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Protein

Arginine/agmatine antiporter

Gene

adiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major component of the acid-resistance (AR) system allowing enteric pathogens to survive the acidic environment in the stomach (By similarity). Exchanges extracellular arginine for its intracellular decarboxylation product agmatine (Agm) thereby expelling intracellular protons.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22SubstrateCurated1
Binding sitei26SubstrateCurated1
Binding sitei93SubstrateBy similarity1
Binding sitei208SubstrateCurated1
Sitei208Proposed to be the sensor amino acid remaining protonated at periplasmic pH but undergoing deprotonation at cytoplasmic pHBy similarity1
Binding sitei365SubstrateBy similarity1

GO - Molecular functioni

  • antiporter activity Source: EcoCyc
  • arginine transmembrane transporter activity Source: EcoCyc
  • L-amino acid transmembrane transporter activity Source: GO_Central

GO - Biological processi

  • amino acid transmembrane transport Source: EcoCyc
  • intracellular pH elevation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJDE-MONOMER.
ECOL316407:JW4076-MONOMER.
MetaCyc:YJDE-MONOMER.

Protein family/group databases

TCDBi2.A.3.2.5. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine/agmatine antiporter
Gene namesi
Name:adiC
Synonyms:aniC, yjdD, yjdE
Ordered Locus Names:b4115, JW4076
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12462. adiC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Topological domaini31 – 38PeriplasmicSequence analysis8
Transmembranei39 – 59HelicalSequence analysisAdd BLAST21
Topological domaini60 – 98CytoplasmicSequence analysisAdd BLAST39
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 122PeriplasmicSequence analysis3
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 152CytoplasmicSequence analysis9
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 196PeriplasmicSequence analysisAdd BLAST23
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 225CytoplasmicSequence analysis8
Transmembranei226 – 246HelicalSequence analysisAdd BLAST21
Topological domaini247 – 275PeriplasmicSequence analysisAdd BLAST29
Transmembranei276 – 296HelicalSequence analysisAdd BLAST21
Topological domaini297 – 321CytoplasmicSequence analysisAdd BLAST25
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 355PeriplasmicSequence analysisAdd BLAST13
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 385CytoplasmicSequence analysis9
Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
Topological domaini407 – 408PeriplasmicSequence analysis2
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21
Topological domaini430 – 445CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542301 – 445Arginine/agmatine antiporterAdd BLAST445

Proteomic databases

PaxDbiP60061.
PRIDEiP60061.

Expressioni

Inductioni

By acidic conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263081. 6 interactors.
DIPiDIP-59619N.
STRINGi511145.b4115.

Structurei

Secondary structure

1445
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Helixi12 – 24Combined sources13
Turni25 – 30Combined sources6
Helixi31 – 35Combined sources5
Turni36 – 38Combined sources3
Helixi42 – 66Combined sources25
Turni70 – 72Combined sources3
Helixi73 – 81Combined sources9
Helixi83 – 111Combined sources29
Helixi112 – 114Combined sources3
Helixi117 – 119Combined sources3
Helixi122 – 141Combined sources20
Helixi144 – 168Combined sources25
Helixi169 – 172Combined sources4
Helixi175 – 180Combined sources6
Helixi189 – 200Combined sources12
Helixi201 – 203Combined sources3
Turni205 – 208Combined sources4
Helixi209 – 212Combined sources4
Helixi213 – 216Combined sources4
Beta strandi217 – 219Combined sources3
Helixi220 – 247Combined sources28
Helixi252 – 256Combined sources5
Helixi261 – 269Combined sources9
Helixi274 – 306Combined sources33
Helixi312 – 315Combined sources4
Helixi324 – 339Combined sources16
Helixi352 – 360Combined sources9
Helixi362 – 376Combined sources15
Helixi377 – 383Combined sources7
Helixi384 – 403Combined sources20
Helixi407 – 428Combined sources22
Turni429 – 431Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OB6X-ray3.00A/B1-445[»]
5J4IX-ray2.21A/B1-445[»]
5J4NX-ray2.59A/B1-445[»]
ProteinModelPortaliP60061.
SMRiP60061.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164506.
InParanoidiP60061.
KOiK03759.
OMAiNAWISPS.
PhylomeDBiP60061.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.

Sequencei

Sequence statusi: Complete.

P60061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDADAHKV GLIPVTLMVS GNIMGSGVFL LPANLASTGG IAIYGWLVTI
60 70 80 90 100
IGALGLSMVY AKMSFLDPSP GGSYAYARRC FGPFLGYQTN VLYWLACWIG
110 120 130 140 150
NIAMVVIGVG YLSYFFPILK DPLVLTITCV VVLWIFVLLN IVGPKMITRV
160 170 180 190 200
QAVATVLALI PIVGIAVFGW FWFRGETYMA AWNVSGLGTF GAIQSTLNVT
210 220 230 240 250
LWSFIGVESA SVAAGVVKNP KRNVPIATIG GVLIAAVCYV LSTTAIMGMI
260 270 280 290 300
PNAALRVSAS PFGDAARMAL GDTAGAIVSF CAAAGCLGSL GGWTLLAGQT
310 320 330 340 350
AKAAADDGLF PPIFARVNKA GTPVAGLIIV GILMTIFQLS SISPNATKEF
360 370 380 390 400
GLVSSVSVIF TLVPYLYTCA ALLLLGHGHF GKARPAYLAV TTIAFLYCIW
410 420 430 440
AVVGSGAKEV MWSFVTLMVI TAMYALNYNR LHKNPYPLDA PISKD
Length:445
Mass (Da):46,843
Last modified:November 28, 2003 - v1
Checksum:i359F70C489A20663
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97015.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97014.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77076.1.
AP009048 Genomic DNA. Translation: BAE78117.1.
PIRiB65221.
S56343.
RefSeqiNP_418539.1. NC_000913.3.
WP_000093154.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77076; AAC77076; b4115.
BAE78117; BAE78117; BAE78117.
GeneIDi948628.
KEGGiecj:JW4076.
eco:b4115.
PATRICi32123795. VBIEscCol129921_4246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97015.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97014.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77076.1.
AP009048 Genomic DNA. Translation: BAE78117.1.
PIRiB65221.
S56343.
RefSeqiNP_418539.1. NC_000913.3.
WP_000093154.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OB6X-ray3.00A/B1-445[»]
5J4IX-ray2.21A/B1-445[»]
5J4NX-ray2.59A/B1-445[»]
ProteinModelPortaliP60061.
SMRiP60061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263081. 6 interactors.
DIPiDIP-59619N.
STRINGi511145.b4115.

Protein family/group databases

TCDBi2.A.3.2.5. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP60061.
PRIDEiP60061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77076; AAC77076; b4115.
BAE78117; BAE78117; BAE78117.
GeneIDi948628.
KEGGiecj:JW4076.
eco:b4115.
PATRICi32123795. VBIEscCol129921_4246.

Organism-specific databases

EchoBASEiEB2355.
EcoGeneiEG12462. adiC.

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164506.
InParanoidiP60061.
KOiK03759.
OMAiNAWISPS.
PhylomeDBiP60061.

Enzyme and pathway databases

BioCyciEcoCyc:YJDE-MONOMER.
ECOL316407:JW4076-MONOMER.
MetaCyc:YJDE-MONOMER.

Miscellaneous databases

PROiP60061.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADIC_ECOLI
AccessioniPrimary (citable) accession number: P60061
Secondary accession number(s): P39268, P39269, Q2M6I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.