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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei311Coenzyme AUniRule annotation1
Binding sitei508ATPUniRule annotation1
Binding sitei523ATPUniRule annotation1
Binding sitei531Coenzyme A; via carbonyl oxygenUniRule annotation1
Binding sitei534ATPUniRule annotation1
Metal bindingi545Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi547Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi550Magnesium; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi387 – 389ATPUniRule annotation3
Nucleotide bindingi411 – 416ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-3875-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Synonyms:acs
Ordered Locus Names:BQ2027_MB3691
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002083681 – 651Acetyl-coenzyme A synthetaseAdd BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei617N6-acetyllysineUniRule annotation1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Structurei

3D structure databases

ProteinModelPortaliP59871
SMRiP59871
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 193Coenzyme A bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229981
OMAiDHWWHDL

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P59871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESTPEVSS SYPPPAHFAE HANARAELYR EAEEDRLAFW AKQANRLSWT
60 70 80 90 100
TPFTEVLDWS EAPFAKWFVG GELNVAYNCV DRHVEAGHGD RVAIHWEGEP
110 120 130 140 150
VGDRRTLTYS DLLAEVSKAA NALTDLGLVA GDRVAIYLPL IPEAVIAMLA
160 170 180 190 200
CARLGIMHSV VFGGFTAAAL QARIVDAQAK LLITADGQFR RGKPSPLKAA
210 220 230 240 250
ADEALAAIPD CSVEHVLVVR RTGIEMAWSE GRDLWWHHVV GSASPAHTPE
260 270 280 290 300
PFDSEHPLFL LYTSGTTGKP KGIMHTSGGY LTQCCYTMRT IFDVKPDSDV
310 320 330 340 350
FWCTADIGWV TGHTYGVYGP LCNGVTEVLY EGTPDTPDRH RHFQIIEKYG
360 370 380 390 400
VTIYYTAPTL IRMFMKWGRE IPDSHDLSSL RLLGSVGEPI NPEAWRWYRD
410 420 430 440 450
VIGGGRTPLV DTWWQTETGS AMISPLPGIA AAKPGSAMTP LPGISAKIVD
460 470 480 490 500
DHGDPLPPHT EGAQHVTGYL VLDQPWPSML RGIWGDPARY WHSYWSKFSD
510 520 530 540 550
KGYYFAGDGA RIDPDGAIWV LGRIDDVMNV SGHRISTAEV ESALVAHSGV
560 570 580 590 600
AEAAVVGVTD ETTTQAICAF VVLRANYAPH DRTAEELRTE VARVISPIAR
610 620 630 640 650
PRDVHVVPEL PKTRSGKIMR RLLRDVAENR ELGDTSTLLD PTVFDAIRAA

K
Length:651
Mass (Da):71,548
Last modified:September 26, 2003 - v1
Checksum:i5325A7A21BB2A493
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU02319.1
RefSeqiNP_857330.1, NC_002945.3
WP_010950924.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO44962; CDO44962; Mb3691
PATRICifig|233413.5.peg.4042

Similar proteinsi

Entry informationi

Entry nameiACSA_MYCBO
AccessioniPrimary (citable) accession number: P59871
Secondary accession number(s): A0A1R3Y4W5, X2BNU6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: April 25, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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