Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P60153 (RNAS9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inactive ribonuclease-like protein 9
Gene names
Name:RNASE9
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Does not exhibit any ribonuclease activity. Ref.2 Ref.6

Subcellular location

Secreted Probable.

Tissue specificity

At the mRNA level, widely expressed (Ref.2). At protein level, restricted to epididymis (Ref.6). Expressed in spermatozoa (sperm head and neck), with higher levels on ejaculated and epididymal sperm than on testicular sperm (at protein level). Expressed in the epithelial cells of the epididymal tubule (at protein level). Not detected in muscle. Ref.2 Ref.6

Sequence similarities

Belongs to the pancreatic ribonuclease family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P60153-1)

Also known as: alpha1; alpha2; alpha12; beta2; beta12;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P60153-2)

Also known as: alpha23; alpha123;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSAGKM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.6
Chain27 – 205179Inactive ribonuclease-like protein 9
PRO_0000030951

Amino acid modifications

Glycosylation1311N-linked (GlcNAc...) Potential
Glycosylation1431N-linked (GlcNAc...) Potential
Disulfide bond116 ↔ 168 By similarity
Disulfide bond123 ↔ 130 By similarity

Natural variations

Alternative sequence11M → MSAGKM in isoform 2.
VSP_041856
Natural variant1481F → S. Ref.2 Ref.3 Ref.5
Corresponds to variant rs12590446 [ dbSNP | Ensembl ].
VAR_052197
Natural variant2041S → P. Ref.2 Ref.3 Ref.5
Corresponds to variant rs1243647 [ dbSNP | Ensembl ].
VAR_034473

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (alpha1) (alpha2) (alpha12) (beta2) (beta12) [UniParc].

Last modified November 28, 2003. Version 1.
Checksum: EDE63F0727282BF6

FASTA20524,307
        10         20         30         40         50         60 
MMRTLITTHP LPLLLLPQQL LQLVQFQEVD TDFDFPEEDK KEEFEECLEK FFSTGPARPP 

        70         80         90        100        110        120 
TKEKVKRRVL IEPGMPLNHI EYCNHEIMGK NVYYKHRWVA EHYFLLMQYD ELQKICYNRF 

       130        140        150        160        170        180 
VPCKNGIRKC NRSKGLVEGV YCNLTEAFEI PACKYESLYR KGYVLITCSW QNEMQKRIPH 

       190        200 
TINDLVEPPE HRSFLSEDGV FVISP 

« Hide

Isoform 2 (alpha23) (alpha123) [UniParc].

Checksum: FCF929B3261222E6
Show »

FASTA21024,782

References

« Hide 'large scale' references
[1]"The ribonuclease A superfamily of mammals and birds: identifying new members and tracing evolutionary histories."
Cho S., Beintema J.J., Zhang J.
Genomics 85:208-220(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning, expression and location of RNase9 in human epididymis."
Liu J., Li J., Wang H., Zhang C., Li N., Lin Y., Liu J., Wang W.
BMC Res. Notes 1:111-111(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS SER-148 AND PRO-204, FUNCTION, TISSUE SPECIFICITY.
Tissue: Epididymis.
[3]"Human RNASE9 transcript variants."
Liu Q., Hamil K.G., Gao B., French F.S., Hall S.H., Zhang Y.-L.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANTS SER-148 AND PRO-204.
[4]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-148 AND PRO-204.
[6]"Human ribonuclease 9, a member of ribonuclease A superfamily, specifically expressed in epididymis, is a novel sperm-binding protein."
Cheng G.Z., Li J.Y., Li F., Wang H.Y., Shi G.X.
Asian J. Androl. 11:240-251(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-36, FUNCTION, TISSUE SPECIFICITY, SIGNAL SEQUENCE CLEAVAGE SITE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY665804 mRNA. Translation: AAV87182.1.
EU414264 mRNA. Translation: ACA49544.1.
AF382949 mRNA. Translation: AAQ02792.1.
AY907670 mRNA. Translation: AAX86045.1.
EF061296 mRNA. Translation: ABP97451.1.
EF061297 mRNA. Translation: ABP97452.1.
EF061298 mRNA. Translation: ABP97453.1.
EF061299 mRNA. Translation: ABP97454.1.
EF061300 mRNA. Translation: ABP97455.1.
EF061301 mRNA. Translation: ABP97456.1.
EF061302 mRNA. Translation: ABP97457.1.
EF061303 mRNA. Translation: ABP97458.1.
EF061304 mRNA. Translation: ABP97459.1.
AL163195 Genomic DNA. No translation available.
BC130311 mRNA. Translation: AAI30312.1.
BC130313 mRNA. Translation: AAI30314.1.
CCDSCCDS32036.1. [P60153-1]
CCDS53883.1. [P60153-2]
CCDS55904.1. [P60153-2]
RefSeqNP_001001673.2. NM_001001673.3. [P60153-1]
NP_001103826.1. NM_001110356.1. [P60153-1]
NP_001103827.1. NM_001110357.1. [P60153-1]
NP_001103828.1. NM_001110358.1. [P60153-2]
NP_001103829.1. NM_001110359.1. [P60153-2]
NP_001103830.1. NM_001110360.1. [P60153-2]
NP_001103831.1. NM_001110361.1. [P60153-2]
NP_001276039.1. NM_001289110.1. [P60153-1]
XP_006720205.1. XM_006720142.1. [P60153-1]
UniGeneHs.533972.

3D structure databases

ProteinModelPortalP60153.
SMRP60153. Positions 80-186.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000340162.

Polymorphism databases

DMDM38605140.

Proteomic databases

PaxDbP60153.
PRIDEP60153.

Protocols and materials databases

DNASU390443.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000338904; ENSP00000340162; ENSG00000188655. [P60153-1]
ENST00000404716; ENSP00000384683; ENSG00000188655. [P60153-2]
ENST00000429244; ENSP00000409504; ENSG00000188655. [P60153-1]
ENST00000553541; ENSP00000451285; ENSG00000188655. [P60153-1]
ENST00000553706; ENSP00000450570; ENSG00000188655. [P60153-2]
ENST00000554964; ENSP00000450599; ENSG00000188655. [P60153-1]
ENST00000555230; ENSP00000450800; ENSG00000188655. [P60153-1]
ENST00000556208; ENSP00000451160; ENSG00000188655. [P60153-2]
ENST00000557068; ENSP00000451565; ENSG00000188655. [P60153-1]
ENST00000557209; ENSP00000450987; ENSG00000188655. [P60153-2]
GeneID390443.
KEGGhsa:390443.
UCSCuc001vxq.4. human. [P60153-2]
uc010aho.3. human. [P60153-1]

Organism-specific databases

CTD390443.
GeneCardsGC14M021024.
HGNCHGNC:20673. RNASE9.
HPAHPA003391.
MIM614014. gene.
neXtProtNX_P60153.
PharmGKBPA134887351.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG46399.
HOGENOMHOG000236243.
HOVERGENHBG056874.
InParanoidP60153.
OMAKNGIRKC.
OrthoDBEOG7XDBHR.
PhylomeDBP60153.
TreeFamTF338401.

Gene expression databases

BgeeP60153.
CleanExHS_RNASE9.
GenevestigatorP60153.

Family and domain databases

Gene3D3.10.130.10. 1 hit.
InterProIPR001427. RNaseA.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERPTHR11437. PTHR11437. 1 hit.
PfamPF00074. RnaseA. 1 hit.
[Graphical view]
ProDomPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF54076. SSF54076. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi390443.
NextBio103822.
PROP60153.
SOURCESearch...

Entry information

Entry nameRNAS9_HUMAN
AccessionPrimary (citable) accession number: P60153
Secondary accession number(s): A2RQR8 expand/collapse secondary AC list , A8QJS1, Q5GAN7, Q6KG53
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM