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P59998 (ARPC4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-related protein 2/3 complex subunit 4
Alternative name(s):
Arp2/3 complex 20 kDa subunit
Short name=p20-ARC
Gene names
Name:ARPC4
Synonyms:ARC20
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length168 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.

Subunit structure

Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

Subcellular location

Cytoplasmcytoskeleton. Cell projection Ref.1.

Sequence similarities

Belongs to the ARPC4 family.

Sequence caution

The sequence AAH12596.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P59998-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P59998-2)

The sequence of this isoform differs from the canonical sequence as follows:
     110-168: EGYDISFLIT...IVAEEFLKNF → EQKKIFTIQG...LRPCRPQARP
Note: No experimental confirmation available.
Isoform 3 (identifier: P59998-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVREPGPRPGTPGCSASGQW
Note: No experimental confirmation available.
Isoform 4 (identifier: P59998-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-90: Missing.
Note: No experimental confirmation available. Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 168167Actin-related protein 2/3 complex subunit 4
PRO_0000124049

Amino acid modifications

Modified residue21N-acetylthreonine Ref.6

Natural variations

Alternative sequence1 – 9090Missing in isoform 4.
VSP_046753
Alternative sequence11M → MVREPGPRPGTPGCSASGQW in isoform 3.
VSP_046150
Alternative sequence110 – 16859EGYDI…FLKNF → EQKKIFTIQGCYPVIRCLLR RRGWVEKKMVHRSGPTLLPP QKDLDSSAMGDSDTTEDEDE DEDEEFQPSQLFDFDDLLKF DDLDGTHALMVGLCLNLRNL PWFDEVDANSFFPRCYCLGA EDDKKAFIGDKQPKKQEKNP VLVSPEFVDEALCACEEYLS NLAHMDIDKDLEAPLYLTPE GWSLFLQRYYQVVHEGAELR HLDTQVQRCEDILQQLQAVV PQIDMEGDRNIWIVKPGAKS RGRGIMCMDHLEEMLKLVNG NPVVMKDGKWVVQKYIERPL LIFGTKFDLRQWFLVTDWNP LTVWFYRDSYIRFSTQPFSL KNLDNSVHLCNNSIQKHLEN SCHRHPLLPPDNMWSSQRFQ AHLQEMGAPNAWSTIIVPGM KDAVIHALQTSQDTVQCRKA SFELYGADFVFGEDFQPWLI EINASPTMAPSTAVTARLCA GVQADTLRVVIDRMLDRNCD TGAFELIYKQPVTTSPASTP RPSCLLPMYSDTRARSSDDS TASWWALRPCRPQARP in isoform 2.
VSP_046151

Experimental info

Sequence conflict941M → T in AAH12596. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 273CCB230AC703DF

FASTA16819,667
        10         20         30         40         50         60 
MTATLRPYLS AVRATLQAAL CLENFSSQVV ERHNKPEVEV RSSKELLLQP VTISRNEKEK 

        70         80         90        100        110        120 
VLIEGSINSV RVSIAVKQAD EIEKILCHKF MRFMMMRAEN FFILRRKPVE GYDISFLITN 

       130        140        150        160 
FHTEQMYKHK LVDFVIHFME EIDKEISEMK LSVNARARIV AEEFLKNF 

« Hide

Isoform 2 [UniParc].

Checksum: E05F7DC236DF9674
Show »

FASTA62571,719
Isoform 3 [UniParc].

Checksum: F61DD748D01B8880
Show »

FASTA18721,588
Isoform 4 [UniParc].

Checksum: 32DD605A770B6706
Show »

FASTA789,552

References

« Hide 'large scale' references
[1]"Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins."
Machesky L.M., Reeves E., Wientjes F., Mattheyse F.J., Grogan A., Totty N.F., Burlingame A.L., Hsuan J.J., Segal A.W.
Biochem. J. 328:105-112(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
[2]"The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly."
Welch M.D., Depace A.H., Verma S., Iwamatsu A., Mitchison T.J.
J. Cell Biol. 138:375-384(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Fetal brain.
[4]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-168 (ISOFORM 1).
Tissue: Choriocarcinoma and Prostate.
[6]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13, ACETYLATION AT THR-2.
Tissue: Platelet.
[7]"Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity."
Gournier H., Goley E.D., Niederstrasser H., Trinh T., Welch M.D.
Mol. Cell 8:1041-1052(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: ACTIN-BINDING, RECONSTITUTION OF THE ARP2/3 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019888 mRNA. Translation: AAB71548.1.
AF006087 mRNA. Translation: AAB64192.1.
BX419672 mRNA. No translation available.
AC022382 Genomic DNA. No translation available.
BC012596 mRNA. Translation: AAH12596.3. Different initiation.
BC025289 mRNA. No translation available.
CCDSCCDS43047.1. [P59998-1]
CCDS46743.1. [P59998-4]
CCDS56238.1. [P59998-3]
RefSeqNP_001020130.1. NM_001024959.2. [P59998-4]
NP_001020131.1. NM_001024960.2. [P59998-4]
NP_001185709.1. NM_001198780.1. [P59998-3]
NP_001185722.1. NM_001198793.1. [P59998-2]
NP_005709.1. NM_005718.4. [P59998-1]
UniGeneHs.323342.

3D structure databases

ProteinModelPortalP59998.
SMRP59998. Positions 2-168.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115400. 41 interactions.
DIPDIP-33188N.
IntActP59998. 9 interactions.
MINTMINT-5000208.
STRING9606.ENSP00000380431.

PTM databases

PhosphoSiteP59998.

Polymorphism databases

DMDM38372625.

Proteomic databases

MaxQBP59998.
PaxDbP59998.
PRIDEP59998.

Protocols and materials databases

DNASU10093.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000287613; ENSP00000287613; ENSG00000241553. [P59998-4]
ENST00000397261; ENSP00000380431; ENSG00000241553. [P59998-1]
ENST00000433034; ENSP00000388169; ENSG00000241553. [P59998-3]
ENST00000498623; ENSP00000432235; ENSG00000241553. [P59998-4]
GeneID100526693.
10093.
KEGGhsa:100526693.
hsa:10093.
UCSCuc003bsz.2. human. [P59998-1]

Organism-specific databases

CTD100526693.
10093.
GeneCardsGC03P009834.
HGNCHGNC:707. ARPC4.
MIM604226. gene.
neXtProtNX_P59998.
PharmGKBPA25002.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG261545.
HOGENOMHOG000202303.
HOVERGENHBG050582.
KOK05755.
OMAMLKHKIV.
OrthoDBEOG7VTDPM.
PhylomeDBP59998.
TreeFamTF105621.
TF313087.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP59998.
BgeeP59998.
CleanExHS_ARPC4.
GenevestigatorP59998.

Family and domain databases

InterProIPR008384. ARPC4.
[Graphical view]
PANTHERPTHR22629. PTHR22629. 1 hit.
PfamPF05856. ARPC4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARPC4. human.
GeneWikiARPC4.
NextBio34054911.
PROP59998.
SOURCESearch...

Entry information

Entry nameARPC4_HUMAN
AccessionPrimary (citable) accession number: P59998
Secondary accession number(s): C9JWM7 expand/collapse secondary AC list , E7ETI0, F6TTL5, O15509, Q6P0W5, Q96QJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM