P59716 (TRMB_BACAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	BA_4950, GBAA_4950, BAS4594

Organism

Bacillus anthracis [Reference proteome] [HAMAP]

Taxonomic identifier

1392 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171288
Regions
	Region	191 – 194	4	Substrate binding Potential
Sites
	Binding site	44	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	S-adenosyl-L-methionine By similarity
	Binding site	118	1	S-adenosyl-L-methionine By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	154	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P59716 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: 3227646A0B193686	FASTA	217	25,600
	10 20 30 40 50 60 MRLRHKPYAM DRINEYSHIV IGNPEERAGN WKEVFGNEQP IHIEVGTGRG RFMYDMAKAN 70 80 90 100 110 120 PHINYIGIEK FTSVVVDALD KLIEEELPNL KLINKDAEDL TVFFAKGEID RVYLNFSDPW 130 140 150 160 170 180 PKKRHTKRRL TYKTFLRNYE EVLVEGGEIH FKTDNQGLFE YSLMSMAEYG MLLTYLSLDL 190 200 210 HNSDFEGNIM TEYEEKFSSK GHRIYRVEAK YRTEPMQ

References

[1]	"The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria." Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J. , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M. Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames / isolate Porton.
[2]	"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'." Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D., Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A. J. Bacteriol. 191:445-446(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ames ancestor.
[3]	"Complete genome sequence of Bacillus anthracis Sterne." Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sterne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016879 Genomic DNA. Translation: AAP28633.1. AE017334 Genomic DNA. Translation: AAT34070.1. AE017225 Genomic DNA. Translation: AAT56890.1.
RefSeq	NP_847147.1. NC_003997.3. YP_021595.1. NC_007530.2. YP_030840.1. NC_005945.1.
3D structure databases
ProteinModelPortal	P59716.
SMR	P59716. Positions 11-213.
ModBase	Search...
Protein-protein interaction databases
STRING	198094.BA_4950.
Protocols and materials databases
DNASU	1084155.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP28633; AAP28633; BA_4950. AAT34070; AAT34070; GBAA_4950. AAT56890; AAT56890; BAS4594.
GeneID	1084155. 2818757. 2850425.
KEGG	ban:BA_4950. bar:GBAA_4950. bat:BAS4594.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000251689.
KO	K03439.
OMA	RLRNKPW.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	BANT260799:GJAJ-4650-MONOMER. BANT261594:GJ7F-4808-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_BACAN

Accession

Primary (citable) accession number: P59716
Secondary accession number(s): Q6HS48, Q6KLE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program