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Protein

Splicing factor 3B subunit 6

Gene

Sf3b6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • blastocyst formation Source: MGI
  • mRNA splicing, via spliceosome Source: MGI
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 6
Alternative name(s):
Pre-mRNA branch site protein p14
SF3b 14 kDa subunit
Gene namesi
Name:Sf3b6
Synonyms:Sf3b14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1913305. Sf3b6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125Splicing factor 3B subunit 6PRO_0000081726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291N6-acetyllysine; alternateBy similarity
Cross-linki29 – 29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei41 – 411N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP59708.
MaxQBiP59708.
PaxDbiP59708.
PeptideAtlasiP59708.
PRIDEiP59708.

PTM databases

iPTMnetiP59708.
PhosphoSiteiP59708.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037361.
CleanExiMM_0610009D07RIK.
GenevisibleiP59708. MM.

Interactioni

Subunit structurei

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. Interacts with SF3B1. Interacts to a lesser extent with SF3B3. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.

Protein-protein interaction databases

BioGridi211182. 1 interaction.
IntActiP59708. 2 interactions.
MINTiMINT-1868268.
STRINGi10090.ENSMUSP00000043662.

Structurei

3D structure databases

ProteinModelPortaliP59708.
SMRiP59708. Positions 12-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 9476RRMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 2914Interaction with pre-mRNA branch siteBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0114. Eukaryota.
ENOG4111IHX. LUCA.
GeneTreeiENSGT00390000005908.
HOGENOMiHOG000276232.
HOVERGENiHBG039634.
InParanoidiP59708.
KOiK12833.
OMAiVNRVLFV.
OrthoDBiEOG091G0RTZ.
PhylomeDBiP59708.
TreeFamiTF314161.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV
60 70 80 90 100
GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK
110 120
MDTKKKEEQL KLLKEKYGIN TDPPK
Length:125
Mass (Da):14,585
Last modified:June 6, 2003 - v1
Checksum:iA1439DACB78A3208
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002375 mRNA. Translation: BAB22052.1.
AK003683 mRNA. Translation: BAB22936.1.
AK151648 mRNA. Translation: BAE30577.1.
BC019535 mRNA. Translation: AAH19535.1.
CCDSiCCDS25791.1.
RefSeqiNP_079599.1. NM_025323.2.
UniGeneiMm.102627.

Genome annotation databases

EnsembliENSMUST00000046207; ENSMUSP00000043662; ENSMUSG00000037361.
GeneIDi66055.
KEGGimmu:66055.
UCSCiuc007myi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002375 mRNA. Translation: BAB22052.1.
AK003683 mRNA. Translation: BAB22936.1.
AK151648 mRNA. Translation: BAE30577.1.
BC019535 mRNA. Translation: AAH19535.1.
CCDSiCCDS25791.1.
RefSeqiNP_079599.1. NM_025323.2.
UniGeneiMm.102627.

3D structure databases

ProteinModelPortaliP59708.
SMRiP59708. Positions 12-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211182. 1 interaction.
IntActiP59708. 2 interactions.
MINTiMINT-1868268.
STRINGi10090.ENSMUSP00000043662.

PTM databases

iPTMnetiP59708.
PhosphoSiteiP59708.

Proteomic databases

EPDiP59708.
MaxQBiP59708.
PaxDbiP59708.
PeptideAtlasiP59708.
PRIDEiP59708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046207; ENSMUSP00000043662; ENSMUSG00000037361.
GeneIDi66055.
KEGGimmu:66055.
UCSCiuc007myi.1. mouse.

Organism-specific databases

CTDi51639.
MGIiMGI:1913305. Sf3b6.

Phylogenomic databases

eggNOGiKOG0114. Eukaryota.
ENOG4111IHX. LUCA.
GeneTreeiENSGT00390000005908.
HOGENOMiHOG000276232.
HOVERGENiHBG039634.
InParanoidiP59708.
KOiK12833.
OMAiVNRVLFV.
OrthoDBiEOG091G0RTZ.
PhylomeDBiP59708.
TreeFamiTF314161.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

PROiP59708.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037361.
CleanExiMM_0610009D07RIK.
GenevisibleiP59708. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSF3B6_MOUSE
AccessioniPrimary (citable) accession number: P59708
Secondary accession number(s): Q3U9T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2003
Last sequence update: June 6, 2003
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.