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P59966

- DNAB_MYCBO

UniProt

P59966 - DNAB_MYCBO

Protein

Replicative DNA helicase

Gene

dnaB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (31 Oct 2003)
      Previous versions | rss
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    Functioni

    Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity
    The intein is an endonuclease.Curated

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi226 – 2338ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro
    4. endonuclease activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA replication, Intron homing

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiN10.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicative DNA helicase (EC:3.6.4.12)
    Cleaved into the following chain:
    Alternative name(s):
    Mbo dnaB intein
    Gene namesi
    Name:dnaB
    Ordered Locus Names:Mb0059
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 399399Replicative DNA helicase, 1st partBy similarityPRO_0000013276Add
    BLAST
    Chaini400 – 815416Endonuclease PI-MboHIPPRO_0000013277Add
    BLAST
    Chaini816 – 87459Replicative DNA helicase, 2nd partBy similarityPRO_0000013278Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb0059.

    Structurei

    3D structure databases

    ProteinModelPortaliP59966.
    SMRiP59966. Positions 23-167.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini195 – 462268SF4 helicase; first partPROSITE-ProRule annotationAdd
    BLAST
    Domaini582 – 66079DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST
    Domaini612 – 874263SF4 helicase; second partPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. DnaB subfamily.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
    Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0305.
    HOGENOMiHOG000113195.
    KOiK02314.
    OMAiIAVHNSI.
    OrthoDBiEOG6T4RW5.

    Family and domain databases

    Gene3Di1.10.860.10. 1 hit.
    2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00382. AAA. 1 hit.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48024. SSF48024. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR00665. DnaB. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51199. SF4_HELICASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P59966-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE    50
    RLRPGDFYRP AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA 100
    PYLHTLISTV PTAANAGYYA SIVAEKALLR RLVEAGTRVV QYGYAGAEGA 150
    DVAEVVDRAQ AEIYDVADRR LSEDFVALED LLQPTMDEID AIASSGGLAR 200
    GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM RSCSIRHRMA 250
    SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS 300
    EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR 350
    QVEVSEFSRH LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC 400
    LTASTRILRA DTGAEVAFGE LMRSGERPMV WSLDERLRMV ARPMINVFPS 450
    GRKEVFRLRL ASGREVEATG SHPFMKFEGW TPLAQLKVGD RIAAPRRVPE 500
    PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL AAVTVSAAHS 550
    DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC 600
    VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD 650
    VAQLLLRVGI FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA 700
    VAAQEMLRQL KGPVRNPNLD SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW 750
    KHSPSRSRPH RAEARIEDRA IHELARGDAY WDTVVEITSI GDQHVFDGTV 800
    SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG GEADFILAKH 850
    RNGPTKTVTV AHQLHLSRFA NMAR 874
    Length:874
    Mass (Da):96,818
    Last modified:October 31, 2003 - v1
    Checksum:i3CFA596E1C7CE7CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO41292.1.
    RefSeqiNP_853728.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD92921; CAD92921; Mb0059.
    GeneIDi1094330.
    KEGGimbo:Mb0059.
    PATRICi18001794. VBIMycBov88188_0065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO41292.1 .
    RefSeqi NP_853728.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali P59966.
    SMRi P59966. Positions 23-167.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb0059.

    Protein family/group databases

    MEROPSi N10.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD92921 ; CAD92921 ; Mb0059 .
    GeneIDi 1094330.
    KEGGi mbo:Mb0059.
    PATRICi 18001794. VBIMycBov88188_0065.

    Phylogenomic databases

    eggNOGi COG0305.
    HOGENOMi HOG000113195.
    KOi K02314.
    OMAi IAVHNSI.
    OrthoDBi EOG6T4RW5.

    Family and domain databases

    Gene3Di 1.10.860.10. 1 hit.
    2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR007692. DNA_helicase_DnaB.
    IPR007694. DNA_helicase_DnaB-like_C.
    IPR007693. DNA_helicase_DnaB-like_N.
    IPR016136. DNA_helicase_N/primase_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00772. DnaB. 1 hit.
    PF03796. DnaB_C. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00382. AAA. 1 hit.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48024. SSF48024. 1 hit.
    SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR00665. DnaB. 1 hit.
    TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS51199. SF4_HELICASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiDNAB_MYCBO
    AccessioniPrimary (citable) accession number: P59966
    Secondary accession number(s): X2BDX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: October 31, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3