P59870 (KAX55_MESTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 5.5 Alternative name(s): Tamapin-2
Organism	Mesobuthus tamulus (Eastern Indian scorpion) (Buthus tamulus)
Taxonomic identifier	34647 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus

Protein attributes

Sequence length	31 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Blocks small conductance calcium-activated potassium channels. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 5 subfamily.
Mass spectrometry	Molecular mass is 3431.4±0.2 Da from positions 1 - 31. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 31	31	Potassium channel toxin alpha-KTx 5.5		PRO_0000044931
Amino acid modifications
	Modified residue	31	1	Histidine amide Probable
	Disulfide bond	3 ↔ 21		By similarity
	Disulfide bond	8 ↔ 26		By similarity
	Disulfide bond	12 ↔ 28		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59870 [UniParc]. Last modified September 26, 2003. Version 1. Checksum: E4D43CDC54CA415C	FASTA	31	3,439
	10 20 30 AFCNLRRCEL SCRSLGLLGK CIGEECKCVP H

References

[1]

"Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus) that targets small conductance Ca2+-activated K+ channels and afterhyperpolarization currents in central neurons." Pedarzani P., D'hoedt D., Doorty K.B., Wadsworth J.D.F., Joseph J.S., Jeyaseelan K., Kini R.M., Gadre S.V., Sapatnekar S.M., Stocker M., Strong P.N. J. Biol. Chem. 277:46101-46109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY, FUNCTION. Tissue: Venom.

Cross-references

3D structure databases
ProteinModelPortal	P59870.
SMR	P59870. Positions 2-31.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX55_MESTA

Accession

Primary (citable) accession number: P59870

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	September 26, 2003
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program