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Protein

Arginine/agmatine antiporter

Gene

adiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major component of the acid-resistance (AR) system allowing enteric pathogens to survive the acidic environment in the stomach (By similarity). Exchanges extracellular arginine for its intracellular decarboxylation product agmatine (Agm) thereby expelling intracellular protons.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221SubstrateCurated
Binding sitei26 – 261SubstrateCurated
Binding sitei93 – 931SubstrateBy similarity
Binding sitei208 – 2081SubstrateCurated
Sitei208 – 2081Proposed to be the sensor amino acid remaining protonated at periplasmic pH but undergoing deprotonation at cytoplasmic pHBy similarity
Binding sitei365 – 3651SubstrateBy similarity

GO - Molecular functioni

  • antiporter activity Source: EcoCyc
  • arginine transmembrane transporter activity Source: EcoCyc
  • L-amino acid transmembrane transporter activity Source: GO_Central

GO - Biological processi

  • amino acid transmembrane transport Source: EcoCyc
  • intracellular pH elevation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJDE-MONOMER.
ECOL316407:JW4076-MONOMER.
MetaCyc:YJDE-MONOMER.

Protein family/group databases

TCDBi2.A.3.2.5. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine/agmatine antiporter
Gene namesi
Name:adiC
Synonyms:aniC, yjdD, yjdE
Ordered Locus Names:b4115, JW4076
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12462. adiC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Topological domaini31 – 388PeriplasmicSequence analysis
Transmembranei39 – 5921HelicalSequence analysisAdd
BLAST
Topological domaini60 – 9839CytoplasmicSequence analysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence analysisAdd
BLAST
Topological domaini120 – 1223PeriplasmicSequence analysis
Transmembranei123 – 14321HelicalSequence analysisAdd
BLAST
Topological domaini144 – 1529CytoplasmicSequence analysis
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Topological domaini174 – 19623PeriplasmicSequence analysisAdd
BLAST
Transmembranei197 – 21721HelicalSequence analysisAdd
BLAST
Topological domaini218 – 2258CytoplasmicSequence analysis
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Topological domaini247 – 27529PeriplasmicSequence analysisAdd
BLAST
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Topological domaini297 – 32125CytoplasmicSequence analysisAdd
BLAST
Transmembranei322 – 34221HelicalSequence analysisAdd
BLAST
Topological domaini343 – 35513PeriplasmicSequence analysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST
Topological domaini377 – 3859CytoplasmicSequence analysis
Transmembranei386 – 40621HelicalSequence analysisAdd
BLAST
Topological domaini407 – 4082PeriplasmicSequence analysis
Transmembranei409 – 42921HelicalSequence analysisAdd
BLAST
Topological domaini430 – 44516CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Arginine/agmatine antiporterPRO_0000054230Add
BLAST

Proteomic databases

PaxDbiP60061.

Expressioni

Inductioni

By acidic conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263081. 6 interactions.
DIPiDIP-59619N.
STRINGi511145.b4115.

Structurei

Secondary structure

1
445
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83Combined sources
Helixi12 – 2413Combined sources
Turni25 – 306Combined sources
Helixi31 – 355Combined sources
Turni36 – 383Combined sources
Helixi42 – 6625Combined sources
Turni70 – 723Combined sources
Helixi73 – 819Combined sources
Helixi83 – 11129Combined sources
Helixi112 – 1143Combined sources
Helixi117 – 1193Combined sources
Helixi122 – 14120Combined sources
Helixi144 – 16825Combined sources
Helixi169 – 1724Combined sources
Helixi175 – 1806Combined sources
Helixi189 – 20012Combined sources
Helixi201 – 2033Combined sources
Turni205 – 2084Combined sources
Helixi209 – 2124Combined sources
Helixi213 – 2164Combined sources
Beta strandi217 – 2193Combined sources
Helixi220 – 24728Combined sources
Helixi252 – 2565Combined sources
Helixi261 – 2699Combined sources
Helixi274 – 30633Combined sources
Helixi312 – 3154Combined sources
Helixi324 – 33916Combined sources
Helixi352 – 3609Combined sources
Helixi362 – 37615Combined sources
Helixi377 – 3837Combined sources
Helixi384 – 40320Combined sources
Helixi407 – 42822Combined sources
Turni429 – 4313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OB6X-ray3.00A/B1-445[»]
ProteinModelPortaliP60061.
SMRiP60061. Positions 5-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164506.
InParanoidiP60061.
KOiK03759.
OMAiNAWISPS.
PhylomeDBiP60061.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.

Sequencei

Sequence statusi: Complete.

P60061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDADAHKV GLIPVTLMVS GNIMGSGVFL LPANLASTGG IAIYGWLVTI
60 70 80 90 100
IGALGLSMVY AKMSFLDPSP GGSYAYARRC FGPFLGYQTN VLYWLACWIG
110 120 130 140 150
NIAMVVIGVG YLSYFFPILK DPLVLTITCV VVLWIFVLLN IVGPKMITRV
160 170 180 190 200
QAVATVLALI PIVGIAVFGW FWFRGETYMA AWNVSGLGTF GAIQSTLNVT
210 220 230 240 250
LWSFIGVESA SVAAGVVKNP KRNVPIATIG GVLIAAVCYV LSTTAIMGMI
260 270 280 290 300
PNAALRVSAS PFGDAARMAL GDTAGAIVSF CAAAGCLGSL GGWTLLAGQT
310 320 330 340 350
AKAAADDGLF PPIFARVNKA GTPVAGLIIV GILMTIFQLS SISPNATKEF
360 370 380 390 400
GLVSSVSVIF TLVPYLYTCA ALLLLGHGHF GKARPAYLAV TTIAFLYCIW
410 420 430 440
AVVGSGAKEV MWSFVTLMVI TAMYALNYNR LHKNPYPLDA PISKD
Length:445
Mass (Da):46,843
Last modified:November 28, 2003 - v1
Checksum:i359F70C489A20663
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97015.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97014.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77076.1.
AP009048 Genomic DNA. Translation: BAE78117.1.
PIRiB65221.
S56343.
RefSeqiNP_418539.1. NC_000913.3.
WP_000093154.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77076; AAC77076; b4115.
BAE78117; BAE78117; BAE78117.
GeneIDi948628.
KEGGiecj:JW4076.
eco:b4115.
PATRICi32123795. VBIEscCol129921_4246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97015.1. Frameshift.
U14003 Genomic DNA. Translation: AAA97014.1. Frameshift.
U00096 Genomic DNA. Translation: AAC77076.1.
AP009048 Genomic DNA. Translation: BAE78117.1.
PIRiB65221.
S56343.
RefSeqiNP_418539.1. NC_000913.3.
WP_000093154.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OB6X-ray3.00A/B1-445[»]
ProteinModelPortaliP60061.
SMRiP60061. Positions 5-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263081. 6 interactions.
DIPiDIP-59619N.
STRINGi511145.b4115.

Protein family/group databases

TCDBi2.A.3.2.5. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP60061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77076; AAC77076; b4115.
BAE78117; BAE78117; BAE78117.
GeneIDi948628.
KEGGiecj:JW4076.
eco:b4115.
PATRICi32123795. VBIEscCol129921_4246.

Organism-specific databases

EchoBASEiEB2355.
EcoGeneiEG12462. adiC.

Phylogenomic databases

eggNOGiENOG4105D5Q. Bacteria.
COG0531. LUCA.
HOGENOMiHOG000164506.
InParanoidiP60061.
KOiK03759.
OMAiNAWISPS.
PhylomeDBiP60061.

Enzyme and pathway databases

BioCyciEcoCyc:YJDE-MONOMER.
ECOL316407:JW4076-MONOMER.
MetaCyc:YJDE-MONOMER.

Miscellaneous databases

PROiP60061.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADIC_ECOLI
AccessioniPrimary (citable) accession number: P60061
Secondary accession number(s): P39268, P39269, Q2M6I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.