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P59847 (KAX25_CENLM) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin alpha-KTx 2.5
Alternative name(s):
Hongotoxin-1
Short name=HgTX1
OrganismCentruroides limbatus (Bark scorpion)
Taxonomic identifier244936 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeCentruroides

Protein attributes

Sequence length39 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potent selective inhibitor of Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3 voltage-gated potassium channels. Weak inhibitor of Kv1.6/KCNA6 potassium channel. Ref.1

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Domain

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Miscellaneous

Does not block Kv1.4/KCNA4 and Kv1.5/KCNA5 currents (Ref.1).

Sequence similarities

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 2 subfamily.

Mass spectrometry

Molecular mass is 4219 Da from positions 1 - 39. Determined by ESI. Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 3939Potassium channel toxin alpha-KTx 2.5
PRO_0000044903

Sites

Site281Basic residue of the functional dyad By similarity
Site371Aromatic residue of the functional dyad By similarity

Amino acid modifications

Disulfide bond7 ↔ 29 Ref.2
Disulfide bond13 ↔ 34 Ref.2
Disulfide bond17 ↔ 36 Ref.2

Experimental info

Mutagenesis191A → C: No loss of activity. Ref.2
Mutagenesis191A → Y: No loss of activity; when associated with F-37. Ref.2
Mutagenesis371Y → F: No loss of activity; when associated with Y-19. Ref.2

Secondary structure

......... 39
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P59847 [UniParc].

Last modified September 19, 2003. Version 1.
Checksum: B4ABC83F8EE7E637

FASTA394,226
        10         20         30 
TVIDVKCTSP KQCLPPCKAQ FGIRAGAKCM NGKCKCYPH 

« Hide

References

[1]"Subunit composition of brain voltage-gated potassium channels determined by hongotoxin-1, a novel peptide derived from Centruroides limbatus venom."
Koschak A., Bugianesi R.M., Mitterdorfer J., Kaczorowski G.J., Garcia M.L., Knaus H.-G.
J. Biol. Chem. 273:2639-2644(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, MUTAGENESIS.
Tissue: Venom.
[2]"Synthesis, characterization, and application of cy-dye- and alexa-dye-labeled hongotoxin(1) analogues. The first high affinity fluorescence probes for voltage-gated K+ channels."
Pragl B., Koschak A., Trieb M., Obermair G., Kaufmann W.A., Gerster U., Blanc E., Hahn C., Prinz H., Schuetz G., Darbon H., Gruber H.J., Knaus H.-G.
Bioconj. Chem. 13:416-425(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, MASS SPECTROMETRY, MUTAGENESIS OF ALA-19 AND TYR-37.
Tissue: Venom.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HLYNMR-A1-39[»]
ProteinModelPortalP59847.
SMRP59847. Positions 1-39.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSPR00286. CHARYBDTOXIN.
ProDomPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF57095. SSF57095. 1 hit.
PROSITEPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP59847.

Entry information

Entry nameKAX25_CENLM
AccessionPrimary (citable) accession number: P59847
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: October 16, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references