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P60170 (VSGP_EBOZM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pre-small/secreted glycoprotein
Short name=pre-sGP

Cleaved into the following 2 chains:

  1. Small/secreted glycoprotein
    Short name=sGP
  2. Delta-peptide
Gene names
Name:GP
OrganismZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) [Reference proteome]
Taxonomic identifier128952 [NCBI]
Taxonomic lineageViruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Ebolavirus ›
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils. Ref.8 Ref.9 Ref.10 Ref.11

Delta-peptide does not seem to be involved in activation of primary macrophages. Ref.8 Ref.9 Ref.10 Ref.11

Subunit structure

sGP is a homodimer; disulfide-linked. The homodimers are linked by two disulfide bonds in a parallel orientation. Delta-peptide is a monomer. Ref.7

Subcellular location

Small/secreted glycoprotein: Secreted.

Delta-peptide: Secreted.

Post-translational modification

Pre-sGP is N-glycosylated. This precursor is processed into mature sGP and delta-peptide by host furin or furin-like proteases. The cleavage site corresponds to the furin optimal cleavage sequence [KR]-X-[KR]-R. Both cleavage fragments contain sialic acid, but only the delta-peptide is O-glycosylated.

Sequence similarities

Belongs to the filoviruses glycoprotein family.

RNA editing

Edited at position 295.
Partially edited. RNA editing at this position consists of an insertion of one or two adenine nucleotides. The sequence displayed here is the small secreted glycoprotein, derived from the unedited RNA. The sequence derived from the +1A edited gives rise to the full-length transmembrane glycoprotein GP (AC Q05320), the +2A edited RNA gives rise to the super small secreted glycoprotein ssGP (AC Q9YMG2). Ref.1 Ref.2

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityRNA editing
   DomainSignal
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 364332Pre-small/secreted glycoprotein
PRO_0000037512
Chain33 – 324292Small/secreted glycoprotein
PRO_0000037513
Chain325 – 36440Delta-peptide
PRO_0000037514

Sites

Site324 – 3252Cleavage; by host furin

Amino acid modifications

Glycosylation401N-linked (GlcNAc...); by host Potential
Glycosylation2041N-linked (GlcNAc...); by host Potential
Glycosylation2281N-linked (GlcNAc...); by host Potential
Glycosylation2381N-linked (GlcNAc...); by host Potential
Glycosylation2571N-linked (GlcNAc...); by host Potential
Glycosylation2681N-linked (GlcNAc...); by host Potential
Disulfide bond53Interchain Ref.7
Disulfide bond108 ↔ 135 Ref.7
Disulfide bond121 ↔ 147 Ref.7
Disulfide bond306Interchain Ref.7

Natural variations

Natural variant651S → P in strain: Isolate mouse-adapted.
Natural variant2461S → P in strain: Isolate mouse-adapted.

Experimental info

Mutagenesis3241R → S: Loss of cleavage. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P60170 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 67376A454CE5F362

FASTA36441,175
        10         20         30         40         50         60 
MGVTGILQLP RDRFKRTSFF LWVIILFQRT FSIPLGVIHN STLQVSDVDK LVCRDKLSST 

        70         80         90        100        110        120 
NQLRSVGLNL EGNGVATDVP SATKRWGFRS GVPPKVVNYE AGEWAENCYN LEIKKPDGSE 

       130        140        150        160        170        180 
CLPAAPDGIR GFPRCRYVHK VSGTGPCAGD FAFHKEGAFF LYDRLASTVI YRGTTFAEGV 

       190        200        210        220        230        240 
VAFLILPQAK KDFFSSHPLR EPVNATEDPS SGYYSTTIRY QATGFGTNET EYLFEVDNLT 

       250        260        270        280        290        300 
YVQLESRFTP QFLLQLNETI YTSGKRSNTT GKLIWKVNPE IDTTIGEWAF WETKKTSLEK 

       310        320        330        340        350        360 
FAVKSCLSQL YQTEPKTSVV RVRRELLPTQ GPTQQLKTTK SWLQKIPLQW FKCTVKEGKL 


QCRI

« Hide

References

[1]"GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases."
Volchkov V.E., Becker S., Volchkova V.A., Ternovoj V.A., Kotov A.N., Netesov S.V., Klenk H.-D.
Virology 214:421-430(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA / MRNA], RNA EDITING.
[2]"The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing."
Sanchez A., Trappier S.G., Mahy B.W.J., Peters C.J., Nichol S.T.
Proc. Natl. Acad. Sci. U.S.A. 93:3602-3607(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], RNA EDITING.
[3]"Molecular characterization of guinea pig-adapted variants of Ebola virus."
Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate guinea pig-adapted.
[4]Volchkov V.E.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[5]Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate mouse-adapted.
[6]"Delta-peptide is the carboxy-terminal cleavage fragment of the nonstructural small glycoprotein sGP of Ebola virus."
Volchkova V.A., Klenk H.-D., Volchkov V.E.
Virology 265:164-171(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF ARG-324.
[7]"Disulfide bond assignment of the Ebola virus secreted glycoprotein SGP."
Barrientos L.G., Martin A.M., Rollin P.E., Sanchez A.
Biochem. Biophys. Res. Commun. 323:696-702(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
[8]"Ebola virus glycoprotein: proteolytic processing, acylation, cell tropism, and detection of neutralizing antibodies."
Ito H., Watanabe S., Takada A., Kawaoka Y.
J. Virol. 75:1576-1580(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF SGP.
[9]"Evidence against Ebola virus sGP binding to human neutrophils by a specific receptor."
Sui J., Marasco W.A.
Virology 303:9-14(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF SGP.
[10]"Effects of Ebola virus glycoproteins on endothelial cell activation and barrier function."
Wahl-Jensen V.M., Afanasieva T.A., Seebach J., Stroeher U., Feldmann H., Schnittler H.J.
J. Virol. 79:10442-10450(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF SGP.
[11]"Role of Ebola virus secreted glycoproteins and virus-like particles in activation of human macrophages."
Wahl-Jensen V., Kurz S.K., Hazelton P.R., Schnittler H.J., Stroeher U., Burton D.R., Feldmann H.
J. Virol. 79:2413-2419(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U31033 Genomic RNA. Translation: AAA96745.1.
U23187 Genomic RNA. Translation: AAC54886.1.
AF272001 Genomic RNA. Translation: AAG40167.1.
AY142960 Genomic RNA. Translation: AAN37508.1.
AF086833 Genomic RNA. Translation: AAD14584.1.
AF499101 Genomic RNA. Translation: AAM76035.1.
RefSeqNP_066247.1. NC_002549.1.

3D structure databases

ProteinModelPortalP60170.
SMRP60170. Positions 32-281.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID911829.

Family and domain databases

InterProIPR014625. GPC_FiloV.
IPR002561. GPC_filovir-type_extra_dom.
[Graphical view]
PfamPF01611. Filo_glycop. 1 hit.
[Graphical view]
PIRSFPIRSF036874. GPC_FiloV. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVSGP_EBOZM
AccessionPrimary (citable) accession number: P60170
Secondary accession number(s): O12421 expand/collapse secondary AC list , O12717, Q66801, Q66819, Q77LU4, Q8JS61, Q9YMG3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents