Reviewed, UniProtKB/Swiss-Prot P60086 (ARGI_STAAC)

Names and origin

Protein names

Recommended name:
    Arginase
    EC=3.5.3.1

Gene names

Name:	arg
Synonyms:	rocF
Ordered Locus Names:	SACOL2154

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-arginine + H2O = L-ornithine + urea.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Sequence similarities	Belongs to the arginase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arginase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Arginase		PRO_0000173719
Regions
	Region	128 – 132	5	Substrate binding By similarity
	Region	139 – 141	3	Substrate binding By similarity
Sites
	Metal binding	103	1	Manganese 1 By similarity
	Metal binding	126	1	Manganese 1 By similarity
	Metal binding	126	1	Manganese 2 By similarity
	Metal binding	128	1	Manganese 2 By similarity
	Metal binding	130	1	Manganese 1 By similarity
	Metal binding	229	1	Manganese 1 By similarity
	Metal binding	229	1	Manganese 2 By similarity
	Metal binding	231	1	Manganese 2 By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60086-1 [UniParc]. Last modified November 28, 2003. Version 1. Checksum: DD98335783BAC146	FASTA	302	33,265
	10 20 30 40 50 60 MTKTKAIDII GAPSTFGQRK LGVDLGPTAI RYAGLISRLK QLDLDVYDKG DIKVPAVNIE 70 80 90 100 110 120 KFHSEQKGLR NYDEIIDVNQ KLNKEVSASI ENNRFPLVLG GDHSIAVGSV SAISKHYNNL 130 140 150 160 170 180 GVIWYDAHGD LNIPEESPSG NIHGMPLRIL TGEGPKELLE LNSNVIKPEN IVLIGMRDLD 190 200 210 220 230 240 KGERQFIKDH NIKTFTMSDI DKLGIKEVIE NTIEYLKSRN VDGVHLSLDV DALDPLETPG 250 260 270 280 290 300 TGTRVLGGLS YRESHFALEL LHQSHLISSM DLVEVNPLID SNNHTAEQAV SLVGTFFGET LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mrp -- a new auxiliary gene essential for optimal expression of methicillin resistance in Staphylococcus aureus." Wu S.-W., de Lencastre H. Microb. Drug Resist. 5:9-18(1999) [PubMed: 10332717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y09927 Genomic DNA. Translation: CAB55326.1. Y09594 Genomic DNA. Translation: CAA70781.1. CP000046 Genomic DNA. Translation: AAW38462.1.
RefSeq	YP_186967.1.
3D structure databases
HSSP	HSSP built from PDB template 2CEV based on UniProtKB P53608.
ModBase	Search...
Protein-protein interaction databases
STRING	P60086.
Genome annotation databases
GeneID	3237431.
GenomeReviews	Gene locus SACOL2154 in contig CP000046_GR.
KEGG	sac:SACOL2154.
TIGR	SACOL2154.
Phylogenomic databases
HOGENOM	P60086.
OMA	QIVKNPR.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL2154-MON.
Family and domain databases
InterPro	IPR005924. Arginase. IPR014033. Arginase_sub. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358:SF2. Arginase_sub. 1 hit. PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
PRINTS	PR00116. ARGINASE.
TIGRFAMs	TIGR01229. rocF_arginase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGI_STAAC

Accession

Primary (citable) accession number: P60086
Secondary accession number(s): Q9R2U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)