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P59847

- KAX25_CENLM

UniProt

P59847 - KAX25_CENLM

Protein

Potassium channel toxin alpha-KTx 2.5

Gene
N/A
Organism
Centruroides limbatus (Bark scorpion)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Potent selective inhibitor of Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3 voltage-gated potassium channels. Weak inhibitor of Kv1.6/KCNA6 potassium channel.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei28 – 281Basic residue of the functional dyadBy similarity
    Sitei37 – 371Aromatic residue of the functional dyadBy similarity

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel toxin alpha-KTx 2.5
    Alternative name(s):
    Hongotoxin-1
    Short name:
    HgTX1
    OrganismiCentruroides limbatus (Bark scorpion)
    Taxonomic identifieri244936 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeCentruroides

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi19 – 191A → C: No loss of activity. 2 Publications
    Mutagenesisi19 – 191A → Y: No loss of activity; when associated with F-37. 2 Publications
    Mutagenesisi37 – 371Y → F: No loss of activity; when associated with Y-19. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 3939Potassium channel toxin alpha-KTx 2.5PRO_0000044903Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi7 ↔ 291 Publication
    Disulfide bondi13 ↔ 341 Publication
    Disulfide bondi17 ↔ 361 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    Secondary structure

    1
    39
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 103
    Helixi11 – 133
    Helixi14 – 207
    Beta strandi26 – 305
    Beta strandi33 – 375

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HLYNMR-A1-39[»]
    ProteinModelPortaliP59847.
    SMRiP59847. Positions 1-39.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP59847.

    Family & Domainsi

    Domaini

    Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view]
    PfamiPF00451. Toxin_2. 1 hit.
    [Graphical view]
    PRINTSiPR00286. CHARYBDTOXIN.
    ProDomiPD003586. Scorpion_toxinS. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF57095. SSF57095. 1 hit.
    PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59847-1 [UniParc]FASTAAdd to Basket

    « Hide

    TVIDVKCTSP KQCLPPCKAQ FGIRAGAKCM NGKCKCYPH               39
    Length:39
    Mass (Da):4,226
    Last modified:September 19, 2003 - v1
    Checksum:iB4ABC83F8EE7E637
    GO

    Mass spectrometryi

    Molecular mass is 4219 Da from positions 1 - 39. Determined by ESI. 1 Publication

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HLY NMR - A 1-39 [» ]
    ProteinModelPortali P59847.
    SMRi P59847. Positions 1-39.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P59847.

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR001947. Scorpion_toxinS_K_inh.
    [Graphical view ]
    Pfami PF00451. Toxin_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00286. CHARYBDTOXIN.
    ProDomi PD003586. Scorpion_toxinS. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    PROSITEi PS01138. SCORP_SHORT_TOXIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Subunit composition of brain voltage-gated potassium channels determined by hongotoxin-1, a novel peptide derived from Centruroides limbatus venom."
      Koschak A., Bugianesi R.M., Mitterdorfer J., Kaczorowski G.J., Garcia M.L., Knaus H.-G.
      J. Biol. Chem. 273:2639-2644(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, MUTAGENESIS.
      Tissue: Venom.
    2. "Synthesis, characterization, and application of cy-dye- and alexa-dye-labeled hongotoxin(1) analogues. The first high affinity fluorescence probes for voltage-gated K+ channels."
      Pragl B., Koschak A., Trieb M., Obermair G., Kaufmann W.A., Gerster U., Blanc E., Hahn C., Prinz H., Schuetz G., Darbon H., Gruber H.J., Knaus H.-G.
      Bioconj. Chem. 13:416-425(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, MASS SPECTROMETRY, MUTAGENESIS OF ALA-19 AND TYR-37.
      Tissue: Venom.

    Entry informationi

    Entry nameiKAX25_CENLM
    AccessioniPrimary (citable) accession number: P59847
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: September 19, 2003
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Does not block Kv1.4/KCNA4 and Kv1.5/KCNA5 currents.1 Publication

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Scorpion potassium channel toxins
      Nomenclature of scorpion potassium channel toxins and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3