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P59895

- NEK6_RAT

UniProt

P59895 - NEK6_RAT

Protein

Serine/threonine-protein kinase Nek6

Gene

Nek6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (03 Oct 2003)
      Previous versions | rss
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    Functioni

    Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence By similarity. Phosphorylates RPS6KB1.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei74 – 741ATPPROSITE-ProRule annotation
    Sitei108 – 1081AutoinhibitoryBy similarity
    Active sitei172 – 1721Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi51 – 599ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein kinase binding Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB
    5. signal transducer activity Source: Ensembl

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. chromosome segregation Source: UniProtKB
    3. G2 DNA damage checkpoint Source: UniProtKB
    4. mitotic nuclear division Source: UniProtKB-KW
    5. peptidyl-serine phosphorylation Source: Ensembl
    6. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
    7. protein autophosphorylation Source: UniProtKB
    8. protein phosphorylation Source: UniProtKB
    9. regulation of mitotic metaphase/anaphase transition Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198320. Activation of NIMA Kinases NEK9, NEK6, NEK7.
    REACT_198363. Nuclear Pore Complex (NPC) Disassembly.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase Nek6 (EC:2.7.11.1)
    Alternative name(s):
    Never in mitosis A-related kinase 6
    Short name:
    NimA-related protein kinase 6
    Gene namesi
    Name:Nek6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi727779. Nek6.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Nucleus speckle By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. nuclear speck Source: UniProtKB-SubCell
    4. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Serine/threonine-protein kinase Nek6PRO_0000086429Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei37 – 371PhosphoserineBy similarity
    Modified residuei202 – 2021PhosphothreonineBy similarity
    Modified residuei206 – 2061Phosphoserine; by NEK9By similarity
    Modified residuei210 – 2101PhosphothreonineBy similarity

    Post-translational modificationi

    Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210 By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Highly expressed in the liver.1 Publication

    Gene expression databases

    GenevestigatoriP59895.

    Interactioni

    Subunit structurei

    Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7, STAT3 and TRIP4 By similarity. Interacts with RPS6KB1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP59895.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 310266Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4444Interaction with ARHGAP33, ANKRA2, CDC42, PRDX3, RAD26L, RBBP6, RPS7 and TRIP4By similarityAdd
    BLAST
    Regioni267 – 2704Interaction with APBB1By similarity

    Domaini

    Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 By similarity.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108402.
    HOGENOMiHOG000233029.
    HOVERGENiHBG105886.
    InParanoidiP59895.
    KOiK08857.
    OMAiTLADFQI.
    OrthoDBiEOG74J983.
    PhylomeDBiP59895.
    TreeFamiTF105135.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR00109. TYRKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59895-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGQPSHMPH GGSPNHLCHV LGPAHPPDPQ RHPNTLSFRC SLADFQIEKK    50
    IGRGQFSEVY KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL 100
    NHPNIIKYLD SFIEDNELNI VLELADAGDL SQMIKYFKKQ KRLIPERTVW 150
    KYFVQLCSAV EHMHSRRVMH RDIKPANVFI TATGIVKLGD LGLGRFFSSE 200
    TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM AALQSPFYGD 250
    KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCIYPDP NHRPDIEYVH 300
    QVAKQMHVWT SST 313
    Length:313
    Mass (Da):35,835
    Last modified:October 3, 2003 - v1
    Checksum:i4CC398D70916021F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY334013 mRNA. Translation: AAP97428.1.
    RefSeqiNP_001264161.1. NM_001277232.1.
    NP_891998.1. NM_182953.2.
    XP_006234162.1. XM_006234100.1.
    UniGeneiRn.18744.

    Genome annotation databases

    EnsembliENSRNOT00000014630; ENSRNOP00000014631; ENSRNOG00000010897.
    GeneIDi360161.
    KEGGirno:360161.
    UCSCiRGD:727779. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY334013 mRNA. Translation: AAP97428.1 .
    RefSeqi NP_001264161.1. NM_001277232.1.
    NP_891998.1. NM_182953.2.
    XP_006234162.1. XM_006234100.1.
    UniGenei Rn.18744.

    3D structure databases

    ProteinModelPortali P59895.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000014630 ; ENSRNOP00000014631 ; ENSRNOG00000010897 .
    GeneIDi 360161.
    KEGGi rno:360161.
    UCSCi RGD:727779. rat.

    Organism-specific databases

    CTDi 10783.
    RGDi 727779. Nek6.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108402.
    HOGENOMi HOG000233029.
    HOVERGENi HBG105886.
    InParanoidi P59895.
    KOi K08857.
    OMAi TLADFQI.
    OrthoDBi EOG74J983.
    PhylomeDBi P59895.
    TreeFami TF105135.

    Enzyme and pathway databases

    Reactomei REACT_198320. Activation of NIMA Kinases NEK9, NEK6, NEK7.
    REACT_198363. Nuclear Pore Complex (NPC) Disassembly.

    Miscellaneous databases

    NextBioi 672720.
    PROi P59895.

    Gene expression databases

    Genevestigatori P59895.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00109. TYRKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of Rattus norvegicus NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6)."
      Zhou G., Zhang Y., Ni J.
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase."
      Belham C., Comb M.J., Avruch J.
      Curr. Biol. 11:1155-1167(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiNEK6_RAT
    AccessioniPrimary (citable) accession number: P59895
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: October 3, 2003
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3