P60202 (MYPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myelin proteolipid protein
Short name=PLP

Alternative name(s):
Lipophilin

Gene names

Name:	Plp1
Synonyms:	Plp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
Subcellular location	Membrane; Multi-pass membrane protein.
Involvement in disease	Defects in Plp1 are the cause of the dysmyelinating diseases Jimpy and Rumpshaker (rsh).
Sequence similarities	Belongs to the myelin proteolipid protein family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Disease	Disease mutation
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell maturation Inferred from mutant phenotype PubMed 11746780. Source: MGI central nervous system myelination Inferred from mutant phenotype PubMed 1694232. Source: MGI integrin-mediated signaling pathway Inferred from electronic annotation. Source: Compara long-chain fatty acid biosynthetic process Inferred from mutant phenotype PubMed 1150661. Source: MGI positive regulation of gene expression Inferred from mutant phenotype PubMed 1373175. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW myelin sheath Inferred from direct assay PubMed 1694232PubMed 20578039. Source: MGI
Molecular_function	structural constituent of myelin sheath Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P60202-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform DM-20 (identifier: P60202-2)

The sequence of this isoform differs from the canonical sequence as follows:
     117-151: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 277	276	Myelin proteolipid protein		PRO_0000159007
Regions
	Topological domain	2 – 9	8	Cytoplasmic Probable
	Transmembrane	10 – 36	27	Helical; Name=1; Probable
	Topological domain	37 – 63	27	Extracellular Probable
	Transmembrane	64 – 88	25	Helical; Name=2; Probable
	Topological domain	89 – 151	63	Cytoplasmic Probable
	Transmembrane	152 – 177	26	Helical; Name=3; Probable
	Topological domain	178 – 233	56	Extracellular Probable
	Transmembrane	234 – 260	27	Helical; Name=4; Probable
	Topological domain	261 – 277	17	Cytoplasmic Probable
Amino acid modifications
	Lipidation	6	1	S-palmitoyl cysteine By similarity
	Lipidation	7	1	S-palmitoyl cysteine By similarity
	Lipidation	10	1	S-palmitoyl cysteine By similarity
	Lipidation	109	1	S-palmitoyl cysteine By similarity
	Lipidation	139	1	S-palmitoyl cysteine By similarity
	Lipidation	141	1	S-palmitoyl cysteine By similarity
	Lipidation	199	1	O-palmitoyl serine By similarity
	Disulfide bond	184 ↔ 228		By similarity
	Disulfide bond	201 ↔ 220		By similarity
Natural variations
	Alternative sequence	117 – 151	35	Missing in isoform DM-20.		VSP_009194
	Natural variant	187	1	I → T in Rumpshaker. Ref.12
	Natural variant	243	1	A → V in Jimpy MSD. Ref.11
Experimental info
	Sequence conflict	70	1	Y → C Ref.4
	Sequence conflict	70	1	Y → C Ref.5
	Sequence conflict	126	1	S → Y in AAA39955. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3C2BC973C3061C38	FASTA	277	30,077
	10 20 30 40 50 60 MGLLECCARC LVGAPFASLV ATGLCFFGVA LFCGCGHEAL TGTEKLIETY FSKNYQDYEY 70 80 90 100 110 120 LINVIHAFQY VIYGTASFFF LYGALLLAEG FYTTGAVRQI FGDYKTTICG KGLSATVTGG 130 140 150 160 170 180 QKGRGSRGQH QAHSLERVCH CLGKWLGHPD KFVGITYALT VVWLLVFACS AVPVYIYFNT 190 200 210 220 230 240 WTTCQSIAFP SKTSASIGSL CADARMYGVL PWNAFPGKVC GSNLLSICKT AEFQMTFHLF 250 260 270 IAAFVGAAAT LVSLLTFMIA ATYNFAVLKL MGRGTKF
	Isoform DM-20 [UniParc]. Checksum: 90DEFE60148DC659	FASTA	242	26,274
	10 20 30 40 50 60 MGLLECCARC LVGAPFASLV ATGLCFFGVA LFCGCGHEAL TGTEKLIETY FSKNYQDYEY 70 80 90 100 110 120 LINVIHAFQY VIYGTASFFF LYGALLLAEG FYTTGAVRQI FGDYKTTICG KGLSATFVGI 130 140 150 160 170 180 TYALTVVWLL VFACSAVPVY IYFNTWTTCQ SIAFPSKTSA SIGSLCADAR MYGVLPWNAF 190 200 210 220 230 240 PGKVCGSNLL SICKTAEFQM TFHLFIAAFV GAAATLVSLL TFMIAATYNF AVLKLMGRGT KF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aberrant splicing of proteolipid protein mRNA in the dysmyelinating jimpy mutant mouse." Hudson L.D., Berndt J.A., Puckett C., Kozak C.A., Lazzarini R.A. Proc. Natl. Acad. Sci. U.S.A. 84:1454-1458(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND DM-20).
[2]	"Myelin proteolipid protein gene structure and its regulation of expression in normal and jimpy mutant mice." Ikenaka K., Furuichi T., Iwasaki Y., Moriguchi A., Okano H., Mikoshiba K. J. Mol. Biol. 199:587-596(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]	"Structure and expression of the mouse myelin proteolipid protein gene." Macklin W.B., Campagnoni C.W., Deininger P.L., Gardinier M.V. J. Neurosci. Res. 18:383-394(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1). Strain: BALB/cByJ.
[4]	"Jimpy mutant mouse: a 74-base deletion in the mRNA for myelin proteolipid protein and evidence for a primary defect in RNA splicing." Nave K.-A., Lai C., Bloom F.E., Milner R.J. Proc. Natl. Acad. Sci. U.S.A. 83:9264-9268(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	"Splice site selection in the proteolipid protein (PLP) gene transcript and primary structure of the DM-20 protein of central nervous system myelin." Nave K.-A., Lai C., Bloom F.E., Milner R.J. Proc. Natl. Acad. Sci. U.S.A. 84:5665-5669(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM DM-20).
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Retina.
[7]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-9; 46-53; 99-122; 145-151 AND 193-229, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[8]	"Developmental expression of the myelin proteolipid protein and basic protein mRNAs in normal and dysmyelinating mutant mice." Sorg B.A., Smith M.M., Campagnoni A.T. J. Neurochem. 49:1146-1154(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 50-277.
[9]	"Characterization of myelin proteolipid mRNAs in normal and jimpy mice." Gardinier M.V., Macklin W.B., Diniak A.J., Deininger P.L. Mol. Cell. Biol. 6:3755-3762(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 176-270.
[10]	"An AG to a GG transition at a splice site in the myelin proteolipid protein gene in jimpy mice results in the removal of an exon." Macklin W.B., Gardinier M.V., King K.D., Kampf K. FEBS Lett. 223:417-421(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 209-232, ALTERNATIVE SPLICING.
[11]	"Conservative amino acid substitution in the myelin proteolipid protein of jimpymsd mice." Gencic S., Hudson L.D. J. Neurosci. 10:117-124(1990) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT JIMPY MSD VAL-243.
[12]	"Uncoupling of hypomyelination and glial cell death by a mutation in the proteolipid protein gene." Schneider A., Montague P., Griffiths I., Fanarraga M., Kennedy P., Brophy P., Nave K.-A. Nature 358:758-761(1992) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT RUMPSHAKER THR-187.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M15442 mRNA. Translation: AAA39951.1. M15442 mRNA. Translation: AAA39952.1. Sequence problems. M15442 mRNA. Translation: AAA39953.1. X07215 , X07216, X07217, X07218, X07219, X07220, X07221 Genomic DNA. Translation: CAA30184.1. M37335 , M37329, M37330, M37331, M37332, M37333, M37334 Genomic DNA. Translation: AAA39954.1. M14674 mRNA. Translation: AAA39955.1. M16472 mRNA. Translation: AAA39950.1. BC027010 mRNA. Translation: AAH27010.1. X06375 Genomic DNA. Translation: CAB40821.1.
IPI	IPI00230610. IPI00263013.
PIR	S34792.
RefSeq	NP_035253.1. NM_011123.2.
UniGene	Mm.1268.
3D structure databases
ProteinModelPortal	P60202.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60202. 1 interaction.
MINT	MINT-4102954.
PTM databases
PhosphoSite	P60202.
Proteomic databases
PaxDb	P60202.
PRIDE	P60202.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033800; ENSMUSP00000033800; ENSMUSG00000031425. ENSMUST00000113085; ENSMUSP00000108708; ENSMUSG00000031425.
GeneID	18823.
KEGG	mmu:18823.
UCSC	uc009ujc.1. mouse.
Organism-specific databases
CTD	5354.
MGI	MGI:97623. Plp1.
Phylogenomic databases
eggNOG	NOG311658.
GeneTree	ENSGT00390000006915.
HOGENOM	HOG000231338.
HOVERGEN	HBG000096.
InParanoid	P60202.
OrthoDB	EOG4B8JDT.
Gene expression databases
ArrayExpress	P60202.
Bgee	P60202.
CleanEx	MM_PLP1.
Genevestigator	P60202.
GermOnline	ENSMUSG00000031425. Mus musculus.
Family and domain databases
InterPro	IPR001614. Myelin_PLP. IPR018237. Myelin_PLP_CS. [Graphical view]
PANTHER	PTHR11683. PTHR11683. 1 hit.
Pfam	PF01275. Myelin_PLP. 1 hit. [Graphical view]
PRINTS	PR00214. MYELINPLP.
SMART	SM00002. PLP. 1 hit. [Graphical view]
PROSITE	PS00575. MYELIN_PLP_1. 1 hit. PS01004. MYELIN_PLP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PLP1. mouse.
NextBio	295188.
SOURCE	Search...

Entry information

Entry name

MYPR_MOUSE

Accession

Primary (citable) accession number: P60202
Secondary accession number(s): P04400, P06905, Q9WUS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program