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P60170

- VSGP_EBOZM

UniProt

P60170 - VSGP_EBOZM

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Protein
Pre-small/secreted glycoprotein
Gene
GP
Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils.4 Publications
Delta-peptide does not seem to be involved in activation of primary macrophages.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei324 – 3252Cleavage; by host furin

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-small/secreted glycoprotein
Short name:
pre-sGP
Cleaved into the following 2 chains:
Gene namesi
Name:GP
OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128952 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007209: Genome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi324 – 3241R → S: Loss of cleavage. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed prediction
Add
BLAST
Chaini33 – 364332Pre-small/secreted glycoprotein
PRO_0000037512Add
BLAST
Chaini33 – 324292Small/secreted glycoprotein
PRO_0000037513Add
BLAST
Chaini325 – 36440Delta-peptide
PRO_0000037514Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi53 – 53Interchain1 Publication
Disulfide bondi108 ↔ 1351 Publication
Disulfide bondi121 ↔ 1471 Publication
Glycosylationi204 – 2041N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi228 – 2281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi238 – 2381N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi257 – 2571N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi268 – 2681N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi306 – 306Interchain1 Publication

Post-translational modificationi

Pre-sGP is N-glycosylated. This precursor is processed into mature sGP and delta-peptide by host furin or furin-like proteases. The cleavage site corresponds to the furin optimal cleavage sequence [KR]-X-[KR]-R. Both cleavage fragments contain sialic acid, but only the delta-peptide is O-glycosylated.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

sGP is a homodimer; disulfide-linked. The homodimers are linked by two disulfide bonds in a parallel orientation. Delta-peptide is a monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP60170.
SMRiP60170. Positions 32-281.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR014625. GPC_FiloV.
IPR002561. GPC_filovir-type_extra_dom.
[Graphical view]
PfamiPF01611. Filo_glycop. 1 hit.
[Graphical view]
PIRSFiPIRSF036874. GPC_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P60170-1 [UniParc]FASTAAdd to Basket

« Hide

MGVTGILQLP RDRFKRTSFF LWVIILFQRT FSIPLGVIHN STLQVSDVDK    50
LVCRDKLSST NQLRSVGLNL EGNGVATDVP SATKRWGFRS GVPPKVVNYE 100
AGEWAENCYN LEIKKPDGSE CLPAAPDGIR GFPRCRYVHK VSGTGPCAGD 150
FAFHKEGAFF LYDRLASTVI YRGTTFAEGV VAFLILPQAK KDFFSSHPLR 200
EPVNATEDPS SGYYSTTIRY QATGFGTNET EYLFEVDNLT YVQLESRFTP 250
QFLLQLNETI YTSGKRSNTT GKLIWKVNPE IDTTIGEWAF WETKKTSLEK 300
FAVKSCLSQL YQTEPKTSVV RVRRELLPTQ GPTQQLKTTK SWLQKIPLQW 350
FKCTVKEGKL QCRI 364
Length:364
Mass (Da):41,175
Last modified:December 15, 2003 - v1
Checksum:i67376A454CE5F362
GO

RNA editingi

Partially edited. RNA editing at this position consists of an insertion of one or two adenine nucleotides. The sequence displayed here is the small secreted glycoprotein, derived from the unedited RNA. The sequence derived from the +1A edited gives rise to the full-length transmembrane glycoprotein GP (AC Q05320), the +2A edited RNA gives rise to the super small secreted glycoprotein ssGP (AC Q9YMG2).2 Publications

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651S → P in strain: Isolate mouse-adapted.
Natural varianti246 – 2461S → P in strain: Isolate mouse-adapted.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31033 Genomic RNA. Translation: AAA96745.1.
U23187 Genomic RNA. Translation: AAC54886.1.
AF272001 Genomic RNA. Translation: AAG40167.1.
AY142960 Genomic RNA. Translation: AAN37508.1.
AF086833 Genomic RNA. Translation: AAD14584.1.
AF499101 Genomic RNA. Translation: AAM76035.1.
RefSeqiNP_066247.1. NC_002549.1.

Genome annotation databases

GeneIDi911829.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31033 Genomic RNA. Translation: AAA96745.1 .
U23187 Genomic RNA. Translation: AAC54886.1 .
AF272001 Genomic RNA. Translation: AAG40167.1 .
AY142960 Genomic RNA. Translation: AAN37508.1 .
AF086833 Genomic RNA. Translation: AAD14584.1 .
AF499101 Genomic RNA. Translation: AAM76035.1 .
RefSeqi NP_066247.1. NC_002549.1.

3D structure databases

ProteinModelPortali P60170.
SMRi P60170. Positions 32-281.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911829.

Family and domain databases

InterProi IPR014625. GPC_FiloV.
IPR002561. GPC_filovir-type_extra_dom.
[Graphical view ]
Pfami PF01611. Filo_glycop. 1 hit.
[Graphical view ]
PIRSFi PIRSF036874. GPC_FiloV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases."
    Volchkov V.E., Becker S., Volchkova V.A., Ternovoj V.A., Kotov A.N., Netesov S.V., Klenk H.-D.
    Virology 214:421-430(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA / MRNA], RNA EDITING.
  2. "The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing."
    Sanchez A., Trappier S.G., Mahy B.W.J., Peters C.J., Nichol S.T.
    Proc. Natl. Acad. Sci. U.S.A. 93:3602-3607(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], RNA EDITING.
  3. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
    Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
    Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate guinea pig-adapted.
  4. Volchkov V.E.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate mouse-adapted.
  6. "Delta-peptide is the carboxy-terminal cleavage fragment of the nonstructural small glycoprotein sGP of Ebola virus."
    Volchkova V.A., Klenk H.-D., Volchkov V.E.
    Virology 265:164-171(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF ARG-324.
  7. "Disulfide bond assignment of the Ebola virus secreted glycoprotein SGP."
    Barrientos L.G., Martin A.M., Rollin P.E., Sanchez A.
    Biochem. Biophys. Res. Commun. 323:696-702(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.
  8. "Ebola virus glycoprotein: proteolytic processing, acylation, cell tropism, and detection of neutralizing antibodies."
    Ito H., Watanabe S., Takada A., Kawaoka Y.
    J. Virol. 75:1576-1580(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF SGP.
  9. "Evidence against Ebola virus sGP binding to human neutrophils by a specific receptor."
    Sui J., Marasco W.A.
    Virology 303:9-14(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF SGP.
  10. "Effects of Ebola virus glycoproteins on endothelial cell activation and barrier function."
    Wahl-Jensen V.M., Afanasieva T.A., Seebach J., Stroeher U., Feldmann H., Schnittler H.J.
    J. Virol. 79:10442-10450(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF SGP.
  11. "Role of Ebola virus secreted glycoproteins and virus-like particles in activation of human macrophages."
    Wahl-Jensen V., Kurz S.K., Hazelton P.R., Schnittler H.J., Stroeher U., Burton D.R., Feldmann H.
    J. Virol. 79:2413-2419(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVSGP_EBOZM
AccessioniPrimary (citable) accession number: P60170
Secondary accession number(s): O12421
, O12717, Q66801, Q66819, Q77LU4, Q8JS61, Q9YMG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: December 11, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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