Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P59785 (ALGA_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alginate biosynthesis protein AlgA

Including the following 2 domains:

  1. Mannose-6-phosphate isomerase
    EC=5.3.1.8
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name=PMI
  2. Mannose-1-phosphate guanylyltransferase
    EC=2.7.7.13
    Alternative name(s):
    GDP-mannose pyrophosphorylase
    Short name=GMP
    Short name=GMPP
    GTP--mannose-1-phosphate guanylyltransferase
Gene names
Name:algA
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.

Catalytic activity

D-mannose 6-phosphate = D-fructose 6-phosphate.

GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.

Cofactor

Cobalt (for PMI) By similarity.

Pathway

Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.

Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Alginate biosynthesis protein AlgA
PRO_0000194249

Sequences

Sequence LengthMass (Da)Tools
P59785 [UniParc].

Last modified July 25, 2003. Version 1.
Checksum: 27D861ACA527ECC5

FASTA48353,871
        10         20         30         40         50         60 
MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL ERLVFEGMDT PIVVCNKDHR 

        70         80         90        100        110        120 
FIVNEQLANR KLECQRILME PFGRNTAPAV ALTAMMLVNE GRDELMLVLP ADHVIDDQKA 

       130        140        150        160        170        180 
LQRALALATV AAERGEMVLF GVPATRPETG YGYIKSTNDS LLPEGVSRVQ QFVEKPDEKR 

       190        200        210        220        230        240 
AVEFVKSGGY FWNSGMFLFR ASRFLEELKK HDPDIYDTCV LTLERSEQTA DTVTLDDATF 

       250        260        270        280        290        300 
ACCPDNSIDY AVMEKTQRAC VVPLSAGWSD VGCWASLWAV NDKDIHGNVS KGDVVIQDSR 

       310        320        330        340        350        360 
NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVSTLNDQG RSETQNHCEV 

       370        380        390        400        410        420 
YRPWGSYDSV DMGGRFQVKH ISVKPGACLS LQMHHHRAEH WIVVSGTAEV TCDENVFLLT 

       430        440        450        460        470        480 
ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS GSYLGEDDIE RFEDIYGRST PVERGVSVKT 


IAQ 

« Hide

References

[1]"The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation."
Gimmestad M., Sletta H., Ertesvaag H., Bakkevig K., Jain S., Suh S.-J., Skjaak-Braek G., Ellingsen T.E., Ohman D.E., Valla S.
J. Bacteriol. 185:3515-3523(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 17397 / DSM 50091 / NCIMB 10525 / 12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF527790 Genomic DNA. Translation: AAP46700.1.

3D structure databases

ProteinModelPortalP59785.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00126; UER00423.
UPA00126; UER00930.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsTIGR01479. GMP_PMI. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALGA_PSEFL
AccessionPrimary (citable) accession number: P59785
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways