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P59895

- NEK6_RAT

UniProt

P59895 - NEK6_RAT

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Protein
Serine/threonine-protein kinase Nek6
Gene
Nek6
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence By similarity. Phosphorylates RPS6KB1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741ATP By similarity
Sitei108 – 1081Autoinhibitory By similarity
Active sitei172 – 1721Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 599ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein kinase binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. signal transducer activity Source: Ensembl

GO - Biological processi

  1. G2 DNA damage checkpoint Source: UniProtKB
  2. apoptotic process Source: UniProtKB-KW
  3. chromosome segregation Source: UniProtKB
  4. mitotic nuclear division Source: UniProtKB-KW
  5. peptidyl-serine phosphorylation Source: Ensembl
  6. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
  7. protein autophosphorylation Source: UniProtKB
  8. protein phosphorylation Source: UniProtKB
  9. regulation of mitotic metaphase/anaphase transition Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198320. Activation of NIMA Kinases NEK9, NEK6, NEK7.
REACT_198363. Nuclear Pore Complex (NPC) Disassembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek6 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 6
Short name:
NimA-related protein kinase 6
Gene namesi
Name:Nek6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi727779. Nek6.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Nucleus speckle By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole By similarity
Note: Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. nuclear speck Source: UniProtKB-SubCell
  4. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Serine/threonine-protein kinase Nek6
PRO_0000086429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371Phosphoserine By similarity
Modified residuei202 – 2021Phosphothreonine By similarity
Modified residuei206 – 2061Phosphoserine; by NEK9 By similarity
Modified residuei210 – 2101Phosphothreonine By similarity

Post-translational modificationi

Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210 By similarity.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Highly expressed in the liver.1 Publication

Gene expression databases

GenevestigatoriP59895.

Interactioni

Subunit structurei

Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7, STAT3 and TRIP4 By similarity. Interacts with RPS6KB1.

Structurei

3D structure databases

ProteinModelPortaliP59895.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 310266Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Interaction with ARHGAP33, ANKRA2, CDC42, PRDX3, RAD26L, RBBP6, RPS7 and TRIP4 By similarity
Add
BLAST
Regioni267 – 2704Interaction with APBB1 By similarity

Domaini

Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00720000108402.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiP59895.
KOiK08857.
OMAiTLADFQI.
OrthoDBiEOG74J983.
PhylomeDBiP59895.
TreeFamiTF105135.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59895-1 [UniParc]FASTAAdd to Basket

« Hide

MAGQPSHMPH GGSPNHLCHV LGPAHPPDPQ RHPNTLSFRC SLADFQIEKK    50
IGRGQFSEVY KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL 100
NHPNIIKYLD SFIEDNELNI VLELADAGDL SQMIKYFKKQ KRLIPERTVW 150
KYFVQLCSAV EHMHSRRVMH RDIKPANVFI TATGIVKLGD LGLGRFFSSE 200
TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM AALQSPFYGD 250
KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCIYPDP NHRPDIEYVH 300
QVAKQMHVWT SST 313
Length:313
Mass (Da):35,835
Last modified:October 3, 2003 - v1
Checksum:i4CC398D70916021F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY334013 mRNA. Translation: AAP97428.1.
RefSeqiNP_001264161.1. NM_001277232.1.
NP_891998.1. NM_182953.2.
XP_006234162.1. XM_006234100.1.
UniGeneiRn.18744.

Genome annotation databases

EnsembliENSRNOT00000014630; ENSRNOP00000014631; ENSRNOG00000010897.
GeneIDi360161.
KEGGirno:360161.
UCSCiRGD:727779. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY334013 mRNA. Translation: AAP97428.1 .
RefSeqi NP_001264161.1. NM_001277232.1.
NP_891998.1. NM_182953.2.
XP_006234162.1. XM_006234100.1.
UniGenei Rn.18744.

3D structure databases

ProteinModelPortali P59895.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000014630 ; ENSRNOP00000014631 ; ENSRNOG00000010897 .
GeneIDi 360161.
KEGGi rno:360161.
UCSCi RGD:727779. rat.

Organism-specific databases

CTDi 10783.
RGDi 727779. Nek6.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00720000108402.
HOGENOMi HOG000233029.
HOVERGENi HBG105886.
InParanoidi P59895.
KOi K08857.
OMAi TLADFQI.
OrthoDBi EOG74J983.
PhylomeDBi P59895.
TreeFami TF105135.

Enzyme and pathway databases

Reactomei REACT_198320. Activation of NIMA Kinases NEK9, NEK6, NEK7.
REACT_198363. Nuclear Pore Complex (NPC) Disassembly.

Miscellaneous databases

NextBioi 672720.
PROi P59895.

Gene expression databases

Genevestigatori P59895.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR00109. TYRKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of Rattus norvegicus NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6)."
    Zhou G., Zhang Y., Ni J.
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase."
    Belham C., Comb M.J., Avruch J.
    Curr. Biol. 11:1155-1167(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNEK6_RAT
AccessioniPrimary (citable) accession number: P59895
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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