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Protein

MKL/myocardin-like protein 2

Gene

Mkl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • transcription coactivator activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • blood vessel morphogenesis Source: MGI
  • cardiac muscle tissue development Source: MGI
  • embryonic organ development Source: MGI
  • forebrain development Source: MGI
  • gene expression Source: MGI
  • heart development Source: MGI
  • heart morphogenesis Source: MGI
  • in utero embryonic development Source: MGI
  • liver development Source: MGI
  • muscle organ development Source: UniProtKB-KW
  • neuron migration Source: MGI
  • neuron projection development Source: MGI
  • positive regulation of striated muscle tissue development Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of gene expression Source: MGI
  • smooth muscle cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
MKL/myocardin-like protein 2
Alternative name(s):
Myocardin-related transcription factor B
Short name:
MRTF-B
Gene namesi
Name:Mkl2
Synonyms:Mrtfb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:3050795. Mkl2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10801080MKL/myocardin-like protein 2PRO_0000126629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei66 – 661PhosphoserineCombined sources
Modified residuei531 – 5311PhosphoserineCombined sources
Modified residuei535 – 5351PhosphoserineBy similarity
Modified residuei913 – 9131PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP59759.
MaxQBiP59759.
PaxDbiP59759.
PRIDEiP59759.

PTM databases

iPTMnetiP59759.
PhosphoSiteiP59759.

Expressioni

Tissue specificityi

Widely expressed. High expression in heart, brain and testis. Lower expression in lung, liver and kidney.1 Publication

Developmental stagei

Detected throughout the embryo at 10.5 dpc. High expression in epithelial cells of the lung, kidney, bladder, colon, testis, in the smooth muscle of the colon and small intestines, and in the mesenchymal cells adjacent to the olfactory epithelium at 15.5 dpc.1 Publication

Gene expression databases

CleanExiMM_MKL2.

Interactioni

Subunit structurei

Interacts with MKL1 and SRF.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI

Protein-protein interaction databases

BioGridi232120. 2 interactions.
DIPiDIP-60885N.
STRINGi10090.ENSMUSP00000009713.

Structurei

3D structure databases

ProteinModelPortaliP59759.
SMRiP59759. Positions 39-151, 375-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati40 – 6526RPEL 1Add
BLAST
Repeati84 – 10926RPEL 2Add
BLAST
Repeati128 – 15326RPEL 3Add
BLAST
Domaini383 – 41735SAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni557 – 58529Required for interaction with itself and with MKL1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili539 – 59456Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi320 – 34627Gln-richAdd
BLAST
Compositional biasi665 – 806142Gln-richAdd
BLAST

Domaini

The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.By similarity

Sequence similaritiesi

Contains 3 RPEL repeats.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IFJX. Eukaryota.
ENOG41101AM. LUCA.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiP59759.
PhylomeDBiP59759.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR004018. RPEL_repeat.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF02755. RPEL. 2 hits.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDSSKKQPQ GFPEILTAED FEPFKEKECL EGSNQKSLKE VLQLRLQQRR
60 70 80 90 100
TREQLVDQGI MPPLKSPAAF HEQIKSLERA RTENFLKHKI RSRPDRSELV
110 120 130 140 150
RMHILEETFA EPSLQATQMK LKRARLADDL NEKIAQRPGP MELVEKNILP
160 170 180 190 200
VDSSVKEAII GVVKEDYPHT HGEFSFDEDS SDALSPDQPA SQESQGSAAS
210 220 230 240 250
PSEPKVSASP PPVTASTPAQ FTSVSPAVPE FLKTPLTADQ PPTRSTAPVL
260 270 280 290 300
PTNTVSSAKS GPMLVKQSHP KNPNDKHRSK KCKDPKPRVK KLKYHQYIPP
310 320 330 340 350
NQKGEKSEPQ MDSNYARLLQ QQQLFLQLQI LSQQQQQQQQ QHYNYQTILP
360 370 380 390 400
APIKTDKNSS SGSNSGSSSS MPARRPGPLP SSLDDLKVSE LKTELKLRGL
410 420 430 440 450
PVSGTKPDLI ERLKPYQEVT SSNLATGSIV AVSSATIVTS NPEVTVALPV
460 470 480 490 500
TTLHNAVTSS VSTFKADLAL PATSSVPHVE NAHSPLPISP SPSEQSSLST
510 520 530 540 550
DDTNMTDTFT EIMTMMSPSQ LLCSSPLRVV SHDDSLSPSS STLSTLELDA
560 570 580 590 600
AEKDRKLQEK EKQIEELKRK LEQEQKLVEV LKMQLEVEKR GQQRPPDPQP
610 620 630 640 650
SDPPHPFNTS DPKHGSVGSS IKDEASLPDC SSPQQPITVP GHSVGQPIST
660 670 680 690 700
GSQTLVAKKT VVVKQEVPMA QAEQQNVVSQ FYLSSQGQPP ALVAQPQALL
710 720 730 740 750
TTQTTQLLLP VSIQGSNVTS VQLPVGSLQL QTPAQGRVQA QPHVAAATQV
760 770 780 790 800
PAAALPSALT SALPQKQEAF PQHVLGQPQP VRKVFTNSAP NTVLQYQRQP
810 820 830 840 850
GPTNQQPFVS KTSNPALQSR TAPLAPLQNG PSLASKPSSP PPPQQFVVQH
860 870 880 890 900
SLFATPITKT KDPPRYEEAI KQTRSTQPAL PEVSSVHSQQ MDDLFDILIK
910 920 930 940 950
SGEISFPIKE EPSPISKMKP VTASITTMPV NTVVSRPPPQ VQIAPPVSLE
960 970 980 990 1000
PVNSLSASLE NQLEAFLDGT LPSATDTGPL QNSSEDRESF SLIEDLQNDL
1010 1020 1030 1040 1050
LSHSSMLYQS HSPMETSEAQ LVSGTPCLSL DLSDSNLDNM EWLDITMPTT
1060 1070 1080
SSGLTPLSTT APSMFSADFL DPQDLPLPWD
Length:1,080
Mass (Da):117,547
Last modified:June 27, 2003 - v1
Checksum:i1781D8EF34517DAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532598 mRNA. Translation: AAN33042.1.
CCDSiCCDS37258.1.
RefSeqiNP_705816.2. NM_153588.3.
UniGeneiMm.270643.

Genome annotation databases

GeneIDi239719.
KEGGimmu:239719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532598 mRNA. Translation: AAN33042.1.
CCDSiCCDS37258.1.
RefSeqiNP_705816.2. NM_153588.3.
UniGeneiMm.270643.

3D structure databases

ProteinModelPortaliP59759.
SMRiP59759. Positions 39-151, 375-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232120. 2 interactions.
DIPiDIP-60885N.
STRINGi10090.ENSMUSP00000009713.

PTM databases

iPTMnetiP59759.
PhosphoSiteiP59759.

Proteomic databases

EPDiP59759.
MaxQBiP59759.
PaxDbiP59759.
PRIDEiP59759.

Protocols and materials databases

DNASUi239719.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi239719.
KEGGimmu:239719.

Organism-specific databases

CTDi57496.
MGIiMGI:3050795. Mkl2.

Phylogenomic databases

eggNOGiENOG410IFJX. Eukaryota.
ENOG41101AM. LUCA.
HOGENOMiHOG000038001.
HOVERGENiHBG036493.
InParanoidiP59759.
PhylomeDBiP59759.

Miscellaneous databases

NextBioi384249.
PROiP59759.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MKL2.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR004018. RPEL_repeat.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF02755. RPEL. 2 hits.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00707. RPEL. 3 hits.
SM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51073. RPEL. 3 hits.
PS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Potentiation of serum response factor activity by a family of myocardin-related transcription factors."
    Wang D.-Z., Li S., Hockemeyer D., Sutherland L., Wang Z., Schratt G., Richardson J.A., Nordheim A., Olson E.N.
    Proc. Natl. Acad. Sci. U.S.A. 99:14855-14860(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH SRF.
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry."
    Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  3. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66 AND SER-531, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiMKL2_MOUSE
AccessioniPrimary (citable) accession number: P59759
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: June 27, 2003
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.