Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P60033 (CD81_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD81 antigen
Alternative name(s):
26 kDa cell surface protein TAPA-1
Target of the antiproliferative antibody 1
Tetraspanin-28
Short name=Tspan-28
CD_antigen=CD81
Gene names
Name:CD81
Synonyms:TAPA1, TSPAN28
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.

Subunit structure

Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP. Interacts with IFITM1, IFITM2 and IFITM3. Part of a GPCR-tetraspanin complex consisting at least of GPR56, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of GPR56 with GNA11. Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.14

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

Post-translational modification

Not glycosylated Probable.

Involvement in disease

Immunodeficiency, common variable, 6 (CVID6) [MIM:613496]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.12

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
   Biological processHost-virus interaction
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Inferred from direct assay PubMed 14676841. Source: UniProtKB

cell proliferation

Traceable author statement Ref.1. Source: ProtInc

phosphatidylinositol biosynthetic process

Inferred from direct assay PubMed 14676841. Source: UniProtKB

phosphatidylinositol metabolic process

Inferred from direct assay PubMed 14676841. Source: UniProtKB

positive regulation of 1-phosphatidylinositol 4-kinase activity

Inferred from direct assay PubMed 14676841. Source: UniProtKB

positive regulation of cell proliferation

Inferred from direct assay PubMed 14676841. Source: UniProtKB

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 14676841. Source: UniProtKB

protein localization

Inferred from direct assay PubMed 14676841. Source: UniProtKB

receptor-mediated virion attachment to host cell

Traceable author statement PubMed 14676841. Source: UniProtKB

regulation of immune response

Traceable author statement. Source: Reactome

viral entry into host cell

Traceable author statement PubMed 14676841. Source: UniProtKB

   Cellular_componentextracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 19199708PubMed 23376485. Source: UniProt

immunological synapse

Inferred from direct assay Ref.14. Source: UniProtKB

integral component of plasma membrane

Inferred from direct assay PubMed 14676841. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionMHC class II protein complex binding

Inferred from direct assay PubMed 20458337. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.8PubMed 14676841Ref.14Ref.6. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

P279589EBI-712921,EBI-6904269From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 236236CD81 antigen
PRO_0000219221

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Potential
Topological domain34 – 6330Extracellular Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 895Cytoplasmic Potential
Transmembrane90 – 11223Helical; Potential
Topological domain113 – 20189Extracellular Potential
Transmembrane202 – 22423Helical; Potential
Topological domain225 – 23612Cytoplasmic Potential

Amino acid modifications

Disulfide bond156 ↔ 190
Disulfide bond157 ↔ 175

Secondary structure

............... 236
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P60033 [UniParc].

Last modified November 21, 2003. Version 1.
Checksum: EB9BD7671AC91B4A

FASTA23625,809
        10         20         30         40         50         60 
MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTNLLYLELG DKPAPNTFYV 

        70         80         90        100        110        120 
GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI LFACEVAAGI WGFVNKDQIA 

       130        140        150        160        170        180 
KDVKQFYDQA LQQAVVDDDA NNAKAVVKTF HETLDCCGSS TLTALTTSVL KNNLCPSGSN 

       190        200        210        220        230 
IISNLFKEDC HQKIDDLFSG KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY 

« Hide

References

« Hide 'large scale' references
[1]"TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins."
Oren R., Takahashi S., Doss C., Levy R., Levy S.
Mol. Cell. Biol. 10:4007-4015(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Livingston R.J., Shaffer T., McFarland I., Nguyen C.P., Stanaway I.B., Rajkumar N., Johnson E.J., da Ponte S.H., Willa H., Ahearn M.O., Bertucci C., Acklestad J., Carroll A., Swanson J., Gildersleeve H.I., Nickerson D.A.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen."
Takahashi S., Doss C., Levy S., Levy R.
J. Immunol. 145:2207-2213(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IFITM1.
[7]"Structure and membrane topology of TAPA-1."
Levy S., Nguyen V.Q., Andria M.L., Takahashi S.
J. Biol. Chem. 266:14597-14602(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
[8]"EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily."
Stipp C.S., Kolesnikova T.V., Hemler M.E.
J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IGSF8.
[9]"CD81-dependent binding of hepatitis C virus E1E2 heterodimers."
Cocquerel L., Kuo C.-C., Dubuisson J., Levy S.
J. Virol. 77:10677-10683(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HCV E1/E2 ENVELOPE HETERODIMER.
[10]"Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor."
Bartosch B., Vitelli A., Granier C., Goujon C., Dubuisson J., Pascale S., Scarselli E., Cortese R., Nicosia A., Cosset F.-L.
J. Biol. Chem. 278:41624-41630(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HCV E1/E2 ENVELOPE HETERODIMER.
[11]"Dynamic regulation of a GPCR-tetraspanin-G protein complex on intact cells: central role of CD81 in facilitating GPR56-Galpha q/11 association."
Little K.D., Hemler M.E., Stipp C.S.
Mol. Biol. Cell 15:2375-2387(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GRP56 AND GNA11.
[12]"CD81 gene defect in humans disrupts CD19 complex formation and leads to antibody deficiency."
van Zelm M.C., Smet J., Adams B., Mascart F., Schandene L., Janssen F., Ferster A., Kuo C.-C., Levy S., van Dongen J.J.M., van der Burg M.
J. Clin. Invest. 120:1265-1274(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN CVID6.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling."
Draber P., Vonkova I., Stepanek O., Hrdinka M., Kucova M., Skopcova T., Otahal P., Angelisova P., Horejsi V., Yeung M., Weiss A., Brdicka T.
Mol. Cell. Biol. 31:4550-4562(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CD53 AND SCIMP.
[15]"CD81 extracellular domain 3D structure: insight into the tetraspanin superfamily structural motifs."
Kitadokoro K., Bordo D., Galli G., Petracca R., Falugi F., Abrignani S., Grandi G., Bolognesi M.
EMBO J. 20:12-18(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 113-202.
[16]"Subunit association and conformational flexibility in the head subdomain of human CD81 large extracellular loop."
Kitadokoro K., Ponassi M., Galli G., Petracca R., Falugi F., Grandi G., Bolognesi M.
Biol. Chem. 383:1447-1452(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 113-201.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33680 mRNA. Translation: AAA36663.1.
BT019507 mRNA. Translation: AAV38314.1.
BT019508 mRNA. Translation: AAV38315.1.
EF064749 Genomic DNA. Translation: ABK41932.1.
AC129929 Genomic DNA. No translation available.
AC124057 Genomic DNA. No translation available.
BC002978 mRNA. Translation: AAH02978.1.
BC093047 mRNA. Translation: AAH93047.1.
CCDSCCDS7734.1.
PIRA35649.
RefSeqNP_004347.1. NM_004356.3.
UniGeneHs.54457.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8QX-ray1.60A/B113-201[»]
1IV5X-ray2.60A/B113-201[»]
2AVZmodel-A1-236[»]
4BKHX-ray1.40A/B112-201[»]
ProteinModelPortalP60033.
SMRP60033. Positions 113-201.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107413. 198 interactions.
IntActP60033. 10 interactions.
MINTMINT-5001104.
STRING9606.ENSP00000263645.

Chemistry

ChEMBLCHEMBL1075180.

Protein family/group databases

TCDB8.A.40.1.1. the tetraspanin (tetraspanin) family.

PTM databases

PhosphoSiteP60033.

Polymorphism databases

DMDM38503376.

Proteomic databases

MaxQBP60033.
PaxDbP60033.
PRIDEP60033.

Protocols and materials databases

DNASU975.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263645; ENSP00000263645; ENSG00000110651.
GeneID975.
KEGGhsa:975.
UCSCuc001lwf.1. human.

Organism-specific databases

CTD975.
GeneCardsGC11P002397.
HGNCHGNC:1701. CD81.
HPACAB002507.
HPA007234.
MIM186845. gene.
613496. phenotype.
neXtProtNX_P60033.
Orphanet1572. Common variable immunodeficiency.
PharmGKBPA26240.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG305712.
HOGENOMHOG000230651.
HOVERGENHBG002324.
KOK06508.
PhylomeDBP60033.
TreeFamTF352895.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
SignaLinkP60033.

Gene expression databases

ArrayExpressP60033.
BgeeP60033.
CleanExHS_CD81.
GenevestigatorP60033.

Family and domain databases

Gene3D1.10.1450.10. 1 hit.
InterProIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFPIRSF002419. Tetraspanin. 1 hit.
PRINTSPR00259. TMFOUR.
SUPFAMSSF48652. SSF48652. 1 hit.
PROSITEPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD81. human.
EvolutionaryTraceP60033.
GeneWikiCD81.
GenomeRNAi975.
NextBio4090.
PROP60033.
SOURCESearch...

Entry information

Entry nameCD81_HUMAN
AccessionPrimary (citable) accession number: P60033
Secondary accession number(s): P18582, Q5U0J6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 21, 2003
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries