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P60211 (KA181_TITOB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin alpha-KTx 18.1
Alternative name(s):
Toxin Tc32
Toxin To32
OrganismTityus obscurus (Amazonian scorpion) (Tityus cambridgei)
Taxonomic identifier184226 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Protein attributes

Sequence length35 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversible blocker of both Kv1.3/KCNA3 potassium channels of human T-lymphocytes (high affinity) and Shaker B potassium channels (very low affinity). Ref.1

Subcellular location

Secreted Ref.2.

Tissue specificity

Expressed by the venom gland. Ref.2

Domain

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similarities

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 18 subfamily.

Mass spectrometry

Molecular mass is 3521.5 Da from positions 1 - 35. Determined by ESI. Ref.1 Ref.2

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3535Potassium channel toxin alpha-KTx 18.1
PRO_0000044921

Amino acid modifications

Disulfide bond7 ↔ 26
Disulfide bond12 ↔ 31
Disulfide bond16 ↔ 33

Secondary structure

.......... 35
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60211 [UniParc].

Last modified January 16, 2004. Version 1.
Checksum: B8BB8B54DC016D33

FASTA353,528
        10         20         30 
TGPQTTCQAA MCEAGCKGLG KSMESCQGDT CKCKA 

« Hide

References

[1]"Two novel toxins from the Amazonian scorpion Tityus cambridgei that block Kv1.3 and Shaker B K(+)-channels with distinctly different affinities."
Batista C.V.F., Gomez-Lagunas F., Rodriguez de la Vega R.C., Hajdu P., Panyi G., Gaspar R., Possani L.D.
Biochim. Biophys. Acta 1601:123-131(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, 3D-STRUCTURE MODELING.
Tissue: Venom.
[2]"Proteomics of the venom from the Amazonian scorpion Tityus cambridgei and the role of prolines on mass spectrometry analysis of toxins."
Batista C.V.F., del Pozo L., Zamudio F.Z., Contreras S., Becerril B., Wanke E., Possani L.D.
J. Chromatogr. B 803:55-66(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
Tissue: Venom.
[3]"Structural and functional characterization of the recombinant form of the kv1.3 channel blocker tc32."
Stehling E.G., Sforca M.L., Zanchin N.I., Pignatelli A., Belluzzi O., Spisni A., Pertinhez T.A.
Submitted (APR-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JP6NMR-A1-35[»]
ProteinModelPortalP60211.
SMRP60211. Positions 1-35.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Other

EvolutionaryTraceP60211.

Entry information

Entry nameKA181_TITOB
AccessionPrimary (citable) accession number: P60211
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references