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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity
The intein is an endonuclease.Curated

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi226 – 233ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Helicase, Hydrolase, Nuclease
Biological processDNA replication, Intron homing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-62-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Alternative name(s):
Mbo dnaB intein
Gene namesi
Name:dnaB
Ordered Locus Names:BQ2027_MB0059
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000132761 – 399Replicative DNA helicase, 1st partBy similarityAdd BLAST399
ChainiPRO_0000013277400 – 815Endonuclease PI-MboHIPAdd BLAST416
ChainiPRO_0000013278816 – 874Replicative DNA helicase, 2nd partBy similarityAdd BLAST59

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliP59966
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini195 – 462SF4 helicase; first partPROSITE-ProRule annotationAdd BLAST268
Domaini582 – 660DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST79
Domaini612 – 874SF4 helicase; second partPROSITE-ProRule annotationAdd BLAST263

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000113195
OMAiRSHEKFV

Family and domain databases

CDDicd00984 DnaB_C, 1 hit
Gene3Di1.10.860.10, 1 hit
3.10.28.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR036185 DNA_heli_DnaB-like_N_sf
IPR007692 DNA_helicase_DnaB
IPR007694 DNA_helicase_DnaB-like_C
IPR007693 DNA_helicase_DnaB-like_N
IPR016136 DNA_helicase_N/primase_C
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00772 DnaB, 1 hit
PF03796 DnaB_C, 1 hit
PF14528 LAGLIDADG_3, 1 hit
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00305 HintC, 1 hit
SM00306 HintN, 1 hit
SUPFAMiSSF48024 SSF48024, 1 hit
SSF51294 SSF51294, 2 hits
SSF52540 SSF52540, 1 hit
SSF55608 SSF55608, 1 hit
TIGRFAMsiTIGR00665 DnaB, 1 hit
TIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50819 INTEIN_ENDONUCLEASE, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS51199 SF4_HELICASE, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59966-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE
60 70 80 90 100
RLRPGDFYRP AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA
110 120 130 140 150
PYLHTLISTV PTAANAGYYA SIVAEKALLR RLVEAGTRVV QYGYAGAEGA
160 170 180 190 200
DVAEVVDRAQ AEIYDVADRR LSEDFVALED LLQPTMDEID AIASSGGLAR
210 220 230 240 250
GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM RSCSIRHRMA
260 270 280 290 300
SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS
310 320 330 340 350
EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR
360 370 380 390 400
QVEVSEFSRH LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC
410 420 430 440 450
LTASTRILRA DTGAEVAFGE LMRSGERPMV WSLDERLRMV ARPMINVFPS
460 470 480 490 500
GRKEVFRLRL ASGREVEATG SHPFMKFEGW TPLAQLKVGD RIAAPRRVPE
510 520 530 540 550
PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL AAVTVSAAHS
560 570 580 590 600
DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC
610 620 630 640 650
VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD
660 670 680 690 700
VAQLLLRVGI FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA
710 720 730 740 750
VAAQEMLRQL KGPVRNPNLD SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW
760 770 780 790 800
KHSPSRSRPH RAEARIEDRA IHELARGDAY WDTVVEITSI GDQHVFDGTV
810 820 830 840 850
SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG GEADFILAKH
860 870
RNGPTKTVTV AHQLHLSRFA NMAR
Length:874
Mass (Da):96,818
Last modified:October 31, 2003 - v1
Checksum:i3CFA596E1C7CE7CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIT98432.1
RefSeqiNP_853728.1, NC_002945.3
WP_003400547.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO41292; CDO41292; Mb0059
PATRICifig|233413.5.peg.65

Similar proteinsi

Entry informationi

Entry nameiDNAB_MYCBO
AccessioniPrimary (citable) accession number: P59966
Secondary accession number(s): A0A1R3XVB4, X2BDX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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