P60123 (RUVB1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RuvB-like 1
EC=3.6.4.12

Alternative name(s):
49 kDa TATA box-binding protein-interacting protein
Short name=49 kDa TBP-interacting protein
DNA helicase p50
Pontin 52
TIP49a

Gene names

Name:	Ruvbl1
Synonyms:	Tip49, Tip49a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity By similarity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage By similarity. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair By similarity. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation By similarity. May be able to bind plasminogen at cell surface and enhance plasminogen activation By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Forms homohexameric rings. Can form a dodecamer with RUVBL2 made of two stacked hexameric rings; however, even though RUVBL1 and RUVBL2 are present in equimolar ratio, the oligomeric status of each hexamer is not known. Oligomerization may regulate binding to nucleic acids and conversely, binding to nucleic acids may affect the dodecameric assembly. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex; specifically part of a complex module associated with the helicase ATP-binding and the helicase C-terminal domain of INO80. Interacts with IGHMBP2. Interacts with OFD1 By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Abundantly expressed in testes and moderately in spleen, thymus, and lung. In mouse seminiferous tubules, is specifically localized in germ cells from late pachytene spermatocytes to round spermatids. Ref.4
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair Growth regulation Mitosis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Chromatin regulator Helicase Hydrolase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW histone H2A acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4 acetylation Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	MLL1 complex Inferred from sequence or structural similarity. Source: UniProtKB NuA4 histone acetyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	RuvB-like 1		PRO_0000165641
Regions
	Nucleotide binding	70 – 77	8	ATP
Amino acid modifications
	Modified residue	2	1	N6-acetyllysine By similarity
	Modified residue	453	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60123 [UniParc]. Last modified November 28, 2003. Version 1. Checksum: 9AE73EFFBA194BA1	FASTA	456	50,214
	10 20 30 40 50 60 MKIEEVKSTT KTQRIASHSH VKGLGLDESG LAKQAASGLV GQENAREACG VIVELIKSKK 70 80 90 100 110 120 MAGRAVLLAG PPGTGKTALA LAIAQELGSK VPFCPMVGSE VYSTEIKKTE VLMENFRRAI 130 140 150 160 170 180 GLRIKETKEV YEGEVTELTP CETENPMGGY GKTISHVIIG LKTAKGTKQL KLDPSIFESL 190 200 210 220 230 240 QKERVEAGDV IYIEANSGAV KRQGRCDTYA TEFDLEAEEY VPLPKGDVHK KKEIIQDVTL 250 260 270 280 290 300 HDLDVANARP QGGQDILSMM GQLMKPKKTE ITDKLRGEIN KVVNKYIDQG VAELVPGVLF 310 320 330 340 350 360 VDEVHMLDIE CFTYLHRALE SSIAPIVIFA SNRGNCVIRG TEDITSPHGI PLDLLDRVMI 370 380 390 400 410 420 IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN 430 440 450 GKDSIEKEHV EEISELFYDA KSSAKILADQ QDKYMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB." Kanemaki M., Makino Y., Yoshida T., Kishimoto T., Koga A., Yamamoto K., Yamamoto M., Moncollin V., Egly J.-M., Muramatsu M., Tamura T.-A. Biochem. Biophys. Res. Commun. 235:64-68(1997) [PubMed: 9196036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-11; 34-45; 129-138 AND 446-452. Tissue: Liver.
[2]	"Molecular shape and ATP binding activity of rat p50, a putative mammalian homologue of RuvB DNA helicase." Kikuchi N., Gohshi T., Kawahire S., Tachibana T., Yoneda Y., Isobe T., Lim C.R., Kohno K., Ichimura T., Omata S., Horigome T. J. Biochem. 125:487-494(1999) [PubMed: 10050036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-9; 91-102; 377-398 AND 441. Strain: Fischer 344. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart and Ovary.
[4]	"A rat RuvB-like protein, TIP49a, is a germ cell-enriched novel DNA helicase." Makino Y., Kanemaki M., Kurokawa Y., Koji T., Tamura T.-A. J. Biol. Chem. 274:15329-15335(1999) [PubMed: 10336418] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB002406 mRNA. Translation: BAA20875.1. AB001581 mRNA. Translation: BAA76313.1. BC072511 mRNA. Translation: AAH72511.1. BC086531 mRNA. Translation: AAH86531.1.
IPI	IPI00212268.
PIR	JC5521.
RefSeq	NP_671706.1. NM_147177.1.
UniGene	Rn.86410.
3D structure databases
ProteinModelPortal	P60123.
SMR	P60123. Positions 7-453.
ModBase	Search...
Protein-protein interaction databases
IntAct	P60123. 1 interaction.
MINT	MINT-4133484.
STRING	P60123.
2D gel databases
World-2DPAGE	0004:P60123.
Proteomic databases
PRIDE	P60123.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018339; ENSRNOP00000018339; ENSRNOG00000013195.
GeneID	65137.
KEGG	rno:65137.
UCSC	NM_147177. rat.
Organism-specific databases
CTD	8607.
RGD	68373. Ruvbl1.
Phylogenomic databases
eggNOG	maNOG08882.
GeneTree	ENSGT00550000075043.
HOVERGEN	HBG054186.
InParanoid	P60123.
OMA	VHKKKEV.
OrthoDB	EOG46Q6SH.
PhylomeDB	P60123.
Gene expression databases
ArrayExpress	P60123.
Genevestigator	P60123.
GermOnline	ENSRNOG00000013195. Rattus norvegicus.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR016027. NA-bd_OB-fold-like. IPR010339. TIP49_C. [Graphical view]
KO	K04499.
Pfam	PF06068. TIP49. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...
Other
NextBio	613955.

Entry information

Entry name

RUVB1_RAT

Accession

Primary (citable) accession number: P60123
Secondary accession number(s): O35753

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program