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P59966

- DNAB_MYCBO

UniProt

P59966 - DNAB_MYCBO

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Protein
Replicative DNA helicase
Gene
dnaB, Mb0059
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.
The intein is an endonuclease Reviewed prediction.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi226 – 2338ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. endonuclease activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Mbo dnaB intein
Gene namesi
Name:dnaB
Ordered Locus Names:Mb0059
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Replicative DNA helicase, 1st part By similarity
PRO_0000013276Add
BLAST
Chaini400 – 815416Endonuclease PI-MboHIP
PRO_0000013277Add
BLAST
Chaini816 – 87459Replicative DNA helicase, 2nd part By similarity
PRO_0000013278Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb0059.

Structurei

3D structure databases

ProteinModelPortaliP59966.
SMRiP59966. Positions 23-167.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 462268SF4 helicase; first part
Add
BLAST
Domaini582 – 66079DOD-type homing endonuclease
Add
BLAST
Domaini612 – 874263SF4 helicase; second part
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0305.
HOGENOMiHOG000113195.
KOiK02314.
OMAiIAVHNSI.
OrthoDBiEOG6T4RW5.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59966-1 [UniParc]FASTAAdd to Basket

« Hide

MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE    50
RLRPGDFYRP AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA 100
PYLHTLISTV PTAANAGYYA SIVAEKALLR RLVEAGTRVV QYGYAGAEGA 150
DVAEVVDRAQ AEIYDVADRR LSEDFVALED LLQPTMDEID AIASSGGLAR 200
GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM RSCSIRHRMA 250
SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 300
EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR 350
QVEVSEFSRH LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC 400
LTASTRILRA DTGAEVAFGE LMRSGERPMV WSLDERLRMV ARPMINVFPS 450
GRKEVFRLRL ASGREVEATG SHPFMKFEGW TPLAQLKVGD RIAAPRRVPE 500
PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL AAVTVSAAHS 550
DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 600
VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD 650
VAQLLLRVGI FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA 700
VAAQEMLRQL KGPVRNPNLD SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW 750
KHSPSRSRPH RAEARIEDRA IHELARGDAY WDTVVEITSI GDQHVFDGTV 800
SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG GEADFILAKH 850
RNGPTKTVTV AHQLHLSRFA NMAR 874
Length:874
Mass (Da):96,818
Last modified:October 31, 2003 - v1
Checksum:i3CFA596E1C7CE7CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO41292.1.
RefSeqiNP_853728.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD92921; CAD92921; Mb0059.
GeneIDi1094330.
KEGGimbo:Mb0059.
PATRICi18001794. VBIMycBov88188_0065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO41292.1 .
RefSeqi NP_853728.1. NC_002945.3.

3D structure databases

ProteinModelPortali P59966.
SMRi P59966. Positions 23-167.
ModBasei Search...

Protein-protein interaction databases

STRINGi 233413.Mb0059.

Protein family/group databases

MEROPSi N10.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD92921 ; CAD92921 ; Mb0059 .
GeneIDi 1094330.
KEGGi mbo:Mb0059.
PATRICi 18001794. VBIMycBov88188_0065.

Phylogenomic databases

eggNOGi COG0305.
HOGENOMi HOG000113195.
KOi K02314.
OMAi IAVHNSI.
OrthoDBi EOG6T4RW5.

Family and domain databases

Gene3Di 1.10.860.10. 1 hit.
2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF48024. SSF48024. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiDNAB_MYCBO
AccessioniPrimary (citable) accession number: P59966
Secondary accession number(s): X2BDX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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