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Protein

Potassium channel toxin alpha-KTx 2.5

Gene
N/A
Organism
Centruroides limbatus (Bark scorpion)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potent selective inhibitor of Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3 voltage-gated potassium channels. Weak inhibitor of Kv1.6/KCNA6 potassium channel.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei28 – 281Basic residue of the functional dyadBy similarity
Sitei37 – 371Aromatic residue of the functional dyadBy similarity

GO - Molecular functioni

  1. ion channel inhibitor activity Source: InterPro

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel toxin alpha-KTx 2.5
Alternative name(s):
Hongotoxin-1
Short name:
HgTX1
OrganismiCentruroides limbatus (Bark scorpion)
Taxonomic identifieri244936 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeCentruroides

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191A → C: No loss of activity. 1 Publication
Mutagenesisi19 – 191A → Y: No loss of activity; when associated with F-37. 1 Publication
Mutagenesisi37 – 371Y → F: No loss of activity; when associated with Y-19. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3939Potassium channel toxin alpha-KTx 2.5PRO_0000044903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 291 Publication
Disulfide bondi13 ↔ 341 Publication
Disulfide bondi17 ↔ 361 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
39
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 103Combined sources
Helixi11 – 133Combined sources
Helixi14 – 207Combined sources
Beta strandi26 – 305Combined sources
Beta strandi33 – 375Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HLYNMR-A1-39[»]
SMRiP59847. Positions 1-39.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59847.

Family & Domainsi

Domaini

Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
TVIDVKCTSP KQCLPPCKAQ FGIRAGAKCM NGKCKCYPH
Length:39
Mass (Da):4,226
Last modified:September 19, 2003 - v1
Checksum:iB4ABC83F8EE7E637
GO

Mass spectrometryi

Molecular mass is 4219 Da from positions 1 - 39. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HLYNMR-A1-39[»]
SMRiP59847. Positions 1-39.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP59847.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]
PfamiPF00451. Toxin_2. 1 hit.
[Graphical view]
PRINTSiPR00286. CHARYBDTOXIN.
ProDomiPD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Subunit composition of brain voltage-gated potassium channels determined by hongotoxin-1, a novel peptide derived from Centruroides limbatus venom."
    Koschak A., Bugianesi R.M., Mitterdorfer J., Kaczorowski G.J., Garcia M.L., Knaus H.-G.
    J. Biol. Chem. 273:2639-2644(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, MUTAGENESIS.
    Tissue: Venom.
  2. "Synthesis, characterization, and application of cy-dye- and alexa-dye-labeled hongotoxin(1) analogues. The first high affinity fluorescence probes for voltage-gated K+ channels."
    Pragl B., Koschak A., Trieb M., Obermair G., Kaufmann W.A., Gerster U., Blanc E., Hahn C., Prinz H., Schuetz G., Darbon H., Gruber H.J., Knaus H.-G.
    Bioconj. Chem. 13:416-425(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS, MASS SPECTROMETRY, MUTAGENESIS OF ALA-19 AND TYR-37.
    Tissue: Venom.

Entry informationi

Entry nameiKAX25_CENLM
AccessioniPrimary (citable) accession number: P59847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: April 1, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not block Kv1.4/KCNA4 and Kv1.5/KCNA5 currents.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.