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P60061 (ADIC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arginine/agmatine antiporter
Gene names
Name:adiC
Synonyms:aniC, yjdD, yjdE
Ordered Locus Names:b4115, JW4076
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major component of the acid-resistance (AR) system allowing enteric pathogens to survive the acidic environment in the stomach By similarity. Exchanges extracellular arginine for its intracellular decarboxylation product agmatine (Agm) thereby expelling intracellular protons. Ref.4 Ref.5

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Induction

By acidic conditions. Ref.4

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Arginine/agmatine antiporter
PRO_0000054230

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3021Helical; Potential
Topological domain31 – 388Periplasmic Potential
Transmembrane39 – 5921Helical; Potential
Topological domain60 – 9839Cytoplasmic Potential
Transmembrane99 – 11921Helical; Potential
Topological domain120 – 1223Periplasmic Potential
Transmembrane123 – 14321Helical; Potential
Topological domain144 – 1529Cytoplasmic Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 19623Periplasmic Potential
Transmembrane197 – 21721Helical; Potential
Topological domain218 – 2258Cytoplasmic Potential
Transmembrane226 – 24621Helical; Potential
Topological domain247 – 27529Periplasmic Potential
Transmembrane276 – 29621Helical; Potential
Topological domain297 – 32125Cytoplasmic Potential
Transmembrane322 – 34221Helical; Potential
Topological domain343 – 35513Periplasmic Potential
Transmembrane356 – 37621Helical; Potential
Topological domain377 – 3859Cytoplasmic Potential
Transmembrane386 – 40621Helical; Potential
Topological domain407 – 4082Periplasmic Potential
Transmembrane409 – 42921Helical; Potential
Topological domain430 – 44516Cytoplasmic Potential

Sites

Binding site221Substrate Probable
Binding site261Substrate Probable
Binding site931Substrate By similarity
Binding site2081Substrate Probable
Binding site3651Substrate By similarity
Site2081Proposed to be the sensor amino acid remaining protonated at periplasmic pH but undergoing deprotonation at cytoplasmic pH By similarity

Secondary structure

1 ............................................................ 445
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60061 [UniParc].

Last modified November 28, 2003. Version 1.
Checksum: 359F70C489A20663

FASTA44546,843
        10         20         30         40         50         60 
MSSDADAHKV GLIPVTLMVS GNIMGSGVFL LPANLASTGG IAIYGWLVTI IGALGLSMVY 

        70         80         90        100        110        120 
AKMSFLDPSP GGSYAYARRC FGPFLGYQTN VLYWLACWIG NIAMVVIGVG YLSYFFPILK 

       130        140        150        160        170        180 
DPLVLTITCV VVLWIFVLLN IVGPKMITRV QAVATVLALI PIVGIAVFGW FWFRGETYMA 

       190        200        210        220        230        240 
AWNVSGLGTF GAIQSTLNVT LWSFIGVESA SVAAGVVKNP KRNVPIATIG GVLIAAVCYV 

       250        260        270        280        290        300 
LSTTAIMGMI PNAALRVSAS PFGDAARMAL GDTAGAIVSF CAAAGCLGSL GGWTLLAGQT 

       310        320        330        340        350        360 
AKAAADDGLF PPIFARVNKA GTPVAGLIIV GILMTIFQLS SISPNATKEF GLVSSVSVIF 

       370        380        390        400        410        420 
TLVPYLYTCA ALLLLGHGHF GKARPAYLAV TTIAFLYCIW AVVGSGAKEV MWSFVTLMVI 

       430        440 
TAMYALNYNR LHKNPYPLDA PISKD

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"YjdE (AdiC) is the arginine:agmatine antiporter essential for arginine-dependent acid resistance in Escherichia coli."
Gong S., Richard H., Foster J.W.
J. Bacteriol. 185:4402-4409(2003) [PubMed: 12867448] [Abstract]
Cited for: FUNCTION, INDUCTION.
Strain: K12.
[5]"Arginine-agmatine antiporter in extreme acid resistance in Escherichia coli."
Iyer R., Williams C., Miller C.
J. Bacteriol. 185:6556-6561(2003) [PubMed: 14594828] [Abstract]
Cited for: FUNCTION.
Strain: K12 / MG1655 / ATCC 47076.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U14003 Genomic DNA. Translation: AAA97015.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97014.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77076.1.
AP009048 Genomic DNA. Translation: BAE78117.1.
PIRB65221.
S56343.
RefSeqNP_418539.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3OB6X-ray3.00A/B1-445[»]
ProteinModelPortalP60061.
SMRP60061. Positions 5-440.
ModBaseSearch...

Protein family/group databases

TCDB2.A.3.2.5. amino acid-polyamine-organocation (APC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000003768; EBESCP00000003768; EBESCG00000003079.
EBESCT00000018190; EBESCP00000017481; EBESCG00000017245.
GeneID948628.
GenomeReviewsGene locus JW4076 in contig AP009048_GR.
Gene locus b4115 in contig U00096_GR.
KEGGecj:JW4076.
eco:b4115.
PATRIC32123795. VBIEscCol129921_4246.

Organism-specific databases

EchoBASEEB2355.
EcoGeneEG12462. adiC.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeEBGT00050000009006.
HOGENOMHBG586921.
OMAMSPSASE.
PhylomeDBP60061.
ProtClustDBPRK10644.

Enzyme and pathway databases

BioCycEcoCyc:YJDE-MONOMER.

Gene expression databases

GenevestigatorP60061.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR002293. AA/rel_permease1.
[Graphical view]
KOK03759.
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
ProtoNetSearch...

Entry information

Entry nameADIC_ECOLI
AccessionPrimary (citable) accession number: P60061
Secondary accession number(s): P39268, P39269, Q2M6I9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents