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P59785

- ALGA_PSEFL

UniProt

P59785 - ALGA_PSEFL

Protein

Alginate biosynthesis protein AlgA

Gene

algA

Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (25 Jul 2003)
      Previous versions | rss
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    Functioni

    Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.

    Catalytic activityi

    D-mannose 6-phosphate = D-fructose 6-phosphate.
    GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.

    Cofactori

    Cobalt (for PMI).By similarity

    Pathwayi

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. mannose-1-phosphate guanylyltransferase activity Source: UniProtKB-EC
    3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. alginic acid biosynthetic process Source: UniProtKB-KW
    2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Alginate biosynthesis

    Keywords - Ligandi

    Cobalt, GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00126; UER00423.
    UPA00126; UER00930.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alginate biosynthesis protein AlgA
    Including the following 2 domains:
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name:
    PMI
    Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
    Alternative name(s):
    GDP-mannose pyrophosphorylase
    Short name:
    GMP
    Short name:
    GMPP
    GTP--mannose-1-phosphate guanylyltransferase
    Gene namesi
    Name:algA
    OrganismiPseudomonas fluorescens
    Taxonomic identifieri294 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Alginate biosynthesis protein AlgAPRO_0000194249Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP59785.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    3.90.550.10. 1 hit.
    InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
    IPR001538. Man6P_isomerase-2_C.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF01050. MannoseP_isomer. 1 hit.
    PF00483. NTP_transferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P59785-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL ERLVFEGMDT    50
    PIVVCNKDHR FIVNEQLANR KLECQRILME PFGRNTAPAV ALTAMMLVNE 100
    GRDELMLVLP ADHVIDDQKA LQRALALATV AAERGEMVLF GVPATRPETG 150
    YGYIKSTNDS LLPEGVSRVQ QFVEKPDEKR AVEFVKSGGY FWNSGMFLFR 200
    ASRFLEELKK HDPDIYDTCV LTLERSEQTA DTVTLDDATF ACCPDNSIDY 250
    AVMEKTQRAC VVPLSAGWSD VGCWASLWAV NDKDIHGNVS KGDVVIQDSR 300
    NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVSTLNDQG 350
    RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ISVKPGACLS LQMHHHRAEH 400
    WIVVSGTAEV TCDENVFLLT ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS 450
    GSYLGEDDIE RFEDIYGRST PVERGVSVKT IAQ 483
    Length:483
    Mass (Da):53,871
    Last modified:July 25, 2003 - v1
    Checksum:i27D861ACA527ECC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF527790 Genomic DNA. Translation: AAP46700.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF527790 Genomic DNA. Translation: AAP46700.1 .

    3D structure databases

    ProteinModelPortali P59785.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00423 .
    UPA00126 ; UER00930 .

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    3.90.550.10. 1 hit.
    InterProi IPR006375. Man1P_GuaTrfase/Man6P_Isoase.
    IPR001538. Man6P_isomerase-2_C.
    IPR005835. NTP_transferase.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    Pfami PF01050. MannoseP_isomer. 1 hit.
    PF00483. NTP_transferase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51182. SSF51182. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01479. GMP_PMI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation."
      Gimmestad M., Sletta H., Ertesvaag H., Bakkevig K., Jain S., Suh S.-J., Skjaak-Braek G., Ellingsen T.E., Ohman D.E., Valla S.
      J. Bacteriol. 185:3515-3523(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 17397 / DSM 50091 / NCIMB 10525 / 12.

    Entry informationi

    Entry nameiALGA_PSEFL
    AccessioniPrimary (citable) accession number: P59785
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2003
    Last sequence update: July 25, 2003
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3