P60153 (RNAS9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inactive ribonuclease-like protein 9

Gene names

Name:

RNASE9

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Does not exhibit any ribonuclease activity. Ref.2 Ref.6
Subcellular location	Secreted Probable.
Tissue specificity	At the mRNA level, widely expressed (Ref.2). At protein level, restricted to epididymis (Ref.6). Expressed in spermatozoa (sperm head and neck), with higher levels on ejaculated and epididymal sperm than on testicular sperm (at protein level). Expressed in the epithelial cells of the epididymal tubule (at protein level). Not detected in muscle. Ref.2 Ref.6
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P60153-1)

Also known as: alpha1; alpha2; alpha12; beta2; beta12;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P60153-2)

Also known as: alpha23; alpha123;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSAGKM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.6
	Chain	27 – 205	179	Inactive ribonuclease-like protein 9		PRO_0000030951
Amino acid modifications
	Glycosylation	131	1	N-linked (GlcNAc...) Potential
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 168		By similarity
	Disulfide bond	123 ↔ 130		By similarity
Natural variations
	Alternative sequence	1	1	M → MSAGKM in isoform 2.		VSP_041856
	Natural variant	148	1	F → S. Ref.2 Ref.3 Ref.5 Corresponds to variant rs12590446 [ dbSNP | Ensembl ].		VAR_052197
	Natural variant	204	1	S → P. Ref.2 Ref.3 Ref.5 Corresponds to variant rs1243647 [ dbSNP | Ensembl ].		VAR_034473

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (alpha1) (alpha2) (alpha12) (beta2) (beta12) [UniParc]. Last modified November 28, 2003. Version 1. Checksum: EDE63F0727282BF6	FASTA	205	24,307
	10 20 30 40 50 60 MMRTLITTHP LPLLLLPQQL LQLVQFQEVD TDFDFPEEDK KEEFEECLEK FFSTGPARPP 70 80 90 100 110 120 TKEKVKRRVL IEPGMPLNHI EYCNHEIMGK NVYYKHRWVA EHYFLLMQYD ELQKICYNRF 130 140 150 160 170 180 VPCKNGIRKC NRSKGLVEGV YCNLTEAFEI PACKYESLYR KGYVLITCSW QNEMQKRIPH 190 200 TINDLVEPPE HRSFLSEDGV FVISP
	Isoform 2 (alpha23) (alpha123) [UniParc]. Checksum: FCF929B3261222E6	FASTA	210	24,782
	10 20 30 40 50 60 MSAGKMMRTL ITTHPLPLLL LPQQLLQLVQ FQEVDTDFDF PEEDKKEEFE ECLEKFFSTG 70 80 90 100 110 120 PARPPTKEKV KRRVLIEPGM PLNHIEYCNH EIMGKNVYYK HRWVAEHYFL LMQYDELQKI 130 140 150 160 170 180 CYNRFVPCKN GIRKCNRSKG LVEGVYCNLT EAFEIPACKY ESLYRKGYVL ITCSWQNEMQ 190 200 210 KRIPHTINDL VEPPEHRSFL SEDGVFVISP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ribonuclease A superfamily of mammals and birds: identifying new members and tracing evolutionary histories." Cho S., Beintema J.J., Zhang J. Genomics 85:208-220(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Cloning, expression and location of RNase9 in human epididymis." Liu J., Li J., Wang H., Zhang C., Li N., Lin Y., Liu J., Wang W. BMC Res. Notes 1:111-111(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-148 AND PRO-204, FUNCTION, TISSUE SPECIFICITY. Tissue: Epididymis.
[3]	"Human RNASE9 transcript variants." Liu Q., Hamil K.G., Gao B., French F.S., Hall S.H., Zhang Y.-L. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS SER-148 AND PRO-204.
[4]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-148 AND PRO-204.
[6]	"Human ribonuclease 9, a member of ribonuclease A superfamily, specifically expressed in epididymis, is a novel sperm-binding protein." Cheng G.Z., Li J.Y., Li F., Wang H.Y., Shi G.X. Asian J. Androl. 11:240-251(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-36, FUNCTION, TISSUE SPECIFICITY, SIGNAL SEQUENCE CLEAVAGE SITE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY665804 mRNA. Translation: AAV87182.1. EU414264 mRNA. Translation: ACA49544.1. AF382949 mRNA. Translation: AAQ02792.1. AY907670 mRNA. Translation: AAX86045.1. EF061296 mRNA. Translation: ABP97451.1. EF061297 mRNA. Translation: ABP97452.1. EF061298 mRNA. Translation: ABP97453.1. EF061299 mRNA. Translation: ABP97454.1. EF061300 mRNA. Translation: ABP97455.1. EF061301 mRNA. Translation: ABP97456.1. EF061302 mRNA. Translation: ABP97457.1. EF061303 mRNA. Translation: ABP97458.1. EF061304 mRNA. Translation: ABP97459.1. AL163195 Genomic DNA. No translation available. BC130311 mRNA. Translation: AAI30312.1. BC130313 mRNA. Translation: AAI30314.1.
IPI	IPI00375936.
RefSeq	NP_001001673.2. NM_001001673.3. NP_001103826.1. NM_001110356.1. NP_001103827.1. NM_001110357.1. NP_001103828.1. NM_001110358.1. NP_001103829.1. NM_001110359.1. NP_001103830.1. NM_001110360.1. NP_001103831.1. NM_001110361.1.
UniGene	Hs.533972.
3D structure databases
ProteinModelPortal	P60153.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000340162.
Polymorphism databases
DMDM	38605140.
Proteomic databases
PaxDb	P60153.
PRIDE	P60153.
Protocols and materials databases
DNASU	390443.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338904; ENSP00000340162; ENSG00000188655. ENST00000404716; ENSP00000384683; ENSG00000188655. ENST00000429244; ENSP00000409504; ENSG00000188655. ENST00000553541; ENSP00000451285; ENSG00000188655. ENST00000553706; ENSP00000450570; ENSG00000188655. ENST00000554964; ENSP00000450599; ENSG00000188655. ENST00000555230; ENSP00000450800; ENSG00000188655. ENST00000556208; ENSP00000451160; ENSG00000188655. ENST00000557068; ENSP00000451565; ENSG00000188655. ENST00000557209; ENSP00000450987; ENSG00000188655.
GeneID	390443.
KEGG	hsa:390443.
UCSC	uc010aho.3. human.
Organism-specific databases
CTD	390443.
GeneCards	GC14M021024.
HGNC	HGNC:20673. RNASE9.
HPA	HPA003391.
MIM	614014. gene.
neXtProt	NX_P60153.
PharmGKB	PA134887351.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46399.
HOGENOM	HOG000236243.
HOVERGEN	HBG056874.
InParanoid	P60153.
OMA	CKNGIRK.
OrthoDB	EOG46WZ9K.
PhylomeDB	P60153.
Gene expression databases
Bgee	P60153.
CleanEx	HS_RNASE9.
Genevestigator	P60153.
Family and domain databases
Gene3D	3.10.130.10. 1 hit.
InterPro	IPR001427. RNaseA. IPR023412. RNaseA_domain. [Graphical view]
PANTHER	PTHR11437. PTHR11437. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54076. RNaseA. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	390443.
NextBio	103822.
SOURCE	Search...

Entry information

Entry name

RNAS9_HUMAN

Accession

Primary (citable) accession number: P60153
Secondary accession number(s): A2RQR8 , A8QJS1, Q5GAN7, Q6KG53

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.