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Protein

Thiazole synthase

Gene

thiG

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S (By similarity).By similarity

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Schiff-base intermediate with DXPBy similarity
Binding sitei159 – 1591DXP; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthase (EC:2.8.1.10)
Gene namesi
Name:thiG
Ordered Locus Names:Mb0425
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Thiazole synthasePRO_0000162829Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP59948.
SMRiP59948. Positions 4-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni185 – 1862DXP bindingBy similarity
Regioni207 – 2082DXP bindingBy similarity

Sequence similaritiesi

Belongs to the ThiG family.Curated

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

P59948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESKLVIGD RSFASRLIMG TGGATNLAVL EQALIASGTE LTTVAIRRVD
60 70 80 90 100
ADGGTGLLDL LNRLGITPLP NTAGCRSAAE AVLTAQLARE ALNTNWVKLE
110 120 130 140 150
VIADERTLWP DAVELVRAAE QLVDDGFVVL PYTTDDPVLA RRLEDTGCAA
160 170 180 190 200
VMPLGSPIGT GLGIANPHNI EMIVAGARVP VVLDAGIGTA SDAALAMELG
210 220 230 240 250
CDAVLLASAV TRAADPPAMA AAMAAAVTAG YLARCAGRIP KRFWAQASSP

AR
Length:252
Mass (Da):25,894
Last modified:October 31, 2003 - v1
Checksum:i91B8B1AE28115512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41664.1.
RefSeqiNP_854088.1. NC_002945.3.
WP_003402126.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO41664; CDO41664; Mb0425.
GeneIDi1091430.
KEGGimbo:Mb0425.
PATRICi18002589. VBIMycBov88188_0463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41664.1.
RefSeqiNP_854088.1. NC_002945.3.
WP_003402126.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP59948.
SMRiP59948. Positions 4-242.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO41664; CDO41664; Mb0425.
GeneIDi1091430.
KEGGimbo:Mb0425.
PATRICi18002589. VBIMycBov88188_0463.

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_MYCBO
AccessioniPrimary (citable) accession number: P59948
Secondary accession number(s): X2BF00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.