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Protein

Eukaryotic translation initiation factor 3 subunit E

Gene

Eif3e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiREACT_283953. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_292264. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_292503. Translation initiation complex formation.
REACT_305839. Ribosomal scanning and start codon recognition.
REACT_308559. Formation of a pool of free 40S subunits.
REACT_340387. L13a-mediated translational silencing of Ceruloplasmin expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit EUniRule annotation
Short name:
eIF3eUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6UniRule annotation
MMTV integration site 6
Mammary tumor-associated protein INT-6
Viral integration site protein INT-6
eIF-3 p48UniRule annotation
Gene namesi
Name:Eif3e
Synonyms:Eif3s6, Int6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:99257. Eif3e.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • NucleusPML body UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Int-6 serves as a site for viral integration of mouse mammary tumor virus (MMTV) in mammary tumors.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 445444Eukaryotic translation initiation factor 3 subunit EPRO_0000123516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineUniRule annotation
Modified residuei399 – 3991PhosphoserineBy similarity
Modified residuei439 – 4391Phosphothreonine1 Publication
Modified residuei442 – 4421PhosphoserineBy similarity
Modified residuei445 – 4451Phosphotyrosine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP60229.
PaxDbiP60229.
PRIDEiP60229.

PTM databases

PhosphoSiteiP60229.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiP60229.
ExpressionAtlasiP60229. baseline and differential.
GenevisibleiP60229. MM.

Interactioni

Subunit structurei

Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with IFIT1 and IFIT2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi200775. 3 interactions.
DIPiDIP-44570N.
IntActiP60229. 5 interactions.
MINTiMINT-1869759.
STRINGi10090.ENSMUSP00000022960.

Structurei

3D structure databases

ProteinModelPortaliP60229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini290 – 395106PCIUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 128125Sufficient for interaction with EPAS1UniRule annotationAdd
BLAST
Regioni9 – 195187Sufficient for interaction with TRIM27UniRule annotationAdd
BLAST
Regioni351 – 44595Sufficient for interaction with MCM7UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit E family.UniRule annotation
Contains 1 PCI domain.UniRule annotation

Phylogenomic databases

eggNOGiNOG324642.
GeneTreeiENSGT00390000002661.
HOGENOMiHOG000036697.
HOVERGENiHBG001097.
InParanoidiP60229.
KOiK03250.
OMAiKGTNMVD.
OrthoDBiEOG7QNVKP.
PhylomeDBiP60229.
TreeFamiTF101518.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_03004. eIF3e.
InterProiIPR016650. eIF3e.
IPR019010. eIF3e_N.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10317. PTHR10317. 1 hit.
PfamiPF09440. eIF3_N. 1 hit.
PF01399. PCI. 1 hit.
[Graphical view]
PIRSFiPIRSF016255. eIF3e_su6. 1 hit.
SMARTiSM00088. PINT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM
60 70 80 90 100
VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET
110 120 130 140 150
TRQMQSTRDG RMLFDYLADK HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY
160 170 180 190 200
LYFFRVLVPA TDRNALSSLW GKLASEILMQ NWDAAMEDLT RLKETIDNNS
210 220 230 240 250
VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP QYLNAIQTMC
260 270 280 290 300
PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY
310 320 330 340 350
VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC
360 370 380 390 400
ISINMLADKL NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP
410 420 430 440
YQQVIEKTKS LSFRSQMLAM NIEKKLNQNS RSEAPNWATQ DSGFY
Length:445
Mass (Da):52,221
Last modified:January 16, 2004 - v1
Checksum:iA5368651DD0DDD0C
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti158 – 18023VPATD…EILMQ → LPQSADLRATGVCGGTLWKV MDK in MMTV-induced tumor 1139-2.
Add
BLAST
Natural varianti158 – 1581V → N in MMTV-induced tumor 1139-1.
Natural varianti159 – 445287Missing in MMTV-induced tumor 1139-1.
Add
BLAST
Natural varianti318 – 36548VLVND…NMTPE → LKIKFFQSKSGIRYVNQAVS GFMEIHRAAVGRPEGHRGLR GDPLEGNG in MMTV-induced tumor 22.
Add
BLAST
Natural varianti366 – 44580Missing in MMTV-induced tumor 22.
Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75221 Genomic DNA. Translation: AAC00046.1.
S75223 Genomic DNA. Translation: AAC00047.1.
BC029177 mRNA. Translation: AAH29177.1.
CCDSiCCDS27452.1.
RefSeqiNP_032414.1. NM_008388.2.
UniGeneiMm.289992.

Genome annotation databases

EnsembliENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336.
GeneIDi16341.
KEGGimmu:16341.
UCSCiuc007vpj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S75221 Genomic DNA. Translation: AAC00046.1.
S75223 Genomic DNA. Translation: AAC00047.1.
BC029177 mRNA. Translation: AAH29177.1.
CCDSiCCDS27452.1.
RefSeqiNP_032414.1. NM_008388.2.
UniGeneiMm.289992.

3D structure databases

ProteinModelPortaliP60229.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200775. 3 interactions.
DIPiDIP-44570N.
IntActiP60229. 5 interactions.
MINTiMINT-1869759.
STRINGi10090.ENSMUSP00000022960.

PTM databases

PhosphoSiteiP60229.

Proteomic databases

MaxQBiP60229.
PaxDbiP60229.
PRIDEiP60229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336.
GeneIDi16341.
KEGGimmu:16341.
UCSCiuc007vpj.1. mouse.

Organism-specific databases

CTDi3646.
MGIiMGI:99257. Eif3e.

Phylogenomic databases

eggNOGiNOG324642.
GeneTreeiENSGT00390000002661.
HOGENOMiHOG000036697.
HOVERGENiHBG001097.
InParanoidiP60229.
KOiK03250.
OMAiKGTNMVD.
OrthoDBiEOG7QNVKP.
PhylomeDBiP60229.
TreeFamiTF101518.

Enzyme and pathway databases

ReactomeiREACT_283953. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_292264. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_292503. Translation initiation complex formation.
REACT_305839. Ribosomal scanning and start codon recognition.
REACT_308559. Formation of a pool of free 40S subunits.
REACT_340387. L13a-mediated translational silencing of Ceruloplasmin expression.

Miscellaneous databases

ChiTaRSiEif3e. mouse.
NextBioi289442.
PROiP60229.
SOURCEiSearch...

Gene expression databases

BgeeiP60229.
ExpressionAtlasiP60229. baseline and differential.
GenevisibleiP60229. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_03004. eIF3e.
InterProiIPR016650. eIF3e.
IPR019010. eIF3e_N.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10317. PTHR10317. 1 hit.
PfamiPF09440. eIF3_N. 1 hit.
PF01399. PCI. 1 hit.
[Graphical view]
PIRSFiPIRSF016255. eIF3e_su6. 1 hit.
SMARTiSM00088. PINT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Int-6, a highly conserved, widely expressed gene, is mutated by mouse mammary tumor virus in mammary preneoplasia."
    Marchetti A., Buttitta F., Miyazaki S., Gallahan D., Smith G.H., Callahan R.
    J. Virol. 69:1932-1938(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of the INT6 mammary tumor gene product."
    Diella F., Levi G., Callahan R.
    DNA Cell Biol. 16:839-847(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. Callahan R.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  5. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-445, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Reconstitution reveals the functional core of mammalian eIF3."
    Masutani M., Sonenberg N., Yokoyama S., Imataka H.
    EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX, IDENTIFICATION IN THE EIF-3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-439, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiEIF3E_MOUSE
AccessioniPrimary (citable) accession number: P60229
Secondary accession number(s): O43902
, Q64058, Q64059, Q64252
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: July 22, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.