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P60033

- CD81_HUMAN

UniProt

P60033 - CD81_HUMAN

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Protein
CD81 antigen
Gene
CD81, TAPA1, TSPAN28
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.

GO - Molecular functioni

  1. MHC class II protein complex binding Source: UniProtKB
  2. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. activation of MAPK activity Source: UniProtKB
  2. cell proliferation Source: ProtInc
  3. phosphatidylinositol biosynthetic process Source: UniProtKB
  4. phosphatidylinositol metabolic process Source: UniProtKB
  5. positive regulation of 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
  6. positive regulation of cell proliferation Source: UniProtKB
  7. positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  8. protein localization Source: UniProtKB
  9. receptor-mediated virion attachment to host cell Source: UniProtKB
  10. regulation of immune response Source: Reactome
  11. viral entry into host cell Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Enzyme and pathway databases

ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
SignaLinkiP60033.

Protein family/group databases

TCDBi8.A.40.1.1. the tetraspanin (tetraspanin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
CD81 antigen
Alternative name(s):
26 kDa cell surface protein TAPA-1
Target of the antiproliferative antibody 1
Tetraspanin-28
Short name:
Tspan-28
CD_antigen: CD81
Gene namesi
Name:CD81
Synonyms:TAPA1, TSPAN28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:1701. CD81.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei13 – 3321Helical; Reviewed prediction
Add
BLAST
Topological domaini34 – 6330Extracellular Reviewed prediction
Add
BLAST
Transmembranei64 – 8421Helical; Reviewed prediction
Add
BLAST
Topological domaini85 – 895Cytoplasmic Reviewed prediction
Transmembranei90 – 11223Helical; Reviewed prediction
Add
BLAST
Topological domaini113 – 20189Extracellular Reviewed prediction
Add
BLAST
Transmembranei202 – 22423Helical; Reviewed prediction
Add
BLAST
Topological domaini225 – 23612Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
  2. immunological synapse Source: UniProtKB
  3. integral component of plasma membrane Source: UniProtKB
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Immunodeficiency, common variable, 6 (CVID6) [MIM:613496]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
Note: The disease is caused by mutations affecting the gene represented in this entry.1 Publication

Organism-specific databases

MIMi613496. phenotype.
Orphaneti1572. Common variable immunodeficiency.
PharmGKBiPA26240.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236CD81 antigen
PRO_0000219221Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi156 ↔ 190
Disulfide bondi157 ↔ 175

Post-translational modificationi

Not glycosylated Inferred.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP60033.
PaxDbiP60033.
PRIDEiP60033.

PTM databases

PhosphoSiteiP60033.

Expressioni

Tissue specificityi

Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

Gene expression databases

ArrayExpressiP60033.
BgeeiP60033.
CleanExiHS_CD81.
GenevestigatoriP60033.

Organism-specific databases

HPAiCAB002507.
HPA007234.

Interactioni

Subunit structurei

Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP. Interacts with IFITM1, IFITM2 and IFITM3. Part of a GPCR-tetraspanin complex consisting at least of GPR56, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of GPR56 with GNA11.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P279589EBI-712921,EBI-6904269From a different organism.

Protein-protein interaction databases

BioGridi107413. 199 interactions.
IntActiP60033. 10 interactions.
MINTiMINT-5001104.
STRINGi9606.ENSP00000263645.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi116 – 13621
Helixi141 – 15414
Beta strandi158 – 1603
Helixi163 – 1653
Helixi166 – 1716
Helixi172 – 1743
Helixi181 – 1855
Helixi190 – 19910

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8QX-ray1.60A/B113-201[»]
1IV5X-ray2.60A/B113-201[»]
2AVZmodel-A1-236[»]
4BKHX-ray1.40A/B112-201[»]
ProteinModelPortaliP60033.
SMRiP60033. Positions 113-201.

Miscellaneous databases

EvolutionaryTraceiP60033.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG305712.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
KOiK06508.
PhylomeDBiP60033.
TreeFamiTF352895.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60033-1 [UniParc]FASTAAdd to Basket

« Hide

MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTNLLYLELG    50
DKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI 100
LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVVDDDA NNAKAVVKTF 150
HETLDCCGSS TLTALTTSVL KNNLCPSGSN IISNLFKEDC HQKIDDLFSG 200
KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY 236
Length:236
Mass (Da):25,809
Last modified:November 21, 2003 - v1
Checksum:iEB9BD7671AC91B4A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33680 mRNA. Translation: AAA36663.1.
BT019507 mRNA. Translation: AAV38314.1.
BT019508 mRNA. Translation: AAV38315.1.
EF064749 Genomic DNA. Translation: ABK41932.1.
AC129929 Genomic DNA. No translation available.
AC124057 Genomic DNA. No translation available.
BC002978 mRNA. Translation: AAH02978.1.
BC093047 mRNA. Translation: AAH93047.1.
CCDSiCCDS7734.1.
PIRiA35649.
RefSeqiNP_004347.1. NM_004356.3.
UniGeneiHs.54457.

Genome annotation databases

EnsembliENST00000263645; ENSP00000263645; ENSG00000110651.
GeneIDi975.
KEGGihsa:975.
UCSCiuc001lwf.1. human.

Polymorphism databases

DMDMi38503376.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33680 mRNA. Translation: AAA36663.1 .
BT019507 mRNA. Translation: AAV38314.1 .
BT019508 mRNA. Translation: AAV38315.1 .
EF064749 Genomic DNA. Translation: ABK41932.1 .
AC129929 Genomic DNA. No translation available.
AC124057 Genomic DNA. No translation available.
BC002978 mRNA. Translation: AAH02978.1 .
BC093047 mRNA. Translation: AAH93047.1 .
CCDSi CCDS7734.1.
PIRi A35649.
RefSeqi NP_004347.1. NM_004356.3.
UniGenei Hs.54457.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G8Q X-ray 1.60 A/B 113-201 [» ]
1IV5 X-ray 2.60 A/B 113-201 [» ]
2AVZ model - A 1-236 [» ]
4BKH X-ray 1.40 A/B 112-201 [» ]
ProteinModelPortali P60033.
SMRi P60033. Positions 113-201.
ModBasei Search...

Protein-protein interaction databases

BioGridi 107413. 199 interactions.
IntActi P60033. 10 interactions.
MINTi MINT-5001104.
STRINGi 9606.ENSP00000263645.

Chemistry

ChEMBLi CHEMBL1075180.

Protein family/group databases

TCDBi 8.A.40.1.1. the tetraspanin (tetraspanin) family.

PTM databases

PhosphoSitei P60033.

Polymorphism databases

DMDMi 38503376.

Proteomic databases

MaxQBi P60033.
PaxDbi P60033.
PRIDEi P60033.

Protocols and materials databases

DNASUi 975.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263645 ; ENSP00000263645 ; ENSG00000110651 .
GeneIDi 975.
KEGGi hsa:975.
UCSCi uc001lwf.1. human.

Organism-specific databases

CTDi 975.
GeneCardsi GC11P002397.
HGNCi HGNC:1701. CD81.
HPAi CAB002507.
HPA007234.
MIMi 186845. gene.
613496. phenotype.
neXtProti NX_P60033.
Orphaneti 1572. Common variable immunodeficiency.
PharmGKBi PA26240.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG305712.
HOGENOMi HOG000230651.
HOVERGENi HBG002324.
KOi K06508.
PhylomeDBi P60033.
TreeFami TF352895.

Enzyme and pathway databases

Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
SignaLinki P60033.

Miscellaneous databases

ChiTaRSi CD81. human.
EvolutionaryTracei P60033.
GeneWikii CD81.
GenomeRNAii 975.
NextBioi 4090.
PROi P60033.
SOURCEi Search...

Gene expression databases

ArrayExpressi P60033.
Bgeei P60033.
CleanExi HS_CD81.
Genevestigatori P60033.

Family and domain databases

Gene3Di 1.10.1450.10. 1 hit.
InterProi IPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view ]
Pfami PF00335. Tetraspannin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002419. Tetraspanin. 1 hit.
PRINTSi PR00259. TMFOUR.
SUPFAMi SSF48652. SSF48652. 1 hit.
PROSITEi PS00421. TM4_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins."
    Oren R., Takahashi S., Doss C., Levy R., Levy S.
    Mol. Cell. Biol. 10:4007-4015(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen."
    Takahashi S., Doss C., Levy S., Levy R.
    J. Immunol. 145:2207-2213(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IFITM1.
  7. "Structure and membrane topology of TAPA-1."
    Levy S., Nguyen V.Q., Andria M.L., Takahashi S.
    J. Biol. Chem. 266:14597-14602(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  8. "EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily."
    Stipp C.S., Kolesnikova T.V., Hemler M.E.
    J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IGSF8.
  9. "CD81-dependent binding of hepatitis C virus E1E2 heterodimers."
    Cocquerel L., Kuo C.-C., Dubuisson J., Levy S.
    J. Virol. 77:10677-10683(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HCV E1/E2 ENVELOPE HETERODIMER.
  10. "Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor."
    Bartosch B., Vitelli A., Granier C., Goujon C., Dubuisson J., Pascale S., Scarselli E., Cortese R., Nicosia A., Cosset F.-L.
    J. Biol. Chem. 278:41624-41630(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HCV E1/E2 ENVELOPE HETERODIMER.
  11. "Dynamic regulation of a GPCR-tetraspanin-G protein complex on intact cells: central role of CD81 in facilitating GPR56-Galpha q/11 association."
    Little K.D., Hemler M.E., Stipp C.S.
    Mol. Biol. Cell 15:2375-2387(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRP56 AND GNA11.
  12. "CD81 gene defect in humans disrupts CD19 complex formation and leads to antibody deficiency."
    van Zelm M.C., Smet J., Adams B., Mascart F., Schandene L., Janssen F., Ferster A., Kuo C.-C., Levy S., van Dongen J.J.M., van der Burg M.
    J. Clin. Invest. 120:1265-1274(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN CVID6.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling."
    Draber P., Vonkova I., Stepanek O., Hrdinka M., Kucova M., Skopcova T., Otahal P., Angelisova P., Horejsi V., Yeung M., Weiss A., Brdicka T.
    Mol. Cell. Biol. 31:4550-4562(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CD53 AND SCIMP.
  15. "CD81 extracellular domain 3D structure: insight into the tetraspanin superfamily structural motifs."
    Kitadokoro K., Bordo D., Galli G., Petracca R., Falugi F., Abrignani S., Grandi G., Bolognesi M.
    EMBO J. 20:12-18(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 113-202.
  16. "Subunit association and conformational flexibility in the head subdomain of human CD81 large extracellular loop."
    Kitadokoro K., Ponassi M., Galli G., Petracca R., Falugi F., Grandi G., Bolognesi M.
    Biol. Chem. 383:1447-1452(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 113-201.

Entry informationi

Entry nameiCD81_HUMAN
AccessioniPrimary (citable) accession number: P60033
Secondary accession number(s): P18582, Q5U0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 21, 2003
Last modified: September 3, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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