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Protein

Anaphase-promoting complex subunit 15

Gene

ANAPC15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. In the complex, plays a role in the release of the mitotic checkpoint complex (MCC) from the APC/C: not required for APC/C activity itself, but promotes the turnover of CDC20 and MCC on the APC/C, thereby participating in the responsiveness of the spindle assembly checkpoint. Also required for degradation of CDC20.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-HSA-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-HSA-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-HSA-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-HSA-176412. Phosphorylation of the APC/C.
R-HSA-179409. APC-Cdc20 mediated degradation of Nek2A.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2559582. Senescence-Associated Secretory Phenotype (SASP).

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 15
Short name:
APC15
Gene namesi
Name:ANAPC15
Synonyms:C11orf51
ORF Names:HSPC020
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:24531. ANAPC15.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: UniProtKB
  • cytosol Source: Reactome
  • intracellular Source: LIFEdb
  • nucleoplasm Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485346.

Polymorphism and mutation databases

BioMutaiANAPC15.
DMDMi38372627.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Anaphase-promoting complex subunit 15PRO_0000084072Add
BLAST

Proteomic databases

EPDiP60006.
MaxQBiP60006.
PaxDbiP60006.
PeptideAtlasiP60006.
PRIDEiP60006.

Expressioni

Gene expression databases

BgeeiP60006.
CleanExiHS_C11orf51.
ExpressionAtlasiP60006. baseline and differential.
GenevisibleiP60006. HS.

Organism-specific databases

HPAiHPA036596.

Interactioni

Subunit structurei

The mammalian APC/C is composed of 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa.2 Publications

Protein-protein interaction databases

BioGridi117412. 48 interactions.
DIPiDIP-61021N.
IntActiP60006. 44 interactions.
MINTiMINT-4711804.
STRINGi9606.ENSP00000227618.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UI9electron microscopy3.60D1-121[»]
5A31electron microscopy4.30D6-121[»]
5G04electron microscopy4.00D1-121[»]
5G05electron microscopy3.40D1-121[»]
ProteinModelPortaliP60006.
SMRiP60006. Positions 2-56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 11755Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the APC15 family.Curated

Phylogenomic databases

eggNOGiENOG410J0F5. Eukaryota.
ENOG410Y01T. LUCA.
GeneTreeiENSGT00390000000938.
HOGENOMiHOG000026787.
HOVERGENiHBG002951.
InParanoidiP60006.
OMAiQTWLQSI.
PhylomeDBiP60006.

Family and domain databases

InterProiIPR026182. ANAPC15.
[Graphical view]
PANTHERiPTHR22526. PTHR22526. 1 hit.
PfamiPF15243. ANAPC15. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P60006-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTLFPSLFP RVTETLWFNL DRPCVEETEL QQQEQQHQAW LQSIAEKDNN
60 70 80 90 100
LVPIGKPASE HYDDEEEEDD EDDEDSEEDS EDDEDMQDMD EMNDYNESPD
110 120
DGEVNEVDME GNEQDQDQWM I
Length:121
Mass (Da):14,281
Last modified:November 14, 2003 - v1
Checksum:i2C889607C3013A82
GO
Isoform 2 (identifier: P60006-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     106-106: E → EPPLQ

Note: No experimental confimation available.
Show »
Length:125
Mass (Da):14,716
Checksum:iBCDB1E98984878D3
GO

Sequence cautioni

The sequence EAW74842.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731D → G in AK222622 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei106 – 1061E → EPPLQ in isoform 2. 1 PublicationVSP_055048

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077206 mRNA. Translation: AAD27001.1.
AL080071 mRNA. Translation: CAB45698.1.
AK222622 mRNA. No translation available.
AP000812 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74842.1. Sequence problems.
BC005156 mRNA. Translation: AAH05156.1.
BC005393 mRNA. Translation: AAH05393.1.
CCDSiCCDS60880.1. [P60006-2]
CCDS8210.1. [P60006-1]
PIRiT12467.
RefSeqiNP_001265414.1. NM_001278485.1. [P60006-2]
NP_001265415.1. NM_001278486.1. [P60006-2]
NP_001265416.1. NM_001278487.1. [P60006-1]
NP_001265417.1. NM_001278488.1. [P60006-1]
NP_001265418.1. NM_001278489.1. [P60006-1]
NP_001265419.1. NM_001278490.1. [P60006-1]
NP_001265420.1. NM_001278491.1. [P60006-1]
NP_001265421.1. NM_001278492.1. [P60006-1]
NP_001265422.1. NM_001278493.1. [P60006-1]
NP_001265423.1. NM_001278494.1. [P60006-1]
NP_054761.1. NM_014042.2. [P60006-1]
UniGeneiHs.38044.

Genome annotation databases

EnsembliENST00000227618; ENSP00000227618; ENSG00000110200. [P60006-1]
ENST00000535234; ENSP00000446412; ENSG00000110200. [P60006-1]
ENST00000535503; ENSP00000443383; ENSG00000110200. [P60006-1]
ENST00000538393; ENSP00000440002; ENSG00000110200. [P60006-1]
ENST00000538919; ENSP00000440983; ENSG00000110200. [P60006-1]
ENST00000542531; ENSP00000443911; ENSG00000110200. [P60006-1]
ENST00000543587; ENSP00000439403; ENSG00000110200. [P60006-2]
ENST00000545680; ENSP00000443645; ENSG00000110200. [P60006-2]
ENST00000545944; ENSP00000446383; ENSG00000110200. [P60006-1]
GeneIDi25906.
KEGGihsa:25906.
UCSCiuc001orv.5. human. [P60006-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077206 mRNA. Translation: AAD27001.1.
AL080071 mRNA. Translation: CAB45698.1.
AK222622 mRNA. No translation available.
AP000812 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74842.1. Sequence problems.
BC005156 mRNA. Translation: AAH05156.1.
BC005393 mRNA. Translation: AAH05393.1.
CCDSiCCDS60880.1. [P60006-2]
CCDS8210.1. [P60006-1]
PIRiT12467.
RefSeqiNP_001265414.1. NM_001278485.1. [P60006-2]
NP_001265415.1. NM_001278486.1. [P60006-2]
NP_001265416.1. NM_001278487.1. [P60006-1]
NP_001265417.1. NM_001278488.1. [P60006-1]
NP_001265418.1. NM_001278489.1. [P60006-1]
NP_001265419.1. NM_001278490.1. [P60006-1]
NP_001265420.1. NM_001278491.1. [P60006-1]
NP_001265421.1. NM_001278492.1. [P60006-1]
NP_001265422.1. NM_001278493.1. [P60006-1]
NP_001265423.1. NM_001278494.1. [P60006-1]
NP_054761.1. NM_014042.2. [P60006-1]
UniGeneiHs.38044.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4UI9electron microscopy3.60D1-121[»]
5A31electron microscopy4.30D6-121[»]
5G04electron microscopy4.00D1-121[»]
5G05electron microscopy3.40D1-121[»]
ProteinModelPortaliP60006.
SMRiP60006. Positions 2-56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117412. 48 interactions.
DIPiDIP-61021N.
IntActiP60006. 44 interactions.
MINTiMINT-4711804.
STRINGi9606.ENSP00000227618.

Polymorphism and mutation databases

BioMutaiANAPC15.
DMDMi38372627.

Proteomic databases

EPDiP60006.
MaxQBiP60006.
PaxDbiP60006.
PeptideAtlasiP60006.
PRIDEiP60006.

Protocols and materials databases

DNASUi25906.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000227618; ENSP00000227618; ENSG00000110200. [P60006-1]
ENST00000535234; ENSP00000446412; ENSG00000110200. [P60006-1]
ENST00000535503; ENSP00000443383; ENSG00000110200. [P60006-1]
ENST00000538393; ENSP00000440002; ENSG00000110200. [P60006-1]
ENST00000538919; ENSP00000440983; ENSG00000110200. [P60006-1]
ENST00000542531; ENSP00000443911; ENSG00000110200. [P60006-1]
ENST00000543587; ENSP00000439403; ENSG00000110200. [P60006-2]
ENST00000545680; ENSP00000443645; ENSG00000110200. [P60006-2]
ENST00000545944; ENSP00000446383; ENSG00000110200. [P60006-1]
GeneIDi25906.
KEGGihsa:25906.
UCSCiuc001orv.5. human. [P60006-1]

Organism-specific databases

CTDi25906.
GeneCardsiANAPC15.
H-InvDBHIX0009906.
HGNCiHGNC:24531. ANAPC15.
HPAiHPA036596.
MIMi614717. gene.
neXtProtiNX_P60006.
PharmGKBiPA143485346.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J0F5. Eukaryota.
ENOG410Y01T. LUCA.
GeneTreeiENSGT00390000000938.
HOGENOMiHOG000026787.
HOVERGENiHBG002951.
InParanoidiP60006.
OMAiQTWLQSI.
PhylomeDBiP60006.

Enzyme and pathway databases

ReactomeiR-HSA-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-HSA-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-HSA-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-HSA-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-HSA-176412. Phosphorylation of the APC/C.
R-HSA-179409. APC-Cdc20 mediated degradation of Nek2A.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2559582. Senescence-Associated Secretory Phenotype (SASP).

Miscellaneous databases

GenomeRNAii25906.
PROiP60006.
SOURCEiSearch...

Gene expression databases

BgeeiP60006.
CleanExiHS_C11orf51.
ExpressionAtlasiP60006. baseline and differential.
GenevisibleiP60006. HS.

Family and domain databases

InterProiIPR026182. ANAPC15.
[Graphical view]
PANTHERiPTHR22526. PTHR22526. 1 hit.
PfamiPF15243. ANAPC15. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Umbilical cord blood.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Smooth muscle.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney and Muscle.
  7. "APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment."
    Mansfeld J., Collin P., Collins M.O., Choudhary J.S., Pines J.
    Nat. Cell Biol. 13:1234-1243(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE APC/C COMPLEX.
  8. "Molecular architecture and mechanism of the anaphase-promoting complex."
    Chang L., Zhang Z., Yang J., McLaughlin S.H., Barford D.
    Nature 513:388-393(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.4 ANGSTROMS) OF THE APC/C, SUBUNIT.

Entry informationi

Entry nameiAPC15_HUMAN
AccessioniPrimary (citable) accession number: P60006
Secondary accession number(s): G3V1Q3, Q9CXK2, Q9Y269
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: July 6, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.