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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Bovine coronavirus (strain Quebec) (BCoV) (BCV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401NucleophileBy similarity
Active sitei326 – 3261Charge relay systemBy similarity
Active sitei329 – 3291Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiBovine coronavirus (strain Quebec) (BCoV) (BCV)
Taxonomic identifieri11133 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000008572 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 392374Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence AnalysisAdd
BLAST
Topological domaini414 – 42411IntravirionSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 424406Hemagglutinin-esterasePRO_0000037143Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 65By similarity
Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi89 – 891N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi113 ↔ 162By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi197 ↔ 276By similarity
Disulfide bondi205 ↔ 249By similarity
Glycosylationi236 – 2361N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi307 ↔ 312By similarity
Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi347 ↔ 371By similarity
Glycosylationi358 – 3581N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliP59709.
SMRiP59709. Positions 19-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 127121Esterase domain first partBy similarityAdd
BLAST
Regioni128 – 266139Receptor bindingBy similarityAdd
BLAST
Regioni267 – 379113Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19253.

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLLRFVLV SCIIGSLGFD NPPTNVVSHL NGDWFLFGDS RSDCNHVVNT
60 70 80 90 100
NPRNYSYMDL NPALCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY
110 120 130 140 150
EGLNFTPYHA FKCTTSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY
160 170 180 190 200
VYNGSAQSTA LCKSGSLVLN NPAYIAREAN FGDYYYKVEA DFYLSGCDEY
210 220 230 240 250
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDFNCH
260 270 280 290 300
YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD
310 320 330 340 350
NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYDSPCFSQ
360 370 380 390 400
QGVFRYDNVS SVWPLYSYGR CPTAADINTP DVPICVYDPL PLILLGILLG
410 420
VAVIIIVVLL LYFMVDNGTR LHDA
Length:424
Mass (Da):47,709
Last modified:June 16, 2003 - v1
Checksum:iF1CE076E4AA7B277
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti5 – 51L → P in strain: Isolate BCQ.3, Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.2590, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
Natural varianti8 – 81V → A in strain: Isolate BCQ.2590.
Natural varianti11 – 111S → C in strain: Isolate BCQ.1523, Isolate BCQ.2442 and Isolate BCQ.2508.
Natural varianti49 – 491N → T in strain: Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
Natural varianti53 – 531R → P in strain: Isolate BCQ.2590.
Natural varianti66 – 661D → G in strain: Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.3708 and Isolate BCQ.3994.
Natural varianti103 – 1031L → I in strain: Isolate BCQ.2439.
Natural varianti103 – 1031L → V in strain: Isolate BCQ.3, Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.2590, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
Natural varianti114 – 1141T → I in strain: Isolate BCQ.3708.
Natural varianti182 – 1821G → R in strain: Isolate BCQ.7373.
Natural varianti245 – 2451F → L in strain: Isolate BCQ.7373.
Natural varianti282 – 2821D → G in strain: Isolate BCQ.7373.
Natural varianti344 – 3441D → A in strain: Isolate BCQ.2590.
Natural varianti350 – 3501Q → R in strain: Isolate BCQ.2590.
Natural varianti367 – 3671S → P in strain: Isolate BCQ.376, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.2590, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.
Natural varianti392 – 3921L → I in strain: Isolate BCQ.3, Isolate BCQ.376, Isolate BCQ.571, Isolate BCQ.701, Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.3708, Isolate BCQ.3994 and Isolate BCQ.7373.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38962 Genomic RNA. Translation: AAA92989.1.
L38963 Genomic RNA. Translation: AAA92990.1.
U06093 Genomic RNA. Translation: AAA92991.1.
AF239306 Genomic RNA. Translation: AAG40594.1.
AF239307 Genomic RNA. Translation: AAG40600.1.
AF230523 Genomic RNA. Translation: AAG40588.1.
AF230524 Genomic RNA. Translation: AAG40589.1.
AF230525 Genomic RNA. Translation: AAG40590.1.
AF230526 Genomic RNA. Translation: AAG40591.1.
AF230527 Genomic RNA. Translation: AAG40592.1.
AF230528 Genomic RNA. Translation: AAG40593.1.
AF339836 Genomic RNA. Translation: AAK14397.1.
AF220295 Genomic RNA. Translation: AAL40399.1.
PIRiA31684. HMIHBQ.
RefSeqiNP_150076.1. NC_003045.1.

Genome annotation databases

GeneIDi921684.
KEGGivg:921684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38962 Genomic RNA. Translation: AAA92989.1.
L38963 Genomic RNA. Translation: AAA92990.1.
U06093 Genomic RNA. Translation: AAA92991.1.
AF239306 Genomic RNA. Translation: AAG40594.1.
AF239307 Genomic RNA. Translation: AAG40600.1.
AF230523 Genomic RNA. Translation: AAG40588.1.
AF230524 Genomic RNA. Translation: AAG40589.1.
AF230525 Genomic RNA. Translation: AAG40590.1.
AF230526 Genomic RNA. Translation: AAG40591.1.
AF230527 Genomic RNA. Translation: AAG40592.1.
AF230528 Genomic RNA. Translation: AAG40593.1.
AF339836 Genomic RNA. Translation: AAK14397.1.
AF220295 Genomic RNA. Translation: AAL40399.1.
PIRiA31684. HMIHBQ.
RefSeqiNP_150076.1. NC_003045.1.

3D structure databases

ProteinModelPortaliP59709.
SMRiP59709. Positions 19-376.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi921684.
KEGGivg:921684.

Phylogenomic databases

KOiK19253.

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and in vitro expression of the gene for the E3 haemagglutinin glycoprotein of bovine coronavirus."
    Parker M.D., Cox G.J., Deregt D., Fitzpatrick D.R., Babiuk L.A.
    J. Gen. Virol. 70:155-164(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Comparison of bovine coronavirus isolates associated with neonatal calf diarrhoea and winter dysentery in adult dairy cattle in Quebec."
    Dea S., Michaud L., Milane G.
    J. Gen. Virol. 76:1263-1270(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BCQ.2590, Isolate BCQ.3 and Isolate BCQ.571.
  3. "Identification of specific variations within the HE, S1, and ORF4 genes of bovine coronaviruses associated with enteric and respiratory diseases in dairy cattle."
    Gelinas A.-M., Sasseville A.M.-J., Dea S.
    Adv. Exp. Med. Biol. 494:63-67(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BCQ.1523, Isolate BCQ.2590, Isolate BCQ.3994, Isolate BCQ.571 and Isolate BCQ.7373.
  4. "Genomic and antigenic variations of the HE glycoprotein of bovine coronaviruses associated with neonatal calf diarrhea and winter dysentery."
    Kourtesis A.B., Gelinas A.-M., Dea S.
    Arch. Virol. 146:1219-1230(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BCQ.1523, Isolate BCQ.2439, Isolate BCQ.2442, Isolate BCQ.2508, Isolate BCQ.3708, Isolate BCQ.376, Isolate BCQ.701 and Isolate BCQ.7373.
  5. "Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein."
    Gelinas A.-M., Boutin M., Sasseville A.M.-J., Dea S.
    Virus Res. 76:43-57(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate BCQ.3994.
  6. "Full-length genomic sequence of bovine coronavirus (31 kb). Completion of the open reading frame 1a/1b sequences."
    Yoo D., Pei Y.
    Adv. Exp. Med. Biol. 494:73-76(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_CVBQ
AccessioniPrimary (citable) accession number: P59709
Secondary accession number(s): P24351
, Q66166, Q66167, Q66168, Q77NC5, Q98VL2, Q9DGT1, Q9DGT9, Q9DR84, Q9DRF4, Q9DRF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence shown is that of Quebec reference strain.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.