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P59785

- ALGA_PSEFL

UniProt

P59785 - ALGA_PSEFL

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Protein

Alginate biosynthesis protein AlgA

Gene

algA

Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics.

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.

Cofactori

Cobalt (for PMI).By similarity

Pathwayi

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. mannose-1-phosphate guanylyltransferase activity Source: UniProtKB-EC
  3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. alginic acid biosynthetic process Source: UniProtKB-KW
  2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

Cobalt, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00126; UER00423.
UPA00126; UER00930.

Names & Taxonomyi

Protein namesi
Recommended name:
Alginate biosynthesis protein AlgA
Including the following 2 domains:
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name:
PMI
Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
Alternative name(s):
GDP-mannose pyrophosphorylase
Short name:
GMP
Short name:
GMPP
GTP--mannose-1-phosphate guanylyltransferase
Gene namesi
Name:algA
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Alginate biosynthesis protein AlgAPRO_0000194249Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP59785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.

Sequencei

Sequence statusi: Complete.

P59785 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL ERLVFEGMDT
60 70 80 90 100
PIVVCNKDHR FIVNEQLANR KLECQRILME PFGRNTAPAV ALTAMMLVNE
110 120 130 140 150
GRDELMLVLP ADHVIDDQKA LQRALALATV AAERGEMVLF GVPATRPETG
160 170 180 190 200
YGYIKSTNDS LLPEGVSRVQ QFVEKPDEKR AVEFVKSGGY FWNSGMFLFR
210 220 230 240 250
ASRFLEELKK HDPDIYDTCV LTLERSEQTA DTVTLDDATF ACCPDNSIDY
260 270 280 290 300
AVMEKTQRAC VVPLSAGWSD VGCWASLWAV NDKDIHGNVS KGDVVIQDSR
310 320 330 340 350
NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVSTLNDQG
360 370 380 390 400
RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ISVKPGACLS LQMHHHRAEH
410 420 430 440 450
WIVVSGTAEV TCDENVFLLT ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS
460 470 480
GSYLGEDDIE RFEDIYGRST PVERGVSVKT IAQ
Length:483
Mass (Da):53,871
Last modified:July 25, 2003 - v1
Checksum:i27D861ACA527ECC5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527790 Genomic DNA. Translation: AAP46700.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF527790 Genomic DNA. Translation: AAP46700.1 .

3D structure databases

ProteinModelPortali P59785.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00423 .
UPA00126 ; UER00930 .

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProi IPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
Pfami PF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01479. GMP_PMI. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation."
    Gimmestad M., Sletta H., Ertesvaag H., Bakkevig K., Jain S., Suh S.-J., Skjaak-Braek G., Ellingsen T.E., Ohman D.E., Valla S.
    J. Bacteriol. 185:3515-3523(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 17397 / DSM 50091 / NCIMB 10525 / 12.

Entry informationi

Entry nameiALGA_PSEFL
AccessioniPrimary (citable) accession number: P59785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: October 1, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3