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P59966

- DNAB_MYCBO

UniProt

P59966 - DNAB_MYCBO

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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity
The intein is an endonuclease.Curated

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi226 – 2338ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. endonuclease activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Mbo dnaB intein
Gene namesi
Name:dnaB
Ordered Locus Names:Mb0059
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Replicative DNA helicase, 1st partBy similarityPRO_0000013276Add
BLAST
Chaini400 – 815416Endonuclease PI-MboHIPPRO_0000013277Add
BLAST
Chaini816 – 87459Replicative DNA helicase, 2nd partBy similarityPRO_0000013278Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb0059.

Structurei

3D structure databases

ProteinModelPortaliP59966.
SMRiP59966. Positions 23-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 462268SF4 helicase; first partPROSITE-ProRule annotationAdd
BLAST
Domaini582 – 66079DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST
Domaini612 – 874263SF4 helicase; second partPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0305.
HOGENOMiHOG000113195.
KOiK02314.
OMAiIAVHNSI.
OrthoDBiEOG6T4RW5.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59966-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE
60 70 80 90 100
RLRPGDFYRP AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA
110 120 130 140 150
PYLHTLISTV PTAANAGYYA SIVAEKALLR RLVEAGTRVV QYGYAGAEGA
160 170 180 190 200
DVAEVVDRAQ AEIYDVADRR LSEDFVALED LLQPTMDEID AIASSGGLAR
210 220 230 240 250
GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM RSCSIRHRMA
260 270 280 290 300
SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS
310 320 330 340 350
EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR
360 370 380 390 400
QVEVSEFSRH LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC
410 420 430 440 450
LTASTRILRA DTGAEVAFGE LMRSGERPMV WSLDERLRMV ARPMINVFPS
460 470 480 490 500
GRKEVFRLRL ASGREVEATG SHPFMKFEGW TPLAQLKVGD RIAAPRRVPE
510 520 530 540 550
PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL AAVTVSAAHS
560 570 580 590 600
DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC
610 620 630 640 650
VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD
660 670 680 690 700
VAQLLLRVGI FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA
710 720 730 740 750
VAAQEMLRQL KGPVRNPNLD SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW
760 770 780 790 800
KHSPSRSRPH RAEARIEDRA IHELARGDAY WDTVVEITSI GDQHVFDGTV
810 820 830 840 850
SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG GEADFILAKH
860 870
RNGPTKTVTV AHQLHLSRFA NMAR
Length:874
Mass (Da):96,818
Last modified:October 31, 2003 - v1
Checksum:i3CFA596E1C7CE7CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO41292.1.
RefSeqiNP_853728.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD92921; CAD92921; Mb0059.
GeneIDi1094330.
KEGGimbo:Mb0059.
PATRICi18001794. VBIMycBov88188_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO41292.1 .
RefSeqi NP_853728.1. NC_002945.3.

3D structure databases

ProteinModelPortali P59966.
SMRi P59966. Positions 23-167.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb0059.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD92921 ; CAD92921 ; Mb0059 .
GeneIDi 1094330.
KEGGi mbo:Mb0059.
PATRICi 18001794. VBIMycBov88188_0065.

Phylogenomic databases

eggNOGi COG0305.
HOGENOMi HOG000113195.
KOi K02314.
OMAi IAVHNSI.
OrthoDBi EOG6T4RW5.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiDNAB_MYCBO
AccessioniPrimary (citable) accession number: P59966
Secondary accession number(s): X2BDX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3