P60209 (KAX94_MESTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 9.4 Alternative name(s): Bt BTK-2 Toxin BTK-2
Organism	Mesobuthus tamulus (Eastern Indian scorpion) (Buthus tamulus)
Taxonomic identifier	34647 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus

Protein attributes

Sequence length	32 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Blocker of human potassium channel Kv1.1/KCNA1.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 9 subfamily.
Mass spectrometry	Molecular mass is 3452 Da from positions 1 - 32. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 32

32

Potassium channel toxin alpha-KTx 9.4

PRO_0000044948

Amino acid modifications

Disulfide bond

3 ↔ 19

By similarity

Disulfide bond

6 ↔ 24

By similarity

Disulfide bond

10 ↔ 26

By similarity

Secondary structure

1

.

.

.

.

.

.

.

32

Helix Strand Turn

Details...

Helix

5 – 8

4

Beta strand

17 – 20

4

Beta strand

23 – 26

4

Sequences

	Sequence		Length	Mass (Da)
	P60209 [UniParc]. Last modified January 16, 2004. Version 1. Checksum: D472FEEB98E7E97F	FASTA	32	3,456
	10 20 30 VGCAECPMHC KGKMAKPTCE NEVCKCNIGK KD

References

[1]	"BTK-2, a new inhibitor of the Kv1.1 potassium channel purified from Indian scorpion Buthus tamulus." Dhawan R., Varshney A., Mathew M.K., Lala A.K. FEBS Lett. 539:7-13(2003) [PubMed: 12650917] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY, 3D-STRUCTURE MODELING. Tissue: Venom.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KTC	NMR	-	A	1-32	[»]

ProteinModelPortal

P60209.

SMR

P60209. Positions 1-28.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008911. Toxin_6.
[Graphical view]

Pfam

PF05453. Toxin_6. 1 hit.
[Graphical view]

PROSITE

PS01138. SCORP_SHORT_TOXIN. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KAX94_MESTA

Accession

Primary (citable) accession number: P60209

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	November 16, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program