Reviewed, UniProtKB/Swiss-Prot P59723 (HIF1N_DANRE)

Names and origin

Protein names

Recommended name:
    Hypoxia-inducible factor 1-alpha inhibitor
    EC=1.14.11.16
Alternative name(s):
    Hypoxia-inducible factor asparagine hydroxylase

Gene names

Name:

hif1an

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydroxylates HIF-1 alpha in the C-terminal transactivation domain (CAD). The hydroxylation prevents interaction of HIF-1 with transcriptional coactivators By similarity.
Catalytic activity	Peptide L-aspartate + 2-oxoglutarate + O2 = peptide 3-hydroxy-L-aspartate + succinate + CO2.
Cofactor	Iron By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 JmjC domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW peptide-aspartate beta-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Hypoxia-inducible factor 1-alpha inhibitor		PRO_0000083973
Regions
	Domain	133 – 303	171	JmjC
Sites
	Metal binding	190	1	Iron; catalytic By similarity
	Metal binding	192	1	Iron; catalytic By similarity
	Metal binding	270	1	Iron; catalytic By similarity
	Binding site	136	1	2-oxoglutarate By similarity
	Binding site	187	1	2-oxoglutarate By similarity
	Binding site	205	1	2-oxoglutarate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59723-1 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: FA9E2DC8B173B64C	FASTA	344	39,876
	10 20 30 40 50 60 MAETDGAAAF TELRDPGWDG SQLRQYTFPT RQIPRLSHTD PRAEVLINNE EPVVLTDTSL 70 80 90 100 110 120 VYPALKWDIP YLQENIGNGD FSVYIAENHK FLYYDEKKMV NFQDFVPKSR RIEMKFSEFV 130 140 150 160 170 180 DKMHQTEEQG GKGRVYLQQT LNDTVGRKIV VDFLGFNWNW INKQQAKRNW GPLTSNLLLI 190 200 210 220 230 240 GMEGNVTPAH YDEQQNFFAQ IKGHKRCILF PPEQFDCLYP YPVHHPCDRQ SQVDFENPDY 250 260 270 280 290 300 DKFPNFKNAV GYEAVVGPGD VLYIPMYWWH HIESLLNGGE TITVNFWYKG APTPKRIEYP 310 320 330 340 LKAHQKVAIM RNIEKMLGEA LGDPHEVGPL LNMMIKGRYD HGLS

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC044475 mRNA. Translation: AAH44475.1.
IPI	IPI00512392.
RefSeq	NP_958904.1.
UniGene	Dr.13371
3D structure databases
HSSP	HSSP built from PDB template 1MZE based on UniProtKB Q9NWT6.
SMR	P59723. Positions 16-340.
ModBase	Search...
Proteomic databases
PRIDE	P59723.
Genome annotation databases
Ensembl	ENSDART00000042049; ENSDARP00000042048; ENSDARG00000031915; Danio rerio. [Genome view]
GeneID	373126.
KEGG	dre:373126.
Organism-specific databases
CTD	373126.
ZFIN	ZDB-GENE-030826-19. hif1an.
Phylogenomic databases
HOGENOM	P59723.
HOVERGEN	P59723.
Enzyme and pathway databases
BRENDA	1.14.11.16. 96826.
Gene expression databases
ArrayExpress	P59723.
Bgee	P59723.
Family and domain databases
InterPro	IPR003347. TF_JmjC_AAH. [Graphical view]
SMART	SM00558. JmjC. 1 hit. [Graphical view]
PROSITE	PS51184. JMJC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIF1N_DANRE

Accession

Primary (citable) accession number: P59723

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	September 22, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project