Reviewed, UniProtKB/Swiss-Prot P60123 (RUVB1_RAT)

Names and origin

Protein names

Recommended name:
    RuvB-like 1
    EC=3.6.1.-
Alternative name(s):
    49 kDa TATA box-binding protein-interacting protein
      Short name=49 kDa TBP-interacting protein
    TIP49a
    Pontin 52
    DNA helicase p50

Gene names

Name:	Ruvbl1
Synonyms:	Tip49, Tip49a

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex By similarity. May be able to bind plasminogen at cell surface and enhance plasminogen activation By similarity. Essential for cell proliferation By similarity.
Subunit structure	Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E By similarity. Interacts with IGHMBP2 By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Abundantly expressed in testes and moderately in spleen, thymus, and lung. In mouse seminiferous tubules, is specifically localized in germ cells from late pachytene spermatocytes to round spermatids. Ref.4
Sequence similarities	Belongs to the ruvB family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA recombination Growth regulation Mitosis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Chromatin regulator Helicase Hydrolase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW histone H2A acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4 acetylation Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	NuA4 histone acetyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	RuvB-like 1		PRO_0000165641
Regions
	Nucleotide binding	70 – 77	8	ATP
Amino acid modifications
	Modified residue	2	1	N6-acetyllysine By similarity
	Modified residue	453	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60123-1 [UniParc]. Last modified November 28, 2003. Version 1. Checksum: 9AE73EFFBA194BA1	FASTA	456	50,214
	10 20 30 40 50 60 MKIEEVKSTT KTQRIASHSH VKGLGLDESG LAKQAASGLV GQENAREACG VIVELIKSKK 70 80 90 100 110 120 MAGRAVLLAG PPGTGKTALA LAIAQELGSK VPFCPMVGSE VYSTEIKKTE VLMENFRRAI 130 140 150 160 170 180 GLRIKETKEV YEGEVTELTP CETENPMGGY GKTISHVIIG LKTAKGTKQL KLDPSIFESL 190 200 210 220 230 240 QKERVEAGDV IYIEANSGAV KRQGRCDTYA TEFDLEAEEY VPLPKGDVHK KKEIIQDVTL 250 260 270 280 290 300 HDLDVANARP QGGQDILSMM GQLMKPKKTE ITDKLRGEIN KVVNKYIDQG VAELVPGVLF 310 320 330 340 350 360 VDEVHMLDIE CFTYLHRALE SSIAPIVIFA SNRGNCVIRG TEDITSPHGI PLDLLDRVMI 370 380 390 400 410 420 IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN 430 440 450 GKDSIEKEHV EEISELFYDA KSSAKILADQ QDKYMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a rat 49-kDa TBP-interacting protein (TIP49) that is highly homologous to the bacterial RuvB." Kanemaki M., Makino Y., Yoshida T., Kishimoto T., Koga A., Yamamoto K., Yamamoto M., Moncollin V., Egly J.-M., Muramatsu M., Tamura T.-A. Biochem. Biophys. Res. Commun. 235:64-68(1997) [PubMed: 9196036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-11; 34-45; 129-138 AND 446-452. Tissue: Liver.
[2]	"Molecular shape and ATP binding activity of rat p50, a putative mammalian homologue of RuvB DNA helicase." Kikuchi N., Gohshi T., Kawahire S., Tachibana T., Yoneda Y., Isobe T., Lim C.R., Kohno K., Ichimura T., Omata S., Horigome T. J. Biochem. 125:487-494(1999) [PubMed: 10050036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-9; 91-102; 377-398 AND 441. Strain: Fischer 344. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart and Ovary.
[4]	"A rat RuvB-like protein, TIP49a, is a germ cell-enriched novel DNA helicase." Makino Y., Kanemaki M., Kurokawa Y., Koji T., Tamura T.-A. J. Biol. Chem. 274:15329-15335(1999) [PubMed: 10336418] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB002406 mRNA. Translation: BAA20875.1. AB001581 mRNA. Translation: BAA76313.1. BC072511 mRNA. Translation: AAH72511.1. BC086531 mRNA. Translation: AAH86531.1.
IPI	IPI00212268.
PIR	JC5521.
RefSeq	NP_671706.1.
UniGene	Rn.86410
3D structure databases
SMR	P60123. Positions 9-449.
ModBase	Search...
Protein-protein interaction databases
STRING	P60123.
Proteomic databases
PRIDE	P60123.
Genome annotation databases
Ensembl	ENSRNOT00000018339; ENSRNOP00000018339; ENSRNOG00000013195; Rattus norvegicus. [Genome view]
GeneID	65137.
KEGG	rno:65137.
UCSC	NM_147177. rat.
Organism-specific databases
CTD	65137.
RGD	68373. Ruvbl1.
Phylogenomic databases
HOVERGEN	P60123.
OMA	KRVGRSD.
Gene expression databases
ArrayExpress	P60123.
Genevestigator	P60123.
GermOnline	ENSRNOG00000013195. Rattus norvegicus.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR010339. TIP49_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF06068. TIP49. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613955.

Entry information

Entry name

RUVB1_RAT

Accession

Primary (citable) accession number: P60123
Secondary accession number(s): O35753

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)