Reviewed, UniProtKB/Swiss-Prot P59966 (DNAB_MYCBO)

Names and origin

Protein names

Recommended name:
    Replicative DNA helicase
    EC=3.6.1.-
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease PI-MboHIP
              EC=3.1.-.-
        Alternative name(s):
            Mbo dnaB intein

Gene names

Name:	dnaB
Ordered Locus Names:	Mb0059

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity. The intein is an endonuclease Potential.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication Intron homing
Cellular component	Primosome
Domain	Repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Helicase Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Replicative DNA helicase, 1st part By similarity		PRO_0000013276
	Chain	400 – 815	416	Endonuclease PI-MboHIP		PRO_0000013277
	Chain	816 – 874	59	Replicative DNA helicase, 2nd part By similarity		PRO_0000013278
Regions
	Domain	195 – 462	268	SF4 helicase; first part
	Domain	582 – 660	79	DOD-type homing endonuclease
	Domain	612 – 874	263	SF4 helicase; second part
	Nucleotide binding	226 – 233	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P59966-1 [UniParc]. Last modified October 31, 2003. Version 1. Checksum: 3CFA596E1C7CE7CC	FASTA	874	96,818
	10 20 30 40 50 60 MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE RLRPGDFYRP 70 80 90 100 110 120 AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA PYLHTLISTV PTAANAGYYA 130 140 150 160 170 180 SIVAEKALLR RLVEAGTRVV QYGYAGAEGA DVAEVVDRAQ AEIYDVADRR LSEDFVALED 190 200 210 220 230 240 LLQPTMDEID AIASSGGLAR GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM 250 260 270 280 290 300 RSCSIRHRMA SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 310 320 330 340 350 360 EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR QVEVSEFSRH 370 380 390 400 410 420 LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC LTASTRILRA DTGAEVAFGE 430 440 450 460 470 480 LMRSGERPMV WSLDERLRMV ARPMINVFPS GRKEVFRLRL ASGREVEATG SHPFMKFEGW 490 500 510 520 530 540 TPLAQLKVGD RIAAPRRVPE PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL 550 560 570 580 590 600 AAVTVSAAHS DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 610 620 630 640 650 660 VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD VAQLLLRVGI 670 680 690 700 710 720 FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA VAAQEMLRQL KGPVRNPNLD 730 740 750 760 770 780 SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW KHSPSRSRPH RAEARIEDRA IHELARGDAY 790 800 810 820 830 840 WDTVVEITSI GDQHVFDGTV SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG 850 860 870 GEADFILAKH RNGPTKTVTV AHQLHLSRFA NMAR

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248334 Genomic DNA. Translation: CAD92921.1.
RefSeq	NP_853728.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1094330.
GenomeReviews	Gene locus Mb0059 in contig BX248333_GR.
KEGG	mbo:Mb0059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59966.
OMA	WDEIVSI
Enzyme and pathway databases
BioCyc	MBOV233413:MB0059-MON.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR007692. DNA_helicase_DnaB. IPR007694. DNA_helicase_DnaB-like_C. IPR007693. DNA_helicase_DnaB-like_N. IPR016136. DNA_helicase_N/primase_C. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. [Graphical view]
Gene3D	G3DSA:1.10.860.10. DnaB_N. 1 hit.
Pfam	PF00772. DnaB. 1 hit. PF03796. DnaB_C. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00665. DnaB. 1 hit. TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS51199. SF4_HELICASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_MYCBO

Accession

Primary (citable) accession number: P59966

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	October 31, 2003
Last modified:	November 24, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)