Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P59709

- HEMA_CVBQ

UniProt

P59709 - HEMA_CVBQ

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Bovine coronavirus (strain Quebec) (BCoV) (BCV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (16 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401NucleophileBy similarity
    Active sitei326 – 3261Charge relay systemBy similarity
    Active sitei329 – 3291Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    ORF Names:2b
    OrganismiBovine coronavirus (strain Quebec) (BCoV) (BCV)
    Taxonomic identifieri11133 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000008572: Genome

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Chaini19 – 424406Hemagglutinin-esterasePRO_0000037143Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 65By similarity
    Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi89 – 891N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi113 ↔ 162By similarity
    Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi197 ↔ 276By similarity
    Disulfide bondi205 ↔ 249By similarity
    Glycosylationi236 – 2361N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi307 ↔ 312By similarity
    Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi347 ↔ 371By similarity
    Glycosylationi358 – 3581N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliP59709.
    SMRiP59709. Positions 19-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 392374Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini414 – 42411IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei393 – 41321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni7 – 127121Esterase domain first partBy similarityAdd
    BLAST
    Regioni128 – 266139Receptor bindingBy similarityAdd
    BLAST
    Regioni267 – 379113Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P59709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLLLRFVLV SCIIGSLGFD NPPTNVVSHL NGDWFLFGDS RSDCNHVVNT    50
    NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY 100
    EGLNFTPYHA FKCTTSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY 150
    VYNGSAQSTA LCKSGSLVLN NPAYIAREAN FGDYYYKVEA DFYLSGCDEY 200
    IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH 250
    YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD 300
    NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYDSPCFSQ 350
    QGVFRYDNVS SVWPLYSYGR CPTAADINTP DVPICVYDPL PLILLGILLG 400
    VAVIIIVVLL LYFMVDNGTR LHDA 424
    Length:424
    Mass (Da):47,709
    Last modified:June 16, 2003 - v1
    Checksum:iF1CE076E4AA7B277
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti5 – 51L → P in strain: Isolate BCQ.3, Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.2590, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
    Natural varianti8 – 81V → A in strain: Isolate BCQ.2590.
    Natural varianti11 – 111S → C in strain: Isolate BCQ.1523, Isolate BCQ.2442 and Isolate BCQ.2508.
    Natural varianti49 – 491N → T in strain: Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
    Natural varianti53 – 531R → P in strain: Isolate BCQ.2590.
    Natural varianti66 – 661D → G in strain: Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.3708 and Isolate BCQ.3994.
    Natural varianti103 – 1031L → I in strain: Isolate BCQ.2439.
    Natural varianti103 – 1031L → V in strain: Isolate BCQ.3, Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.2590, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
    Natural varianti114 – 1141T → I in strain: Isolate BCQ.3708.
    Natural varianti182 – 1821G → R in strain: Isolate BCQ.7373.
    Natural varianti245 – 2451F → L in strain: Isolate BCQ.7373.
    Natural varianti282 – 2821D → G in strain: Isolate BCQ.7373.
    Natural varianti344 – 3441D → A in strain: Isolate BCQ.2590.
    Natural varianti350 – 3501Q → R in strain: Isolate BCQ.2590.
    Natural varianti367 – 3671S → P in strain: Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.2590, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
    Natural varianti392 – 3921L → I in strain: Isolate BCQ.3, Isolate BCQ.376, Isolate BCQ.571, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38962 Genomic RNA. Translation: AAA92989.1.
    L38963 Genomic RNA. Translation: AAA92990.1.
    U06093 Genomic RNA. Translation: AAA92991.1.
    AF239306 Genomic RNA. Translation: AAG40594.1.
    AF239307 Genomic RNA. Translation: AAG40600.1.
    AF230523 Genomic RNA. Translation: AAG40588.1.
    AF230524 Genomic RNA. Translation: AAG40589.1.
    AF230525 Genomic RNA. Translation: AAG40590.1.
    AF230526 Genomic RNA. Translation: AAG40591.1.
    AF230527 Genomic RNA. Translation: AAG40592.1.
    AF230528 Genomic RNA. Translation: AAG40593.1.
    AF339836 Genomic RNA. Translation: AAK14397.1.
    AF220295 Genomic RNA. Translation: AAL40399.1.
    PIRiA31684. HMIHBQ.
    RefSeqiNP_150076.1. NC_003045.1.

    Genome annotation databases

    GeneIDi921684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38962 Genomic RNA. Translation: AAA92989.1 .
    L38963 Genomic RNA. Translation: AAA92990.1 .
    U06093 Genomic RNA. Translation: AAA92991.1 .
    AF239306 Genomic RNA. Translation: AAG40594.1 .
    AF239307 Genomic RNA. Translation: AAG40600.1 .
    AF230523 Genomic RNA. Translation: AAG40588.1 .
    AF230524 Genomic RNA. Translation: AAG40589.1 .
    AF230525 Genomic RNA. Translation: AAG40590.1 .
    AF230526 Genomic RNA. Translation: AAG40591.1 .
    AF230527 Genomic RNA. Translation: AAG40592.1 .
    AF230528 Genomic RNA. Translation: AAG40593.1 .
    AF339836 Genomic RNA. Translation: AAK14397.1 .
    AF220295 Genomic RNA. Translation: AAL40399.1 .
    PIRi A31684. HMIHBQ.
    RefSeqi NP_150076.1. NC_003045.1.

    3D structure databases

    ProteinModelPortali P59709.
    SMRi P59709. Positions 19-376.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 921684.

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and in vitro expression of the gene for the E3 haemagglutinin glycoprotein of bovine coronavirus."
      Parker M.D., Cox G.J., Deregt D., Fitzpatrick D.R., Babiuk L.A.
      J. Gen. Virol. 70:155-164(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Comparison of bovine coronavirus isolates associated with neonatal calf diarrhoea and winter dysentery in adult dairy cattle in Quebec."
      Dea S., Michaud L., Milane G.
      J. Gen. Virol. 76:1263-1270(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate BCQ.2590, Isolate BCQ.3 and Isolate BCQ.571.
    3. "Identification of specific variations within the HE, S1, and ORF4 genes of bovine coronaviruses associated with enteric and respiratory diseases in dairy cattle."
      Gelinas A.-M., Sasseville A.M.-J., Dea S.
      Adv. Exp. Med. Biol. 494:63-67(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate BCQ.1523, Isolate BCQ.2590, Isolate BCQ.3994, Isolate BCQ.571 and Isolate BCQ.7373.
    4. "Genomic and antigenic variations of the HE glycoprotein of bovine coronaviruses associated with neonatal calf diarrhea and winter dysentery."
      Kourtesis A.B., Gelinas A.-M., Dea S.
      Arch. Virol. 146:1219-1230(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.3708, Isolate BCQ.376, Isolate BCQ.701 and Isolate BCQ.7373.
    5. "Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein."
      Gelinas A.-M., Boutin M., Sasseville A.M.-J., Dea S.
      Virus Res. 76:43-57(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate BCQ.3994.
    6. "Full-length genomic sequence of bovine coronavirus (31 kb). Completion of the open reading frame 1a/1b sequences."
      Yoo D., Pei Y.
      Adv. Exp. Med. Biol. 494:73-76(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_CVBQ
    AccessioniPrimary (citable) accession number: P59709
    Secondary accession number(s): P24351
    , Q66166, Q66167, Q66168, Q77NC5, Q98VL2, Q9DGT1, Q9DGT9, Q9DR84, Q9DRF4, Q9DRF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: June 16, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sequence shown is that of Quebec reference strain.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3