P60088 (ARGI_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginase
EC=3.5.3.1

Gene names

Name:	arg
Ordered Locus Names:	SA1968

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Arginase		PRO_0000173721
Regions
	Region	128 – 132	5	Substrate binding By similarity
	Region	139 – 141	3	Substrate binding By similarity
Sites
	Metal binding	103	1	Manganese 1 By similarity
	Metal binding	126	1	Manganese 1 By similarity
	Metal binding	126	1	Manganese 2 By similarity
	Metal binding	128	1	Manganese 2 By similarity
	Metal binding	130	1	Manganese 1 By similarity
	Metal binding	229	1	Manganese 1 By similarity
	Metal binding	229	1	Manganese 2 By similarity
	Metal binding	231	1	Manganese 2 By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60088 [UniParc]. Last modified November 28, 2003. Version 1. Checksum: DD98335783BAC146	FASTA	302	33,265
	10 20 30 40 50 60 MTKTKAIDII GAPSTFGQRK LGVDLGPTAI RYAGLISRLK QLDLDVYDKG DIKVPAVNIE 70 80 90 100 110 120 KFHSEQKGLR NYDEIIDVNQ KLNKEVSASI ENNRFPLVLG GDHSIAVGSV SAISKHYNNL 130 140 150 160 170 180 GVIWYDAHGD LNIPEESPSG NIHGMPLRIL TGEGPKELLE LNSNVIKPEN IVLIGMRDLD 190 200 210 220 230 240 KGERQFIKDH NIKTFTMSDI DKLGIKEVIE NTIEYLKSRN VDGVHLSLDV DALDPLETPG 250 260 270 280 290 300 TGTRVLGGLS YRESHFALEL LHQSHLISSM DLVEVNPLID SNNHTAEQAV SLVGTFFGET LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.
[2]	"Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth." Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J. J. Microbiol. Methods 60:247-257(2005) [PubMed: 15590099] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: N315.
[3]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB43257.1.
PIR	H90011.
RefSeq	NP_375278.1. NC_002745.2.
3D structure databases
ProteinModelPortal	P60088.
SMR	P60088. Positions 5-302.
ModBase	Search...
Protein-protein interaction databases
STRING	P60088.
2D gel databases
SWISS-2DPAGE	P60088.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000001264; EBSTAP00000001264; EBSTAG00000001264.
GeneID	1124874.
GenomeReviews	Gene locus SA1968 in contig BA000018_GR.
KEGG	sau:SA1968.
PATRIC	19576402. VBIStaAur116463_2128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0010.
GeneTree	EBGT00050000025100.
HOGENOM	HBG391953.
OMA	ADIPNDS.
ProtClustDB	CLSK885803.
Enzyme and pathway databases
BioCyc	SAUR158879:SA1968-MONOMER.
Family and domain databases
InterPro	IPR014033. Arginase_subgr. IPR006035. Ureohydrolase. IPR023696. Ureohydrolase_domain. IPR020855. Ureohydrolase_Mn_BS. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
KO	K01476.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PIRSF	PIRSF036979. Arginase. 1 hit.
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. RocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_STAAN

Accession

Primary (citable) accession number: P60088
Secondary accession number(s): Q9R2U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program