Reviewed, UniProtKB/Swiss-Prot P60038 (HSS2_SENVE)

Names and origin

Protein names	Recommended name:     Homospermidine synthase 2       Short name=HSS2     EC=2.5.1.45
Organism	Senecio vernalis (Spring groundsel)
Taxonomic identifier	93496 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Senecioneae › Senecio

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the transfer of an aminobutyl unit from spermidine onto putrescine. The resulting polyamine homospermidine is a precursor in the biosynthesis of pyrrolizidine alkaloids.
Catalytic activity	Spermidine + putrescine = sym-homospermidine + propane-1,3-diamine.
Cofactor	NAD.
Pathway	Alkaloid biosynthesis; pyrrolizidine alkaloid biosynthesis.
Subunit structure	Homotetramer.
Tissue specificity	Expressed in roots.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the deoxyhypusine synthase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro
Molecular function	homospermidine synthase (spermidine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Homospermidine synthase 2		PRO_0000134518

Sequences

	Sequence		Length	Mass (Da)
	P60038-1 [UniParc]. Last modified November 21, 2003. Version 1. Checksum: F4A65775E749DE5C	FASTA	370	40,733
	10 20 30 40 50 60 MAESNKEAID SARSNVFKES ESLEGTCAKI GGYDFNNGID HSKLLKSMVS TGFQASNLGD 70 80 90 100 110 120 AMIITNQMLE WRLSHDEVPE HCSEEEKKNR ESVKCKIFLG FTSNLISSGV RETICYLAQH 130 140 150 160 170 180 RMVDVLVTTT GGIEEDFIKC LASTYKGKFS LPGADLRSKG LNRIGNLIVP NDNYIKFEDW 190 200 210 220 230 240 IIPIFDQMLI EQKTQNVLWT PSRMIARLGK EINNETSYLY WAYKNNIPVF CPSITDGSIG 250 260 270 280 290 300 DMLYFHSVSN PGPGLVVDIV QDVIAMDNEA VHASPQKTGI IILGGGLPKH HICNANMMRN 310 320 330 340 350 360 GADFAVFINT AQEYDGSDSG ARPDEAVSWG KISSTGKAVK VHCDATIAFP LLVAETFAVK 370 KEKASKVNGF

References

[1]	"Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase." Ober D., Hartmann T. Proc. Natl. Acad. Sci. U.S.A. 96:14777-14782(1999) [PubMed: 10611289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 7-17; 151-159; 211-224 AND 278-289. Tissue: Root.
[2]	"Cloning and expression of homospermidine synthase from Senecio vulgaris: a revision." Ober D., Harms R., Hartmann T. Phytochemistry 55:305-309(2000) [PubMed: 11117877] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1DHS based on UniProtKB P49366.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.45. 267997.
Family and domain databases
InterPro	IPR002773. Deoxyhypus_synth. [Graphical view]
Gene3D	G3DSA:3.40.910.10. Deoxyhypus_synth. 1 hit.
PANTHER	PTHR11703. Deoxyhypus_synth. 1 hit.
Pfam	PF01916. DS. 1 hit. [Graphical view]
ProDom	PD007730. Deoxyhypus_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00321. dhys. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HSS2_SENVE

Accession

Primary (citable) accession number: P60038

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	November 21, 2003
Last modified:	January 20, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)