Reviewed, UniProtKB/Swiss-Prot P59934 (COOS1_CARHZ)

Names and origin

Protein names

Recommended name:
    Carbon monoxide dehydrogenase 1
      Short name=CODH 1
    EC=1.2.99.2

Gene names

Name:	cooS1
Ordered Locus Names:	CHY_1824

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	CODH oxidizes carbon monoxide coupled, via cooF, to the reduction of a hydrogen cation by a hydrogenase (possibly cooH) By similarity.
Catalytic activity	CO + H2O + A = CO2 + AH2.
Cofactor	Binds 3 4Fe-4S clusters per homodimer By similarity. Binds 2 nickel-iron-sulfur clusters per homodimer By similarity.
Enzyme regulation	Inactivated by O2.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Loosely attached to the membrane, probably via cooF. Ref.2
Sequence similarities	Belongs to the Ni-containing carbon monoxide dehydrogenase family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	generation of precursor metabolites and energy Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 636	635	Carbon monoxide dehydrogenase 1		PRO_0000157137
Sites
	Metal binding	38	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	46	1	Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	47	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	55	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	69	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	262	1	Nickel-iron-sulfur By similarity
	Metal binding	297	1	Nickel-iron-sulfur By similarity
	Metal binding	335	1	Nickel-iron-sulfur By similarity
	Metal binding	448	1	Nickel-iron-sulfur By similarity
	Metal binding	478	1	Nickel-iron-sulfur By similarity
	Metal binding	528	1	Nickel-iron-sulfur By similarity
Experimental info
	Sequence conflict	10	1	P → D AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P59934-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 78CDD333C2A296C7	FASTA	636	67,679
	10 20 30 40 50 60 MSNWKNSVDP AVDYLLPIAK KAGIETAWDR YEAMQPQCGF GELGVCCRIC WKGPCRIDPF 70 80 90 100 110 120 GNGPQRGVCG ADAHTIVARN LIRMIAAGAA AHSEHGRHIA LTLLEVGEGH APAYRIKDEQ 130 140 150 160 170 180 KLRKIAEKLN LAPAGKDIRQ VAKEVALASL DDYSRQKRNV PCNWAKETLT AERVDKLAEL 190 200 210 220 230 240 GVMPHNIDAV ITEIMGRTHV GCDADAVNLL LGGIKGALAD YTGMCLSTEL SDVIFGTPKP 250 260 270 280 290 300 VITQANLGVL KEDAVNIAVH GHNPLLSEII CDVALKMNEE AKKAGAKEGI NVVGICCTGN 310 320 330 340 350 360 EVMMRRGIPL ATNYLSQEMA IITGALDAMV VDVQCIMPAL TSVAECFHTE IITTMAENKI 370 380 390 400 410 420 TGATHIEFRE DSAVESAKKI VEVAIEAFKK RDKRKVNIPD CKQTAITGFS AEAIMAVLSK 430 440 450 460 470 480 LNANDPLKPL IDNIINGNIQ GIALFAGCNN PKAIHDNSFI TIAKELAKNN VLMLATGCGA 490 500 510 520 530 540 GAFAKNGLMT QEATEAYAGE SLKAVLTALG KAAGLNGPLP LVLHMGSCVD NSRAVNVAVA 550 560 570 580 590 600 IANKLGVDLD KLPLVASAPE FMSEKAVAIG TWAVTLGIPT HIGIVPQIMG SSVVVEFLTE 610 620 630 KAKDLLGGYF IVETNPELAA AKLVAVIKER RRGLGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Two membrane-associated NiFeS-carbon monoxide dehydrogenases from the anaerobic carbon-monoxide-utilizing eubacterium Carboxydothermus hydrogenoformans." Svetlitchnyi V., Peschel C., Acker G., Meyer O. J. Bacteriol. 183:5134-5144(2001) [PubMed: 11489867] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-23, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000141 Genomic DNA. Translation: ABB14432.1.
RefSeq	YP_360644.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P59934.
Genome annotation databases
GeneID	3728783.
GenomeReviews	Gene locus CHY_1824 in contig CP000141_GR.
KEGG	chy:CHY_1824.
NMPDR	fig|246194.3.peg.2363.
TIGR	CHY_1824.
Phylogenomic databases
HOGENOM	P59934.
OMA	KGPCRID.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_1824-MON.
Family and domain databases
InterPro	IPR016101. CO_DH_a-bundle. IPR010047. CO_DH_cat. IPR004137. Prismane. IPR016099. Prismane-like_a/b-sand. [Graphical view]
Gene3D	G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 1 hit.
Pfam	PF03063. Prismane. 1 hit. [Graphical view]
PIRSF	PIRSF005023. CODH. 1 hit.
TIGRFAMs	TIGR01702. CO_DH_cata. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COOS1_CARHZ

Accession

Primary (citable) accession number: P59934
Secondary accession number(s): Q3AB40

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 41 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)