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P59966 (DNAB_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicative DNA helicase

EC=3.6.4.12

Cleaved into the following chain:

  1. Endonuclease PI-MboHIP
    EC=3.1.-.-
    Alternative name(s):
    Mbo dnaB intein
Gene names
Name:dnaB
Ordered Locus Names:Mb0059
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.

The intein is an endonuclease Potential.

Catalytic activity

ATP + H2O = ADP + phosphate.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Sequence similarities

Belongs to the helicase family. DnaB subfamily.

Contains 1 DOD-type homing endonuclease domain.

Contains 1 SF4 helicase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399Replicative DNA helicase, 1st part By similarity
PRO_0000013276
Chain400 – 815416Endonuclease PI-MboHIP
PRO_0000013277
Chain816 – 87459Replicative DNA helicase, 2nd part By similarity
PRO_0000013278

Regions

Domain195 – 462268SF4 helicase; first part
Domain582 – 66079DOD-type homing endonuclease
Domain612 – 874263SF4 helicase; second part
Nucleotide binding226 – 2338ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P59966 [UniParc].

Last modified October 31, 2003. Version 1.
Checksum: 3CFA596E1C7CE7CC

FASTA87496,818
        10         20         30         40         50         60 
MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE RLRPGDFYRP 

        70         80         90        100        110        120 
AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA PYLHTLISTV PTAANAGYYA 

       130        140        150        160        170        180 
SIVAEKALLR RLVEAGTRVV QYGYAGAEGA DVAEVVDRAQ AEIYDVADRR LSEDFVALED 

       190        200        210        220        230        240 
LLQPTMDEID AIASSGGLAR GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM 

       250        260        270        280        290        300 
RSCSIRHRMA SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 

       310        320        330        340        350        360 
EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR QVEVSEFSRH 

       370        380        390        400        410        420 
LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC LTASTRILRA DTGAEVAFGE 

       430        440        450        460        470        480 
LMRSGERPMV WSLDERLRMV ARPMINVFPS GRKEVFRLRL ASGREVEATG SHPFMKFEGW 

       490        500        510        520        530        540 
TPLAQLKVGD RIAAPRRVPE PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL 

       550        560        570        580        590        600 
AAVTVSAAHS DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 

       610        620        630        640        650        660 
VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD VAQLLLRVGI 

       670        680        690        700        710        720 
FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA VAAQEMLRQL KGPVRNPNLD 

       730        740        750        760        770        780 
SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW KHSPSRSRPH RAEARIEDRA IHELARGDAY 

       790        800        810        820        830        840 
WDTVVEITSI GDQHVFDGTV SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG 

       850        860        870 
GEADFILAKH RNGPTKTVTV AHQLHLSRFA NMAR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248334 Genomic DNA. Translation: CAD92921.1.
RefSeqNP_853728.1. NC_002945.3.

3D structure databases

ProteinModelPortalP59966.
SMRP59966. Positions 23-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb0059.

Protein family/group databases

MEROPSN10.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD92921; CAD92921; Mb0059.
GeneID1094330.
KEGGmbo:Mb0059.
PATRIC18001794. VBIMycBov88188_0065.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0305.
HOGENOMHOG000113195.
KOK02314.
OMAIAVHNSI.
OrthoDBEOG6T4RW5.
ProtClustDBPRK07773.

Family and domain databases

Gene3D1.10.860.10. 1 hit.
3.10.28.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR00665. DnaB. 1 hit.
TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAB_MYCBO
AccessionPrimary (citable) accession number: P59966
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries