Reviewed, UniProtKB/Swiss-Prot P60053 (ALF1_PORGI)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase class 1
    EC=4.1.2.13
Alternative name(s):
    Fructose-biphosphate aldolase class I
      Short name=FBP aldolase

Gene names

Name:	fda
Synonyms:	fbaB
Ordered Locus Names:	PG_1755

Organism

Porphyromonas gingivalis (Bacteroides gingivalis) [Complete proteome] [HAMAP]

Taxonomic identifier

837 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 293

293

Fructose-bisphosphate aldolase class 1 HAMAP MF_00729

PRO_0000216902

Sites

Active site

176

1

Proton acceptor By similarity

Active site

211

1

Schiff-base intermediate with dihydroxyacetone-P By similarity

Secondary structure

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293

Helix Strand Turn

Details...

Helix

1 – 11

11

Beta strand

14 – 19

6

Helix

23 – 25

3

Helix

26 – 32

7

Helix

37 – 39

3

Helix

43 – 59

17

Helix

61 – 64

4

Beta strand

65 – 73

9

Helix

75 – 78

4

Beta strand

80 – 82

3

Helix

87 – 95

9

Beta strand

98 – 102

5

Helix

123 – 132

10

Beta strand

137 – 144

8

Helix

149 – 167

19

Turn

168 – 170

3

Beta strand

172 – 179

8

Helix

186 – 203

18

Beta strand

208 – 213

6

Turn

218 – 221

4

Helix

222 – 225

4

Beta strand

230 – 236

7

Helix

242 – 249

8

Beta strand

256 – 260

5

Helix

261 – 264

4

Helix

273 – 292

20

Sequences

	Sequence		Length	Mass (Da)
	P60053-1 [UniParc]. Last modified November 21, 2003. Version 1. Checksum: 8BF85BFF649DCE93	FASTA	293	32,987
	10 20 30 40 50 60 MNKEQLQQMR QAPGFVGALD QSGGSTPKAL KAYGIQPDAY QSEEEMFDLI HQMRTRMITS 70 80 90 100 110 120 PAFATGKIIG VILFERTMRG KIEGMPTADF LWEKRHIVPF LKVDKGLQDE ANGVQLMKPF 130 140 150 160 170 180 PELGKLCEEA VGYHVFGTKM RSVIKQANEQ GIRDIVEQQF QWGKEILSHG LVPILEPEVD 190 200 210 220 230 240 IHCPEKAKAE EILKRELLAQ LDKMTEPVML KITIPTVDNF YKEIIEHPMM LRVVALSGGY 250 260 270 280 290 SREQANELLS RNHGVIASFS RALVEGLSAR QTDAEFNAML EASIEDVYQA SIK

References

[1]

"Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83." Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J., Granger D., Tettelin H. , Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., Fraser C.M. J. Bacteriol. 185:5591-5601(2003) [PubMed: 12949112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-308 / W83.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE015924 Genomic DNA. Translation: AAQ66757.1.

RefSeq

NP_905858.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IQT	X-ray	2.46	A	1-293	[»]

ModBase

Search...

Genome annotation databases

GeneID

2552379.

GenomeReviews

Gene locus PG_1755 in contig AE015924_GR.

KEGG

pgi:PG1755.

NMPDR

fig|242619.1.peg.1499.

TIGR

PG_1755.

Phylogenomic databases

HOGENOM

P60053.

OMA

GTKMRSV.

Enzyme and pathway databases

BioCyc

PGIN242619:PG1755-MON.

BRENDA

4.1.2.13. 18777.

Family and domain databases

HAMAP

MF_00729.
[Tree]

InterPro

IPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PROSITE

PS00158. ALDOLASE_CLASS_I. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ALF1_PORGI

Accession

Primary (citable) accession number: P60053

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	November 21, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)