Reviewed, UniProtKB/Swiss-Prot P60068 (CLRA_IDEDE)

Names and origin

Protein names

Recommended name:
    Chlorate reductase subunit alpha
    EC=1.97.1.1
Alternative name(s):
    Chlorate reductase molybdenum subunit

Gene names

Name:

clrA

Organism

Ideonella dechloratans

Taxonomic identifier

36863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Ideonella

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Terminal reductase that allows anaerobic growth on chlorate as the sole respiratory oxidant.
Catalytic activity	AH2 + chlorate = A + H2O + chlorite.
Cofactor	Binds 1 4Fe-4S cluster Potential. Molybdenum (molybdopterin).
Subunit structure	Heterotrimer of alpha, beta and gamma subunits.
Subcellular location	Periplasm.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Biotechnological use	Has potential use in bioremediation of waste sites contaminated with chlorate, such as pulp and paper industry wastewater.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW chlorate reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Tat-type signal Potential
	Chain	33 – 914	882	Chlorate reductase subunit alpha		PRO_0000019173
Sites
	Metal binding	69	1	Iron-sulfur (4Fe-4S); via pros nitrogen By similarity
	Metal binding	73	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	77	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	111	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	205	1	Molybdenum By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60068-1 [UniParc]. Last modified November 28, 2003. Version 1. Checksum: 372B1FDE26E06499	FASTA	914	102,823
	10 20 30 40 50 60 MNSPDEHNGR RRFLQFSAAA LASAAASPSL WAFSKIQPIE DPLKDYPYRD WEDLYRKEWT 70 80 90 100 110 120 WDSVGVMTHS NGCVAGCAWN VFVKNGIPMR EEQISKYPQL PGIPDMNPRG CQKGAVYCSW 130 140 150 160 170 180 SKQPDHIKWP LKRVGERGER KWKRISWDEA LTEIADKIID TTVKRGPGNI YIPKRPFAVI 190 200 210 220 230 240 TNTAYTRMTK LLGAISPDAT SMTGDLYTGI QTVRVPASTV STFDDWFTSD LILMWHKNPI 250 260 270 280 290 300 VTRIPDAHFL MEARYNGARL VNISADYNPS SIHSDLFVPV TSGTDSHLAA ALVNVLIAGK 310 320 330 340 350 360 HYKADYLKEQ TALPFLVRTD NGKFLREKDF KADGSDQVFY VWDTKAGKAV LAPGSMGSKD 370 380 390 400 410 420 KTLKLGTIDP ALEGNFETHG IKVTTVFERL KAEITPYTPE ATQATTGVHP SVVRQLAGWI 430 440 450 460 470 480 AECKALRILD GYNNQKHFDG FQCGRLKILI LTLIGHHGTT GSIDTTFEGW RLEGNSELGT 490 500 510 520 530 540 VKGKPGRSVS AVLAQWVWGE QYQRSKDYFN DAQLREELGF GVDEMESMRK ESEANGWMPN 550 560 570 580 590 600 WQSIKEPVVS ITGGINMFAT SNGYQHLRDN FLKRCELNVV VDFRLNSGAM YADIVLPAAE 610 620 630 640 650 660 NTEKLDIRET SVTRFIHAFG QPVKPMYERK TDWQIMVALA AKIQERAKAR GIARVDDPEI 670 680 690 700 710 720 KSGIDFDKIY DEFTMNGKVV TDEQAVRFVM DNSKALGPGT YEEVMKNGFV AVGPSAGKTG 730 740 750 760 770 780 PVPKDKPYRP FTVNVTDKKP YGTLTGRLQF YVDHDWFQRL GATVPKPQYR GGVLGPKKYP 790 800 810 820 830 840 FVRNSPHARW GVHSFARTEQ WMLRHQRGEP DVRMSPKAMA AKGIKDGDMV RIFNDSGEFF 850 860 870 880 890 900 AVVKAMPALP DNMLFTEHGW EQYQYKNMTH YNMVSSELIN PLELVGGYGH IKYTSGGFNP 910 NRIFYETTVD VEKA

References

[1]	"A gene cluster for chlorate metabolism in Ideonella dechloratans." Danielsson Thorell H., Stenklo K., Karlsson J., Nilsson T. Appl. Environ. Microbiol. 69:5585-5592(2003) [PubMed: 12957948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ566363 Genomic DNA. Translation: CAD97447.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	5.A.3.8.2. prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
Enzyme and pathway databases
BRENDA	1.97.1.1. 189170.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR017840. DMSO_Rdtase_II_Mopterin_su. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 2 hits. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR03479. DMSO_red_II_alp. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLRA_IDEDE

Accession

Primary (citable) accession number: P60068

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)