P60227 (MVHA_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F420-non-reducing hydrogenase subunit A
EC=1.12.99.-

Alternative name(s):
Methyl viologen-reducing hydrogenase subunit alpha
Short name=MVH subunit A

Gene names

Name:	mvhA
Ordered Locus Names:	MTBMA_c15170

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of a complex that provides reducing equivalents for heterodisulfide reductase. Ref.3
Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase is composed of three subunits; mvhA, mvhD and mvhG. It forms a complex with the heterodisulfide reductase (hdr). Ref.2
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 472	471	F420-non-reducing hydrogenase subunit A		PRO_0000199727
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential
	Metal binding	442	1	Nickel Potential
	Metal binding	445	1	Nickel Potential
Experimental info
	Sequence conflict	11	1	R → L AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P60227 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 5993E7AC4BC0579F	FASTA	472	52,839
	10 20 30 40 50 60 MVKLTMEPVT RIEGHAKITV HLDDAGNVED TRLHVMEFRG FEKFLQGRPI EEAPRIVPRI 70 80 90 100 110 120 CGICDVQHHL AAAKAVDACF GFEPDDVLPA AYKMREIMNW GSYMHSHGLH FYFLAAPDFI 130 140 150 160 170 180 AGKDRKTRNV FQIIKDAPDV ALQAIELRKN ALEIVRATGG RPIHPTSSTP GGISTELDDE 190 200 210 220 230 240 TQKDLLQKAQ RNVELAEATL ELAVPIFEEN IDLVNSLGNI ETYHTGLVKN GVWDVYDGIV 250 260 270 280 290 300 RIKDKEGNLF REFKPADYAD TIAEHVKPYS WLKFPYIKDL GYPDGVYRVS PLSRLNVADK 310 320 330 340 350 360 MPDAAPKAQD YFKEFQDKFG YAQQTLLYHW ARLIEVLACA ECAADALEGD LSGEKFPDSL 370 380 390 400 410 420 ERQAGDGVGI VEAPRGTLTH HYTCDENGLI TKANIVVATI QNNPAMEMGI QKVAQDYIKP 430 440 450 460 470 GVEVDDKIFN LMEMVIRAYD PCLSCATHTI DSQMRLATLE VYDSEGDLVK RI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed: 20802048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"H2: heterodisulfide oxidoreductase complex from Methanobacterium thermoautotrophicum. Composition and properties." Setzke E., Hedderich R., Heiden S., Thauer R.K. Eur. J. Biochem. 220:139-148(1994) [PubMed: 8119281] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-18, SUBUNIT, ASSOCIATION WITH HETERODISULFIDE REDUCTASE. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[3]	"Physiological role of the F420-non-reducing hydrogenase (Mvh) from Methanothermobacter marburgensis." Stojanowic A., Mander G.J., Duin E.C., Hedderich R. Arch. Microbiol. 180:194-203(2003) [PubMed: 12856108] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH HETERODISULFIDE REDUCTASE. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001710 Genomic DNA. Translation: ADL59096.1.
RefSeq	YP_003850409.1. NC_014408.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9705226.
GenomeReviews	Gene locus MTBMA_c15170 in contig CP001710_GR.
KEGG	mmg:MTBMA_c15170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EVRGFEK.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
KO	K14126.
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MVHA_METTM

Accession

Primary (citable) accession number: P60227
Secondary accession number(s): D9PXZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 30, 2010
Last modified:	November 16, 2011
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program