Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P60010

- ACT_YEAST

UniProt

P60010 - ACT_YEAST

Protein

Actin

Gene

ACT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. actomyosin contractile ring contraction Source: SGD
    2. ascospore wall assembly Source: SGD
    3. budding cell isotropic bud growth Source: SGD
    4. cellular response to oxidative stress Source: SGD
    5. chronological cell aging Source: SGD
    6. DNA repair Source: SGD
    7. endocytosis Source: SGD
    8. establishment of cell polarity Source: SGD
    9. establishment of mitotic spindle orientation Source: SGD
    10. exocytosis Source: SGD
    11. fungal-type cell wall organization Source: SGD
    12. histone acetylation Source: SGD
    13. mitochondrion inheritance Source: SGD
    14. protein secretion Source: SGD
    15. vacuole inheritance Source: SGD
    16. vesicle transport along actin filament Source: SGD

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30423-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin
    Gene namesi
    Name:ACT1
    Synonyms:ABY1, END7
    Ordered Locus Names:YFL039C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    CYGDiYFL039c.
    SGDiS000001855. ACT1.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cortical patch Source: SGD
    2. actin filament Source: SGD
    3. actin filament bundle Source: SGD
    4. cellular bud neck contractile ring Source: SGD
    5. Ino80 complex Source: SGD
    6. NuA4 histone acetyltransferase complex Source: SGD
    7. Swr1 complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375ActinPRO_0000089051Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP60010.
    PaxDbiP60010.
    PeptideAtlasiP60010.
    PRIDEiP60010.

    2D gel databases

    SWISS-2DPAGEP60010.

    Expressioni

    Gene expression databases

    GenevestigatoriP60010.

    Interactioni

    Subunit structurei

    Component of the INO80 complex. Component of the SWR1 complex. Component of the NuA4 complex.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    groLP0A6F55EBI-2169,EBI-543750From a different organism.
    HSP82P028292EBI-2169,EBI-8659
    SRV2P175555EBI-2169,EBI-4024
    SWR1Q0547112EBI-2169,EBI-22102
    TCP1P126123EBI-2169,EBI-19045

    Protein-protein interaction databases

    BioGridi31107. 696 interactions.
    DIPiDIP-310N.
    IntActiP60010. 140 interactions.
    MINTiMINT-374866.

    Structurei

    Secondary structure

    1
    375
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 125
    Beta strandi14 – 218
    Beta strandi28 – 325
    Beta strandi35 – 406
    Turni45 – 473
    Helixi56 – 605
    Helixi62 – 643
    Beta strandi65 – 684
    Beta strandi70 – 723
    Beta strandi75 – 773
    Helixi79 – 9113
    Turni92 – 943
    Helixi98 – 1003
    Beta strandi103 – 1075
    Helixi113 – 12513
    Beta strandi130 – 1367
    Helixi137 – 1448
    Beta strandi148 – 1558
    Beta strandi160 – 1667
    Helixi172 – 1743
    Beta strandi176 – 1794
    Helixi182 – 19413
    Turni195 – 1973
    Helixi203 – 21614
    Helixi223 – 2286
    Turni229 – 2313
    Beta strandi234 – 2363
    Beta strandi238 – 2414
    Beta strandi247 – 2504
    Helixi253 – 2564
    Helixi258 – 2614
    Helixi264 – 2674
    Helixi274 – 28310
    Helixi287 – 2948
    Beta strandi297 – 3015
    Helixi302 – 3043
    Helixi309 – 32012
    Turni333 – 3364
    Helixi338 – 34811
    Helixi350 – 3545
    Beta strandi356 – 3583
    Helixi359 – 3657
    Helixi367 – 3693
    Helixi370 – 3734

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YAGX-ray1.90A1-375[»]
    1YVNX-ray2.10A1-375[»]
    ProteinModelPortaliP60010.
    SMRiP60010. Positions 4-375.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP60010.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the actin family.Curated

    Phylogenomic databases

    eggNOGiCOG5277.
    GeneTreeiENSGT00710000106384.
    HOGENOMiHOG000233340.
    KOiK05692.
    OMAiSIVHLKC.
    OrthoDBiEOG75B8FT.

    Family and domain databases

    InterProiIPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR004001. Actin_CS.
    [Graphical view]
    PANTHERiPTHR11937. PTHR11937. 1 hit.
    PfamiPF00022. Actin. 1 hit.
    [Graphical view]
    PRINTSiPR00190. ACTIN.
    SMARTiSM00268. ACTIN. 1 hit.
    [Graphical view]
    PROSITEiPS00406. ACTINS_1. 1 hit.
    PS00432. ACTINS_2. 1 hit.
    PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P60010-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK    50
    DSYVGDEAQS KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE 100
    HPVLLTEAPM NPKSNREKMT QIMFETFNVP AFYVSIQAVL SLYSSGRTTG 150
    IVLDSGDGVT HVVPIYAGFS LPHAILRIDL AGRDLTDYLM KILSERGYSF 200
    STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY ELPDGQVITI 250
    GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS 300
    GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT 350
    TFQQMWISKQ EYDESGPSIV HHKCF 375
    Length:375
    Mass (Da):41,690
    Last modified:July 21, 1986 - v1
    Checksum:i87AC19B0B0BC9E71
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti178 – 1781I → L in CAA24598. (PubMed:7001447)Curated
    Sequence conflicti308 – 3081G → S in CAA24598. (PubMed:7001447)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti143 – 1431Y → F in strain: CBS 1907.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01288 Genomic DNA. Translation: CAA24597.1.
    V01289 Genomic DNA. Translation: CAA24598.1. Sequence problems.
    V01290 Genomic DNA. Translation: CAA24599.1.
    L00026 Genomic DNA. Translation: AAA34391.1.
    D50617 Genomic DNA. Translation: BAA21512.1.
    AJ389075 Genomic DNA. Translation: CAC00716.1.
    AJ389076 Genomic DNA. Translation: CAC00717.1.
    BK006940 Genomic DNA. Translation: DAA12401.1.
    PIRiA03005. ATBY.
    JS0702.
    RefSeqiNP_116614.1. NM_001179927.1.

    Genome annotation databases

    EnsemblFungiiYFL039C; YFL039C; YFL039C.
    GeneIDi850504.
    KEGGisce:YFL039C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01288 Genomic DNA. Translation: CAA24597.1 .
    V01289 Genomic DNA. Translation: CAA24598.1 . Sequence problems.
    V01290 Genomic DNA. Translation: CAA24599.1 .
    L00026 Genomic DNA. Translation: AAA34391.1 .
    D50617 Genomic DNA. Translation: BAA21512.1 .
    AJ389075 Genomic DNA. Translation: CAC00716.1 .
    AJ389076 Genomic DNA. Translation: CAC00717.1 .
    BK006940 Genomic DNA. Translation: DAA12401.1 .
    PIRi A03005. ATBY.
    JS0702.
    RefSeqi NP_116614.1. NM_001179927.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YAG X-ray 1.90 A 1-375 [» ]
    1YVN X-ray 2.10 A 1-375 [» ]
    ProteinModelPortali P60010.
    SMRi P60010. Positions 4-375.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31107. 696 interactions.
    DIPi DIP-310N.
    IntActi P60010. 140 interactions.
    MINTi MINT-374866.

    2D gel databases

    SWISS-2DPAGE P60010.

    Proteomic databases

    MaxQBi P60010.
    PaxDbi P60010.
    PeptideAtlasi P60010.
    PRIDEi P60010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFL039C ; YFL039C ; YFL039C .
    GeneIDi 850504.
    KEGGi sce:YFL039C.

    Organism-specific databases

    CYGDi YFL039c.
    SGDi S000001855. ACT1.

    Phylogenomic databases

    eggNOGi COG5277.
    GeneTreei ENSGT00710000106384.
    HOGENOMi HOG000233340.
    KOi K05692.
    OMAi SIVHLKC.
    OrthoDBi EOG75B8FT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30423-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P60010.
    NextBioi 966206.

    Gene expression databases

    Genevestigatori P60010.

    Family and domain databases

    InterProi IPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR004001. Actin_CS.
    [Graphical view ]
    PANTHERi PTHR11937. PTHR11937. 1 hit.
    Pfami PF00022. Actin. 1 hit.
    [Graphical view ]
    PRINTSi PR00190. ACTIN.
    SMARTi SM00268. ACTIN. 1 hit.
    [Graphical view ]
    PROSITEi PS00406. ACTINS_1. 1 hit.
    PS00432. ACTINS_2. 1 hit.
    PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae."
      Gallwitz D., Sures I.
      Proc. Natl. Acad. Sci. U.S.A. 77:2546-2550(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Isolation and sequence of the gene for actin in Saccharomyces cerevisiae."
      Ng R., Abelson J.
      Proc. Natl. Acad. Sci. U.S.A. 77:3912-3916(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Lariat structures are in vivo intermediates in yeast pre-mRNA splicing."
      Domdey H., Apostol B., Lin R.J., Newman A., Brody E., Abelson J.
      Cell 39:611-621(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-58.
    4. "Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae."
      Gallwitz D., Seidel R.
      Nucleic Acids Res. 8:1043-1059(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 5-57.
    5. "The nucleotide sequences of the actin genes from Saccharomyces carlsbergensis and Saccharomyces cerevisiae are identical except for their introns."
      Nellen W., Donath C., Moos M., Gallwitz D.
      J. Mol. Appl. Genet. 1:239-244(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Carlsbergensis.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    8. "Partial sequence analysis of the actin gene and its potential for studying the phylogeny of Candida species and their teleomorphs."
      Daniel H.-M., Sorrell T.C., Meyer W.
      Int. J. Syst. Evol. Microbiol. 51:1593-1606(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-358.
      Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632 and CBS 1907.
    9. "Unusual metabolism of the yeast actin amino terminus."
      Cook R.K., Sheff D.R., Rubenstein P.A.
      J. Biol. Chem. 266:16825-16833(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION AT MET-1, PROTEIN SEQUENCE OF N-TERMINUS.
    10. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
      Norbeck J., Blomberg A.
      FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-25.
      Strain: ATCC 38531 / Y41.
    11. "Site-directed mutagenesis of the yeast actin gene: a test for actin function in vivo."
      Johannes F.-Z., Gallwitz D.
      EMBO J. 10:3951-3958(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism."
      Vorobiev S., Strokopytov B., Drubin D.G., Frieden C., Ono S., Condeelis J., Rubenstein P.A., Almo S.C.
      Proc. Natl. Acad. Sci. U.S.A. 100:5760-5765(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).

    Entry informationi

    Entry nameiACT_YEAST
    AccessioniPrimary (citable) accession number: P60010
    Secondary accession number(s): D6VTJ1
    , P02579, Q9P3X6, Q9P3X7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3