUSE

Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

Eif3e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (16 Jan 2004)
      Previous versions | rss
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    Functioni

    Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins By similarity. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.By similarity

    GO - Molecular functioni

    1. translation initiation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
    2. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
    3. regulation of translational initiation Source: UniProtKB-HAMAP
    4. translational initiation Source: UniProtKB

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 3 subunit EUniRule annotation
    Short name:
    eIF3eUniRule annotation
    Alternative name(s):
    Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
    MMTV integration site 6
    Mammary tumor-associated protein INT-6
    Viral integration site protein INT-6
    eIF-3 p48UniRule annotation
    Gene namesi
    Name:Eif3e
    Synonyms:Eif3s6, Int6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:99257. Eif3e.

    Subcellular locationi

    Cytoplasm UniRule annotation. NucleusPML body UniRule annotation

    GO - Cellular componenti

    1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
    2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
    3. eukaryotic translation initiation factor 3 complex Source: UniProtKB
    4. PML body Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Int-6 serves as a site for viral integration of mouse mammary tumor virus (MMTV) in mammary tumors.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 445444Eukaryotic translation initiation factor 3 subunit EPRO_0000123516Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineUniRule annotation
    Modified residuei399 – 3991PhosphoserineBy similarity
    Modified residuei439 – 4391Phosphothreonine1 Publication
    Modified residuei445 – 4451Phosphotyrosine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP60229.
    PaxDbiP60229.
    PRIDEiP60229.

    PTM databases

    PhosphoSiteiP60229.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Gene expression databases

    ArrayExpressiP60229.
    BgeeiP60229.
    GenevestigatoriP60229.

    Interactioni

    Subunit structurei

    Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 By similarity. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with IFIT1 and IFIT2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200775. 3 interactions.
    DIPiDIP-44570N.
    IntActiP60229. 5 interactions.
    MINTiMINT-1869759.

    Structurei

    3D structure databases

    ProteinModelPortaliP60229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini290 – 395106PCIUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 128125Sufficient for interaction with EPAS1UniRule annotationAdd
    BLAST
    Regioni9 – 195187Sufficient for interaction with TRIM27UniRule annotationAdd
    BLAST
    Regioni351 – 44595Sufficient for interaction with MCM7UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the eIF-3 subunit E family.UniRule annotation
    Contains 1 PCI domain.UniRule annotation

    Phylogenomic databases

    eggNOGiNOG324642.
    HOGENOMiHOG000036697.
    HOVERGENiHBG001097.
    InParanoidiP60229.
    KOiK03250.
    OMAiDILTGKW.
    OrthoDBiEOG7QNVKP.
    PhylomeDBiP60229.
    TreeFamiTF101518.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    HAMAPiMF_03004. eIF3e.
    InterProiIPR016650. eIF3e.
    IPR019010. eIF3e_N.
    IPR000717. PCI_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10317. PTHR10317. 1 hit.
    PfamiPF09440. eIF3_N. 1 hit.
    PF01399. PCI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016255. eIF3e_su6. 1 hit.
    SMARTiSM00088. PINT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P60229-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM    50
    VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET 100
    TRQMQSTRDG RMLFDYLADK HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY 150
    LYFFRVLVPA TDRNALSSLW GKLASEILMQ NWDAAMEDLT RLKETIDNNS 200
    VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP QYLNAIQTMC 250
    PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY 300
    VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC 350
    ISINMLADKL NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP 400
    YQQVIEKTKS LSFRSQMLAM NIEKKLNQNS RSEAPNWATQ DSGFY 445
    Length:445
    Mass (Da):52,221
    Last modified:January 16, 2004 - v1
    Checksum:iA5368651DD0DDD0C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti158 – 18023VPATD…EILMQ → LPQSADLRATGVCGGTLWKV MDK in MMTV-induced tumor 1139-2.
    Add
    BLAST
    Natural varianti158 – 1581V → N in MMTV-induced tumor 1139-1.
    Natural varianti159 – 445287Missing in MMTV-induced tumor 1139-1.
    Add
    BLAST
    Natural varianti318 – 36548VLVND…NMTPE → LKIKFFQSKSGIRYVNQAVS GFMEIHRAAVGRPEGHRGLR GDPLEGNG in MMTV-induced tumor 22.
    Add
    BLAST
    Natural varianti366 – 44580Missing in MMTV-induced tumor 22.
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S75221 Genomic DNA. Translation: AAC00046.1.
    S75223 Genomic DNA. Translation: AAC00047.1.
    BC029177 mRNA. Translation: AAH29177.1.
    CCDSiCCDS27452.1.
    RefSeqiNP_032414.1. NM_008388.2.
    UniGeneiMm.289992.

    Genome annotation databases

    EnsembliENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336.
    GeneIDi16341.
    KEGGimmu:16341.
    UCSCiuc007vpj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S75221 Genomic DNA. Translation: AAC00046.1 .
    S75223 Genomic DNA. Translation: AAC00047.1 .
    BC029177 mRNA. Translation: AAH29177.1 .
    CCDSi CCDS27452.1.
    RefSeqi NP_032414.1. NM_008388.2.
    UniGenei Mm.289992.

    3D structure databases

    ProteinModelPortali P60229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200775. 3 interactions.
    DIPi DIP-44570N.
    IntActi P60229. 5 interactions.
    MINTi MINT-1869759.

    PTM databases

    PhosphoSitei P60229.

    Proteomic databases

    MaxQBi P60229.
    PaxDbi P60229.
    PRIDEi P60229.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022960 ; ENSMUSP00000022960 ; ENSMUSG00000022336 .
    GeneIDi 16341.
    KEGGi mmu:16341.
    UCSCi uc007vpj.1. mouse.

    Organism-specific databases

    CTDi 3646.
    MGIi MGI:99257. Eif3e.

    Phylogenomic databases

    eggNOGi NOG324642.
    HOGENOMi HOG000036697.
    HOVERGENi HBG001097.
    InParanoidi P60229.
    KOi K03250.
    OMAi DILTGKW.
    OrthoDBi EOG7QNVKP.
    PhylomeDBi P60229.
    TreeFami TF101518.

    Miscellaneous databases

    ChiTaRSi EIF3E. mouse.
    NextBioi 289442.
    PROi P60229.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P60229.
    Bgeei P60229.
    Genevestigatori P60229.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    HAMAPi MF_03004. eIF3e.
    InterProi IPR016650. eIF3e.
    IPR019010. eIF3e_N.
    IPR000717. PCI_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10317. PTHR10317. 1 hit.
    Pfami PF09440. eIF3_N. 1 hit.
    PF01399. PCI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016255. eIF3e_su6. 1 hit.
    SMARTi SM00088. PINT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Int-6, a highly conserved, widely expressed gene, is mutated by mouse mammary tumor virus in mammary preneoplasia."
      Marchetti A., Buttitta F., Miyazaki S., Gallahan D., Smith G.H., Callahan R.
      J. Virol. 69:1932-1938(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of the INT6 mammary tumor gene product."
      Diella F., Levi G., Callahan R.
      DNA Cell Biol. 16:839-847(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. Callahan R.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    5. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-445, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Reconstitution reveals the functional core of mammalian eIF3."
      Masutani M., Sonenberg N., Yokoyama S., Imataka H.
      EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX, IDENTIFICATION IN THE EIF-3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-439, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiEIF3E_MOUSE
    AccessioniPrimary (citable) accession number: P60229
    Secondary accession number(s): O43902
    , Q64058, Q64059, Q64252
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: January 16, 2004
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Translation initiation factors
      List of translation initiation factor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3