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Protein

Potassium channel toxin alpha-KTx 18.1

Gene
N/A
Organism
Tityus obscurus (Amazonian scorpion) (Tityus cambridgei)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversible blocker of both Kv1.3/KCNA3 potassium channels (high affinity) and Shaker B (mammalian Kv1.1 analog) potassium channels (very low affinity).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei34 – 341Penetrates into Kv pores (more deeply into Kv1.3 than into Kv1.1)1 Publication

GO - Molecular functioni

  • potassium channel inhibitor activity Source: UniProtKB

GO - Biological processi

  • pathogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 18.1
Alternative name(s):
Toxin Tc321 Publication
Toxin To32Curated
OrganismiTityus obscurus (Amazonian scorpion) (Tityus cambridgei)
Taxonomic identifieri184226 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3535Potassium channel toxin alpha-KTx 18.11 PublicationPRO_0000044921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 261 Publication
Disulfide bondi12 ↔ 311 Publication
Disulfide bondi16 ↔ 331 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

35
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 33Combined sources
Helixi10 – 1910Combined sources
Beta strandi21 – 244Combined sources
Beta strandi26 – 294Combined sources
Beta strandi32 – 343Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JP6NMR-A1-35[»]
ProteinModelPortaliP60211.
SMRiP60211. Positions 1-35.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60211.

Family & Domainsi

Domaini

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).1 Publication

Sequence similaritiesi

Sequencei

Sequence statusi: Complete.

P60211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
TGPQTTCQAA MCEAGCKGLG KSMESCQGDT CKCKA
Length:35
Mass (Da):3,528
Last modified:January 16, 2004 - v1
Checksum:iB8BB8B54DC016D33
GO

Mass spectrometryi

Molecular mass is 3521.5 Da from positions 1 - 35. Determined by ESI. 2 Publications

Cross-referencesi

Web resourcesi

Biological Magnetic Resonance Data Bank

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JP6NMR-A1-35[»]
ProteinModelPortaliP60211.
SMRiP60211. Positions 1-35.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP60211.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Two novel toxins from the Amazonian scorpion Tityus cambridgei that block Kv1.3 and Shaker B K(+)-channels with distinctly different affinities."
    Batista C.V.F., Gomez-Lagunas F., Rodriguez de la Vega R.C., Hajdu P., Panyi G., Gaspar R., Possani L.D.
    Biochim. Biophys. Acta 1601:123-131(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, 3D-STRUCTURE MODELING.
    Tissue: Venom.
  2. "Proteomics of the venom from the Amazonian scorpion Tityus cambridgei and the role of prolines on mass spectrometry analysis of toxins."
    Batista C.V.F., del Pozo L., Zamudio F.Z., Contreras S., Becerril B., Wanke E., Possani L.D.
    J. Chromatogr. B 803:55-66(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
    Tissue: Venom.
  3. "Looking over toxin-K(+) channel interactions. Clues from the structural and functional characterization of alpha-KTx toxin Tc32, a Kv1.3 channel blocker."
    Stehling E.G., Sforca M.L., Zanchin N.I., Oyama S. Jr., Pignatelli A., Belluzzi O., Polverini E., Corsini R., Spisni A., Pertinhez T.A.
    Biochemistry 51:1885-1894(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BOND.

Entry informationi

Entry nameiKA181_TITOB
AccessioniPrimary (citable) accession number: P60211
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: June 24, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Caution

Lacks the dyad motif characteristic of alpha-KTx and generally associated with channel blockage.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.