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Protein

Inactive ribonuclease-like protein 9

Gene

RNASE9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Does not exhibit any ribonuclease activity.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive ribonuclease-like protein 9
Gene namesi
Name:RNASE9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20673. RNASE9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134887351.

Polymorphism and mutation databases

BioMutaiRNASE9.
DMDMi38605140.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 205179Inactive ribonuclease-like protein 9PRO_0000030951Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi116 ↔ 168By similarity
Disulfide bondi123 ↔ 130By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi143 – 1431N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP60153.
PRIDEiP60153.

Expressioni

Tissue specificityi

At the mRNA level, widely expressed (PubMed:18992174). At protein level, restricted to epididymis (PubMed:19137000). Expressed in spermatozoa (sperm head and neck), with higher levels on ejaculated and epididymal sperm than on testicular sperm (at protein level). Expressed in the epithelial cells of the epididymal tubule (at protein level). Not detected in muscle.2 Publications

Gene expression databases

BgeeiP60153.
CleanExiHS_RNASE9.
ExpressionAtlasiP60153. baseline.
GenevestigatoriP60153.

Organism-specific databases

HPAiHPA003391.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000340162.

Structurei

3D structure databases

ProteinModelPortaliP60153.
SMRiP60153. Positions 60-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG46399.
GeneTreeiENSGT00390000013952.
HOGENOMiHOG000236243.
HOVERGENiHBG056874.
InParanoidiP60153.
OMAiKNGIRKC.
OrthoDBiEOG7XDBHR.
PhylomeDBiP60153.
TreeFamiTF338401.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR029741. RNASE9.
IPR001427. RNaseA.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PTHR11437:SF14. PTHR11437:SF14. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54076. SSF54076. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60153-1) [UniParc]FASTAAdd to basket

Also known as: alpha1, alpha2, alpha12, beta2, beta12

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRTLITTHP LPLLLLPQQL LQLVQFQEVD TDFDFPEEDK KEEFEECLEK
60 70 80 90 100
FFSTGPARPP TKEKVKRRVL IEPGMPLNHI EYCNHEIMGK NVYYKHRWVA
110 120 130 140 150
EHYFLLMQYD ELQKICYNRF VPCKNGIRKC NRSKGLVEGV YCNLTEAFEI
160 170 180 190 200
PACKYESLYR KGYVLITCSW QNEMQKRIPH TINDLVEPPE HRSFLSEDGV

FVISP
Length:205
Mass (Da):24,307
Last modified:November 28, 2003 - v1
Checksum:iEDE63F0727282BF6
GO
Isoform 2 (identifier: P60153-2) [UniParc]FASTAAdd to basket

Also known as: alpha23, alpha123

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSAGKM

Show »
Length:210
Mass (Da):24,782
Checksum:iFCF929B3261222E6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti148 – 1481F → S.3 Publications
Corresponds to variant rs12590446 [ dbSNP | Ensembl ].
VAR_052197
Natural varianti204 – 2041S → P.3 Publications
Corresponds to variant rs1243647 [ dbSNP | Ensembl ].
VAR_034473

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MSAGKM in isoform 2. 1 PublicationVSP_041856

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665804 mRNA. Translation: AAV87182.1.
EU414264 mRNA. Translation: ACA49544.1.
AF382949 mRNA. Translation: AAQ02792.1.
AY907670 mRNA. Translation: AAX86045.1.
EF061296 mRNA. Translation: ABP97451.1.
EF061297 mRNA. Translation: ABP97452.1.
EF061298 mRNA. Translation: ABP97453.1.
EF061299 mRNA. Translation: ABP97454.1.
EF061300 mRNA. Translation: ABP97455.1.
EF061301 mRNA. Translation: ABP97456.1.
EF061302 mRNA. Translation: ABP97457.1.
EF061303 mRNA. Translation: ABP97458.1.
EF061304 mRNA. Translation: ABP97459.1.
AL163195 Genomic DNA. No translation available.
BC130311 mRNA. Translation: AAI30312.1.
BC130313 mRNA. Translation: AAI30314.1.
CCDSiCCDS32036.1. [P60153-1]
CCDS53883.1. [P60153-2]
CCDS55904.1. [P60153-2]
RefSeqiNP_001001673.2. NM_001001673.3. [P60153-1]
NP_001103826.1. NM_001110356.1. [P60153-1]
NP_001103827.1. NM_001110357.1. [P60153-1]
NP_001103828.1. NM_001110358.1. [P60153-2]
NP_001103829.1. NM_001110359.1. [P60153-2]
NP_001103830.1. NM_001110360.1. [P60153-2]
NP_001103831.1. NM_001110361.1. [P60153-2]
NP_001276039.1. NM_001289110.1. [P60153-1]
UniGeneiHs.533972.

Genome annotation databases

EnsembliENST00000338904; ENSP00000340162; ENSG00000188655. [P60153-1]
ENST00000404716; ENSP00000384683; ENSG00000188655. [P60153-2]
ENST00000429244; ENSP00000409504; ENSG00000188655. [P60153-1]
ENST00000553541; ENSP00000451285; ENSG00000188655. [P60153-1]
ENST00000553706; ENSP00000450570; ENSG00000188655. [P60153-2]
ENST00000554964; ENSP00000450599; ENSG00000188655. [P60153-1]
ENST00000555230; ENSP00000450800; ENSG00000188655. [P60153-1]
ENST00000556208; ENSP00000451160; ENSG00000188655. [P60153-2]
ENST00000557068; ENSP00000451565; ENSG00000188655. [P60153-1]
ENST00000557209; ENSP00000450987; ENSG00000188655. [P60153-2]
ENST00000611135; ENSP00000481786; ENSG00000188655. [P60153-2]
GeneIDi390443.
KEGGihsa:390443.
UCSCiuc001vxq.4. human. [P60153-2]
uc010aho.3. human. [P60153-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665804 mRNA. Translation: AAV87182.1.
EU414264 mRNA. Translation: ACA49544.1.
AF382949 mRNA. Translation: AAQ02792.1.
AY907670 mRNA. Translation: AAX86045.1.
EF061296 mRNA. Translation: ABP97451.1.
EF061297 mRNA. Translation: ABP97452.1.
EF061298 mRNA. Translation: ABP97453.1.
EF061299 mRNA. Translation: ABP97454.1.
EF061300 mRNA. Translation: ABP97455.1.
EF061301 mRNA. Translation: ABP97456.1.
EF061302 mRNA. Translation: ABP97457.1.
EF061303 mRNA. Translation: ABP97458.1.
EF061304 mRNA. Translation: ABP97459.1.
AL163195 Genomic DNA. No translation available.
BC130311 mRNA. Translation: AAI30312.1.
BC130313 mRNA. Translation: AAI30314.1.
CCDSiCCDS32036.1. [P60153-1]
CCDS53883.1. [P60153-2]
CCDS55904.1. [P60153-2]
RefSeqiNP_001001673.2. NM_001001673.3. [P60153-1]
NP_001103826.1. NM_001110356.1. [P60153-1]
NP_001103827.1. NM_001110357.1. [P60153-1]
NP_001103828.1. NM_001110358.1. [P60153-2]
NP_001103829.1. NM_001110359.1. [P60153-2]
NP_001103830.1. NM_001110360.1. [P60153-2]
NP_001103831.1. NM_001110361.1. [P60153-2]
NP_001276039.1. NM_001289110.1. [P60153-1]
UniGeneiHs.533972.

3D structure databases

ProteinModelPortaliP60153.
SMRiP60153. Positions 60-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000340162.

Polymorphism and mutation databases

BioMutaiRNASE9.
DMDMi38605140.

Proteomic databases

PaxDbiP60153.
PRIDEiP60153.

Protocols and materials databases

DNASUi390443.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338904; ENSP00000340162; ENSG00000188655. [P60153-1]
ENST00000404716; ENSP00000384683; ENSG00000188655. [P60153-2]
ENST00000429244; ENSP00000409504; ENSG00000188655. [P60153-1]
ENST00000553541; ENSP00000451285; ENSG00000188655. [P60153-1]
ENST00000553706; ENSP00000450570; ENSG00000188655. [P60153-2]
ENST00000554964; ENSP00000450599; ENSG00000188655. [P60153-1]
ENST00000555230; ENSP00000450800; ENSG00000188655. [P60153-1]
ENST00000556208; ENSP00000451160; ENSG00000188655. [P60153-2]
ENST00000557068; ENSP00000451565; ENSG00000188655. [P60153-1]
ENST00000557209; ENSP00000450987; ENSG00000188655. [P60153-2]
ENST00000611135; ENSP00000481786; ENSG00000188655. [P60153-2]
GeneIDi390443.
KEGGihsa:390443.
UCSCiuc001vxq.4. human. [P60153-2]
uc010aho.3. human. [P60153-1]

Organism-specific databases

CTDi390443.
GeneCardsiGC14M021024.
HGNCiHGNC:20673. RNASE9.
HPAiHPA003391.
MIMi614014. gene.
neXtProtiNX_P60153.
PharmGKBiPA134887351.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG46399.
GeneTreeiENSGT00390000013952.
HOGENOMiHOG000236243.
HOVERGENiHBG056874.
InParanoidiP60153.
OMAiKNGIRKC.
OrthoDBiEOG7XDBHR.
PhylomeDBiP60153.
TreeFamiTF338401.

Miscellaneous databases

GenomeRNAii390443.
NextBioi103822.
PROiP60153.
SOURCEiSearch...

Gene expression databases

BgeeiP60153.
CleanExiHS_RNASE9.
ExpressionAtlasiP60153. baseline.
GenevestigatoriP60153.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR029741. RNASE9.
IPR001427. RNaseA.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PTHR11437:SF14. PTHR11437:SF14. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54076. SSF54076. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ribonuclease A superfamily of mammals and birds: identifying new members and tracing evolutionary histories."
    Cho S., Beintema J.J., Zhang J.
    Genomics 85:208-220(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Cloning, expression and location of RNase9 in human epididymis."
    Liu J., Li J., Wang H., Zhang C., Li N., Lin Y., Liu J., Wang W.
    BMC Res. Notes 1:111-111(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-148 AND PRO-204, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Epididymis.
  3. "Human RNASE9 transcript variants."
    Liu Q., Hamil K.G., Gao B., French F.S., Hall S.H., Zhang Y.-L.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS SER-148 AND PRO-204.
  4. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-148 AND PRO-204.
  6. "Human ribonuclease 9, a member of ribonuclease A superfamily, specifically expressed in epididymis, is a novel sperm-binding protein."
    Cheng G.Z., Li J.Y., Li F., Wang H.Y., Shi G.X.
    Asian J. Androl. 11:240-251(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-36, FUNCTION, TISSUE SPECIFICITY, SIGNAL SEQUENCE CLEAVAGE SITE.

Entry informationi

Entry nameiRNAS9_HUMAN
AccessioniPrimary (citable) accession number: P60153
Secondary accession number(s): A2RQR8
, A8QJS1, Q5GAN7, Q6KG53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: May 27, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.