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P60033

- CD81_HUMAN

UniProt

P60033 - CD81_HUMAN

Protein

CD81 antigen

Gene

CD81

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (21 Nov 2003)
      Previous versions | rss
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    Functioni

    May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.

    GO - Molecular functioni

    1. MHC class II protein complex binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. activation of MAPK activity Source: UniProtKB
    2. cell proliferation Source: ProtInc
    3. phosphatidylinositol biosynthetic process Source: UniProtKB
    4. phosphatidylinositol metabolic process Source: UniProtKB
    5. positive regulation of 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
    6. positive regulation of cell proliferation Source: UniProtKB
    7. positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
    8. protein localization Source: UniProtKB
    9. receptor-mediated virion attachment to host cell Source: UniProtKB
    10. regulation of immune response Source: Reactome
    11. viral entry into host cell Source: UniProtKB

    Keywords - Biological processi

    Host-virus interaction

    Enzyme and pathway databases

    ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    SignaLinkiP60033.

    Protein family/group databases

    TCDBi8.A.40.1.1. the tetraspanin (tetraspanin) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD81 antigen
    Alternative name(s):
    26 kDa cell surface protein TAPA-1
    Target of the antiproliferative antibody 1
    Tetraspanin-28
    Short name:
    Tspan-28
    CD_antigen: CD81
    Gene namesi
    Name:CD81
    Synonyms:TAPA1, TSPAN28
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:1701. CD81.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProtKB
    2. immunological synapse Source: UniProtKB
    3. integral component of plasma membrane Source: UniProtKB
    4. membrane Source: UniProtKB
    5. plasma membrane Source: Reactome
    6. vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Immunodeficiency, common variable, 6 (CVID6) [MIM:613496]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.

    Organism-specific databases

    MIMi613496. phenotype.
    Orphaneti1572. Common variable immunodeficiency.
    PharmGKBiPA26240.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 236236CD81 antigenPRO_0000219221Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi156 ↔ 190
    Disulfide bondi157 ↔ 175

    Post-translational modificationi

    Not glycosylated.Curated

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP60033.
    PaxDbiP60033.
    PRIDEiP60033.

    PTM databases

    PhosphoSiteiP60033.

    Expressioni

    Tissue specificityi

    Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

    Gene expression databases

    ArrayExpressiP60033.
    BgeeiP60033.
    CleanExiHS_CD81.
    GenevestigatoriP60033.

    Organism-specific databases

    HPAiCAB002507.
    HPA007234.

    Interactioni

    Subunit structurei

    Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP. Interacts with IFITM1, IFITM2 and IFITM3. Part of a GPCR-tetraspanin complex consisting at least of GPR56, CD81, eventually CD9, and GNA11 in which CD81 is enhancing the association of GPR56 with GNA11.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P279589EBI-712921,EBI-6904269From a different organism.

    Protein-protein interaction databases

    BioGridi107413. 199 interactions.
    IntActiP60033. 10 interactions.
    MINTiMINT-5001104.
    STRINGi9606.ENSP00000263645.

    Structurei

    Secondary structure

    1
    236
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi116 – 13621
    Helixi141 – 15414
    Beta strandi158 – 1603
    Helixi163 – 1653
    Helixi166 – 1716
    Helixi172 – 1743
    Helixi181 – 1855
    Helixi190 – 19910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G8QX-ray1.60A/B113-201[»]
    1IV5X-ray2.60A/B113-201[»]
    2AVZmodel-A1-236[»]
    4BKHX-ray1.40A/B112-201[»]
    ProteinModelPortaliP60033.
    SMRiP60033. Positions 113-201.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60033.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 6330ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini85 – 895CytoplasmicSequence Analysis
    Topological domaini113 – 20189ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini225 – 23612CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei90 – 11223HelicalSequence AnalysisAdd
    BLAST
    Transmembranei202 – 22423HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tetraspanin (TM4SF) family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305712.
    HOGENOMiHOG000230651.
    HOVERGENiHBG002324.
    KOiK06508.
    PhylomeDBiP60033.
    TreeFamiTF352895.

    Family and domain databases

    Gene3Di1.10.1450.10. 1 hit.
    InterProiIPR028977. CD81_LEL.
    IPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view]
    PfamiPF00335. Tetraspannin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002419. Tetraspanin. 1 hit.
    PRINTSiPR00259. TMFOUR.
    SUPFAMiSSF48652. SSF48652. 1 hit.
    PROSITEiPS00421. TM4_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60033-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVEGCTKCI KYLLFVFNFV FWLAGGVILG VALWLRHDPQ TTNLLYLELG    50
    DKPAPNTFYV GIYILIAVGA VMMFVGFLGC YGAIQESQCL LGTFFTCLVI 100
    LFACEVAAGI WGFVNKDQIA KDVKQFYDQA LQQAVVDDDA NNAKAVVKTF 150
    HETLDCCGSS TLTALTTSVL KNNLCPSGSN IISNLFKEDC HQKIDDLFSG 200
    KLYLIGIAAI VVAVIMIFEM ILSMVLCCGI RNSSVY 236
    Length:236
    Mass (Da):25,809
    Last modified:November 21, 2003 - v1
    Checksum:iEB9BD7671AC91B4A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33680 mRNA. Translation: AAA36663.1.
    BT019507 mRNA. Translation: AAV38314.1.
    BT019508 mRNA. Translation: AAV38315.1.
    EF064749 Genomic DNA. Translation: ABK41932.1.
    AC129929 Genomic DNA. No translation available.
    AC124057 Genomic DNA. No translation available.
    BC002978 mRNA. Translation: AAH02978.1.
    BC093047 mRNA. Translation: AAH93047.1.
    CCDSiCCDS7734.1.
    PIRiA35649.
    RefSeqiNP_004347.1. NM_004356.3.
    UniGeneiHs.54457.

    Genome annotation databases

    EnsembliENST00000263645; ENSP00000263645; ENSG00000110651.
    GeneIDi975.
    KEGGihsa:975.
    UCSCiuc001lwf.1. human.

    Polymorphism databases

    DMDMi38503376.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33680 mRNA. Translation: AAA36663.1 .
    BT019507 mRNA. Translation: AAV38314.1 .
    BT019508 mRNA. Translation: AAV38315.1 .
    EF064749 Genomic DNA. Translation: ABK41932.1 .
    AC129929 Genomic DNA. No translation available.
    AC124057 Genomic DNA. No translation available.
    BC002978 mRNA. Translation: AAH02978.1 .
    BC093047 mRNA. Translation: AAH93047.1 .
    CCDSi CCDS7734.1.
    PIRi A35649.
    RefSeqi NP_004347.1. NM_004356.3.
    UniGenei Hs.54457.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G8Q X-ray 1.60 A/B 113-201 [» ]
    1IV5 X-ray 2.60 A/B 113-201 [» ]
    2AVZ model - A 1-236 [» ]
    4BKH X-ray 1.40 A/B 112-201 [» ]
    ProteinModelPortali P60033.
    SMRi P60033. Positions 113-201.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107413. 199 interactions.
    IntActi P60033. 10 interactions.
    MINTi MINT-5001104.
    STRINGi 9606.ENSP00000263645.

    Chemistry

    ChEMBLi CHEMBL1075180.

    Protein family/group databases

    TCDBi 8.A.40.1.1. the tetraspanin (tetraspanin) family.

    PTM databases

    PhosphoSitei P60033.

    Polymorphism databases

    DMDMi 38503376.

    Proteomic databases

    MaxQBi P60033.
    PaxDbi P60033.
    PRIDEi P60033.

    Protocols and materials databases

    DNASUi 975.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000263645 ; ENSP00000263645 ; ENSG00000110651 .
    GeneIDi 975.
    KEGGi hsa:975.
    UCSCi uc001lwf.1. human.

    Organism-specific databases

    CTDi 975.
    GeneCardsi GC11P002397.
    HGNCi HGNC:1701. CD81.
    HPAi CAB002507.
    HPA007234.
    MIMi 186845. gene.
    613496. phenotype.
    neXtProti NX_P60033.
    Orphaneti 1572. Common variable immunodeficiency.
    PharmGKBi PA26240.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG305712.
    HOGENOMi HOG000230651.
    HOVERGENi HBG002324.
    KOi K06508.
    PhylomeDBi P60033.
    TreeFami TF352895.

    Enzyme and pathway databases

    Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    SignaLinki P60033.

    Miscellaneous databases

    ChiTaRSi CD81. human.
    EvolutionaryTracei P60033.
    GeneWikii CD81.
    GenomeRNAii 975.
    NextBioi 4090.
    PROi P60033.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P60033.
    Bgeei P60033.
    CleanExi HS_CD81.
    Genevestigatori P60033.

    Family and domain databases

    Gene3Di 1.10.1450.10. 1 hit.
    InterProi IPR028977. CD81_LEL.
    IPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view ]
    Pfami PF00335. Tetraspannin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002419. Tetraspanin. 1 hit.
    PRINTSi PR00259. TMFOUR.
    SUPFAMi SSF48652. SSF48652. 1 hit.
    PROSITEi PS00421. TM4_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins."
      Oren R., Takahashi S., Doss C., Levy R., Levy S.
      Mol. Cell. Biol. 10:4007-4015(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen."
      Takahashi S., Doss C., Levy S., Levy R.
      J. Immunol. 145:2207-2213(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IFITM1.
    7. "Structure and membrane topology of TAPA-1."
      Levy S., Nguyen V.Q., Andria M.L., Takahashi S.
      J. Biol. Chem. 266:14597-14602(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    8. "EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily."
      Stipp C.S., Kolesnikova T.V., Hemler M.E.
      J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IGSF8.
    9. "CD81-dependent binding of hepatitis C virus E1E2 heterodimers."
      Cocquerel L., Kuo C.-C., Dubuisson J., Levy S.
      J. Virol. 77:10677-10683(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HCV E1/E2 ENVELOPE HETERODIMER.
    10. "Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor."
      Bartosch B., Vitelli A., Granier C., Goujon C., Dubuisson J., Pascale S., Scarselli E., Cortese R., Nicosia A., Cosset F.-L.
      J. Biol. Chem. 278:41624-41630(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HCV E1/E2 ENVELOPE HETERODIMER.
    11. "Dynamic regulation of a GPCR-tetraspanin-G protein complex on intact cells: central role of CD81 in facilitating GPR56-Galpha q/11 association."
      Little K.D., Hemler M.E., Stipp C.S.
      Mol. Biol. Cell 15:2375-2387(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRP56 AND GNA11.
    12. "CD81 gene defect in humans disrupts CD19 complex formation and leads to antibody deficiency."
      van Zelm M.C., Smet J., Adams B., Mascart F., Schandene L., Janssen F., Ferster A., Kuo C.-C., Levy S., van Dongen J.J.M., van der Burg M.
      J. Clin. Invest. 120:1265-1274(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INVOLVEMENT IN CVID6.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "SCIMP, a transmembrane adapter protein involved in major histocompatibility complex class II signaling."
      Draber P., Vonkova I., Stepanek O., Hrdinka M., Kucova M., Skopcova T., Otahal P., Angelisova P., Horejsi V., Yeung M., Weiss A., Brdicka T.
      Mol. Cell. Biol. 31:4550-4562(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CD53 AND SCIMP.
    15. "CD81 extracellular domain 3D structure: insight into the tetraspanin superfamily structural motifs."
      Kitadokoro K., Bordo D., Galli G., Petracca R., Falugi F., Abrignani S., Grandi G., Bolognesi M.
      EMBO J. 20:12-18(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 113-202.
    16. "Subunit association and conformational flexibility in the head subdomain of human CD81 large extracellular loop."
      Kitadokoro K., Ponassi M., Galli G., Petracca R., Falugi F., Grandi G., Bolognesi M.
      Biol. Chem. 383:1447-1452(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 113-201.

    Entry informationi

    Entry nameiCD81_HUMAN
    AccessioniPrimary (citable) accession number: P60033
    Secondary accession number(s): P18582, Q5U0J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 21, 2003
    Last sequence update: November 21, 2003
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3