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Protein

Argininosuccinate synthase

Gene

argG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATP; via amide nitrogen and carbonyl oxygenUniRule annotation2 Publications1
Binding sitei84Citrulline1
Binding sitei89Citrulline1
Binding sitei114ATP; via amide nitrogenUniRule annotation2 Publications1
Binding sitei116Aspartate1
Binding sitei120Aspartate1
Binding sitei120Citrulline1
Binding sitei121Aspartate1
Binding sitei124Citrulline1
Binding sitei173Citrulline1
Binding sitei182Citrulline1
Binding sitei258Citrulline1
Binding sitei270Citrulline1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 14ATPUniRule annotation2 Publications9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.5. 2305.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:TTHA0284
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001486581 – 400Argininosuccinate synthaseAdd BLAST400

Interactioni

Subunit structurei

Homotetramer.UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi300852.TTHA0284.

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi11 – 24Combined sources14
Beta strandi27 – 36Combined sources10
Helixi41 – 51Combined sources11
Beta strandi54 – 60Combined sources7
Helixi62 – 68Combined sources7
Helixi70 – 75Combined sources6
Turni81 – 83Combined sources3
Turni87 – 90Combined sources4
Helixi91 – 106Combined sources16
Beta strandi109 – 112Combined sources4
Beta strandi117 – 120Combined sources4
Helixi121 – 132Combined sources12
Beta strandi137 – 139Combined sources3
Helixi141 – 143Combined sources3
Helixi150 – 159Combined sources10
Beta strandi173 – 176Combined sources4
Beta strandi181 – 185Combined sources5
Helixi186 – 189Combined sources4
Beta strandi201 – 203Combined sources3
Helixi206 – 208Combined sources3
Beta strandi214 – 221Combined sources8
Beta strandi224 – 228Combined sources5
Helixi235 – 248Combined sources14
Beta strandi253 – 259Combined sources7
Beta strandi265 – 271Combined sources7
Helixi273 – 289Combined sources17
Helixi292 – 311Combined sources20
Beta strandi314 – 316Combined sources3
Helixi317 – 330Combined sources14
Beta strandi335 – 342Combined sources8
Beta strandi345 – 352Combined sources8
Helixi360 – 362Combined sources3
Helixi373 – 394Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J1ZX-ray2.10A/B/C/D1-400[»]
1J20X-ray2.00A/B/C/D1-400[»]
1J21X-ray2.20A/B/C/D1-400[»]
1KH1X-ray2.30A/B/C/D1-400[»]
1KH2X-ray2.30A/B/C/D1-400[»]
1KH3X-ray2.15A/B/C/D1-400[»]
1KORX-ray1.95A/B/C/D1-400[»]
ProteinModelPortaliP59846.
SMRiP59846.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59846.

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
PhylomeDBiP59846.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIVLAYSGG LDTSIILKWL KETYRAEVIA FTADIGQGEE VEEAREKALR
60 70 80 90 100
TGASKAIALD LKEEFVRDFV FPMMRAGAVY EGYYLLGTSI ARPLIAKHLV
110 120 130 140 150
RIAEEEGAEA IAHGATGKGN DQVRFELTAY ALKPDIKVIA PWREWSFQGR
160 170 180 190 200
KEMIAYAEAH GIPVPVTQEK PYSMDANLLH ISYEGGVLED PWAEPPKGMF
210 220 230 240 250
RMTQDPEEAP DAPEYVEVEF FEGDPVAVNG ERLSPAALLQ RLNEIGGRHG
260 270 280 290 300
VGRVDIVENR FVGMKSRGVY ETPGGTILYH ARRAVESLTL DREVLHQRDM
310 320 330 340 350
LSPKYAELVY YGFWYAPERE ALQAYFDHVA RSVTGVARLK LYKGNVYVVG
360 370 380 390 400
RKAPKSLYRQ DLVSFDEAGG YDQKDAEGFI KIQALRLRVR ALVEREGHGA
Length:400
Mass (Da):44,816
Last modified:September 19, 2003 - v1
Checksum:iA4DAC90A7C5185C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70107.1.
RefSeqiWP_011227831.1. NC_006461.1.
YP_143550.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70107; BAD70107; BAD70107.
GeneIDi3168176.
KEGGittj:TTHA0284.
PATRICi23955523. VBITheThe93045_0284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70107.1.
RefSeqiWP_011227831.1. NC_006461.1.
YP_143550.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J1ZX-ray2.10A/B/C/D1-400[»]
1J20X-ray2.00A/B/C/D1-400[»]
1J21X-ray2.20A/B/C/D1-400[»]
1KH1X-ray2.30A/B/C/D1-400[»]
1KH2X-ray2.30A/B/C/D1-400[»]
1KH3X-ray2.15A/B/C/D1-400[»]
1KORX-ray1.95A/B/C/D1-400[»]
ProteinModelPortaliP59846.
SMRiP59846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70107; BAD70107; BAD70107.
GeneIDi3168176.
KEGGittj:TTHA0284.
PATRICi23955523. VBITheThe93045_0284.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.
PhylomeDBiP59846.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BRENDAi6.3.4.5. 2305.

Miscellaneous databases

EvolutionaryTraceiP59846.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_THET8
AccessioniPrimary (citable) accession number: P59846
Secondary accession number(s): Q5SLK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.