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P59721

- TRMB_STRAW

UniProt

P59721 - TRMB_STRAW

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, SAV_4115
Organism
Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041S-adenosyl-L-methionine By similarity
Binding sitei129 – 1291S-adenosyl-L-methionine By similarity
Binding sitei156 – 1561S-adenosyl-L-methionine By similarity
Active sitei179 – 1791 By similarity
Binding sitei179 – 1791S-adenosyl-L-methionine By similarity
Binding sitei183 – 1831Substrate By similarity
Binding sitei215 – 2151Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSAVE227882:GJU1-4148-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:SAV_4115
    OrganismiStreptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680)
    Taxonomic identifieri227882 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces
    ProteomesiUP000000428: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 290290tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000171400Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi227882.SAV_4115.

    Structurei

    3D structure databases

    ProteinModelPortaliP59721.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni252 – 2554Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiLHIATDH.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59721-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDSLHTPEE PRPGPGEQLA HAHDGSLRHT RAKGEPRFPD GPKADPAGSH    50
    FERRIRSFQP RRSRVTAGQA DALQRLWPKW GLDIDGHALD LTELFGNTHP 100
    VVLEIGFGMG EATARMAAED PDTGILAVDV HTPGQGNLLN LADQHGLTNI 150
    RVANGDAIIL LREMLPPDSL DGLRVYFPDP WPKKRHHKRR LIQPEFLTLA 200
    ATRLRPGALV HCATDWEPYA EQMLDVLTAH PDFENTQPTG GYAPRPGFRP 250
    LTRFEGQGLD KGHVVNDLLF RRVQPRDQHR DLPPSATEAD 290
    Length:290
    Mass (Da):32,245
    Last modified:June 16, 2003 - v1
    Checksum:i65AAD0259AE53385
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000030 Genomic DNA. Translation: BAC71827.1.
    RefSeqiNP_825292.1. NC_003155.4.

    Genome annotation databases

    EnsemblBacteriaiBAC71827; BAC71827; SAV_4115.
    GeneIDi1215033.
    KEGGisma:SAV_4115.
    PATRICi23721977. VBIStrAve112782_4387.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000030 Genomic DNA. Translation: BAC71827.1 .
    RefSeqi NP_825292.1. NC_003155.4.

    3D structure databases

    ProteinModelPortali P59721.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 227882.SAV_4115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC71827 ; BAC71827 ; SAV_4115 .
    GeneIDi 1215033.
    KEGGi sma:SAV_4115.
    PATRICi 23721977. VBIStrAve112782_4387.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi LHIATDH.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci SAVE227882:GJU1-4148-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites."
      Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M., Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T., Sakaki Y., Hattori M.
      Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.
    2. "Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis."
      Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T., Sakaki Y., Hattori M., Omura S.
      Nat. Biotechnol. 21:526-531(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 31267 / DSM 46492 / JCM 5070 / NCIMB 12804 / NRRL 8165 / MA-4680.

    Entry informationi

    Entry nameiTRMB_STRAW
    AccessioniPrimary (citable) accession number: P59721
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: June 11, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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