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P60229 (EIF3E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 3 subunit E

Short name=eIF3e
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 6
MMTV integration site 6
Mammary tumor-associated protein INT-6
Viral integration site protein INT-6
eIF-3 p48
Gene names
Name:Eif3e
Synonyms:Eif3s6, Int6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins By similarity. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. HAMAP-Rule MF_03004

Subunit structure

Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2 By similarity. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with IFIT1 and IFIT2 By similarity. Ref.6

Subcellular location

Cytoplasm By similarity. NucleusPML body By similarity HAMAP-Rule MF_03004.

Tissue specificity

Ubiquitously expressed.

Involvement in disease

Int-6 serves as a site for viral integration of mouse mammary tumor virus (MMTV) in mammary tumors.

Sequence similarities

Belongs to the eIF-3 subunit E family.

Contains 1 PCI domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 445444Eukaryotic translation initiation factor 3 subunit E HAMAP-Rule MF_03004
PRO_0000123516

Regions

Domain290 – 395106PCI
Region4 – 128125Sufficient for interaction with EPAS1 By similarity
Region9 – 195187Sufficient for interaction with TRIM27 By similarity
Region351 – 44595Sufficient for interaction with MCM7 By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3991Phosphoserine By similarity
Modified residue4391Phosphothreonine Ref.7
Modified residue4451Phosphotyrosine Ref.5

Natural variations

Natural variant158 – 18023VPATD…EILMQ → LPQSADLRATGVCGGTLWKV MDK in MMTV-induced tumor 1139-2.
Natural variant1581V → N in MMTV-induced tumor 1139-1.
Natural variant159 – 445287Missing in MMTV-induced tumor 1139-1.
Natural variant318 – 36548VLVND…NMTPE → LKIKFFQSKSGIRYVNQAVS GFMEIHRAAVGRPEGHRGLR GDPLEGNG in MMTV-induced tumor 22.
Natural variant366 – 44580Missing in MMTV-induced tumor 22.

Sequences

Sequence LengthMass (Da)Tools
P60229 [UniParc].

Last modified January 16, 2004. Version 1.
Checksum: A5368651DD0DDD0C

FASTA44552,221
        10         20         30         40         50         60 
MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN 

        70         80         90        100        110        120 
LYSDDIPHAL REKRTTVVAQ LKQLQAETEP IVKMFEDPET TRQMQSTRDG RMLFDYLADK 

       130        140        150        160        170        180 
HGFRQEYLDT LYRYAKFQYE CGNYSGAAEY LYFFRVLVPA TDRNALSSLW GKLASEILMQ 

       190        200        210        220        230        240 
NWDAAMEDLT RLKETIDNNS VSSPLQSLQQ RTWLIHWSLF VFFNHPKGRD NIIDLFLYQP 

       250        260        270        280        290        300 
QYLNAIQTMC PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY 

       310        320        330        340        350        360 
VNFDFDGAQK KLRECESVLV NDFFLVACLE DFIENARLFI FETFCRIHQC ISINMLADKL 

       370        380        390        400        410        420 
NMTPEEAERW IVNLIRNARL DAKIDSKLGH VVMGNNAVSP YQQVIEKTKS LSFRSQMLAM 

       430        440 
NIEKKLNQNS RSEAPNWATQ DSGFY 

« Hide

References

« Hide 'large scale' references
[1]"Int-6, a highly conserved, widely expressed gene, is mutated by mouse mammary tumor virus in mammary preneoplasia."
Marchetti A., Buttitta F., Miyazaki S., Gallahan D., Smith G.H., Callahan R.
J. Virol. 69:1932-1938(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Characterization of the INT6 mammary tumor gene product."
Diella F., Levi G., Callahan R.
DNA Cell Biol. 16:839-847(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]Callahan R.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO N-TERMINUS.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland.
[5]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-445, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Reconstitution reveals the functional core of mammalian eIF3."
Masutani M., Sonenberg N., Yokoyama S., Imataka H.
EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF THE EIF-3 COMPLEX, IDENTIFICATION IN THE EIF-3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-439, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S75221 Genomic DNA. Translation: AAC00046.1.
S75223 Genomic DNA. Translation: AAC00047.1.
BC029177 mRNA. Translation: AAH29177.1.
RefSeqNP_032414.1. NM_008388.2.
UniGeneMm.289992.

3D structure databases

ProteinModelPortalP60229.
SMRP60229. Positions 343-399.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200775. 2 interactions.
DIPDIP-44570N.
IntActP60229. 5 interactions.
MINTMINT-1869759.

PTM databases

PhosphoSiteP60229.

Proteomic databases

PaxDbP60229.
PRIDEP60229.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022960; ENSMUSP00000022960; ENSMUSG00000022336.
GeneID16341.
KEGGmmu:16341.
UCSCuc007vpj.1. mouse.

Organism-specific databases

CTD3646.
MGIMGI:99257. Eif3e.

Phylogenomic databases

eggNOGNOG324642.
HOGENOMHOG000036697.
HOVERGENHBG001097.
InParanoidP60229.
KOK03250.
OMADILTGKW.
OrthoDBEOG7QNVKP.
PhylomeDBP60229.
TreeFamTF101518.

Gene expression databases

ArrayExpressP60229.
BgeeP60229.
GenevestigatorP60229.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
HAMAPMF_03004. eIF3e.
InterProIPR016650. eIF3e.
IPR019010. eIF3e_N.
IPR000717. PCI_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR10317. PTHR10317. 1 hit.
PfamPF09440. eIF3_N. 1 hit.
PF01399. PCI. 1 hit.
[Graphical view]
PIRSFPIRSF016255. eIF3e_su6. 1 hit.
SMARTSM00088. PINT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEIF3E. mouse.
NextBio289442.
PROP60229.
SOURCESearch...

Entry information

Entry nameEIF3E_MOUSE
AccessionPrimary (citable) accession number: P60229
Secondary accession number(s): O43902 expand/collapse secondary AC list , Q64058, Q64059, Q64252
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Translation initiation factors

List of translation initiation factor entries