Replicative DNA helicase - Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

Endonuclease PI-MboHIP
EC=3.1.-.-
Alternative name(s):
Mbo dnaB intein

Gene names

Name:	dnaB
Ordered Locus Names:	Mb0059

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity. The intein is an endonuclease Potential.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Intron homing
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 399

399

Replicative DNA helicase, 1st part By similarity

PRO_0000013276

Chain

400 – 815

416

Endonuclease PI-MboHIP

PRO_0000013277

Chain

816 – 874

59

Replicative DNA helicase, 2nd part By similarity

PRO_0000013278

Regions

Domain

195 – 462

268

SF4 helicase; first part

Domain

582 – 660

79

DOD-type homing endonuclease

Domain

612 – 874

263

SF4 helicase; second part

Nucleotide binding

226 – 233

8

ATP Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P59966 [UniParc].

Last modified October 31, 2003. Version 1.
Checksum: 3CFA596E1C7CE7CC
Show »

FASTA

874

96,818

        10         20         30         40         50         60 
MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE RLRPGDFYRP 

        70         80         90        100        110        120 
AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA PYLHTLISTV PTAANAGYYA 

       130        140        150        160        170        180 
SIVAEKALLR RLVEAGTRVV QYGYAGAEGA DVAEVVDRAQ AEIYDVADRR LSEDFVALED 

       190        200        210        220        230        240 
LLQPTMDEID AIASSGGLAR GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM 

       250        260        270        280        290        300 
RSCSIRHRMA SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 

       310        320        330        340        350        360 
EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR QVEVSEFSRH 

       370        380        390        400        410        420 
LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC LTASTRILRA DTGAEVAFGE 

       430        440        450        460        470        480 
LMRSGERPMV WSLDERLRMV ARPMINVFPS GRKEVFRLRL ASGREVEATG SHPFMKFEGW 

       490        500        510        520        530        540 
TPLAQLKVGD RIAAPRRVPE PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL 

       550        560        570        580        590        600 
AAVTVSAAHS DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 

       610        620        630        640        650        660 
VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD VAQLLLRVGI 

       670        680        690        700        710        720 
FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA VAAQEMLRQL KGPVRNPNLD 

       730        740        750        760        770        780 
SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW KHSPSRSRPH RAEARIEDRA IHELARGDAY 

       790        800        810        820        830        840 
WDTVVEITSI GDQHVFDGTV SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG 

       850        860        870 
GEADFILAKH RNGPTKTVTV AHQLHLSRFA NMAR

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References

[1]

"The complete genome sequence of Mycobacterium bovis."
Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.

Hewinson R.G.
Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248334 Genomic DNA. Translation: CAD92921.1.
RefSeq	NP_853728.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P59966.
SMR	P59966. Positions 23-167.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb0059.
Protein family/group databases
MEROPS	N10.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD92921; CAD92921; Mb0059.
GeneID	1094330.
KEGG	mbo:Mb0059.
PATRIC	18001794. VBIMycBov88188_0065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0305.
HOGENOM	HOG000113195.
KO	K02314.
OMA	LPRHAIQ.
ProtClustDB	PRK07773.
Family and domain databases
Gene3D	1.10.860.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. [Graphical view]
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF48024. DNA_helicase_DnaB-like_N. 1 hit.
TIGRFAMs	TIGR00665. DnaB. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS51199. SF4_HELICASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_MYCBO

Accession

Primary (citable) accession number: P59966

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents