Reviewed, UniProtKB/Swiss-Prot P60116 (ATP9_TOBAC)

Names and origin

Protein names

Recommended name:
    ATP synthase subunit 9, mitochondrial
    EC=3.6.3.14
Alternative name(s):
    Lipid-binding protein

Gene names

Name:

ATP9

Encoded on

Mitochondrion

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	74 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein is one of the chains of the nonenzymatic membrane component (F0) of mitochondrial ATPase.
Catalytic activity	ATP + H2O + H+(In) = ADP + phosphate + H+(Out).
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ATPase C chain family.
RNA editing	Edited at positions 7, 17, 28, 31, 61, 64, 71 and 75. The stop codon at position 75 is created by RNA editing. Ref.2

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion
Coding sequence diversity	RNA editing
Domain	Transmembrane
Ligand	ATP-binding Lipid-binding Nucleotide-binding
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW proton-transporting ATP synthase complex, coupling factor F(o) Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 74	74	ATP synthase subunit 9, mitochondrial		PRO_0000112225
Regions
	Transmembrane	8 – 28	21	Potential
	Transmembrane	50 – 70	21	Potential
Sites
	Site	57	1	Reversibly protonated during proton transport By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60116-1 [UniParc]. Last modified November 28, 2003. Version 1. Checksum: 6593295037555E82	FASTA	74	7,563
	10 20 30 40 50 60 MLEGAKLMGA GAATIALAGA AIGIGNVFSS LIHSVARNPS LAKQLFGYAI LGFALTEAIA 70 LFALMMAFLI SFVF

References

[1]	"The tobacco mitochondrial ATPase subunit 9 gene is closely linked to an open reading frame for a ribosomal protein." Bland M.M., Levings C.S. III, Matzinger D.F. Mol. Gen. Genet. 204:8-16(1986) [PubMed: 2875379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Petit Havana SR1. Tissue: Leaf.
[2]	"RNA editing of the mitochondrial atp9 transcript from tobacco." Hernould M., Mouras A., Litvak S., Araya A. Nucleic Acids Res. 20:1809-1809(1992) [PubMed: 1533714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA EDITING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04019 Genomic DNA. Translation: CAA27644.1. Sequence problems. X64423 mRNA. Translation: CAA45770.1.
RefSeq	YP_173404.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3205336.
Enzyme and pathway databases
BRENDA	3.6.3.14. 298.
Family and domain databases
InterPro	IPR000454. ATPase_F0-cplx_csu. IPR020537. ATPase_F0-cplx_csu_DDCD_BS. IPR002379. ATPase_F0/V0-cplx_csu. [Graphical view]
Gene3D	G3DSA:1.20.20.10. ATPase_F0/V0_c. 1 hit.
Pfam	PF00137. ATP-synt_C. 1 hit. [Graphical view]
PRINTS	PR00124. ATPASEC.
PROSITE	PS00605. ATPASE_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATP9_TOBAC

Accession

Primary (citable) accession number: P60116
Secondary accession number(s): P05497, P05498

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)