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Protein

Actin

Gene

ACT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

GO - Molecular functioni

  • ATP binding Source: WormBase
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • actomyosin contractile ring contraction Source: SGD
  • ascospore wall assembly Source: SGD
  • budding cell isotropic bud growth Source: SGD
  • cellular response to oxidative stress Source: SGD
  • chronological cell aging Source: SGD
  • DNA repair Source: SGD
  • endocytosis Source: SGD
  • establishment of cell polarity Source: SGD
  • establishment of mitotic spindle orientation Source: SGD
  • exocytosis Source: SGD
  • fungal-type cell wall organization Source: SGD
  • histone acetylation Source: SGD
  • mitochondrion inheritance Source: SGD
  • protein secretion Source: SGD
  • vacuole inheritance Source: SGD
  • vesicle transport along actin filament Source: SGD
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30423-MONOMER.
ReactomeiR-SCE-5626467. RHO GTPases activate IQGAPs.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin
Gene namesi
Name:ACT1
Synonyms:ABY1, END7
Ordered Locus Names:YFL039C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL039C.
SGDiS000001855. ACT1.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • actin filament Source: SGD
  • actin filament bundle Source: SGD
  • cellular bud neck contractile ring Source: SGD
  • Ino80 complex Source: SGD
  • NuA4 histone acetyltransferase complex Source: SGD
  • Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000890511 – 375ActinAdd BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Cross-linki191Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP60010.
PRIDEiP60010.

2D gel databases

SWISS-2DPAGEP60010.

PTM databases

iPTMnetiP60010.

Interactioni

Subunit structurei

Interacts with YIH1 (via C-terminus); this interaction occurs in a GCN1-independent manner (PubMed:15126500, PubMed:21239490). Component of the INO80 complex. Component of the SWR1 complex. Component of the NuA4 complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F55EBI-2169,EBI-543750From a different organism.
HSP82P028292EBI-2169,EBI-8659
SRV2P175555EBI-2169,EBI-4024
SWR1Q0547112EBI-2169,EBI-22102
TCP1P126123EBI-2169,EBI-19045

Protein-protein interaction databases

BioGridi31107. 702 interactors.
DIPiDIP-310N.
IntActiP60010. 147 interactors.
MINTiMINT-374866.

Structurei

Secondary structure

1375
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Beta strandi14 – 21Combined sources8
Beta strandi28 – 32Combined sources5
Beta strandi35 – 40Combined sources6
Turni45 – 47Combined sources3
Helixi56 – 60Combined sources5
Helixi62 – 64Combined sources3
Beta strandi65 – 68Combined sources4
Beta strandi70 – 72Combined sources3
Beta strandi75 – 77Combined sources3
Helixi79 – 91Combined sources13
Turni92 – 94Combined sources3
Helixi98 – 100Combined sources3
Beta strandi103 – 107Combined sources5
Helixi113 – 125Combined sources13
Beta strandi130 – 136Combined sources7
Helixi137 – 144Combined sources8
Beta strandi148 – 155Combined sources8
Beta strandi160 – 166Combined sources7
Helixi172 – 174Combined sources3
Beta strandi176 – 179Combined sources4
Helixi182 – 194Combined sources13
Turni195 – 197Combined sources3
Helixi203 – 216Combined sources14
Helixi223 – 228Combined sources6
Turni229 – 231Combined sources3
Beta strandi234 – 236Combined sources3
Beta strandi238 – 241Combined sources4
Beta strandi247 – 250Combined sources4
Helixi253 – 256Combined sources4
Helixi258 – 261Combined sources4
Helixi264 – 267Combined sources4
Helixi274 – 283Combined sources10
Helixi287 – 294Combined sources8
Beta strandi297 – 301Combined sources5
Helixi302 – 304Combined sources3
Helixi309 – 320Combined sources12
Turni333 – 336Combined sources4
Helixi338 – 348Combined sources11
Helixi350 – 354Combined sources5
Beta strandi356 – 358Combined sources3
Helixi359 – 365Combined sources7
Helixi367 – 369Combined sources3
Helixi370 – 373Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YAGX-ray1.90A1-375[»]
1YVNX-ray2.10A1-375[»]
ProteinModelPortaliP60010.
SMRiP60010.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP60010.

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118957.
HOGENOMiHOG000233340.
InParanoidiP60010.
KOiK05692.
OMAiPFHTTAE.
OrthoDBiEOG092C2KFK.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P60010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK
60 70 80 90 100
DSYVGDEAQS KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
110 120 130 140 150
HPVLLTEAPM NPKSNREKMT QIMFETFNVP AFYVSIQAVL SLYSSGRTTG
160 170 180 190 200
IVLDSGDGVT HVVPIYAGFS LPHAILRIDL AGRDLTDYLM KILSERGYSF
210 220 230 240 250
STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY ELPDGQVITI
260 270 280 290 300
GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS
310 320 330 340 350
GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT
360 370
TFQQMWISKQ EYDESGPSIV HHKCF
Length:375
Mass (Da):41,690
Last modified:July 21, 1986 - v1
Checksum:i87AC19B0B0BC9E71
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178I → L in CAA24598 (PubMed:7001447).Curated1
Sequence conflicti308G → S in CAA24598 (PubMed:7001447).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti143Y → F in strain: CBS 1907. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01288 Genomic DNA. Translation: CAA24597.1.
V01289 Genomic DNA. Translation: CAA24598.1. Sequence problems.
V01290 Genomic DNA. Translation: CAA24599.1.
L00026 Genomic DNA. Translation: AAA34391.1.
D50617 Genomic DNA. Translation: BAA21512.1.
AJ389075 Genomic DNA. Translation: CAC00716.1.
AJ389076 Genomic DNA. Translation: CAC00717.1.
BK006940 Genomic DNA. Translation: DAA12401.1.
PIRiA03005. ATBY.
JS0702.
RefSeqiNP_116614.1. NM_001179927.1.

Genome annotation databases

EnsemblFungiiBAA21512; BAA21512; BAA21512.
YFL039C; YFL039C; YFL039C.
GeneIDi850504.
KEGGisce:YFL039C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01288 Genomic DNA. Translation: CAA24597.1.
V01289 Genomic DNA. Translation: CAA24598.1. Sequence problems.
V01290 Genomic DNA. Translation: CAA24599.1.
L00026 Genomic DNA. Translation: AAA34391.1.
D50617 Genomic DNA. Translation: BAA21512.1.
AJ389075 Genomic DNA. Translation: CAC00716.1.
AJ389076 Genomic DNA. Translation: CAC00717.1.
BK006940 Genomic DNA. Translation: DAA12401.1.
PIRiA03005. ATBY.
JS0702.
RefSeqiNP_116614.1. NM_001179927.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YAGX-ray1.90A1-375[»]
1YVNX-ray2.10A1-375[»]
ProteinModelPortaliP60010.
SMRiP60010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31107. 702 interactors.
DIPiDIP-310N.
IntActiP60010. 147 interactors.
MINTiMINT-374866.

PTM databases

iPTMnetiP60010.

2D gel databases

SWISS-2DPAGEP60010.

Proteomic databases

MaxQBiP60010.
PRIDEiP60010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA21512; BAA21512; BAA21512.
YFL039C; YFL039C; YFL039C.
GeneIDi850504.
KEGGisce:YFL039C.

Organism-specific databases

EuPathDBiFungiDB:YFL039C.
SGDiS000001855. ACT1.

Phylogenomic databases

GeneTreeiENSGT00760000118957.
HOGENOMiHOG000233340.
InParanoidiP60010.
KOiK05692.
OMAiPFHTTAE.
OrthoDBiEOG092C2KFK.

Enzyme and pathway databases

BioCyciYEAST:G3O-30423-MONOMER.
ReactomeiR-SCE-5626467. RHO GTPases activate IQGAPs.

Miscellaneous databases

EvolutionaryTraceiP60010.
PROiP60010.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACT_YEAST
AccessioniPrimary (citable) accession number: P60010
Secondary accession number(s): D6VTJ1
, P02579, Q9P3X6, Q9P3X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.