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P59846

- ASSY_THET8

UniProt

P59846 - ASSY_THET8

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Protein

Argininosuccinate synthase

Gene

argG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP; via amide nitrogen and carbonyl oxygen2 PublicationsUniRule annotation
Binding sitei84 – 841Citrulline
Binding sitei89 – 891Citrulline
Binding sitei114 – 1141ATP; via amide nitrogen2 PublicationsUniRule annotation
Binding sitei116 – 1161Aspartate
Binding sitei120 – 1201Aspartate
Binding sitei120 – 1201Citrulline
Binding sitei121 – 1211Aspartate
Binding sitei124 – 1241Citrulline
Binding sitei173 – 1731Citrulline
Binding sitei182 – 1821Citrulline
Binding sitei258 – 2581Citrulline
Binding sitei270 – 2701Citrulline

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 149ATP2 PublicationsUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-298-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:TTHA0284
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Argininosuccinate synthasePRO_0000148658Add
BLAST

Interactioni

Subunit structurei

Homotetramer.2 PublicationsUniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA0284.

Structurei

Secondary structure

1
400
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi11 – 2414Combined sources
Beta strandi27 – 3610Combined sources
Helixi41 – 5111Combined sources
Beta strandi54 – 607Combined sources
Helixi62 – 687Combined sources
Helixi70 – 756Combined sources
Turni81 – 833Combined sources
Turni87 – 904Combined sources
Helixi91 – 10616Combined sources
Beta strandi109 – 1124Combined sources
Beta strandi117 – 1204Combined sources
Helixi121 – 13212Combined sources
Beta strandi137 – 1393Combined sources
Helixi141 – 1433Combined sources
Helixi150 – 15910Combined sources
Beta strandi173 – 1764Combined sources
Beta strandi181 – 1855Combined sources
Helixi186 – 1894Combined sources
Beta strandi201 – 2033Combined sources
Helixi206 – 2083Combined sources
Beta strandi214 – 2218Combined sources
Beta strandi224 – 2285Combined sources
Helixi235 – 24814Combined sources
Beta strandi253 – 2597Combined sources
Beta strandi265 – 2717Combined sources
Helixi273 – 28917Combined sources
Helixi292 – 31120Combined sources
Beta strandi314 – 3163Combined sources
Helixi317 – 33014Combined sources
Beta strandi335 – 3428Combined sources
Beta strandi345 – 3528Combined sources
Helixi360 – 3623Combined sources
Helixi373 – 39422Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J1ZX-ray2.10A/B/C/D1-400[»]
1J20X-ray2.00A/B/C/D1-400[»]
1J21X-ray2.20A/B/C/D1-400[»]
1KH1X-ray2.30A/B/C/D1-400[»]
1KH2X-ray2.30A/B/C/D1-400[»]
1KH3X-ray2.15A/B/C/D1-400[»]
1KORX-ray1.95A/B/C/D1-400[»]
ProteinModelPortaliP59846.
SMRiP59846. Positions 1-395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59846.

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiAPPEEAY.
OrthoDBiEOG6K9QCV.
PhylomeDBiP59846.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59846-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIVLAYSGG LDTSIILKWL KETYRAEVIA FTADIGQGEE VEEAREKALR
60 70 80 90 100
TGASKAIALD LKEEFVRDFV FPMMRAGAVY EGYYLLGTSI ARPLIAKHLV
110 120 130 140 150
RIAEEEGAEA IAHGATGKGN DQVRFELTAY ALKPDIKVIA PWREWSFQGR
160 170 180 190 200
KEMIAYAEAH GIPVPVTQEK PYSMDANLLH ISYEGGVLED PWAEPPKGMF
210 220 230 240 250
RMTQDPEEAP DAPEYVEVEF FEGDPVAVNG ERLSPAALLQ RLNEIGGRHG
260 270 280 290 300
VGRVDIVENR FVGMKSRGVY ETPGGTILYH ARRAVESLTL DREVLHQRDM
310 320 330 340 350
LSPKYAELVY YGFWYAPERE ALQAYFDHVA RSVTGVARLK LYKGNVYVVG
360 370 380 390 400
RKAPKSLYRQ DLVSFDEAGG YDQKDAEGFI KIQALRLRVR ALVEREGHGA
Length:400
Mass (Da):44,816
Last modified:September 19, 2003 - v1
Checksum:iA4DAC90A7C5185C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008226 Genomic DNA. Translation: BAD70107.1.
RefSeqiYP_143550.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70107; BAD70107; BAD70107.
GeneIDi3168176.
KEGGittj:TTHA0284.
PATRICi23955523. VBITheThe93045_0284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008226 Genomic DNA. Translation: BAD70107.1 .
RefSeqi YP_143550.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J1Z X-ray 2.10 A/B/C/D 1-400 [» ]
1J20 X-ray 2.00 A/B/C/D 1-400 [» ]
1J21 X-ray 2.20 A/B/C/D 1-400 [» ]
1KH1 X-ray 2.30 A/B/C/D 1-400 [» ]
1KH2 X-ray 2.30 A/B/C/D 1-400 [» ]
1KH3 X-ray 2.15 A/B/C/D 1-400 [» ]
1KOR X-ray 1.95 A/B/C/D 1-400 [» ]
ProteinModelPortali P59846.
SMRi P59846. Positions 1-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300852.TTHA0284.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD70107 ; BAD70107 ; BAD70107 .
GeneIDi 3168176.
KEGGi ttj:TTHA0284.
PATRICi 23955523. VBITheThe93045_0284.

Phylogenomic databases

eggNOGi COG0137.
HOGENOMi HOG000230093.
KOi K01940.
OMAi APPEEAY.
OrthoDBi EOG6K9QCV.
PhylomeDBi P59846.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
BioCyci TTHE300852:GH8R-298-MONOMER.

Miscellaneous databases

EvolutionaryTracei P59846.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action."
    Goto M., Nakajima Y., Hirotsu K.
    J. Biol. Chem. 277:15890-15896(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH ATP AND SUBSTRATE ANALOGS, SUBUNIT.
  3. "Structures of argininosuccinate synthetase in enzyme-ATP substrates and enzyme-AMP product forms: stereochemistry of the catalytic reaction."
    Goto M., Omi R., Miyahara I., Sugahara M., Hirotsu K.
    J. Biol. Chem. 278:22964-22971(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH ATP AND SUBSTRATES, SUBUNIT.

Entry informationi

Entry nameiASSY_THET8
AccessioniPrimary (citable) accession number: P59846
Secondary accession number(s): Q5SLK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3