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P59785

- ALGA_PSEFL

UniProt

P59785 - ALGA_PSEFL

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Protein

Alginate biosynthesis protein AlgA

Gene
algA
Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.

Cofactori

Cobalt (for PMI) By similarity.

Pathwayi

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mannose-1-phosphate guanylyltransferase activity Source: UniProtKB-EC
  3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. alginic acid biosynthetic process Source: UniProtKB-KW
  2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

Cobalt, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
UPA00126; UER00930.

Names & Taxonomyi

Protein namesi
Recommended name:
Alginate biosynthesis protein AlgA
Including the following 2 domains:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
Alternative name(s):
GDP-mannose pyrophosphorylase
Short name:
GMP
Short name:
GMPP
GTP--mannose-1-phosphate guanylyltransferase
Gene namesi
Name:algA
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Alginate biosynthesis protein AlgAPRO_0000194249Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP59785.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.

Sequencei

Sequence statusi: Complete.

P59785-1 [UniParc]FASTAAdd to Basket

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MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL ERLVFEGMDT    50
PIVVCNKDHR FIVNEQLANR KLECQRILME PFGRNTAPAV ALTAMMLVNE 100
GRDELMLVLP ADHVIDDQKA LQRALALATV AAERGEMVLF GVPATRPETG 150
YGYIKSTNDS LLPEGVSRVQ QFVEKPDEKR AVEFVKSGGY FWNSGMFLFR 200
ASRFLEELKK HDPDIYDTCV LTLERSEQTA DTVTLDDATF ACCPDNSIDY 250
AVMEKTQRAC VVPLSAGWSD VGCWASLWAV NDKDIHGNVS KGDVVIQDSR 300
NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVSTLNDQG 350
RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ISVKPGACLS LQMHHHRAEH 400
WIVVSGTAEV TCDENVFLLT ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS 450
GSYLGEDDIE RFEDIYGRST PVERGVSVKT IAQ 483
Length:483
Mass (Da):53,871
Last modified:July 25, 2003 - v1
Checksum:i27D861ACA527ECC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527790 Genomic DNA. Translation: AAP46700.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527790 Genomic DNA. Translation: AAP46700.1 .

3D structure databases

ProteinModelPortali P59785.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00423 .
UPA00126 ; UER00930 .

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProi IPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
Pfami PF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01479. GMP_PMI. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation."
    Gimmestad M., Sletta H., Ertesvaag H., Bakkevig K., Jain S., Suh S.-J., Skjaak-Braek G., Ellingsen T.E., Ohman D.E., Valla S.
    J. Bacteriol. 185:3515-3523(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 17397 / DSM 50091 / NCIMB 10525 / 12.

Entry informationi

Entry nameiALGA_PSEFL
AccessioniPrimary (citable) accession number: P59785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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