P60051 (UBP14_PANTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thiolesterase 14
Ubiquitin-specific-processing protease 14

Gene names

Name:

USP14

Organism

Pan troglodytes (Chimpanzee)

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pan

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Homodimer Potential. Associates with the 26S proteasome. Interacts with FANCC, CXCR4 and ERN1 By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP14/UBP6 subfamily. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cell membrane Cytoplasm Membrane Proteasome
Molecular function	Hydrolase Protease Thiol protease
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 493	492	Ubiquitin carboxyl-terminal hydrolase 14		PRO_0000080638
Regions
	Domain	4 – 80	77	Ubiquitin-like
Sites
	Active site	114	1	Nucleophile By similarity
	Active site	435	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	136	1	Phosphotyrosine By similarity
	Modified residue	143	1	Phosphoserine By similarity
	Modified residue	222	1	Phosphoserine By similarity
	Modified residue	291	1	N6-acetyllysine By similarity
	Modified residue	313	1	N6-acetyllysine By similarity
	Modified residue	449	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P60051 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F4679A86E9DF00FF	FASTA	493	55,941
	10 20 30 40 50 60 MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKDDDWGN 70 80 90 100 110 120 IKIKNGMTLL MMGSADALPE EPSAKTVFVE DMTEEQLASA MELPCGLTNL GNTCYMNATV 130 140 150 160 170 180 QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI PPIILLQFLH 190 200 210 220 230 240 MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSVKETDSSS ASAATPSKKK 250 260 270 280 290 300 SLIDQFFGVE FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK 310 320 330 340 350 360 QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP LMLDMYELCT 370 380 390 400 410 420 PELQEKMVSF RSKFKDLEDK KVNQQPNTSD KKSSPQKEVK YEPFSFADDI GSNNCGYYDL 430 440 450 460 470 480 QAVLTHQGRS SSSGHYVSWV KRKQDEWIKF DDDKVSIVTP EDILRLSGGG DWHIAYVLLY 490 GPRRVEIMEE ESE

References

[1]	"Inversion, duplication, and changes in gene context are associated with human chromosome 18 evolution." Dennehey B.K., Gutches D.G., McConkey E.H., Krauter K.S. Genomics 83:493-501(2004) [PubMed: 14962675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY191612 Genomic DNA. Translation: AAP23261.1.
RefSeq	NP_001038961.1. NM_001045496.1.
UniGene	Ptr.5420.
3D structure databases
ProteinModelPortal	P60051.
SMR	P60051. Positions 6-86, 98-483.
ModBase	Search...
Protein-protein interaction databases
STRING	P60051.
Protein family/group databases
MEROPS	C19.015.
Proteomic databases
PRIDE	P60051.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPTRT00000018031; ENSPTRP00000016705; ENSPTRG00000009815.
GeneID	455325.
KEGG	ptr:455325.
Organism-specific databases
CTD	9097.
Phylogenomic databases
eggNOG	maNOG07913.
GeneTree	ENSGT00390000009615.
HOVERGEN	HBG054185.
InParanoid	P60051.
OMA	KRKEDEW.
OrthoDB	EOG4JT05C.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019955. Ubiquitin_supergroup. [Graphical view]
KO	K11843.
Pfam	PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP14_PANTR

Accession

Primary (citable) accession number: P60051

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program