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Protein

Dedicator of cytokinesis protein 4

Gene

Dock4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP (By similarity).By similarity

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
  2. PDZ domain binding Source: MGI
  3. Rac GTPase binding Source: MGI
  4. receptor tyrosine kinase binding Source: MGI

GO - Biological processi

  1. cell chemotaxis Source: UniProtKB
  2. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 4
Gene namesi
Name:Dock4
Synonyms:Kiaa0716
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1918006. Dock4.

Subcellular locationi

  1. Endomembrane system By similarity; Peripheral membrane protein By similarity
  2. Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. endomembrane system Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB
  4. stereocilium Source: HGNC
  5. stereocilium bundle Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Dock4 are involved in the cause of some cancers, which are probably due to lack of Rap1 activation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19781978Dedicator of cytokinesis protein 4PRO_0000189991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671Phosphotyrosine1 Publication
Modified residuei1623 – 16231PhosphoserineBy similarity
Modified residuei1627 – 16271PhosphoserineBy similarity
Modified residuei1629 – 16291PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP59764.
PaxDbiP59764.
PRIDEiP59764.

PTM databases

PhosphoSiteiP59764.

Expressioni

Gene expression databases

BgeeiP59764.
CleanExiMM_DOCK4.
GenevestigatoriP59764.

Interactioni

Subunit structurei

Interacts with the SH3 domain of CRK. Interacts with nucleotide-free Rap1. Interacts with FASLG. Interacts with ELMO2 and EPHA2; mediates activation of RAC1 by EPHA2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP59764.
SMRiP59764. Positions 2-164, 401-576, 1187-1593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6762SH3PROSITE-ProRule annotationAdd
BLAST
Domaini401 – 574174DHR-1Add
BLAST
Domaini1199 – 1605407DHR-2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1797 – 18037SH3-bindingSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1642 – 1863222Ser-richAdd
BLAST
Compositional biasi1875 – 196086Pro-richAdd
BLAST

Domaini

The DHR-2 domain probably mediates the GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.Curated
Contains 1 DHR-1 domain.Curated
Contains 1 DHR-2 domain.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiNOG289808.
GeneTreeiENSGT00610000085806.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiP59764.
KOiK17697.
OMAiMMGCEIF.
OrthoDBiEOG7QNVK8.
PhylomeDBiP59764.
TreeFamiTF300423.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PfamiPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59764-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWIPTEHEKY GVVIASFRGT VPYGLSLEIG DTVQILEKCD GWYRGFALKN
60 70 80 90 100
PNIKGIFPSS YVHLKNACVK NKGQFEMVIP TEDSVITEMT STLRDWGTMW
110 120 130 140 150
KQLYVRNEGD LFHRLWHIMN EILDLRRQVL VGHLTHDRMK DVKRHITARL
160 170 180 190 200
DWGNEQLGLD LVPRKEYAMV DPEDISITEL YRLMEHRHRK KDTPVQASSH
210 220 230 240 250
HLFVQMKSLM CSNLGEELEV IFSLFDSKEN RPISERFFLR LNRNGLPKAP
260 270 280 290 300
DKPERHCSLF VDLGSSELRK DIYITVHIIR IGRMGAGEKK NACSVQYRRP
310 320 330 340 350
FGCAVLSIAD LLTGETKDDL VLKVYMCNTE SEWYQIHENI IKKLNARYNL
360 370 380 390 400
TGSNAGLAVS LQLLHGDIEQ IRREYSSVFS HGVSITRKLG FSDIIMPGEM
410 420 430 440 450
RNDLYITVER GEFEKGGKSV ARNVEVTMFI VDSNGQPLKD FISFGSGEPP
460 470 480 490 500
ASEYHSFVLY HNNSPRWSEL LKLPIPVDKF RGSHIRFEFR HCSTKEKGEK
510 520 530 540 550
KLFGFSFVPL MQEDGRTLPD GTHELIVHKC EENTNLQDTT RYLKLPFSKV
560 570 580 590 600
IFLGNNNQTM KATKESFWIT SFLCSTKLTQ NGDMLDLLKW RTHPDKITGC
610 620 630 640 650
LSKLKEIDGS EIVKFLQDTL DTLFGILDEN SQKYGSKVFD SLVHIINLLQ
660 670 680 690 700
DSKFHHFKPV MDTYIESHFA GALAYRDLIK VLKWYVDRIT EAERQEHIQE
710 720 730 740 750
VLKAQEYIFK YIVQSRRLFS LATGGQNEEE FRCCIQELLM SVRFFLSQES
760 770 780 790 800
KGTGALSQSQ AVFLSSFPAV YSELLKLFDV REVANLVQDT LGSLPTIMHV
810 820 830 840 850
DDSLQAIKLQ CIGKTVESQL YTNPDSRYIL LPVVLHHLHI HLQEQKDLIM
860 870 880 890 900
CARILSNVFC LIKKNSSEKS VLEEIDVIVA SLLDILLRTI LEITSRPQAS
910 920 930 940 950
SSAMRLQFQD VTGEFVACLL SLLRQMTDRH YQQLLNSFST KEELRDFLLQ
960 970 980 990 1000
IFTVFRILIR PEMFPKDWTV MRLVANNVII TTVLYLSDAL RKNFLNENFD
1010 1020 1030 1040 1050
YKIWDSYFYL AVIFINQLCL QLEMFTPSKK KKVLEKYGDM RVTMGCEIFS
1060 1070 1080 1090 1100
MWQNLGEHKL HFIPALIGPF LEVTLIPQPD LRNVMIPIFH DMMDWEQRRS
1110 1120 1130 1140 1150
GNFKQVEAKL IDKLDSLMSE GKGDETYREL FNSIIPLFGP YPSLLKKIER
1160 1170 1180 1190 1200
ETWRESGVSL IATVTRLMER LLDYRDCMKI GEVDGKKIGC TVSLLNFYKT
1210 1220 1230 1240 1250
ELNKEEMYIR YIHKLYDLHL KAQNFTEAAY TLLLYDELLE WSDRPLREFL
1260 1270 1280 1290 1300
TYPMQTEWQR KEHLHLTIIQ NFDRGKCWEN GIILCRKIAE QYESYYDYRN
1310 1320 1330 1340 1350
LSKMRMMEAS LYDKIMDQQR LEPEFFRVGF YGKKFPFFLR NKEFVCRGHD
1360 1370 1380 1390 1400
YERLEAFQQR MLNEFPHAIA MQHANQPDET IFQAEAQYLQ IYAVTPIPES
1410 1420 1430 1440 1450
QEVLQREGVP DNIKSFYKVN HIWKFRYDRP FHKGAKDKEN EFKSLWVERT
1460 1470 1480 1490 1500
SLYLVQSLPG ISRWFEVEKR EVVEMSPLEN AIEVLENKNQ QLKTLISQCQ
1510 1520 1530 1540 1550
TRQMQNINPL TMCLNGVIDA AVNGGVSRYQ EAFFVKDYIL SHPEDGEKIA
1560 1570 1580 1590 1600
RLRELMLEQA QILEFGLAVH EKFVPQDMRP LHKKLVDQFF VMKSSFGIQE
1610 1620 1630 1640 1650
FPACIQASPV HFPNGSPRVC RNSAPASMSP DGTRVIPRRS PLSYPAVNRY
1660 1670 1680 1690 1700
SSSSLSSQAS AEVSNITGQS ESSDEVFNMQ PSPSTSSLSS THSASPNVTS
1710 1720 1730 1740 1750
SAPSSARASP LLSDKHKHSR ENSCLSPRDR PCSAIYPTPV EPSQRMLFNH
1760 1770 1780 1790 1800
IGDGALPRSD PNLSAPEKAV NPTPSSWSLD SGKEAKNMSD SGKLISPPVP
1810 1820 1830 1840 1850
PRPTQTASPA RHTTSVSPSP AGRSPLKGSV QSFTPSPVEY NSPGLSSNSP
1860 1870 1880 1890 1900
VLSGSYSSGI SSLSRCSTSE TSGFENQANE QSVPVPVPVP VPVPVPSFSG
1910 1920 1930 1940 1950
SEEPVRKESK TPPPYSVYER TLRRPVPLPH SLSIPVTSEP PALPPKPLAA
1960 1970
RSSHLENGTR RTEPGPRPRP LPRKVSQL
Length:1,978
Mass (Da):226,550
Last modified:July 3, 2003 - v1
Checksum:i4A1FC9C71C5A5719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122353 mRNA. Translation: BAC65635.1.
CCDSiCCDS25895.1.
RefSeqiNP_766391.2. NM_172803.2.
UniGeneiMm.341423.

Genome annotation databases

EnsembliENSMUST00000037488; ENSMUSP00000047387; ENSMUSG00000035954.
GeneIDi238130.
KEGGimmu:238130.
UCSCiuc007nlh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122353 mRNA. Translation: BAC65635.1.
CCDSiCCDS25895.1.
RefSeqiNP_766391.2. NM_172803.2.
UniGeneiMm.341423.

3D structure databases

ProteinModelPortaliP59764.
SMRiP59764. Positions 2-164, 401-576, 1187-1593.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP59764.

Proteomic databases

MaxQBiP59764.
PaxDbiP59764.
PRIDEiP59764.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037488; ENSMUSP00000047387; ENSMUSG00000035954.
GeneIDi238130.
KEGGimmu:238130.
UCSCiuc007nlh.1. mouse.

Organism-specific databases

CTDi9732.
MGIiMGI:1918006. Dock4.
RougeiSearch...

Phylogenomic databases

eggNOGiNOG289808.
GeneTreeiENSGT00610000085806.
HOGENOMiHOG000006631.
HOVERGENiHBG051389.
InParanoidiP59764.
KOiK17697.
OMAiMMGCEIF.
OrthoDBiEOG7QNVK8.
PhylomeDBiP59764.
TreeFamiTF300423.

Miscellaneous databases

NextBioi383672.
PROiP59764.
SOURCEiSearch...

Gene expression databases

BgeeiP59764.
CleanExiMM_DOCK4.
GenevestigatoriP59764.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PfamiPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. Cited for: DISEASE.
  3. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-167, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiDOCK4_MOUSE
AccessioniPrimary (citable) accession number: P59764
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: July 3, 2003
Last modified: April 29, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.