P59785 (ALGA_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate biosynthesis protein AlgA

Including the following 2 domains:

1. Mannose-6-phosphate isomerase
   EC=5.3.1.8
   Alternative name(s):
   Phosphohexomutase
   Phosphomannose isomerase
   Short name=PMI
2. Mannose-1-phosphate guanylyltransferase
   EC=2.7.7.13
   Alternative name(s):
   GDP-mannose pyrophosphorylase
   Short name=GMP
   Short name=GMPP
   GTP--mannose-1-phosphate guanylyltransferase

Gene names

Name:

algA

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate. GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Cofactor	Cobalt (for PMI) By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Alginate biosynthesis
Ligand	Cobalt GTP-binding Nucleotide-binding
Molecular function	Isomerase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	GDP-mannose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway alginic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Alginate biosynthesis protein AlgA		PRO_0000194249

Sequences

	Sequence		Length	Mass (Da)
	P59785 [UniParc]. Last modified July 25, 2003. Version 1. Checksum: 27D861ACA527ECC5	FASTA	483	53,871
	10 20 30 40 50 60 MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL ERLVFEGMDT PIVVCNKDHR 70 80 90 100 110 120 FIVNEQLANR KLECQRILME PFGRNTAPAV ALTAMMLVNE GRDELMLVLP ADHVIDDQKA 130 140 150 160 170 180 LQRALALATV AAERGEMVLF GVPATRPETG YGYIKSTNDS LLPEGVSRVQ QFVEKPDEKR 190 200 210 220 230 240 AVEFVKSGGY FWNSGMFLFR ASRFLEELKK HDPDIYDTCV LTLERSEQTA DTVTLDDATF 250 260 270 280 290 300 ACCPDNSIDY AVMEKTQRAC VVPLSAGWSD VGCWASLWAV NDKDIHGNVS KGDVVIQDSR 310 320 330 340 350 360 NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVSTLNDQG RSETQNHCEV 370 380 390 400 410 420 YRPWGSYDSV DMGGRFQVKH ISVKPGACLS LQMHHHRAEH WIVVSGTAEV TCDENVFLLT 430 440 450 460 470 480 ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS GSYLGEDDIE RFEDIYGRST PVERGVSVKT IAQ

References

[1]

"The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation." Gimmestad M., Sletta H., Ertesvaag H., Bakkevig K., Jain S., Suh S.-J., Skjaak-Braek G., Ellingsen T.E., Ohman D.E., Valla S. J. Bacteriol. 185:3515-3523(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCIMB 10525.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF527790 Genomic DNA. Translation: AAP46700.1.
3D structure databases
ProteinModelPortal	P59785.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00126; UER00423. UPA00126; UER00930.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGA_PSEFL

Accession

Primary (citable) accession number: P59785

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	July 25, 2003
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program