Reviewed, UniProtKB/Swiss-Prot P59736 (AROH_SHIFL)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthetase

Gene names

Name:	aroH
Ordered Locus Names:	SF1526, S1644

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) By similarity.
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Sequence similarities	Belongs to the class-I DAHP synthetase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		PRO_0000140846

Sequences

	Sequence		Length	Mass (Da)
	P59736-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 02C7DC9F85B23D8B	FASTA	348	38,823
	10 20 30 40 50 60 MNRTDELRTA RIESLVTPAE LALRYPVTPG VATHVTDSRR RIEKILNGED KRLLVIIGPC 70 80 90 100 110 120 SIHDLTAAME YATRLQSLRN QYQSRLEIVM RTYFEKPRTV VGWKGLISDP DLNGSYRVNH 130 140 150 160 170 180 GLELARKLLL QVNELGVPTA TEFLDMVTGQ FIADLISWGA IGARTTESQI HREMASALSC 190 200 210 220 230 240 PVGFKNGTDG NTRIAVDAIR AARASHMFLS PDKNGQMTIY QTSGNPYGHI IMRGGKKPNY 250 260 270 280 290 300 HADDIAAACD TLHEFDLPEH LVVDFSHGNC QKQHRRQLEV CEDICQQIRN GSTEIAGIMA 310 320 330 340 ESFLREGTQK IVGGQPLTYG QSITDPCLGW EDTERLVEKL AFAVDTRF

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN43116.1. AE014073 Genomic DNA. Translation: AAP17007.1.
RefSeq	NP_707409.1. NP_837200.1.
3D structure databases
HSSP	HSSP built from PDB template 1GG1 based on UniProtKB P00886.
ModBase	Search...
Genome annotation databases
GeneID	1024719. 1079648.
GenomeReviews	Gene locus SF1526 in contig AE005674_GR. Gene locus S1644 in contig AE014073_GR.
KEGG	sfl:SF1526. sfx:S1644.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59736.
OMA	ELFIVMR.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN43116.1-MON.
BRENDA	2.5.1.54. 189495.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. aroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROH_SHIFL

Accession

Primary (citable) accession number: P59736

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)